Rheological Behavior of Aqueous Solutions of Cationic Guar in Presence of Oppositely Charged Surfactant

The cationic guar （CG） is synthesized and the rheological behavior of aqueous solutions of CG in the presence of sodium dodecyl sulfate （SDS） is studied in detail. The steady viscosity measurements show that the zero shear viscosity enhancement can be almost 3 orders of magnitude as the concentration of SDS increases from 0 to 0.043%. The gel-like formation is observed as the concentration of SDS is greater than 0.016%. The oscillatory rheological measurements of CG solutions in the presence of SDS show that the crossover modulus is almost independent of the concentration of SDS whereas the apparent relaxation time increases swiftly upon increasing the concentration of SDS. The experimental results indicate that the strength rather than the number of the cross-links is greatly affected by SDS molecules. The mechanism concerning the effect of SDS upon the rheology of CG solutions can be coined by the two-stage model. Before the formation of cross-links at the critical concentration, the electrostatic interaction between SDS and cationic site of CG chains plays a key role and the SDS molecules bind to CG chains through the electrostatic interaction. After the formation of cross-links at the concentration greater than the critical concentration, the cooperative hydrophobic interaction become dominant and SDS molecules bind to the cross-links through the hydrophobic interaction. The theological behavior of aqueous solutions of CG in the presence of SDS is chiefly determined by the micelle-like cross-links between CG chains. In fact, the flow activation energy of CG solution, obtained from the temperature dependence of the apparent relaxation time, falls in the range of transferring a hydrophobic tail of SDS from the micelle to an aqueous environment.

Synthesis of Hydroxypropyl Guar Gum by Phase Transfer Catalysis

HGG (Hydroxypropyl guar gum) was synthesized by phase transfer catalysis for the first time. The effects of alkalinity, phase transfer catalyst, etherification, pH value, temperature, reaction time and stirring speed were investigated. An optimal synthetic reaction technology was established, namely, dose of guar gum is 100 g, propylene oxide 40-50 g, HTAC (hexadecyl trimethyl ammonium chloride ) 1.3-1.7 g, pH value 10-10.5, temperature 45-50℃, and reaction time 3-4 hours. The result shows that the improved HGG has high viscosity. Its dissolution speed, content of insoluble residue, colloid light transparency and stability are apparently superior to guar flour.

Effect of Potassium Oleate on Rheological Behavior of Cationic Guar in Aqueous Solution with Varying Temperatures

The rheology of the cationic guar （CG） solution was measured and the effects of potassium oleate （KOA） upon the rheological properties of CG solution were studied. The steady shear viscosity measurement has shown that the viscosity of CG solution increased dramatically in the presence of KOA. The viscosity enhancement of KOA upon CG solution can be approximate three orders in magnitude. The gel-like formation of CG solution is observed at the high concentration of KOA. The excess addition of KOA results in the phase separation of CG solution. The oscillatory rheological measurement has shown that the crossover modulus Gc （corresponding to either storage modulus G＇ or loss modulus G＇＇ at the frequency wc where G＇ equals G＇＇） for CG solution, decreases with the increasing the concentration of KOA in solution. On the other hand, the apparent relaxation time 7-app （=1/wc） increases with increasing the concentration of KOA in solution. Our experimental results suggest that for surfaetant such as KOA which has a stronger tendency to form micelles in solution, the cooperative hydrophobic interaction of polymer bound to surfactants is less necessary to the formation of aggregates in solution, especially at the high concentration of surfactants. In fact, with the increase of the concentration of KOA, the number of the aggregates which associate polymer together decreases whereas the intensity of these aggregates increases. The effect of temperature upon the aggregation is also significant. With the increase of temperature, the number of the aggregates increases whereas the intensity of these aggregates decreases, probably because the ionization of KOA increases at high temperature.

Partially hydrolyzed guar gum in pediatric functional abdominal pain

AIM:To assess the effects of partially hydrolyzed guar gum(PHGG) diet supplement in pediatric chronic abdominal pain(CAP) and irritable bowel syndrome(IBS).METHODS:A randomized,double-blind pilot study was performed in sixty children(8-16 years) with functional bowel disorders,such as CAP or IBS,diagnosed according to Rome Ⅲ criteria.All patients underwent ultrasound,blood and stool examinations to rule out any organic disease.Patients were allocated to receive PHGG at dosage of 5 g/d(n = 30) or placebo(fruitjuice n = 30) for 4 wk.The evaluation of the efficacy of fiber supplement included IBS symptom severity score(Birmingham IBS Questionnaire),severity of abdominal pain(Wong-Baker Face Pain Rating Score) and bowel habit(Bristol Stool Scale).Symptom scores were completed at 2,4,and 8 wk.The change from baseline in the symptom severity scale at the end of treatment and at 4 wk follow-up after treatment was the primary endpoint.The secondary endpoint was to evaluate compliance to supplementation with the PHGG in the pediatric population.Differences within groups during the treatment period and follow-up were evaluated by the Wilcoxon signed-rank test.RESULTS:The results of the study were assessed considering some variables,such as frequency and intensity of symptoms with modifications of the bowel habit.Both groups were balanced for baseline characteristics and all patients completed the study.Group A(PHGG group) presented a higher level of efficacy compared to group B(control group),(43% vs 5%,P = 0.025) in reducing clinical symptoms with modification of Birmingham IBS score(median 0 ± 1 vs 4 ± 1,P = 0.025),in intensity of CAP assessed with the Wong-Baker Face Pain Rating Score and in normalization of bowel habit evaluated with the Bristol Stool Scale(40% vs 13.3%,P = 0.025).In IBS subgroups,statistical analysis shown a tendency toward normalization of bowel movements,but there was no difference in the prevalence of improvement in two bowel habit subsets.PHGG was therefore better tolerated without any adverse effects.CONCLUSION:Although the cause of pediatric functional gastrointestinal disorders is not known,the results show that complementary therapy with PHGG may have beneficial effects on symptom control.

Selective depression behavior of guar gum on talc-type scheelite flotation

The depression behavior and mechanism of guar gum on talc-type scheelite flotation were systematically investigated by flotation experiments, adsorption tests, zeta-potential measurements, and infrared spectroscopic analyses. The flotation results for monominerals, mixed minerals, and actual mineral samples indicated that guar gum exhibited much higher selective depression for talc than for scheelite. Bench-scale closed-circuit tests showed that a tungsten concentrate with a WO_3 grade of 51.43% and a WO_3 recovery of 76.18% was obtained. Adsorption tests, zeta-potential measurements, and infrared spectral analyses confirmed that guar gum absorbed more strongly onto the talc surface than onto the scheelite surface because of chemisorption between guar gum and talc. This chemisorption is responsible for the guar gum's highly selective depression for talc and small depression for scheelite. The flotation results provide technical support for talc-type scheelite flotation.

Evaluation of flocculation characteristics of kaolinite dispersion system using guar gum:A green flocculant

This paper reports the systematic investigation on the flocculation,sedimentation and consolidation characteristics of kaolinite using guar gum as a green flocculant.In-situ flocculation behavior of kaolinite at various pH,guar gum dosages,and ionic strength were studied using a light scattering technique.The effect of these parameters on the settling rate,solid consolidation,and supernatant liquid clarity was recorded.The morphology of kaolinite and flocculated kaolinite aggregates were analyzed using FESEM.The morphology studies suggest that it is poorly crystalline with multiple steps on edge,broken edge;laminar with high aspect ratio and have rough basal surface.The complex irregularity on the basal surface and the presence of multiple steps in the edges,broken edges(hydroxyl groups)have facilitated the guar gum adsorption.The isoelectric point of kaolinite is pH 3.96.The pH,ionic strength and flocculant dosage have a significant effect on the kaolinite settling rate.The guar gum has exhibited excellent turbidity removal efficiency at pH 5.The turbidity removal is inefficient at pH 10.However,guar gum has shown high turbidity removal with 80%transmission at pH 10 in the presence of a KNO3 electrolyte.

Strength properties of xanthan gum and guar gum treated kaolin at different water contents

Nowadays,using biopolymer as a ground improvement method has become very popular.However,since biopolymers are organic and degradable,their long-term effect is not fully known.In this study,the effects of biopolymers on the mechanical behavior of kaolin clay were investigated through a comprehensive program of experiments.Two types of biopolymer,i.e.xanthan gum and guar gum were chosen to investigate the effect of biopolymer type.For this purpose,specimens were prepared using standard Proctor energy at four different water contents(25%,30%35%and 40%)with 0.5%,1%,1.5%and 2%biopolymer inclusions.The specimens were cured for 1 d,7 d,28 d and 90 d.Moreover,some of the specimens were kept in the curing room for 3 years to observe the long-term effect of the biopolymers.At the end of the curing periods,the specimens were subjected to unconfined compression test,and scanning electron microscopy(SEM)analysis was performed to observe the mechanism of strength improvement.The results revealed that the unconfined compressive strength(UCS)of the specimens treated with biopolymers increased in all biopolymer inclusion levels and water contents up to a 90-d curing period.For specimens containing xanthan gum,the maximum strength increase was observed at 25%water content and 2%xanthan gum with 90-d curing.The strength increased 5.23 times induced by xanthan gum addition when compared to the pure clay.Moreover,the increase in strength reached 8.53 times in specimens treated with guar gum.Besides,increasing water content caused more ductile behavior,thus increasing the axial deformation.

The selective effect of food-grade guar gum on chalcopyrite-monoclinic pyrrhotite separation using mixed aerofloat(CSU11) as collector

The flotation separation of chalcopyrite from monoclinic pyrrhotite using food-grade guar gum(FGG) as a depressant was studied through flotation tests, kinetic studies, dynamic potential measurements, adsorption experiments, and infrared spectral analyses. The microflotation results showed that the flotation separation of chalcopyrite from monoclinic pyrrhotite could not be realized by adding mixed aerofloat(CSU11) alone. The depressant FGG exhibited a selective depression effect on monoclinic pyrrhotite by controlling the pulp pH range from 5.0 to 6.0, with a maximum floatability variation of 79.36% in the presence of CSU11. The flotation kinetics, zeta-potential, adsorption, and infrared spectroscopy studies revealed that the FGG could absorb more strongly on the surface of monoclinic pyrrhotite than on the surface of chalcopyrite. In addition, the results revealed that the interaction of FGG with the monoclinic pyrrhotite surface was governed primarily by strong chemisorption, whereas FGG mainly bonded to chalcopyrite through hydrogen bonding. This difference was responsible for the excellent depression selectivity of FGG toward monoclinic pyrrhotite flotation and weak depression effect toward chalcopyrite flotation.

Partially hydrolyzed guar gum attenuates non-alcoholic fatty liver disease in mice through the gut-liver axis

BACKGROUND The gut-liver axis has attracted much interest in the context of chronic liver disease pathogenesis.Prebiotics such as dietary fibers were shown to attenuate non-alcoholic fatty liver disease(NAFLD)by modulating gut microbiota.Partially hydrolyzed guar gum(PHGG),a water-soluble dietary fiber,has been reported to alleviate the symptoms of various intestinal diseases and metabolic syndromes.However,its effects on NAFLD remain to be fully elucidated.To determine whether treatment with PHGG attenuates NAFLD development in mice through the gut-liver axis.METHODS Seven-week-old male C57BL/6J mice with increased intestinal permeability were fed a control or atherogenic(Ath)diet(a mouse model of NAFLD)for 8 wk,with or without 5%PHGG.Increased intestinal permeability was induced through chronic intermittent administration of low-dose dextran sulfate sodium.Body weight,liver weight,macroscopic findings in the liver,blood biochemistry[aspartate aminotransferase(AST)and alanine aminotransferase(ALT),total cholesterol,triglyceride,free fatty acids,and glucose levels],liver histology,myeloperoxidase activity in liver tissue,mRNA expression in the liver and intestine,serum endotoxin levels in the portal vein,intestinal permeability,and microbiota and short-chain fatty acid(SCFA)profiles in the cecal samples were investigated.RESULTS Mice with increased intestinal permeability subjected to the Ath diet showed significantly increased serum AST and ALT levels,liver fat accumulation,liver inflammatory(tumor necrosis factor-αand monocyte chemotactic protein-1)and fibrogenic(collagen 1a1 andαsmooth muscle actin)marker levels,and liver myeloperoxidase activity,which were significantly attenuated by PHGG treatment.Furthermore,the Ath diet combined with increased intestinal permeability resulted in elevated portal endotoxin levels and activated toll-like receptor(TLR)4 and TLR9 expression,confirming that intestinal permeability was significantly elevated,as observed by evaluating the lumen-to-blood clearance of fluorescein isothiocyanate-conjugated dextran.PHGG treatment did not affect fatty acid metabolism in the liver.However,it decreased lipopolysaccharide signaling through the gut-liver axis.In addition,it significantly increased the abundance of cecal Bacteroides and Clostridium subcluster XIVa.Treatment with PHGG markedly increased the levels of SCFAs,particularly,butyric acid,acetic acid,propionic acid,and formic acid,in the cecal samples.CONCLUSION PHGG partially prevented NAFLD development in mice through the gut-liver axis by modulating microbiota and downstream SCFA profiles.

Synergistic Interaction and Gelation in Cationic Guar Gum-Sodium Alginate System

The synergistic interaction between the cationic guar gum (the ammonium hydroxy-propyl-trimethyl chloride of guar gum) and sodium alginate has been studied. The effects of the mass ratio of them, mixed temperature, balk salt ion concentration, incubation time and pH value on gelation were investigated. It has been observed that there was a gel strength maximum when the mass ratio was 0.6, the mixed temperature was 70°C, the balk salt ion concentration was 1.0 mol·L?1, the incubation time was 30 min and the pH value was 8. Interaction between molecules of these two polysaccharides was investigated by FT-IR spectrometry. Key words cationic guar gum - sodium alginate - gelation - synergism CLC number O 629.12 Foundation item: Supported by the National Natural Science Foundation of China(29574173)Biography: He Dong-bao (1945-), male, Associate professor, research direction: modifying and gelating of natural polysaccharides.

High molecular weight guar gum assisted settling of fine solids in diluted bitumen:Effect of solvents

As water-based extraction technologies for producing bitumen from oil sands have received increasing environmental concerns,developing non-aqueous extraction(NAE)technique is of both fundamental and practical importance.However,the relatively high concentration of fine solids trapped in the extracted bitumen presents an obstacle for pipeline transport as well as upgrading and refining downstream.This research attempts to provide a solution to fine solids removal without using synthetic additives or affecting bitumen recovery from NAE process.Herein,naturally hydrophilic additives(i.e.,water and high molecular weight guar gum(HGG)produced from Cyamopsiste tragonolobuosr L.Taup.)were introduced to promote the settling of fine solids suspended in bitumen-solvent solution,and the effects of solvents(i.e.,toluene,cyclohexane and their mixtures)were systematically investigated.Aggregate size distribution analyzed by the focused beam reflectance measurements confirmed that the addition of water and HGG could promote the agglomeration and settling of fine solids in all solvents studied.However,the size range and quantity of the agglomerates vary significantly with the solvent's aromatic character.Solvent mixtures demonstrate a superior performance on removing fine solids from bitumen over single solvent.Specifically,in a 3:2 toluene/cyclohexane mixture,the solid content was lowered from 0.66 wt%to 0.09 wt%.

Destabilization of bitumen-coated fine solids in oil through water-assisted flocculation using biomolecules extracted from guar beans

Non-aqueous extraction(NAE)of bitumen from oil sands has been gaining great attention from both the industry and academia as an alternative to the water-based extraction.A fine solids removal step is important for a NAE process in order to obtain high-quality bitumen product,which,however,remains a great challenge to reduce the fine solids content to the desired level.Here,we introduce a strategy of destabilizing the bitumen-coated silica particles in toluene with the addition of water and biomolecules extracted from Cyamopsiste tragonolobuosr L.Taup.,i.e.,high molecular weight guar gum(HGG)and low molecular weight guar gum(LGG),respectively.By virtue of sedimentation tests and focused beam reflectance measurement analysis,we demonstrate that the introduced water droplets modified with these biomolecules can facilitate the settling of the solid particles in toluene although the underlying mechanisms differ between these two biomolecule cases.Specifically,in the case of LGG,the added water droplets with the interfacial amphiphilic LGG can strengthen the attachment of solid particles from bulk toluene to the LGG surface.This research work provides useful insight into the development of effective approaches for destabilization and removal of bitumen-coated fine solids from NAE bitumen.

Effects of Guar (<i>Cyamopsis tetragonoloba</i>) Residues on the Performance and Nutrients Digestibility in Finishing Awassi Lambs

The nutritive quality of guar (Cyamopsis tetragonoloba) residues (GR) was investigated and the effects of partial replacement of wheat straw by GR on nutrients intake, digestibility, and growth performance. Twenty four male lambs, with average body weight (BW) of 40.3 ± 2.5 kg were divided into three groups. Lambs were fed individually for 104 days with concentrate mixture (18% crude protein) and wheat straw as a total mixed ration. Wheat straw was replaced with 0, 100 and 145 g/kg dry matter guar residues. All rations were isonitrogenous and isocaloric. The fattening trial lasted 104 days. At the end of week 10 of the trail, a digestion trial was performed using six lambs from each group. The composition of nutrients in the GR was 87%, 8.5%, 56.4% and 40.1% for DM, CP, NDF and ADF, respectively. DM and crude protein (CP) intake were not affected by the inclusion of GR. However, neutral detergent fibre (NDF) and acid detergent fibre (ADF) intake were lower in lambs fed GR compared to control lambs. At the end of the experiment, lambs fed 100 and 145 g GR/kg DM diets gained more weight (P < 0.05) than those fed the control diet. The same trend was found for feed conversion (CR). Results from this work suggested that GR had advantages compared to regular roughage (wheat straw) in regard to parameters investigated as well as the significant reduction in fattening diets cost.

Effect of water stress at different periods on seed yield and water use efficiency of guar under Shambat conditions

Water stress effects on seed yield and water use efficiency of three indeterminate guar (Cyamopsis tetragonoloba L. Taub.) lines (L12, L18 and L33) were investigated in the experimental farm of the Faculty of Agriculture, University of Khartoum for two seasons (2005 and 2006). The guar lines were subjected to water stress induced by withholding irrigation for three weeks. Three water stress treatments were imposed 35, 50 and 65 days after sowing (DAS), and a control treatment irrigated every two weeks. The treatments were arranged in a split-plot design with three replications;with water regime treatments assigned to the main plots and guar lines to the subplots. Data were recorded on seed yield (t.ha–1), number of pods per plant, 1000- seed weight (g), harvest index (HI) and water use efficiency at harvest. The results indicated that exposure of several cultivars of guar to water stress at the three stages of growth didn’t induce any significant effect on number of pods per plant, 1000-seed weight, seed yield and water use efficiency (WUE). On the other hand there was significant reduction in harvest index as a result of imposition of water stress at 35 and 50 DAS. It was also evident that plants re-watered after the stress recovered and had the same values as the control treatment.

Growth and Yield of Guar (Cyamopsis tetragonoloba L.) Genotypes under Different Planting Dates in the Semi-Arid Southern High Plains

Guar is a drought and salt tolerant summer annual legume, which could be a potential alternative crop in the semi-arid Southern High Plains. Increased use of guar gum in oil industries has increased the demand of guar globally. Planting date effects on stand establishment, physiological parameters, and yield formation of guar genotypes were investigated at the New Mexico State University’s Agricultural Science Center at Clovis, NM for two seasons (2014 and 2015). Four guar genotypes (HES 1123, Kinman, Lewis, and Matador) were tested under three planting dates (June 18, July 7, and July 22 in 2014;and June 18, July 6, and July 20 in 2015). Higher temperature and rainfall were recorded under mid-June planting than early-July and late-July plantings. Guar planted under mid-June had better stand establishment as shown by the higher number of plants m-2, better physiology as revealed by higher photosynthetic rate (Pn), transpiration rate (Tr), leaf area index (LAI), and SPAD values than early-July and late-July plantings. Guar planted under mid-June resulted in taller plants, and therefore, produced higher plant biomass than both of the July plantings. Yield attributing characteristics including clusters plant-1, pods plant-1, seeds plant-1, seed spod-1, 1000 seed weight, and harvest index (HI) were highest under mid-June planting followed by the early-July and late-July plantings, respectively. The mid-June planting increased seed yield by 26% and 55% over early-July and late-July (1399 vs. 1111 and 903 kg·ha-1) plantings, respectively in 2014;while the same increase in 2015 was 51% and 243% (1308 vs. 868 and 381 kg·ha-1), respectively. These results indicate that delaying planting beyond mid-June is detrimental to guar productivity. However, genotypes did not show any significant variation in their performance. Overall, warmer growing conditions and more precipitation under mid-June planting caused better growth and yield formation of guar genotypes.

EFFECT OF ANIONIC SURFACTANT UPON THE VISCOSITY OF POLYMER GUAR GUM SOLUTIONS

The reduced viscosity of polymer guar gum solutions containing a certain concentration of sodium dodecyl benzene sulfonate （SDBS） was measured. It has been found that the Huggins coefficient kH of polymer solutions is very sensitive to the concentration of the surfactant, CSDBS, in solutions. If CSDBS is lower than CMC, the critical micelle concentration of SDBS, kH increases rapidly with CSDBS. On the other hand, if CSDBS is larger than CMC, kH decreases rapidly with CSDBS. Comparatively, the intrinsic viscosity of polymer solution does not show a notable change with CSDBS. The experimental results indicate that the interchain association of polymer guar gum in solution is greatly associated with SDBS interacted with polymer chains through hydrogen bonds. However, the effect of SDBS upon the intrachain association of polymer guar gum solution is negligible, presumably due to the fact that guar gum is a slightly stiffened random-coil chain polymer.

INTERMOLECULAR AND INTRAMOLECULAR INTERACTIONS OF POLYMER GUAR GUM IN SOLUTIONS

The tetrahedral borate ion can crosslink with polymer guar gum in aqueous solutions. If the concentration of guar gum is less than 0.045 g/dL, the intramolecular interaction between guar gum and borate ion increases due to the formation of crosslinks. As a result, the polymer chains of guar gum in solution shrink in size and the reduced viscosity of polymer solution decreases accordingly. On the other hand, if the concentration of guar gum is greater than 0.045 g/dL, the intermolecular interaction becomes apparent due to the same reason. The polymer chains, therefore, associate together and the reduced viscosity of polymer solution increases considerably. According to this technique, the critical concentration c^＊, presented by de-Gennes, is determined successfully.

The Inheritance and Variation of Gum Content in Guar [Cyamopsis tetragonoloba (L.) Taub.]

Guar [Cyamopsis tetragonoloba （L.） Taub.] is one of the most important industrial crops due to its richness in gum. Understanding the inheritance of gum content is a key to its successful genetic improvement. Gum content expression is reported to be controlled by additive, dominant, and epistatic effects, and modified by the environment, fg%, a relative gum content calculated by comparing with the gum content of Kinman was used as gum content （%） in this experiment. Reciprocal crosses of two lines of guar, PI 217923 and Lewis, were made to study the heritability of gum content （fg%）. fg% of four plant introductions and four commercial varieties were studied in Lubbock in 1999-2002. Estimates of broadsense heritability （h^2 b,s.） of fg% in Lewis x PI 217923 and PI 217923 x Lewis were 75.53 and 52.74%, respectively. Estimates of narrow-sense heritability （h2 n.s.） of fg% were 40.00 and 29.00% thr Lewis x PI 217923 and PI 217923 x Lewis, respectively. At least one pair of genes were estimated to control the fg% expression in these two crosses, Significant differences of fg% were found among these eight entries. PI 217923 was found to have the highest fg% among the eight entries.

Initiation of Unmanned Aerial System (UAS) Phenotyping of Plant Height and Canopy Width in Guar

The use of the Unmanned Aerial System (UAS) has attracted scientific attention because of its potential to generate high-throughput phenotyping data. The application of UAS to guar phenotyping remains limited. Guar is multi-purpose legume species. India and Pakistan are the world’s top guar producers. The U.S. is the world guar largest market with an import value of >$1 billion annually. The objective of this study was to test the feasibility of UAS phenotyping of plant height and canopy width in guar. The UAS data were collected from a field plot of 10 guar accessions on July 7, 2021, and September 27, 2021. The study was organized in a Randomized Complete Block Design (RCBD) with 3 blocks. A total of 23 Vegetation Indices (VIs) were computed. The analysis of variance showed significant genotypic effects on plant weight (p < 0.05) and canopy width (p on plant height (p most VIs were significant for both flights (p Vegetation Index (NDVI) and Red Edge Normalized Difference Vegetation Index (NDRE) were significantly and highly correlated with plant height (r = 0.74) and canopy width (r = 0.68). The results will be of interest in developing high throughput phenotyping approach for guar breeding.

Biodegradable Guar Gum Nanoparticles as Carrier for Tamoxifen Citrate in Treatment of Breast Cancer

We prepared, characterized and studied the biodistribution of tamoxifen citrate (TMX) loaded cross-linked guar gum (GG) nanoparticles (NPs). NPs were prepared via a single step emulsion process and particle size evaluated. The extent of tissue distribution and retention following oral administration of TMX loaded GG NPs and TMX tablet in female albino mice was analyzed over a period of 48 hours. Till 48 hours, the particles remained detectable in both mammary and ovary tissue (estrogen receptors). Uptake and retention of TMX from NPs and tablet in mammary gland and ovary tissue changed with time. Results showed that the uptake and retention of NPs was more in the mammary gland between 24 - 48 hours (11.2% at 24 h;4.65% at 48 h). As mammary gland is the target organ in breast cancer therapy, it may be concluded that the cross-linked GG NPs are capable of releasing the drug at the target and minimize the uptake and retention in non target tissue, the ovary (7.98% at 24 h;1.9% at 48 h). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) with time were measured. No abnormal changes in the liver enzymes were observed. GG NPs under study can be used as a drug carrier system for treating cancer.