Numerical Investigations on Harbor Oscillations Induced by Falling Objects

In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.

The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome

Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.

Mathematical Modeling of Moored Ship Motion in Arbitrary Harbor utilizing the Porous Breakwater

The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.

Distribution and ecological evaluation of heavy metals in multi-medium of the Dongzhai Harbor

The distribution characteristics of heavy metals such as Cu, Zn, Pb, Cd, Hg. As in seawater, superficail sediments and several marine organisms of Dongzhai Harbor in Hainan were analyzed. Index methods of the single factor and geo-accumulation were used to assess the pollution degree of heavy metals. The results showed that the heavy metal levels in the seawater of Dongzhai Harbor accorded with the first category of the seawater except Pb, and were much lower than the fishery water quality standard; the concentration of the heavy metal in surface sediments did not exceed the national benchmark of the first-class of marine sediment quality basically, and the superficial sediments were essentially uncontaminated; the heavy metal content in wetland sediments was slightly higher than those in other typical mangrove wetland island of Hainan and marine sediments in Dongzhai Haibor, which indicated this area might has been affected by land-based pollution influence, and this should cause the attention of related departments; the heavy metal concentrations in marine organisms were up to the first category. All were not beyond the standard of non-environmental pollution aquatic products.

Floating-Harbor综合征1例并文献复习

Floating-Harbor综合征(FHS)是由于SRCAP基因突变引起的常染色体显性遗传病。该文报道1例FHS患儿的临床特点。患儿为11岁7个月的男孩,因发现身材矮小8年余就诊。患儿表现为特殊面部特征(三角脸、嘴唇薄、长睫毛)、骨骼畸形(手指弯曲)、语言表达障碍、骨龄落后。基因检测显示SRCAP基因存在新发c.7330C>T(p.R2444X)杂合突变。根据患儿特征性的临床表现及基因检测结果,患儿确诊为FHS。FHS临床罕见,容易漏诊及误诊,基因检测分析有助于FHS患儿的临床诊断。

Source analysis and ecological risk assessment for heavy metals in the sediments of the Yangshan Deepwater Harbor in autumn and winter

Temporal and spatial variations of concentrations of heavy metals including mercury （Hg）, zinc （Zn）, lead （Pb）, arsenic （As）, copper （Cu）, and cadmium （Cd） in the sediments of the Yangshan Deepwater Harbor were determined based on 6 cruises in autumn and winter, respectively, from 2010 to 2013. The results demonstrated that the overall concentrations of heavy metals were low and distributed in uniform patterns. The concentrations of Hg, Zn, Pb, and Cd in autumn were significantly higher than those in winter with small fluctuations for As and Cu in terms of seasonal variations. Results of factor analysis showed that Pb, Cd, and Zn were derived from inland industrial and shipping discharges as well as the degradation of organic pollutants in marine environment. While agricultural pollutions, cargo shifting and construction debris from reclamation projects contributed to the sources of Cu, As, and Hg. Ecological risk assessment by Mean Sediment Quality Guideline Quotient （SQG-Q） revealed that the degree for eco-risk of the sediments was low-and-moderate in autumn, higher than that in winter. Hg and Cu were the dominant eco-risk factors. The results of Index of geoaccumulation （Igeo） showed that the whole sites of the sea area were barely influenced by Hg, As, Zn, and Pb, and the extents of Cd and Cu contaminations were in low grade. Contamination degree of all the six heavy metals could be ranked as the following： Cd〉Pb〉Zn〉Hg〉As. According to the results of integrated score of factor analysis, the contamination degree for heavy metals in sediments of the Yangshan Deepwater Harbor was low, despite sites No.5, No.4 and No.3, which were heavily contaminated compared with others.

基于Harbor实现Docker私有仓库搭建的研究

随着Docker容器技术的广泛应用,基于安全角度考虑,越来越多的企业需要搭建自己的Docker私有仓库,以方便对私有镜像的管理。Docker私有仓库的搭建可采用Registry和Harbor两种方式。在对比两种搭建方式的基础上,主要对Harbor的整体架构和实现过程进行介绍,并应用案例加以实现。

基于Harbor搭建高可用Docker私有仓库的研究与实现

随着容器技术的广泛应用,越来越多的大中型企业在生产环境中通过Harbor部署Docker私有仓库。从高可用角度考虑,如何解决在生产环境中因出现单点故障从而对项目开发造成影响并造成经济损失的问题变得尤为重要。该文通过对比三种Harbor高可用性方案后,对基于共享服务的Harbor同步方案进行实现。

Effect of Development of Caofeidian Harbor Area in Bohai Bay on Hydrodynamic Sediment Environment

Based on the characteristics of waves, tidal currents, sediment and seabed evolution in the Caofeidian sea area in the Bohai Bay, a 2D sediment mathematical model of waves and tidal currents is employed to study the development schemes of the harbor. Verification of spring and neap tidal currents and sediment in the winter and summer of 2006 shows that the calculated values of tidal stages as well as flow velocities, flow directions and sediment concentration of 15 synchronous vertical lines are in good agreement with the measured data. Also, deposition and erosion of the sea area in front of Caofeidian ore terminal induced by suspended load under tidal currents and waves are verified; it shows that the calculated values of depth of deposition and erosion as well as their distribution are close to the measured data. Furthermore, effects of reclamation scheme of island in front of the land behind Caofeidian harbor on the hydrodynamic environment are studied, including changes of flow velocities in the deep channels at the south side of Caofeidian foreland and Laolonggou and in various harbor basins, as well as changes of deposition and erosion of seabed induced by the project.

Theoretical Analysis of Harbor Resonance in Harbor with An Exponential Bottom Profile

The general features of oscillations within a rectangular harbor of exponential bottom are investigated analytically. Based on the linear shallow water approximation, analytical solutions for longitudinal oscillations induced by the incident perpendicular wave are obtained by the method of matched asymptotics. The analytic results show that the resonant frequencies are shifted to larger values as the water depth increases and the oscillation amplitudes are enhanced due to the shoaling effect. Owing to the refraction effect, there could be several transverse oscillation modes existing in when the width of the harbor is on the order of the oscillation wavelength. These transverse oscillations are similar to standing edge waves, and there are m node lines in the longshore direction and n node lines running in the offshore direction corresponding to mode（n, m）. Furthermore, the transverse eigen frequency is not only related to the width of the harbor, but also to the boundary condition at the backwall and the bottom shape.

Long-term temporal-spatial dynamics of marine coastal water quality in the Tolo Harbor,Hong Kong,China

The long-term temporal and spatial dynamics of marine coastal water quality in Tolo Harbor, Hong Kong were explored. The Harbor is divided into three zones represented as Harbor, Buffer, and Channel Subzones. The time range for the study covers the period from the 1970s to the 1990s. The selected indicators for the comprehensive assessment of water quality consist of physical, chemical and biological aspects, including suspended solids(SS), Secchi disk depth(SD), 5-day biochemical oxygen demand(BOD\-5), total nitrogen(TN), total phosphorus(TP), faecal coliform, chlorophyll-a(Chl-a), and the number of red tide occurrences. The results indicated the presence of obvious temporal and spatial trends with regard to changes in water quality. Spatially, water quality in the Channel Subzone is the best, while that in the Harbor Subzone is the worst. On a temporal basis, the average trend from bad to good was 1980s>1990s>1970s as indicated by most of the selected water quality indicators. Water quality during the late 1980s reached its worst level with the lowest SD, the highest BOD\-5, TN, TP, Chl-a concentrations, and the number of red tide occurrences. These long-term temporal-spatial water quality trends were also found in other studies of the Tolo Harbor. The large quantity of pollutants produced as a result of increasing population, industrial and commercial actives, and urbanization and industrialization trends in both Shatin and Tai Po seem to be primarily responsible for the changes in marine coastal water quality.

A composite numerical model for wave diffraction in a harbor with varying water depth

A composite numerical model is presented for computing the wave field in a harbor. The mild slope equation is discretized by a finite element method in the domain concerned. Out of the computational domain, the water depth is assumed to be constant. The boundary element method is applied to the outer boundary for dealing with the infinite boundary condition. Because the model satisfies strictly the infinite boundary condition, more accurate results can be obtained. The model is firstly applied to compute the wave diffraction in a narrow rectangular bay and the wave diffraction from a porous cylinder. The numerical results are compared with the analytical solution, experimental data and other numerical results. Good agreements are obtained. Then the model is applied to computing the wave diffraction in a square harbor with varying water depth. The effects of the water depth in the harbor and the incoming wave direction on the wave height distribution are discussed.

A new electroplated Ir/Ir(OH)_x pH electrode and its application in the coastal areas of Newport Harbor, California

Resulting from the rising levels of atmospheric carbon, ocean acidification has become a global problem. It has significant impacts on the development, survival, growth and physiology of marine organisms. Therefore, a high- precision sensor is urgently needed to measure the pH of sea-water. Iridium wire with a diameter of 0.25 mm is used as the substrate, and an Ir/Ir（OH）x pH electrode is prepared by a one-step electrochemical method in a LiOH solution at the room temperature. A scanning electron microscope （SEM） observation reveals that it is coated with nanoscale particles. In laboratory tests, the electrode exhibits a very promising pH response, with an ideal Nernst slope （56.14-59.52）, fast response, good stability and long fife-span in tested pH buffer solutions. For a sea trial, four pH electrodes and one Ag/AgC1 reference electrode are integrated with a self-made chemical sensor, and a profile detection of nearly 70 m is implemented near Newport Harbor, California on August 3, 2015. The results reflect that the pH value measured by the sensor is very close to the data given by Sea-Bird 911 plus CTD, with a difference value ranging from 0.000 075 to 0.064 719. And the sensor shows a better data matching degree in 0-40 m water depth. In addition, the high precision and accuracy of the sensor make it possible to use in the ocean observation field.

Similarities between Yangshan Harbor area and the Yangtze estuary

By analysis of published papers on the Yangtze estuary and hydrological and sediments data in Yangshan Harbor area, many similarities are found between Yangshan Harbor area and the Yangtze estuary. These similarities include the phenomenon of stagnating flow areas, the distributive characteristics of the highest suspended sediment concentration areas, superficial sediments and shoal bars. The stagnating flow area is the major similarity which causes other similarities. These similarities indicate that: 1) Turbidity Maximum and mouth bars in estuaries are mainly caused by the hydraulic balance of stagnating flow areas of estuaries; 2) The stagnating sand area of sands caused by stagnating flow area often locates on the narrower side of the stagnating flow area; 3) The location (or shape) of fine sediments area caused by stagnating flow area reflects the location (or shape) of the stagnating flow area. Both Yangshan Harbor area and the Yangtze estuary are the important developmental areas in the future (man-made similarity). In-depth studies on these similarities between Yangshan Harbor area and the Yangtze estuary will have momentous theoretical and practical significance.

Study on Influences of Fringing Reef on Harbor Oscillations Triggered by N-Waves

Influences of topographic variations of the offshore fringing reef on the harbor oscillations excited by incident Nwaves with different amplitudes and waveform types are studied for the first time.Both the propagation of the Nwaves over the reef and the subsequently-induced harbor oscillations are simulated by a Boussinesq-type numerical model,FUNWAVE-TVD.The present study concentrates on revealing the influences of the plane reef-face slope,the reef-face profile shape and the lagoon width on the maximum runup,the wave energy distribution and the total wave energy within the harbor.It shows that both the wave energy distribution uniformity and the total wave energy gradually increase with decreasing reef-face slope.The profile shape of the reef face suffering leading-elevation Nwaves(LEN waves)has a negligible impact on the wave energy distribution uniformity,while for leading-depression N-waves(LDN waves),the latter gradually decreases with the mean water depth over the reef face.The total wave energy always first increases and then decreases with the mean water depth over the reef face.In general,the total wave energy first sharply decreases and then slightly increases with the lagoon width,regardless of the reef-face width and the incident waveform type.The maximum runup subjected to the LEN waves decreases monotonously with the lagoon width.However,for the LDN waves,its changing trend with the lagoon width relies on the incident wave amplitude.

Effects of methyl-,phenyl-,ethylmercury and mercurychlorid on immune cells of harbor seals(Phoca vitulina)

Mercury （Hg） is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the influence of methyl-, ethyl- and phenylmercury as well as mercurychloride on immune functions was evaluated. Two parameters of cellular immunity, proliferation and mRNA cytokine expression of interleukin-2, -4, and transforming growth factor β, were investigated in harbor seal lymphocytes after in vitro exposure to Hg compounds. While all Hg compounds had a suppressive effect on proliferation, differences between juvenile and adult seals were found. Lymphocytes from juveniles showed a higher susceptibility to the toxic effect compared to lymphocytes from adults. Furthermore, the degree of inhibition of proliferation varied among the four Hg compounds. The organic compounds seem to be more immunotoxic than the inorganic compound. Finally, for the cytokine expression of methylmercury-incubated lymphocytes, timedependent changes were observed, but no dose-dependency was found. Marine mammals of the North Sea are burdened with Hg, and lymphocytes of harbor seals may be functionally impaired by this metal. The present in vitro study provides baseline information for future studies on the immunotoxic effects of Hg on cellular immunity of harbor seals.

Ecological features of phytoplankton in red tide outburst area in the Xiamen Harbor

The results from the 38 cruises of phytoplankton surveys in the red tide outburst area in the XiamenHarbor in 1987 indicate 41 species of red tide organisms among 110 species of phytoplankton identified. Species composition was dominant by eurytopic species and warm water species. Species diversity apparently changed with (coldand warm) seasons. There was a high diversity in warm seasons, 63 species in August. Average annual number ofphytoplankton reached 1 119 × 103 ind. /dm3 which was 2 to 2. 6 times that in 2 control monitoring stations. Thenumber of phytoplankton in horizonal distribution displayed an obvious increasing tendency from the harbour mouthto the inner harbor. The phytoplankton number always changed with time, but with 3 peak values in the year round.The daily change of the phytoplankton number with tide exhibited a negative relativity. Moreover phytoplanktonspotes and their number showed a vertical movement during day and night. Futhermore, 10 predominant specieschanged with seasons was noted in the present paper.

Change of Back Siltation in Lianyungang Harbor before and after Completion of the West Breakwater

On the basis of a comparison of the field hydrological, sediment and topographic data observed before and after the completion of the West Breakwater in Lianyungang Harbor, which has led to the change of topography from a strait to a bay, a synthetical analysis is conducted on the change of back siltation. The results show that it is the change of coast line and the consequent change of hydrological condition and sediment movement that has led to the change of back siltation in the harbor. This phenomenon is caused by the lag effects of water current, sediment movement and local adjustment of topography after the construction, and it tends to be stable. The results of analysis provide not only a basis for extension of Lianyungang Harbor into a deep water harbor, but also propose a case for the study of the change of back siltation in a harbor on muddy coast.

Distribution and Sources of Polycyclic Aromatic Hydrocarbons (PAHs) in the Surface Seawater of the Western Xiamen Harbor

The concentration of PAHs in the surface seawater of the western Xiamen Harbor has been analysed in the paper. The PAHs concnetration at 9 station locations varies greatly within the range of 106.1ng/L ~ 4365ng/L. But PAHs pollution is slight as compared with that in similar areas in other parts of the world. PAHs stem mainly from oil pollution, urban and industrial discharges, and combustion of fuels.

Ecological risk assessment of surficial sediment by heavy metals from a submerged archaeology harbor,South Mediterranean Sea,Egypt

A protected semi-enclosed embayment,the Eastern Harbor(EH),is situated in the central sector of the Mediterranean coast of Alexandria,Egypt.It is a famous fishing dock and water-sport center and its importance arose after the discovery of the Ptolemaic royal quarter of ancient Alexandria.To protect this national treasure and improve its environmental health,the untreated anthropogenic effluents,which continued for decades into the EH since 1992,must be totally stopped.Accordingly,it is an important issue to continually monitor and asses the environmental status of the EH.The sediment texture,total organic carbon,and the accumulation,distribution,and ecological risks of Cd,Cr,Pb,As,Hg and A1 were determined with a total of 14 surficial sediments samples collected in the EH during 2010.Metal contents were compared to the literature data to assess the pollution status of sediments.Enrichment factors,Degree of contamination(mCd)and potential ecological risk were calculated as a criterion of possible contamination.Since mCd calculation produces an overall average value for a range of pollutants,in the present study the contamination factor that provides a measure of the degree of overall contamination in the surface layers in a particular sampling site may provide a better indication of the state of pollution in the EH sediments than other indicators.