为了分离一株裂解性鸡白痢沙门氏菌噬菌体,对抗多重耐药细菌,试验从健康鸡粪便中分离富集噬菌体,采用双层琼脂平板法纯化、增殖,超速离心法浓缩,利用透射电镜观察噬菌体形态,通过提取噬菌体基因组、酶切、电泳确定核酸类型,同时测定噬...为了分离一株裂解性鸡白痢沙门氏菌噬菌体,对抗多重耐药细菌,试验从健康鸡粪便中分离富集噬菌体,采用双层琼脂平板法纯化、增殖,超速离心法浓缩,利用透射电镜观察噬菌体形态,通过提取噬菌体基因组、酶切、电泳确定核酸类型,同时测定噬菌体的宿主谱、最佳感染复数、一步生长曲线、酸碱及热稳定性等生物学特性。结果表明:从鸡粪中分离得到1株裂解性噬菌体,命名为PSP2-22。该噬菌体头部直径为(60±5)nm,尾部长为(130±5)nm,为长尾噬菌体科;核酸类型为双链DNA。该噬菌体不仅能裂解鸡白痢沙门氏菌,还可裂解鼠伤寒沙门氏菌和奇异变形杆菌;最佳感染复数为0.1;平均裂解量约为136 pfu/cell,效价最高可达9.83 lg pfu/mL;在pH值6~11时相对稳定,70℃作用60 min之后仍具有一定活性。说明噬菌体PSP2-22是一株对不同温度和pH值有较强适应能力的双链DNA噬菌体,宿主谱相对较宽,具有潜在的应用价值。展开更多
The persistent epidemic of human mpox, caused by mpox virus (MPXV), raises concerns about the future spread ofMPXV and other poxviruses. MPXV is a typical zoonotic virus which can infect human and cause smallpox-likes...The persistent epidemic of human mpox, caused by mpox virus (MPXV), raises concerns about the future spread ofMPXV and other poxviruses. MPXV is a typical zoonotic virus which can infect human and cause smallpox-likesymptoms. MPXV belongs to the Poxviridae family, which has a relatively broad host range from arthropods tovertebrates. Cross-species transmission of poxviruses among different hosts has been frequently reported andresulted in numerous epidemics. Poxviruses have a complex linear double-strand DNA genome that encodeshundreds of proteins. Genes related to the host range of poxvirus are called host range genes (HRGs). This reviewbriefly introduces the taxonomy, phylogeny and hosts of poxviruses, and then comprehensively summarizes thecurrent knowledge about the cross-species transmission of poxviruses. In particular, the HRGs of poxvirus aredescribed and their impacts on viral host range are discussed in depth. We hope that this review will provide acomprehensive perspective about the current progress of researches on cross-species transmission and HRGvariation of poxviruses, serving as a valuable reference for academic studies and disease control in the future.展开更多
The (Y,Gd)BO 3∶Eu phosphor was synthesized by solid state reaction. The UV spectra showed that in a certain range of Gd 3+ concentration, more Gd 3+ absorbed energy and transferred it to Eu 3+ with its increasing con...The (Y,Gd)BO 3∶Eu phosphor was synthesized by solid state reaction. The UV spectra showed that in a certain range of Gd 3+ concentration, more Gd 3+ absorbed energy and transferred it to Eu 3+ with its increasing concentration. From the spectra in VUV region, it was observed that both the doping and the concentrations of Gd 3+ , Eu 3+ greatly affected the absorption of the host lattice. The absorbances at 147 nm and 170 nm increased when the Gd 3+ was doped which can be explained as that Gd 3+ transferred energy to BO 4. The optical properties of (Y,Gd)BO 3∶Eu were the best when the concentration of Eu 3+ was about 0.04.展开更多
The molecular recotwtion abilities of β-naphthoated β-CD derivatives as the host compounds were studied: 3-o-β-Naphthoyl-β-CD has a largely improved association constant with cyclohexanol than the corresponding 6-...The molecular recotwtion abilities of β-naphthoated β-CD derivatives as the host compounds were studied: 3-o-β-Naphthoyl-β-CD has a largely improved association constant with cyclohexanol than the corresponding 6-position substituted β-CD derivative has. On the other hand, the polarisablity and the sturcture of the gueset molecule are also important factors which affect the association constant. Cyclohexane may form the 1:2 type inclusion compound with the host compound in this report.展开更多
文摘为了分离一株裂解性鸡白痢沙门氏菌噬菌体,对抗多重耐药细菌,试验从健康鸡粪便中分离富集噬菌体,采用双层琼脂平板法纯化、增殖,超速离心法浓缩,利用透射电镜观察噬菌体形态,通过提取噬菌体基因组、酶切、电泳确定核酸类型,同时测定噬菌体的宿主谱、最佳感染复数、一步生长曲线、酸碱及热稳定性等生物学特性。结果表明:从鸡粪中分离得到1株裂解性噬菌体,命名为PSP2-22。该噬菌体头部直径为(60±5)nm,尾部长为(130±5)nm,为长尾噬菌体科;核酸类型为双链DNA。该噬菌体不仅能裂解鸡白痢沙门氏菌,还可裂解鼠伤寒沙门氏菌和奇异变形杆菌;最佳感染复数为0.1;平均裂解量约为136 pfu/cell,效价最高可达9.83 lg pfu/mL;在pH值6~11时相对稳定,70℃作用60 min之后仍具有一定活性。说明噬菌体PSP2-22是一株对不同温度和pH值有较强适应能力的双链DNA噬菌体,宿主谱相对较宽,具有潜在的应用价值。
基金funded by the National Natural Science Foundation of China(U2002218)the Hunan University Fund(521119400156).
文摘The persistent epidemic of human mpox, caused by mpox virus (MPXV), raises concerns about the future spread ofMPXV and other poxviruses. MPXV is a typical zoonotic virus which can infect human and cause smallpox-likesymptoms. MPXV belongs to the Poxviridae family, which has a relatively broad host range from arthropods tovertebrates. Cross-species transmission of poxviruses among different hosts has been frequently reported andresulted in numerous epidemics. Poxviruses have a complex linear double-strand DNA genome that encodeshundreds of proteins. Genes related to the host range of poxvirus are called host range genes (HRGs). This reviewbriefly introduces the taxonomy, phylogeny and hosts of poxviruses, and then comprehensively summarizes thecurrent knowledge about the cross-species transmission of poxviruses. In particular, the HRGs of poxvirus aredescribed and their impacts on viral host range are discussed in depth. We hope that this review will provide acomprehensive perspective about the current progress of researches on cross-species transmission and HRGvariation of poxviruses, serving as a valuable reference for academic studies and disease control in the future.
文摘The (Y,Gd)BO 3∶Eu phosphor was synthesized by solid state reaction. The UV spectra showed that in a certain range of Gd 3+ concentration, more Gd 3+ absorbed energy and transferred it to Eu 3+ with its increasing concentration. From the spectra in VUV region, it was observed that both the doping and the concentrations of Gd 3+ , Eu 3+ greatly affected the absorption of the host lattice. The absorbances at 147 nm and 170 nm increased when the Gd 3+ was doped which can be explained as that Gd 3+ transferred energy to BO 4. The optical properties of (Y,Gd)BO 3∶Eu were the best when the concentration of Eu 3+ was about 0.04.
文摘The molecular recotwtion abilities of β-naphthoated β-CD derivatives as the host compounds were studied: 3-o-β-Naphthoyl-β-CD has a largely improved association constant with cyclohexanol than the corresponding 6-position substituted β-CD derivative has. On the other hand, the polarisablity and the sturcture of the gueset molecule are also important factors which affect the association constant. Cyclohexane may form the 1:2 type inclusion compound with the host compound in this report.
