期刊文献+
共找到394篇文章
< 1 2 20 >
每页显示 20 50 100
Expression of Heat Shock Protein 70 and 27 in Non-small Cell Lung Cancer and Its Clinical Significance 被引量:5
1
作者 黄畦 祖育昆 +1 位作者 付向宁 邬堂春 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第6期693-695,共3页
The heat shock proteins (HSPs) 70 and HSP 27 expression in patients with non-small cell lung cancer (NSCLC) was studied and the relation.ship between HSP 70 and HSP 27 with the clinicopathological features of NSCL... The heat shock proteins (HSPs) 70 and HSP 27 expression in patients with non-small cell lung cancer (NSCLC) was studied and the relation.ship between HSP 70 and HSP 27 with the clinicopathological features of NSCLC was investigated. The expression of HSP 70 and HSP 27 was detected in tumor tissues from 60 patients with NSCLC by S-P immunohistochemistry. The findings were analyzed in combination with the histological types, histopathological differentiation, lymph node metastasis, patients' clinical stages, smoking history and gender. The results showed that of the 60 NSCLC tissue specimens studied, the immunoreactivity of HSP 70 and HSP 27 was detected in 47 (78.3 %) and 43 (71.7 %) specimens, respectively. A positive correlation was found between the overexpression of HSP 70 and HSP 27. The histopathological differentiation, lymph node metastasis, clinical stages and smoking history were correlated to the expression of HSP 70, but not to the expression of HSP 27. No statistical significance was observed in histological types and gender with respect to both HSP 70 and HSP 27 expression. It is suggested that the HSP 70 expression is a powerful and significant prognostic indicator and is related to histopathological differentiation, lymph node metastasis, patients' clinical stages, smoking history, whereas HSP 27 expression is not. 展开更多
关键词 non-small cell lung cancer heat shock protein 70 and 27 IMMUNOHISTOCHEMISTRY
下载PDF
Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2 被引量:3
2
作者 Li Liu Xiaojin Zhang +3 位作者 Surong Jiang Xiang Gao Guoxain Ding Yunlin Cheng 《Journal of Nanjing Medical University》 2005年第4期187-190,共4页
Objective: To clone human cardiac heat shock protein 27 (HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got ... Objective: To clone human cardiac heat shock protein 27 (HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got by RT-PCR was constructed into pCDNA3.1^+ . The recombinant was transfected into rat cardiomyocyte cell line H9c2 and the stable trahsfection cell line was selected by G418. Then we observe the effects of HSP27 over-expression on LDH release and apoptosis induced H2O2 in H9c2. Results: ①pCDNA3.1^+/HSP27 provided a sound expression of HSP27 in both 293T and H9c2. ②LDH releasing induced by 0, 100,250,500, 1000 μmol/L H2O2 in HSP27 over-expression group and wild type group were 0.396±0.017 vs. 0.390±0.01)9 (p 〉0.05), 0.437±0. 014 vs. 0.416±0.015 (P〈0.05), 0.471±0.018 vs. 0.417±0.009 (P 〈0.001), 0.505±0.030 vs. 0.657± 0.022(P 〈0.001), 0.547 ±0.027 and 0.661 ± 0.011( P 〈 0. 001 ), respectively. ③Apoptosis induced by 150 μmol/L H2O2 in HSP27 over-expression group and wild type group were (10.693± 1.122)% vs. (4.027 ± 1.628)%( P 〈0.01). Conclusion: We cloned and constructed human cardiac HSP27 gene successfully, and over-expression of human HSP27 could inhibit oxidative damage significantly in H9c2. 展开更多
关键词 heat shock protein 27 Molecular Cloning TRANSFECTION Oxidafive Stress APOPTOSIS
下载PDF
Malarial pigment enhances heat shock protein-27 in THP-1 cells:new perspectives for in vitro studies on monocyte apoptosis prevention 被引量:1
3
作者 Mauro Prato Valentina Gallo +3 位作者 Elena Valente Amina Khadjavi Giorgia Mandili Giuliana Giribaldi 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第12期934-938,共5页
Objective:To investigate the effect of malarial pigment(hemozoin,HZ) on expression of heat shock proteins(HSPs) and cell viability in human monocytes by using a stable cell line(THP-1 cells).Methods:THP-1 cells were f... Objective:To investigate the effect of malarial pigment(hemozoin,HZ) on expression of heat shock proteins(HSPs) and cell viability in human monocytes by using a stable cell line(THP-1 cells).Methods:THP-1 cells were fed with native HZ or treated with pro-apoptotic molecule gliotoxin for 9 h.Thereafter,the protein expression of HSP-27 and HSP-70 was evaluated by western blotting.Alternatively,HZ-fed cells were cultured up to 72 h and cell viability parameters(survival,apoptosis and necrosis rates) were measured by flow cytometric analysis. Results:HZ increased basal protein levels of HSP-27 without altering those of HSP-70 in THP-1 cells,and promoted long-term cell survival without inducing apoptosis.As expected,gliotoxin inhibited HSP-27 protein expression and promoted long-term cell apoptosis.Conclusions: Present data show that HZ prevents cell apoptosis and enhances the expression of anli-apoptotic HSP-27 in THP-1 cells,confirming the previous evidences obtained from HZ-fed immunopurified monocytes.Since the use of a stable cell line is pivotal to perform HSP-27 silencing experiments, monocytic THP-1 cells could be a good candidate line for such an approach,which is heavily required to clarify the role of HSP-27 in survival of impaired HZ-fed monocytes during falciparum malaria. 展开更多
关键词 HEMOZOIN PLASMODIUM FALCIPARUM Malaria MONOCYTE THP-1 cells Phagocytosis APOPTOSIS heat shock protein-27
下载PDF
Sensitized heat shock protein 27 induces retinal ganglion cells apoptosis in rat glaucoma model 被引量:1
4
作者 Wei Zhao Le Dai +3 位作者 Xiao-Ting Xi Qian-Bo Chen Mei-Xia An Yan Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2020年第4期525-534,共10页
AIM: To investigate the relationships between the changes of heat shock protein 27 antibody(anti-HSP27) in serum/cerebrospinal fluid(CSF), intraocular pressure(IOP), retinal ganglion cell(RGC) apoptosis in a rat glauc... AIM: To investigate the relationships between the changes of heat shock protein 27 antibody(anti-HSP27) in serum/cerebrospinal fluid(CSF), intraocular pressure(IOP), retinal ganglion cell(RGC) apoptosis in a rat glaucoma model and disclose the underlying pathogenesis of glaucoma.METHODS: A total of 115 Wistar rats were randomly divided into 4 groups. Group 1 was the ocular hypertension group by condensing 3 episcleral & limbal veins or episcleral area of right eye(HP group, n=25) and sham operation group with conjunctiva incision without coagulation(n=25). Group 2: HSP27 or dose-matched PBS was injected into the vitreous(V-HSP27 group, n=15;V-PBS group, n=15). Group 3: HSP27 and complete Freund's adjuvant or dosematched PBS was injected subcutaneously into the hind limb accompanied intraperitoneal injection of pertussis toxin [sensitized group(I-HSP27 group), n=15;I-PBS group, n=15)]. Group 4 was normal group without any treatment(n=5). IOPs of the rats were measured before, day 3, weeks 1, 2, 4, 6, and 8 after treatment. Paraffin-embedded sections were prepared for HE staining and RGCs apoptosis were detected by TUNEL. Anti-HSP27 level in serum and CSF were examined by ELISA. RESULTS: IOPs were elevated significantly in HP and V-HSP27, V-PBS groups(P<0.01) and positively related to anti-HSP27 levels in serum and CSFs. Anti-HSP27 levels in serum and CSF were elevated significantly in I-HSP27group compared to other groups(P<0.05). However, the IOPs did not show any relationship with the high-level antiHSP27 in serum and CSFs. RGC apoptosis were all elevated significantly in the HP, V-HSP27, V-PBS and I-HSP27 groups and also positively relative with anti-HSP27 level in serum and CSFs except that high-level of anti-HSP27 in the serum of I-HSP group.CONCLUSION: The increases of anti-HSP27 levels in serum and CSFs both promote IOP escalation and the increase of RGC apoptosis in retina when anti-HSP27 is at low level. The case of high-level anti-HSP27 is opposite and shows protective function in preventing IOP increase and RGC apoptosis. 展开更多
关键词 INTRAOCULAR pressure heat shock protein 27 RETINAL GANGLION cells AUTOIMMUNE APOPTOSIS GLAUCOMA rats
下载PDF
血清HMGB1、CCL20、HSP27水平与慢性牙周炎患者牙周病变程度的相关性分析 被引量:1
5
作者 王伟新 张丽娜 《河南医学研究》 CAS 2024年第2期252-255,共4页
目的探讨血清高迁移率族蛋白1(HMGB1)、CC趋化因子配体20(CCL20)、热休克蛋白27(HSP27)水平与慢性牙周炎(CP)患者牙周病变程度的相关性。方法选取2021年9月至2023年8月在新乡医学院第一附属医院诊治的60例CP患者纳入观察组,根据1∶1原则... 目的探讨血清高迁移率族蛋白1(HMGB1)、CC趋化因子配体20(CCL20)、热休克蛋白27(HSP27)水平与慢性牙周炎(CP)患者牙周病变程度的相关性。方法选取2021年9月至2023年8月在新乡医学院第一附属医院诊治的60例CP患者纳入观察组,根据1∶1原则,另选取同期牙周健康者60例纳入对照组。比较两组及不同牙周病变程度CP患者血清HMGB1、CCL20、HSP27水平,分析各指标水平与CP牙周病变程度的相关性及联合诊断价值,并分析不同血清水平患者发生CP的危险度。结果观察组血清HMGB1、CCL20、HSP27水平高于对照组(P<0.05);不同牙周病变程度CP患者血清HMGB1、CCL20、HSP27水平比较:轻度<中度<重度,且各指标水平与牙周病变程度均呈正相关(P<0.05);入院时HMGB1、CCL20、HSP27水平联合诊断CP的曲线下面积(AUC)为0.905,最佳诊断敏感度为91.67%,特异度为88.33%,约登指数0.800,且各指标高水平患者发生CP的危险度是低水平的1.105倍、1.034倍、1.105倍(P<0.05)。结论HMGB1、CCL20、HSP27在CP患者血清中呈异常高表达,各指标水平与牙周病变程度均呈正相关,且联合检测对CP具有较高诊断价值,可作为临床诊断CP、评估牙周病变程度的有效指标。 展开更多
关键词 高迁移率族蛋白1 CC趋化因子配体20 热休克蛋白27 慢性牙周炎
下载PDF
壳聚糖纳米颗粒负载落新妇苷通过调控MAPK14/HSP27影响高糖诱导的肾小管上皮细胞铁死亡
6
作者 陈毅君 李湘 胡剑卓 《安徽医科大学学报》 CAS 北大核心 2024年第9期1610-1620,共11页
目的探讨壳聚糖纳米颗粒(CS NPs)负载落新妇苷(落新妇苷-CS-NPs)通过调控丝裂原活化蛋白激酶14(MAPK14)/热休克蛋白27(HSP27)对高糖(HG)诱导的肾小管上皮细胞铁死亡的影响。方法将HK-2细胞分组如下:正常糖(NG)组、HG组、HG+落新妇苷-CS-... 目的探讨壳聚糖纳米颗粒(CS NPs)负载落新妇苷(落新妇苷-CS-NPs)通过调控丝裂原活化蛋白激酶14(MAPK14)/热休克蛋白27(HSP27)对高糖(HG)诱导的肾小管上皮细胞铁死亡的影响。方法将HK-2细胞分组如下:正常糖(NG)组、HG组、HG+落新妇苷-CS-NPs(0、5、10、20 mg/L)组,以及HG条件下的pcDNA3.1-MAPK14组、pcDNA3.1-MAPK14+落新妇苷-CS-NP组、si-HSP27组、pcDNA3.1-MAPK14+si-HSP27组、pcDNA3.1-MAPK14+si-HSP27+落新妇苷-CS-NPs组。CCK-8法检测细胞活力,TUNEL法检测细胞凋亡。同时通过试剂盒测定铁离子水平、乳酸脱氢酶(LDH)活性、谷胱甘肽(GSH)水平、活性氧(ROS)水平。构建糖尿病肾脏病(DKD)大鼠模型,并使用落新妇苷-CS-NPs干预,探究其对体内DKD的影响。结果与NG组相比,HG组HK-2细胞活力降低、凋亡增加,铁离子水平、LDH活性、ROS水平升高,而GSH水平明显降低(P<0.05)。经过落新妇苷-CS-NPs处理组明显逆转了HG对HK-2细胞生物学行为及铁死亡相关指标的影响。此外,相比于pcDNA3.1组,pcDNA3.1-MAPK14组铁死亡增加,而敲减HSP27或者联合落新妇苷-CS-NPs干预则改善了这一情况。体内实验结果表明,落新妇苷-CS-NPs能够改善DKD大鼠肾损伤、抑制铁离子水平以及MAPK14/HSP27的表达。结论落新妇苷-CS-NPs可能通过抑制MAPK14与HSP27表达,改善HG诱导的肾小管上皮细胞铁死亡。 展开更多
关键词 落新妇苷 壳聚糖纳米颗粒 丝裂原活化蛋白激酶14 热休克蛋白27 铁死亡 糖尿病肾病
下载PDF
Abrogation of heat-shock protein (HSP)70 expression induced cell growth inhibition and apoptosis in human androgen-independent prostate cancer cell line PC-3m 被引量:7
7
作者 Zhi-GangZhao Qing-ZhengMa Chun-XiaoXu 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第4期319-324,共6页
Aim: To investigate the effect of abrogating heat shock protein (HSP) 70 expression by antisense HSP70 oligonucleotides treatment on human androgen-independent prostate cancer cell line PC-3m growth. Methods: PC-3m ce... Aim: To investigate the effect of abrogating heat shock protein (HSP) 70 expression by antisense HSP70 oligonucleotides treatment on human androgen-independent prostate cancer cell line PC-3m growth. Methods: PC-3m cells were treated with 0-16 μmol/L antisense HSP70 oligomers for 0-100 hr. Cell growth inhibition was analyzed using a trypan blue dye exclusion test. Apoptotic cells were detected and confirmed by flow cytometric analysis and DNA fragmentation analysis. The protein expression of HSP70 and bcl-2 affected by antisense HSP70 oligomers were determined using Western blot. Results: Antisense HSP70 oligomer induced apoptosis and then inhibited proliferation of PC-3m cells in a dose- and time-dependent manner. Ladder-like patterns of DNA fragments were observed in PC-3m cells treated with 10 μmol/L antisense HSP70 oligomer for 48 hr or 8 μmol/L for 72 hr on agarose gel electrophoresis. Antisense HSP70 oligomer pretreatment enhanced the subsequent induction of apoptosis by heat shock in PC-3m cells. In addition, undetectable HSP70 expression was observed at a concentration of 10 μmol/L antisense HSP70 oligomer treatment for 48 hr or 8 μmol/L for 72 hr in Western blot, which was paralleled by decreased expression levels of anti-apoptotic protein bcl-2. Conclusion: HSP70 antisense oligomer treatment abrogates the expression of HSP70, which may disrupt HSP70-bcl-2-interactions and further down-regulate bcl-2 expression, in turn inducing apoptosis and inhibiting cell growth in PC-3m cells. 展开更多
关键词 prostate carcinoma heat shock protein (HSP) bcl-2 protein APOPTOSIS PROLIFERATION
下载PDF
cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China 被引量:6
8
作者 YU Hao WAN Fang-hao GUO Jian-ying 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期293-302,共10页
Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects,and heat shock proteins(Hsps)play important roles in the temperature adaptation of various organisms.To better underst... Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects,and heat shock proteins(Hsps)play important roles in the temperature adaptation of various organisms.To better understand the temperature tolerance of the indigenous ZHJ2-biotype of whitefly Bemisia tabaci species complex,we obtained complete cDNA sequences for hsp90,hsp70,and hsp20 and analyzed their expression profiles under different high temperature treatments by real-time quantitative polymerase chain reaction.The high temperature tolerance of B.tabaci ZHJ2-biotype was determined by survival rate after exposure to different high temperatures for 1 h.The results showed that after 41°C heat-shock treatment for 1 h,the survival rates of ZHJ2 adults declined significantly and the estimated temperature required to cause 50% mortality(LT50)is 42.85°C for 1 h.Temperatures for onset(Ton)or maximal(Tmax)induction of hsps expression in B.tabaci ZHJ2-biotype were 35 and 39°C(or 41°C).Compared with previous studies,indigenous ZHJ2-biotype exhibits lower heat temperature stress tolerance and Ton(or Tmax)than the invasive B-biotype. 展开更多
关键词 Bemisia tabaci biological invasion THERMOTOLERANCE indigenous and invasive biotypes heat shock protein (hsp)
下载PDF
Effects of Sodium Salicylate on the Expression of HSP27 Protein during Oxidative Stress in Tissue-cultured Human Lens Epithelial Cells 被引量:5
9
作者 王智 周莉 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第6期753-755,共3页
The effects of sodium salicylate on the expression of heat shock protein 27 (HSP27) during oxidative stress in tissue-cultured human lens epithelial cells were investigated. Cultured human lens epithelial cells (HL... The effects of sodium salicylate on the expression of heat shock protein 27 (HSP27) during oxidative stress in tissue-cultured human lens epithelial cells were investigated. Cultured human lens epithelial cells (HLB-3) were divided into 3 groups: control group (group A), oxidation injury group (group B) and sodium salicylate group (group C). Apoptosis of human lens epithelial cells cultured in vitro was induced in the presence of 150 μmol/L H2O2. Cells viability and the expression of HSP27 were analyzed. Viability of the cells was measured by methyl thiazole tetrazolium (MTT) chromatometry. The expression of HSP27 in HLB-3 cells was detected by using immunohistochemistry and image analysis system, Sodium salicylate could induce the expression of HSP27, and the cells viability in group C was significantly higher than in group B (0.2667±0.01414 vs 0.2150±0.01080, P=0.012〈0.05). The average gray value of HSP27 in group B was less than that in group C (P=0.000〈0.05). The increased expression of HSP27 by sodium salicylate might play an important role in the protection of hydrogen peroxide-induced injury of human lens epithelial cells, suggesting that sodium salicylate could suppress, at least in part, the apoptosis of human lens epithelial cells. 展开更多
关键词 APOPTOSIS human lens epithelial cells heat shock protein 27 sodium salicylate hydrogen peroxide
下载PDF
What we know about ST13, a co-factor of heat shock protein, or a tumor suppressor? 被引量:6
10
作者 SHI Zheng-zheng, ZHANG Jia-wei, ZHENG Shu (The Second Affiliated Hospital, Cancer Institute, School of Medicine, Zhejiang University, Hangzhou 310009, China) 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第3期170-176,共7页
This article is to summarize the molecular and functional analysis of the gene “suppression of tumorigenicity 13” (ST13). ST13 is in fact the gene encoding Hsp70 interacting protein (Hip), a co-factor (co-chaperone)... This article is to summarize the molecular and functional analysis of the gene “suppression of tumorigenicity 13” (ST13). ST13 is in fact the gene encoding Hsp70 interacting protein (Hip), a co-factor (co-chaperone) of the 70-kDa heat shock proteins (Hsc/Hsp70). By collaborating with other positive co-factors such as Hsp40 and the Hsp70-Hsp90 organizing protein (Hop), or competing with negative co-factors such as Bcl2-associated athanogen 1 (Bag1), Hip facilitates may facilitate the chaperone function of Hsc/Hsp70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and regulators of proliferation or apoptosis. Although the nomenclature of ST13 implies a role in the suppression of tumorigenicity (ST), to date available experimental data are not sufficient to support its role in cancer development, except for the possible down-regulation of ST13 in gastric and colorectal cancers. Further investigation of this gene at the physiological level would benefit our understanding of diseases such as endocrinological disorders, cancer, and neurodegeneration commonly associated with protein misfolding. 展开更多
关键词 ST13 Hip p48 Hsc/Hsp70 heat shock protein protein folding STEROID receptor Tumorigenicity
下载PDF
Heat shock protein inhibitor, quercetin, as a novel adjuvant agent to improve radiofrequency ablation-induced tumor destruction and its molecular mechanism 被引量:4
11
作者 Wei Yang Ming Cui +5 位作者 Jungchieh Lee Wei Gong Song Wang Jingjing Fu Gongxiong Wu Kun Yan 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2016年第1期19-28,共10页
Background: We investigated the effect of a small molecular inhibitor of heat shock protein (HSP), qnercetin, on tumor radiofrequency (RF) ablation, and explored the underlying molecular mechanisms. Methods: In ... Background: We investigated the effect of a small molecular inhibitor of heat shock protein (HSP), qnercetin, on tumor radiofrequency (RF) ablation, and explored the underlying molecular mechanisms. Methods: In in vivo study, rats with R3230 breast adenocarcinoma were sacrificed 24 h post-treatment and gross coagulation areas were compared, and next, randomized into four treatment arms (control, quercetin alone, RF alone, and combination) for Kaplan-Meier analysis of defined endpoint survival. Then the distribution and expression levels of heat shock protein 70 (HSP70), cleaved caspase-3 and heat shock factor 1 (HSF1) were analyzed after different treatments. In in vitro study, we used quercetin to promote SK- HEP-I (hepatic) and MCF-7 (breast) cancer cell apoptosis in heat shock cell model, and siRNA was used to block c-Jun and to explore the role of activating protein-1 (AP-1) signaling pathways. Results: We found the effects of quercetin plus RFA resulted in increase on the tumor destruction/ endpoint survival (26.5±3.4 d) in vivo, compared with RF alone (17.6±2.5 d) and quercetin alone (15.7±3.1 d). Most importantly, quercetin-induced cancer cell death required the presence of HSF1 in animal model. Furthermore, quercetin directly down-regulated expression of HSF1 in vitro, which our findings have revealed, required the activation of AP-1 signaling pathways by loss-of-function analysis using siRNA mediated targeting of c-Jun. Conclusions: These results indicated a protective role of quercetin in tumor ablation and highlighted a novel mechanism involving HSP70 with HSF1 pathway in thermal ablation of solid tumors. 展开更多
关键词 Radiofrequency ablation (RFA) heat shock protein (HSP) QUERCETIN solid tumor
下载PDF
Advance in Research on Biological Function and TranscriptionalControl of Heat Shock Proteins 被引量:1
12
作者 徐存拴 辛泽华 袁金云 《Developmental and Reproductive Biology》 2002年第2期130-136,共7页
The regulation of heat shock transcription factor to heat shock protein expression and the newest knowledge about the effect of heat shock protein on aging,immune response and the balance of cell survival and apoptosi... The regulation of heat shock transcription factor to heat shock protein expression and the newest knowledge about the effect of heat shock protein on aging,immune response and the balance of cell survival and apoptosis are summarized in the paper. 展开更多
关键词 heat shock protein (HSP) heat shock transcription factor (HSF) aging immune response the balance of cell survival and apoptosis
下载PDF
STUDY ON THE ANTI-TUMOR EFFICACY INDUCED BY HEAT SHOCK PROTEIN 70-PEPTIDE COMPLEXES DERIVED FROM TUMOR CELLS 被引量:6
13
作者 傅庆国 张玮 +2 位作者 孟凡东 郭仁宣 姚振宇 《Chinese Medical Sciences Journal》 CAS CSCD 2002年第3期153-156,共4页
OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric an... OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used. RESULTS: HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P 展开更多
关键词 NEOPLASM heat shock protein 70 peptide complexes tumor vaccineObjective. To study the efficacy and explore the mechanism of the anti tumor immunity elicited by heat shock protein 70 peptide complexes (HSP70 PC) derived from tumor cells. M
下载PDF
Detection and Its Implication of Heat Stress Protein 27 and P21 in Nasopharyngeal Carcinoma 被引量:1
14
作者 刘立思 肖成峰 陶雁玲 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第4期336-337,342,共3页
To study the expression of heat stress protein 27 (HSP27) and P21 in nasopharyngeal car- cinoma (NPC) tissue, and to evaluate the significance of both HSP27 and P21 in the pathogenesis, development and prognosis of N... To study the expression of heat stress protein 27 (HSP27) and P21 in nasopharyngeal car- cinoma (NPC) tissue, and to evaluate the significance of both HSP27 and P21 in the pathogenesis, development and prognosis of NPC. Indirect immunofluorescence method combined with SABC was applied. Our results showed that (1) the positive rates of HSP27 and P21 expressed in NPC tissue in 36 cases were 88. 9 % and 94. 4%; (2) while in 10 hyperplastic nasopharyngitis tissues, the positive rate of HSP27 and P21 were both 5; (3) all the 5 normal tissues were negatively stained. It is obvi- ous that a co-expressing tendency of HSP27 and P21 could be identified, and it was associated with the degree of malignancy and the clinical stage of NPC. It is concluded that the positive expression of HSP27 and P21 may have clinical significance in the evaluation of the occurring, development and prognosis of NPC, and in NPC treatment. 展开更多
关键词 nasopharyngeal carcinoma heat stress protein 27 P21
下载PDF
Polymorphism of heat shock protein 70-2 and enterocutaneous fistula in Chinese population 被引量:1
15
作者 Jun Chen Jian-An Ren +8 位作者 Gang Han Guo-Sheng Gu Ge-Fei Wang Xiu-Wen Wu Bo Zhou Dong Hu Yin Wu Yun-Zhao Zhao Jie-Shou Li 《World Journal of Gastroenterology》 SCIE CAS 2014年第35期12559-12565,共7页
AIM: To investigate whether the heat shock protein 70-2 (HSP70-2) polymorphism is associated with enterocutaneous fistulas in a Chinese population.
关键词 Enterocutaneous fistulas Single nucleotide polymorphisms heat shock protein HSP70-2
下载PDF
Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant 被引量:1
16
作者 WanT ZhouX ChenG AnH ChenT ZhangW LiuS JiangY YangF WuY CaoX 《第二军医大学学报》 CAS CSCD 北大核心 2005年第7期771-771,共1页
Heat shock proteins (HSPs) are reported to act as effective adjuvants to elicit anti-tumor and anti-infection immunity. Here, we report that Hsp70-like protein 1 (Hsp70L1), a novel HSP derived from human dendritic cel... Heat shock proteins (HSPs) are reported to act as effective adjuvants to elicit anti-tumor and anti-infection immunity. Here, we report that Hsp70-like protein 1 (Hsp70L1), a novel HSP derived from human dendritic cells (DCs), has potent adjuvant effects that polarize responses toward Th1. With a calculated molecular weight of 54.8 kDa, Hsp70L1 is smaller in size than Hsp70 but resembles it both structurally and functionally. Hsp70L1 shares common receptors on DCs with Hsp70 and can interact with DCs, promoting DC maturation and stimulating secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1beta, tumor necrosis factor-alpha (TNF-alpha), and the chemokines IP-10, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and normal T cell expressed and secreted (RANTES). The induction of interferon-gamma-inducible protein 10 (IP-10) secretion by Hsp70L1 is not shared by Hsp70, and other functional differences include more potent stimulation of DC IL-12p70, CC-chemokine, and CCR7 and CXCR4 expression by Hsp70L1. Immunization of mice with the hybrid peptide Hsp70L1-ovalbumin(OVA)(257-264) induces an OVA(257-264)-specific Th1 response and cytotoxic T lymphocyte (CTL) that results in significant inhibition of E.G7-OVA tumor growth. The ability of Hsp70L1 to activate DCs indicates its potential as a novel adjuvant for use with peptide immunizations; the Hsp70L1 antigen peptide hybrid may serve as a more effective vaccine for the control of cancer and infectious diseases. 展开更多
关键词 Th heat Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant
下载PDF
Cytotoxic T-lymphocytes response in vitro activated by dendritic cells pulsed with heat shock protein 70 derived from human bladder tumor cell lines of EJ 被引量:1
17
作者 Lingfeng He Jianhua Wang +5 位作者 Xiaofeng Wang Xiangjun He Zheng Yan Kexin Xu Kaopeng Guan Shukun Hou 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第12期713-718,共6页
Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lin... Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lines of E J, were able to induce peptide specific cytotoxic T-lymphocytes (CTL) response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods: The E J-derived HSP70 co-cultured with DC from the healthy volunteers' PBMC, along with the crude lysate (the supematant before HSP70 purification) from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes (SI) and interferon-y (IFN-γ) were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51^Cr releasing test. Results: Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate (the supernatant before HSP70 purification) (P 〈 0.05). DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells (P 〈 0.05). Conclusion: The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor. 展开更多
关键词 heat shock protein 70 (HSP70) dendritic cells (DC) cytotoxic T-lymphocytes (CTL) bladder tumor
下载PDF
The Heat Shock Protein Story—From Taking mTORC1,2 and Heat Shock Protein Inhibitors as Therapeutic Measures for Treating Cancers to Development of Cancer Vaccines 被引量:3
18
作者 Peter Chin Wan Fung Regina Kit Chee Kong 《Journal of Cancer Therapy》 2017年第11期962-1029,共68页
Heat shock proteins (HSPs) serve to correct proteins’ conformation, send the damaged proteins for degradation (quality control function). Heat shock factors (HSFs) are their transcription factors. The protein complex... Heat shock proteins (HSPs) serve to correct proteins’ conformation, send the damaged proteins for degradation (quality control function). Heat shock factors (HSFs) are their transcription factors. The protein complexes mTOR1 and 2 (with the same core mTOR), the phosphoinositide-dependent protein kinase-1 (PDK1), the seine/threonine-specific protein kinase (Akt), HSF1, plus their associated proteins form a network participating in protein synthesis, bio-energy generation, signaling for apoptosis with the help of HSPs. A cancer cell synthesizes proteins at fast rate and needs more HSPs to work on quality control. Shutting down this network would lead to cell death. Thus inhibitors of mTOR (mTORI) and inhibitors of HSPs (HSPI) could drive cancer cell to apoptosis—a “passive approach”. On the other hand, HSPs form complexes with polypeptides characteristic of the cancer cells;on excretion from the cell, they becomes antigens for the immunity cells, eventually leading to maturation of the cytotoxic T cells, forming the basic principle of preparing cancer-specific, person-specific vaccine. Recent finding shows that HSP70 can penetrate cancer cell and expel its analog to extracellular region, giving the hope to prepare a non-person-specific vaccine covering a variety of cancers. Activation of anti-cancer immunity is the “active approach”. On the other hand, mild hyperthermia, with increase of intracellular HSPs, has been found to activate the immunity response, and demonstrate anti-cancer effects. There are certain “mysteries” behind the mechanisms of the active and passive approaches. We analyze the mechanisms involved and provide explanations to some mysteries. We also suggest future research to improve our understanding of these two approaches, in which HSPs play many roles. 展开更多
关键词 heat shock proteins and heat shock Factors mTORC1 2 Complexes Mild Hyperthermia ANTI-CANCER Drugs and HSP-Based ANTI-CANCER Vaccine Immunity Cells Trafficking through High Endothelial VENULES of Cancer Site Intrinsic Extrinsic FOXO Translocation and the PERK-CHOP Apoptotic Pathways TYROSINE Kinase Receptors
下载PDF
ACUPUNCTURE EFFECTS ON INDUCIBLE NITRIC OXIDE SYNTHASE mRNA, iNOS PRODUCT AND HEAT SHOCK PROTEIN IN PERITONEAL MACROPHAGES OF THE MOUSE
19
作者 郑乃刚 王一菱 +2 位作者 吴景兰 王红梅 丁一 《World Journal of Acupuncture-Moxibustion》 1998年第4期35-38,共4页
To study the acupuncture effects on the inducible nitric oxide synthase (iNOS) mRNA,iNOS product and heat shock protein(hsp) 70 in the mouse macrophages, after peritoneal stimulation with steriled paraffin oil for 48 ... To study the acupuncture effects on the inducible nitric oxide synthase (iNOS) mRNA,iNOS product and heat shock protein(hsp) 70 in the mouse macrophages, after peritoneal stimulation with steriled paraffin oil for 48 h, 24 Kunming mice were randomly divided into 3 groups: a, electroacupuncture (EA) groupl treated with EA; b, control 1 (C 1) group, peritoneal macrophages treated with culture; and c, control 2 (C 2) group, treated with neither EA nor culture. The macrophages of mice in 3 groups collected from respective peritoneal cavities were prepared into two kinds of specimens, including slide and nitrocellulose membrane (NCM). The iNOS mRNA, iNOS and hsp 70 were detected respectively with in situ hybridization, cytochemistry, immunohistochemistry and RNA and protein dot blots. The results showed that the signals of iNOS mRNA and iNOS product were localized in the macrophage cytoplasm; the immunoreactivity(IR) of hsp 70 localized in both cytoplasm and nucleus. The dot blot signal scanning value of those in 3 groups in comparison with each other showed as follows: EA group>C 2 group>C 1 group, (P<0.01). 展开更多
关键词 MACROPHAGES INOS expression heat shock proteins(hsps) Acupuncture
下载PDF
Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder (Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection
20
作者 YAN Weijie QIAO Yingjie +3 位作者 LIU Yuxiang LIU Xiumei ZHANG Quanqi WANG Xubo 《Journal of Ocean University of China》 SCIE CAS CSCD 2021年第4期886-896,共11页
Heat shock proteins 10/60(hsp10/60)are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions.Besides the chaperone and housekeepin... Heat shock proteins 10/60(hsp10/60)are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions.Besides the chaperone and housekeeping functions,they are also known to be involved in immune response during bacterial infection.In this study,we identified and annotated 10 hsp10/60 genes through bioinformatic analysis in Japanese flounder(Paralichthys olivaceus).Among them one member of hsp10(hspe)family and nine members of hsp60(hspd)family were identified.Phylogenetic and selection pressure analysis showed that the hsp10/60 genes were evolutionarily constrained and their function was conserved.Besides,hsp10/60 genes were involved in different embryonic and larval stages and acted as the sentinel role in an unchallenged organism.In addition,we also observed the expression patterns of hsp10/60 genes after Edwardsiella tarda infection,for the first time in Japanese flounder.Eight out of 10 genes were differentially expressed after bacterial challenges,the significantly regulated expressions of flounder hsp10/60 genes after bacterial infections suggested their involvement in immune response in flounder.Our results provide valuable information for clarifying the evolutionary relationship,and early insights of the immune functions of hsp10/60 genes in Japanese flounder. 展开更多
关键词 heat shock protein Hsp10/60 Japanese flounder Edwardsiella tarda immune response
下载PDF
上一页 1 2 20 下一页 到第
使用帮助 返回顶部