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Sex-Based Variation of Gene Expression in the Gonads and Fins of Russian Sturgeon (<i>Acipenser gueldenstaedtii</i>) 被引量:1
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作者 Gad Degani Akram Hajouj Avshalom Hurvitz 《Open Journal of Animal Sciences》 2021年第1期1-10,共10页
Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></spa... Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;">) is a primitive freshwater fish and a source of black caviar.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these ge</span><span style="font-family:Verdana;">nes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old;sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fin</span><span style="font-family:Verdana;">s. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal</span><span style="font-family:Verdana;"> tissue and the need for genetic sex markers in the Russian sturgeon. 展开更多
关键词 ACIPENSER Gene Expression Sexual Maturation gonads Sex Markers
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Localization of nitric oxide synthase in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense 被引量:2
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作者 WANG Yongjun ZHANG Shicui 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2005年第5期120-126,共7页
The localization of nitric oxide synthases (NOS) is dealed with in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense. It was found by NADPH-diaphorase staining that (1) NOS activity was presen... The localization of nitric oxide synthases (NOS) is dealed with in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense. It was found by NADPH-diaphorase staining that (1) NOS activity was present in the nuclear membranes of germinal vesicles during the entire period ofoocyte development; (2) NOS was localized in both the nuclear membranes and the perinuclear region of cytoplasm in the vitellogenetic oocytes; (3) NOS was relocated in the cortical layer in the mature egg; (4) NOS activity was present in spermatocytes, but not in the spermatogonia in the middle of October; (5) NOS was detected in both spermatozoa and spermatids as well as spermatocytes during the breeding season. This is the first report on the distribution pattern of NOS in the developing gonads in protochordates. These results suggest a role for NOS in the functioning of the nuclear membranes and yolk synthesis during oogenesis and in cell division and differentiation during spermatogenesis. 展开更多
关键词 AMPHIOXUS BRANCHIOSTOMA nitric oxide synthase GONAD LOCALIZATION
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Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole(Cynoglossus semilaevis) 被引量:1
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作者 Yi-Fang Lu Qian Liu +4 位作者 Kai-Qiang Liu Hong-Yan Wang Cheng-Hua Li Qian Wang Chang-Wei Shao 《Zoological Research》 SCIE CAS CSCD 2022年第3期319-330,共12页
Chinese tongue sole(Cynoglossus semilaevis)is an economically important marine fish species with a ZZ/ZW sex determination mechanism,which can be influenced by temperature.Alternative splicing(AS)is an important mecha... Chinese tongue sole(Cynoglossus semilaevis)is an economically important marine fish species with a ZZ/ZW sex determination mechanism,which can be influenced by temperature.Alternative splicing(AS)is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation,but has rarely been reported in fish.In this study,to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation,we combined isoform and RNA sequencing(Iso-Seq and RNA-Seq)to perform transcriptome profiling of male and female gonads in C.semilaevis.In total,81883 and 32341 full-length transcripts were obtained in males and females,respectively.A total of 8279 AS genes were identified,including 2639 genes showing differential AS(DAS)between males and females.Many intersecting DAS genes and differentially expressed genes(DEGs)were enriched in the meiotic cell cycle pathway,and genes related to gonadal differentiation,such as esrrb and wt1a,were found to have sex-specific isoforms.Thus,this study revealed AS events in the gonadal transcriptomes of male and female C.semilaevis,described the characteristics of active transcription in the testes,and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation. 展开更多
关键词 Gonadal differentiation Alternative splicing Sex-specific splicing Cynoglossus semilaevis Iso-Seq RNA-SEQ
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PATHOLOGICAL CHARACTERISTICS OF GONADS IN NINE PATIENTS WITH TRUE HERMAPHRODITISM
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作者 郭丽娜 刘彤华 田秦杰 《Chinese Medical Sciences Journal》 CAS CSCD 1998年第2期103-106,共4页
The gonadal histopathology, and its correlation with the clinical features has been investigated in 9 true hermaphroditism patients, aged 5-21 yr. Seven of 9 patients had been raised as females, of which the chromosom... The gonadal histopathology, and its correlation with the clinical features has been investigated in 9 true hermaphroditism patients, aged 5-21 yr. Seven of 9 patients had been raised as females, of which the chromosomal karyotype was 46XX in 5 cases, 46XX/46XY and 46XX/47XXY in 2 cases. Two of 9 pa- tients were raised as males, the chromosomal karyotypes being 46XX and 46XX/46XY. All 9 patients had testicular tissue excised, and biopsies of the conserved ovarian tissue were performed. Ovotestis was the most common form of the abnormal gonads; two of 9 patients had bilateral ovotestes, seven had unilateral ovotestes (5 in right side, 2 in left side). In seven patients with a unilateral ovotestis, 6 had a contralateral ovary and one had a contralateral testis. Microscopically, the ovarian tissue of 11 ovotestes, including 6 biopsies from contealateral ovaris, were normal, with many primordal follicles and a few growing follicles. In two of the patients, aged over 15 years, evidence of ovulation was observed. In comparison, the testicu- lar tissue of the ovotestis and the one left inguinal testis was histologically abnormal, with immature semi- niferous tubules, most of which filled with Sertoli cells only. Three of 9 patients married after surgical treatment. Two of these subsequently conceived and delivered of normal infants by cesarean section. 展开更多
关键词 true hermaphroditism GONAD HISTOPATHOLOGY
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Histological Observation of Gonads of Male Elk (Elaphurus davidianus)
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作者 Xiang Jinmei Chen Sen Wan Chunyun 《Animal Husbandry and Feed Science》 CAS 2019年第2期84-86,共3页
Elk (Elaphurus davidianus) is a rare species native to China, and much less efforts have been dedicated to gonadal histology of male elk.The microscopic morphology of gonadal tissue of elk was observed by paraffin met... Elk (Elaphurus davidianus) is a rare species native to China, and much less efforts have been dedicated to gonadal histology of male elk.The microscopic morphology of gonadal tissue of elk was observed by paraffin method. The results showed that the tunica albuginea testis was com-posed of collagenous fibers and elastic fibers, with the thickness of about 1 054 μm, and a large number of blood vessels were distributed in tunica albuginea;the seminiferous tubule was about 203 μm in diameter, with uneven thickness of tubule wall, which was consisted of sustentacular cells and spermatogenic cells;there were interstitial cells and blood vessels between seminiferous tubules. The epididymis contained more than 20 effer-ent ducts and a long curly epididymal duct;the capsule thickness at epididymis head was about 53 μm, and that at epididymis body and tail was400-1 000 μm;the epithelium of efferent duct was pseudostratified columnar epithelial cells;the wall epithelium of epididymal duct was composed of tall columnar cells at proximal lumina and stroma cells at distal lumina;the epithelial basement membrane of efferent duct and epididymal duct contained circular smooth muscle. The wall of spermatic duct was divided into mucosae, tunica muscularis and tunica adventitia. The tunica muscu-laris was well developed, which was smooth muscle fiber. 展开更多
关键词 GONAD HISTOLOGICAL MORPHOLOGY ELK
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Odor-Active Volatile Compounds from the Gonads of the Sea Urchin <i>Mesocentrotus nudus</i>in the Wild in Miyagi Prefecture, Tohoku, Japan
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作者 Yoichi Sato Satomi Takagi +1 位作者 Eri Inomata Yukio Agatsuma 《Food and Nutrition Sciences》 2019年第7期860-875,共16页
Background and Aims: Mesocentrotus nudus is commercially harvested from shallow rocky bottoms in northern Japan. The gonads are targeted as an edible product. The objective of this study was to identify odor-active vo... Background and Aims: Mesocentrotus nudus is commercially harvested from shallow rocky bottoms in northern Japan. The gonads are targeted as an edible product. The objective of this study was to identify odor-active volatile organic compounds (VOCs) from the gonads of adult M. nudus collected at a fishing ground in Miyagi Prefecture, Tohoku, Japan. Methods and Results: Gas chromatography-mass spectrometry analysis and gas chromatography-sniffing techniques identified 42 compounds categorized as alcohols, aldehydes, aromatic hydrocarbons, esters, halomethanes, hydrocarbons, ketones, sulfur-containing compounds, and nitrogen-containing compounds. GC-sniffing analysis characterized four compounds with preferable odors of sea urchin gonads;limonene, propyl acetate, acetone, dibromochloromethane. On the other hand, the analysis characterized three compounds with unpreferable odors;methyl mercaptane, dimethyl sulfide, and s-methyl thioacetate. Several VOCs from the gonads were derived from seaweeds, terrestrial plants, and fish flesh as food because M. nudus is omnivorous. Conclusion: This is the first study to identify VOCs from edible sea urchin gonads in the wild in Japan. These VOC data comprise a typical standard in order to evaluate a higher quality of sea urchin gonads. 展开更多
关键词 Sea URCHIN Mesocentrotus nudus GONAD FLAVOR GC/MS SNIFFING Analysis
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Germ cell development in teleost gonads
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作者 Partigya Sharma Shriya Purohit +1 位作者 Sachin Kothiyal Indrashis Bhattacharya 《Aquaculture and Fisheries》 CSCD 2024年第3期422-436,共15页
Among vertebrate species,fishes are considered to be the most developmentally diverse taxa exhibiting remarkable gonadal plasticity with capacity of sex reversal.Sex determination in teleost fishes is governed via a c... Among vertebrate species,fishes are considered to be the most developmentally diverse taxa exhibiting remarkable gonadal plasticity with capacity of sex reversal.Sex determination in teleost fishes is governed via a complex regulatory network involving multiple genetic,environmental(temperature,population density,pH),social interaction/stress factors.Piscine germ-line originates as Primordial germ cells(PGC)from cleavage blastomeres/endoderm and gets specified by maternal inheritance of Vasa,N anos,Tdrds,Dnd,Dazl etc and subsequently migrates to reach the embryonic gonadal primordium.Post-hatching,mitotically active germ cells(Gc)continue to proliferate via two distinct pathways-firstly,stem cell-like mode followed by meiosis-committed cystic division.In Japanese rice fish medaka,Oryzias latipes sex gets determined on the chromosomal basis,where XY becomes a male because of testicular Dmy transcription and XX develops as female owing to Gc restricted Foxl 3 expression.Sex specific germ-line stem cell systems also have been found in adult O.latipes.Intriguingly in teleost fishes,the developmental fate of transplanted Gc do not depend on the sex of the donor rather solely relies upon the sex of the recipient.Furthermore,sexually mature piscine gonads remain developmentally plastic showing extensive responsiveness towards sex steroids.The critical regulation of gonadal differentiation is directed by female specific 17β-estradiol(E2)and bio-active male androgen 11-keto-testosretone(11-kT).Various transcription factors(TFs)e.g.-Dmrt1,DAX1,Ad4BP/SF-1,Foxl 2 regulate the bio-conversion rate of testosterone(T)to either E2 or 11-kT.Similarly,multiple endocrine factors(EFs)e.g.-LH,FSH,E2,11-kT and growth factors(GFs)e.g-AMH,GSDF,IGF-1/3,GDF-9/BMP-15 critically induce Gc differentiation to regulate the gametogenic output.In this article we have precisely discussed the endocrine regulation of Gc development during gonadal maturation of teleosts. 展开更多
关键词 TESTIS SPERMATOGENESIS SPERM OVARY OOGENESIS Egg gonads
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Immunohistochemical localization of gonadotropin-releasing hormone receptors (GnRHR) in the nervous system,Hatschek's pit and gonads of amphioxus,Branchiostoma belcheri 被引量:7
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作者 FANG Yongqiang, HUANG Weiquan and CHEN Lei The Third Institute of Oceanography, State Ocean Administration , Xiamen 361005 , China The Fourth Military Medical College, Xi’ an 710032, China 《Chinese Science Bulletin》 SCIE EI CAS 1999年第10期908-912,共5页
Using gonadotropin-releasing hormone (GnRH) anti-idiotypic antibodies and APA immunohistochemical method, the immunoreactivity of GnRHR in the nervous system, Hatschek’s pit and gonads of amphioxus has been located. ... Using gonadotropin-releasing hormone (GnRH) anti-idiotypic antibodies and APA immunohistochemical method, the immunoreactivity of GnRHR in the nervous system, Hatschek’s pit and gonads of amphioxus has been located. It is found for the first time that the immunoreactivity of GnRHR exists in the nerve cells and fibers in the amphioxus’s brain and nerve tube and the epithelial cells of Hatschek’s pit at the different stages of gonadal development. At the same time, it is also found that GnRHR also exists in the ovary and testis of different developed stages. These findings provide morphological new proof for the informative transfer and regulation between brain and Hatschek’s pit mediation by GnRHR, and for the understanding of the mechanism of action on the reproductive endocrine control axis among brain-Hatschek’s pit-gonads. 展开更多
关键词 amphioxus. GnRHR immunohistochemistry. nervous SYSTEM gonads Hatschek’s pit.
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Fatty acids in female’s gonads of the Red Sea fish Rhabdosargus sarba during the spawning season
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作者 Suhaila Qari Samia Moharram Safaa Alowaidi 《Journal of Coastal Life Medicine》 2014年第5期411-415,共5页
Objective:To determine the fatty acids profiles in female fish,Rhabdosargus sarba(R.sarba)from the Red Sea during the spawning season.Methods:Monthly individual R.sarba were obtained from Bangalah market in Jeddah,Red... Objective:To determine the fatty acids profiles in female fish,Rhabdosargus sarba(R.sarba)from the Red Sea during the spawning season.Methods:Monthly individual R.sarba were obtained from Bangalah market in Jeddah,Red Sea and transported to the laboratory in ice aquarium.The total length,standard length and weight were measured,fishes were dissected.Ovaries were removed,weighed and 10 mL of concentrated hydrochloric acid were added to 10 g of the ovary in a conical flask and immersed in boiling water until the sample was dissolved and the fat was seen to collect on the surface.The conical was cooled and the fat was extracted by shaking with 30 mL of diethyl ether.The extract was bowled after allowing the layers to separate into a weighed flask.The extraction was repeated three times more and distilled off the solvent then the fat dried at 100℃,cooled and weighed.Then 50 mg of lipid was put in a tube,5 mL of methanolic sulphuric acid was added and 2 mL of benzene,the tube well closed and placed in water bath at 90℃for an hour and a half.After cooling,8 mL water and 5 mL petroleum were added and shaked strongly and the ethereal layer was separated in a dry tube,evaporated to dryness.The fatty acid methyl esters were analyzed by using a Hewlett Packard(HP 6890)chromatography,a split/splitless injector and flame ionization detector.Results:In female R.sarba,a total of 29 fatty acids were detected in ovaries throughout the spawning season.The main fatty acid group in total lipid was saturated fatty acid(SFA,28.9%),followed by 23.5%of polyunsaturated fatty acids(PUFA)and 12.9%of monounsaturated fatty acids(MUFA).The dominant SFA were palmitic and stearic,the major MUFA were palmitoleic and oleic,and the major PUFA were C18:2 and C22:2.During spawning stages,there were no significant differences in total SFA,MUFA and PUFA.The highest value of SFA was in late spawning(36.78%).However,the highest value of MUFA and PUFA was in spawning(16.70%and 24.96%respectively).During spawning season there were significant differences in total SFA between March(late spawning stage)and December(nearly ripe stage),P<0.05.Conclusions:From the present study it has been shown that 29 fatty acids were detected in ovaries of R.sarba throughout the spawning season.The main fatty acid group in total lipid was SFA followed by PUFA and MUFA.The dominant SFA were palmitic and stearic,the major MUFA were palmitoleic and oleic,and the major PUFA were C18:2 and C22:2.The highest value of SFA was in late spawning.However,the highest value of MUFA and PUFA was in spawning. 展开更多
关键词 SPARIDAE Rhabdosargus sarba FISH Fatty acids SPAWNING gonads Red Sea
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Expression,localization,and a regulated target gene(ccnd1)of miR-202-5p in the Japanese flounder gonads
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作者 Fengfeng Shen Qinghe Chao +3 位作者 Zhenxi Cai Haoran Zhang Jikui Wu Junling Zhang 《Aquaculture and Fisheries》 CSCD 2023年第3期267-273,共7页
MiR-202-5p is identified as the maker microRNA(miRNA)of germplasm in fish.However,the role of miR-202-5p in gonadal development is still unclear.Here we investigated the expression patterns of miR-202-5p in Japanese f... MiR-202-5p is identified as the maker microRNA(miRNA)of germplasm in fish.However,the role of miR-202-5p in gonadal development is still unclear.Here we investigated the expression patterns of miR-202-5p in Japanese flounder(Paralichthys olivaceus).Real-time PCR displayed that miR-202-5p was specifically expressed in bisexual gonads,and its transcription in the testis was relatively abundant than that in the ovary.Moreover,it was gradually increased in testis from stage II to stage IV.Fluorescent in situ hybridization(FISH)illustrated that miR-202-5p was co-localized with vasa(a marker gene of germ cells)in testis,and it was predominately expressed in spermatogonia and spermatocytes,implying that miR-202-5p might participate in spermatogenesis.Considering the important role of retinoic acid(RA)on regulating meiosis during spermatogenesis,we treated Japanese flounders by injecting all-trans retinoic acid(ATRA).MiR-202-5p in testis was shown to be up-regulated,and ccnd1,a key cell cycle regulator,was found to be down-regulated after ATRA treatment.Furthermore,ccnd1 was demonstrated to be a direct regulatory target of miR-202-5p using Dual-luciferase assay.In the primary testis cell from Japanese flounder,we further confirmed that ccnd1 was down-regulated by miR-202-5p.The results suggest that miR-202-5p exerts a crucial role in gonadal development by inhibiting ccnd1 in Japanese flounder. 展开更多
关键词 Paralichthys olivaceus GONAD miR-202-5p CCND1
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Characterization and expression analysis of sox3 in medaka gonads
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作者 Quan Pu Yuan Ma +3 位作者 Ying Zhong Jing Guo Lang Gui Mingyou Li 《Aquaculture and Fisheries》 2022年第1期23-30,共8页
Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in vertebrates.However,the accurate expression pattern is still unclear in fish.In this study,medaka sox3 was isolated and its... Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in vertebrates.However,the accurate expression pattern is still unclear in fish.In this study,medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult gonads.By reverse transcription-polymerase chain reaction(RT-PCR)analysis,sox3 RNA expression was detected in the brain,eyes and gonads.By chromogenic and fluorescent in situ hybridization,sox3 was present in the oogonia and early stages of oocytes as well as granulosa cells and theca cells in the later stages;in the testis,sox3 was occurred in the Sertoli cells and sperm with a specific signal in the location of chromatid body of sperm;in the embryos,sox3 was expressed in the central nervous.These results suggest that medaka sox3 gene was occurred in somatic and germ cells in adult gonads of both sexes,involved in the process of spermatogenesis,as well as the development of the nervous system.This study provides a precise expression pattern of sox3 and suggests that sox3 may be involved in gonadal and nervous system development of fish. 展开更多
关键词 MEDAKA sox3 Gonad development Nervous system development
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Textile dyeing wastewater negatively influences the hematological profile and reproductive health of male Swiss albino mice
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作者 Taimur Islam Nusrat Binte Rafique +7 位作者 Mohosina Mou Dipu Roy Robius Sani Sadi Ziban Chandra Das Anup Kumar Talukder Minhaz Ahmed Mizanur Rahman Golam Haider 《Asian pacific Journal of Reproduction》 CAS 2024年第4期169-177,共9页
Objective:To determine the effects of textile dyeing industrial wastewater on the hematological parameters and reproductive health including histoarchitecture of male gonad(testes)of mice.Methods:Twenty-four Swiss alb... Objective:To determine the effects of textile dyeing industrial wastewater on the hematological parameters and reproductive health including histoarchitecture of male gonad(testes)of mice.Methods:Twenty-four Swiss albino mice at 4-weeks old were divided into four groups(n=6 per group).Mice of group 1 supplied with normal drinking water were served as the control group.Mice of group 2,3 and 4 were supplied normal drinking water mixed with textile dyeing wastewater at 5%,10% and 20% concentration,respectively.After completing 24 weeks of treatment,different hematological profile,weight of testes,gonadosomatic index(GSI),sperm concentration and morphology were measured.Moreover,histopathological changes in testes were examined.Results:Hematocrit value and hemoglobin concentrations were decreased in all groups of wastewater-treated mice compared to the control group.Likewise,weight of testes,GSI and sperm concentration were decreased significantly in wastewater-treated mice in comparison to the control group.The percentage of morphologically healthy epididymal sperm was significantly reduced in wastewater-treated mice.Histopathological examination revealed degenerative changes in seminiferous tubules,a smaller number of spermatogenic cells,elongation of seminiferous tubules and degenerative changes of seminiferous tubules in wastewater-treated mice.Conclusions:Textile dyeing wastewater has harmful effects on hematological profile and reproductive health of male mice. 展开更多
关键词 Textile dyeing wastewater gonads HEMATOLOGY HISTOPATHOLOGY Mice
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Engineering of ovarian tissue for ovarian dysfunctions:A review
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作者 Aliya Zhylkybekova Gulbakit KKoshmaganbetova +5 位作者 Myltykbay SRysmakhanov Nurgul Abdullayevna Abenova Nadiar Maratovich Mussin Asset Askerovich Kaliyev Mahdi Mahdipour Amin Tamadon 《Asian pacific Journal of Reproduction》 CAS 2024年第1期3-11,共9页
This review explores tissue engineering as a potential solution for reproductive health issues in women caused by genetic or acquired diseases,such as premature ovarian failure or oophorectomy.The loss of ovarian func... This review explores tissue engineering as a potential solution for reproductive health issues in women caused by genetic or acquired diseases,such as premature ovarian failure or oophorectomy.The loss of ovarian function can lead to infertility,osteoporosis,and cardiovascular disease.Hormone replacement therapy is a common treatment,but it has limitations and risks.The review focuses on two main approaches in tissue engineering:scaffold-based(3D printing,electrospinning,decellularization)and scaffold-free(stem cell transplantation,organoid cultivation).Both approaches show promise in preclinical studies for creating functional ovarian tissue.Challenges include vascularization,innervation,long-term function,and safety.Despite these challenges,tissue engineering offers a potential avenue for restoring fertility and hormone balance in women with ovarian dysfunction. 展开更多
关键词 Female gonads Tissue engineering ESTROGEN Reproductive system
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The m6A methylation regulates gonadal sex differentiation in chicken embryo 被引量:4
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作者 Jianbo Li Xiuan Zhang +5 位作者 Xiqiong Wang Congjiao Sun Jiangxia Zheng Junying Li Guoqiang Yi Ning Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第6期1657-1670,共14页
Background:As a ubiquitous reversible epigenetic RNA modification,N6-methyladenosine(m6A)plays crucial regulatory roles in multiple biological pathways.However,its functional mechanisms in sex determination and differ... Background:As a ubiquitous reversible epigenetic RNA modification,N6-methyladenosine(m6A)plays crucial regulatory roles in multiple biological pathways.However,its functional mechanisms in sex determination and differentiation during gonadal development of chicken embryos are not clear.Therefore,we established a transcriptome-wide m6A map in the female and male chicken left gonads of embryonic day 7(E7)by methylated RNA immunoprecipitation sequencing(MeRIP-seq)to offer insight into the landscape of m6A methylation and investigate the post-transcriptional modification underlying gonadal differentiation.Results:The chicken embryonic gonadal transcriptome was extensively methylated.We found 15,191 and 16,111 m6A peaks in the female and male left gonads,respectively,which were mainly enriched in the coding sequence(CDS)and stop codon.Among these m6A peaks,we identified that 1013 and 751 were hypermethylated in females and males,respectively.These differential peaks covered 281 and 327 genes,such as BMP2,SMAD2,SOX9 and CYP19A1,which were primarily associated with development,morphogenesis and sex differentiation by functional enrichment.Further analysis revealed that the m6A methylation level was positively correlated with gene expression abundance.Furthermore,we found that YTHDC2 could regulate the expression of sex-related genes,especially HEMGN and SOX9,in male mesonephros/gonad mingle cells,which was verified by in vitro experiments,suggesting a regulatory role of m6A methylation in chicken gonad differentiation.Conclusions:This work provided a comprehensive m6A methylation profile of chicken embryonic gonads and revealed YTHDC2 as a key regulator responsible for sex differentiation.Our results contribute to a better understanding of epigenetic factors involved in chicken sex determination and differentiation and to promoting the future development of sex manipulation in poultry industry. 展开更多
关键词 CHICKEN EMBRYO gonads m6A YTHDC2
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Characterization of Bacterial Community Associated with Four Organs of the Yesso Scallop (Patinopecten yessoensis) by High-Throughput Sequencing 被引量:2
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作者 MA Yuexin LI Ming +3 位作者 SUN Jingxian HAO Zhenlin LIANG Jun ZHAO Xuewei 《Journal of Ocean University of China》 SCIE CAS CSCD 2019年第2期493-500,共8页
We used Illumina high-throughput sequencing of PCR-amplified V3-V4 16 S rRNA gene regions to characterize bacterial communities associated with the adductor muscles, gills, gonads and intestines of the Yesso scallop(P... We used Illumina high-throughput sequencing of PCR-amplified V3-V4 16 S rRNA gene regions to characterize bacterial communities associated with the adductor muscles, gills, gonads and intestines of the Yesso scallop(Patinopecten yessoensis) from waters around Zhangzidao, Dalian, China. Overall, 421,276 optimized reads were classified as 25 described bacterial phyla and 308 genera. Firmicutes, Proteobacteria, Tenericutes, Bacteroidetes, Chlamydiae and Spirochaetae accounted for > 97% of the total reads in the four organs. The bacterial 16 S rDNA sequences assigned to Firmicutes and Proteobacteria were abundant in the adductor muscles, gills and gonads; while reads from Tenericutes were dominant in the intestines, followed by those from Firmicutes, Chlamydiae, Proteobacteria and Bacteroidetes. At the genus level, the dominant genera in the adductor muscles, gills and gonads appeared to be Bacillus, Enterococcus and Lactococcus, whereas Mycoplasma was dominant in the intestines. The relative abundances of Bacillus, Enterococcus, Lactococcus, Alkaliphilus, Raoultella, Paenibacillus and Oceanobacillus were significantly lower in the intestine than in the other three organs. Cluster analysis and principal coordinates analysis of the operational taxonomy units profile revealed significant differences in the bacterial community structure between the intestine and the other three organs. Taken together, these results suggest that scallops have intestine-specific bacterial communities and the adductor muscles, gills and gonads harbor similar communities. The difference in the bacterial community between organs may relate to unique habitats, surroundings, diet and their respective physiological functions. 展开更多
关键词 Bacterial community Patinopecten yessoensis ADDUCTOR muscles GILLS gonads INTESTINES HIGH-THROUGHPUT sequencing
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Seasonal variations in gonad morphology and hypothalamic GnRH-I and GnIH in Eurasian Tree Sparrow,a multi-brooded passerine 被引量:1
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作者 Limin Wang Ghulam Nabi +8 位作者 Li Zhang Dan Liu Mo Li Juyong Li Kaixin Shi Ibrahim M.Ahmad Yuefeng Wu John C.Wingfield Dongming Li 《Avian Research》 SCIE CSCD 2022年第3期369-378,共10页
The hypothalamic-pituitary-gonadal(HPG)axis ubiquitously regulates seasonal reproduction,following the rhythmicity of a suite of environmental cues.Birds display prominent seasonal variations in gonad size regulated b... The hypothalamic-pituitary-gonadal(HPG)axis ubiquitously regulates seasonal reproduction,following the rhythmicity of a suite of environmental cues.Birds display prominent seasonal variations in gonad size regulated by two hypothalamic peptides,gonadotropin-releasing hormone-1(GnRH-I)and gonadotropin-inhibiting hormone(GnIH).How multi-brooded avian species adjust GnRH-I and GnIH to regulate gonadal morphology seasonally remains largely unknown.Here,we studied the variations in the hypothalamic immunoreactivity(ir)of GnRH-I and GnIH,gonadal proliferation,and apoptosis in a typical multi-brooded species,the Eurasian Tree Sparrow(Passer montanus),across the pre-breeding(PB),first breeding(FB),second breeding(SB),pre-basic molt(PM),and wintering stages(WS).Our results showed that both sexes had higher preoptic area(POA)-GnRH-I-ir but lower paraventricular nucleus(PVN)-GnIH-ir neurons during the breeding stages(FB and SB)relative to other life-history stages,with no significant differences between two broods.The testes and ovaries were significantly larger during the breeding stages.Testicular volume increased during the two broods due to anincreased diameter of seminiferous tubules.Furthermore,there were more testicular apoptotic cells in PB and WS stages than in breeding stages,and in PB stage than in PM stage.Males had higher POA-GnRH-I expression than females during the breeding stages,but both sexes had comparable PVN-GnIH expression throughout the annual cycle.Both sexes of the sparrows may undergo a similar pattern of life-history stage-dependent variation in the hypothalamic GnRH-I,GnIH,and gonadal morphology,except that during breeding stages,males may display higher expression of POA-GnRH-I relative to females.The higher expression of POA-GnRH-I-ir in breeding male sparrows may be critical for male-dependent parental care. 展开更多
关键词 Eurasian Tree Sparrow gonads HYPOTHALAMUS Reproduction Seasonality
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Nutrient effects of broodstocks on the larvae in Patinopecten yessoensis
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作者 柏雨岑 张涛 +2 位作者 邱天龙 高燕 张晓芳 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第4期988-996,共9页
Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and t... Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries(Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning(BC) and that of larvae(LC) was assessed as well as the change in nutrient levels in ovaries after spawning(DC). Results indicate that the BC of total carbohydrate(7.66%) and total lipid(14.48%) in ovaries were significantly higher than in testes(5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower(61.76%) than in the testes(81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. P atinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae(LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae. 展开更多
关键词 Patinopecten yessoensis nutrient composition fatty acids LARVAE broodstocks gonads
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A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus:identification and characterization of lymphocyte antigen 75(Ly75/CD205)
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作者 YANG Yang LIU Qinghua +2 位作者 MA Daoyuan SONG Zongcheng LI Jun 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第4期1342-1348,共7页
Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker i... Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes. 展开更多
关键词 Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker
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Male sex determination: insights into molecular mechanisms 被引量:8
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作者 Kathryn McClelland Josephine Bowles Peter Koopman 《Asian Journal of Andrology》 SCIE CAS CSCD 2012年第1期164-171,180,共9页
Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. T... Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. The activation of the Y-linked gene Sry (sex- determining region Y) and its downstream target Sox9 (Sry box-containing gene 9) triggers testis differentiation by stimulating the differentiation of Sertoli cells, which then direct testis morphogenesis. Once engaged, a genetic pathway promotes the testis development while actively suppressing genes involved in ovarian development. This review focuses on the events of testis determination and the struggle to maintain male fate in the face of antagonistic pressure from the underlying female programme. 展开更多
关键词 gonadal KNOCKOUT MICE sex determination sex-determining region Y protein sex disorders sex reversal sexual development TESTIS
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Sex-biased autophagy as a potential mechanism mediating sex differences in ischemic stroke outcome 被引量:4
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作者 Brian Noh Louise D.McCullough Jose F.Moruno-Manchon 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第1期31-37,共7页
Stroke is the second leading cause of death and a major cause of disability worldwide,and biological sex is an important determining factor in stroke incidence and pathology.From childhood through adulthood,men have a... Stroke is the second leading cause of death and a major cause of disability worldwide,and biological sex is an important determining factor in stroke incidence and pathology.From childhood through adulthood,men have a higher incidence of stroke compared with women.Abundant research has confirmed the beneficial effects of estrogen in experimental ischemic stroke but genetic factors such as the X-chromosome complement can also play an important role in determining sex differences in stroke.Autophagy is a self-degrading cellular process orchestrated by multiple core proteins,which leads to the engulfment of cytoplasmic material and degradation of cargo after autophagy vesicles fuse with lysosomes or endosomes.The levels and the activity of components of these signaling pathways and of autophagy-related proteins can be altered during ischemic insults.Ischemic stroke activates autophagy,however,whether inhibiting autophagy after stroke is beneficial in the brain is still under a debate.Autophagy is a potential mechanism that may contribute to differences in stroke progression between the sexes.Furthermore,the effects of manipulating autophagy may also differ between the sexes.Mechanisms that regulate autophagy in a sex-dependent manner in ischemic stroke remain unexplored.In this review,we summarize clinical and pre-clinical evidence for sex differences in stroke.We briefly introduce the autophagy process and summarize the effects of gonadal hormones in autophagy in the brain and discuss X-linked genes that could potentially regulate brain autophagy.Finally,we review pre-clinical studies that address the mechanisms that could mediate sex differences in brain autophagy after stroke. 展开更多
关键词 AUTOPHAGY brain ESTROGEN gonadal hormones NEURODEGENERATION neuron stroke X-CHROMOSOME
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