Three-dimensional S-wave velocity structure of the upper crust in the Guangdong-Hong Kong-Macao Greater Bay Area:insights into the basins structure and genesis of hot springs

As one of the four largest bay areas with strong economic activities in the world,the Guangdong-Hong Kong-Macao Greater Bay Area(GHMGBA)is located in the zone of interaction between the South China Block(SCB)and the South China Sea(SCS).Under the influence of complex geologic evolution,basin-range structures,fault systems and hot springs are well developed here.However,the characteristics of geological structures and the genetic mechanism of these geological phenomena are still unclear.Therefore,we performed ambient noise tomography to obtain 3-D upper crust(0-7.5 km)S-wave velocity structures of the GHMGBA by using 40-day continuous waveform data from 130 seismic stations in the GHMGBA.Our results show that sedimentary basins in the GHMGBA are mainly characterized by low-velocity anomalies.S-wave velocities of sediment formation in basins are about 2.8-3.1 km/s.Rapid changes in velocity appear at the edges of the basins,which correspond to the NE-,NEE-,and NW-trending faults,indicating prominent basin-controlling effects of the faults.The Sanshui Basin(SSB),the largest in the GHMGBA,has a developmental depth of about 4 km,and there is a significant difference in velocity gradient between the east and west sides of the basin,indicating that SSB has experienced east-west asymmetric expansion.Moreover,there are prominent low-velocity anomalies at a depth of about 4.5 km beneath the hot springs at the west of the Zhujiang(Pearl)River estuary(ZRE).We infer that the low-velocity anomalies are fluid reservoirs of the hot springs,which lead to the development of the hot springs on the surface.In addition,the distribution of main cities in the GHMGBA shows a spatial correlation with low-velocity areas at shallow depths(<3 km).The population development trend in the GHMGBA in the past 20 years is also mainly concentrated in the structural province of relatively low-velocity.In combination with the GHMGBA basin structures and drainage distribution characteristics,we suggest that the basic geological environment to some extent affects the habitability of the human settlement and thus determines the distribution and development trend of the main urban context.We believe that the 3-D S-wave velocity structure of the upper crust of the GHMGBA obtained in this study,as well as the deep structural characteristics of the basins and hot springs,will provide support to urban construction planning and geological hazards research of the GHMGBA.

Evaluation of Pencil Lead Based Electrodes for Electrocardiogram Monitoring in Hot Spring

Electrocardiogram(ECG)electrodes are conductive pads applied to the skin to measure cardiac activity.Ag/AgCl electrodes are the commercial product which widely used to obtain ECGs.When monitoring the ECG in a hot spring,Ag/AgCl electrodes must be waterproofed;however,this is time-consuming,and the adhesive may tear the skin on removal.For solving the problem,we developed the carbon pencil lead(CPL)electrodes for use in hot springs.Both CPL and Ag/AgCl electrodes were connected to ECG100C’s cables.The Performance was evaluated in three conditions as following:hot spring water with and without bubble,and in cold water.In each environment,the procedure was followed by three different protocols that are recording from the dry condition,hot spring water immersion with and without movement,post hot spring water condition.Under dry and wet conditions,both electrodes can obtain the waveform of the ECG signal in which all PQRST waves were identifiable.Nevertheless,the signal quality of both types of electrodes was different in water immersion with and without movement.The overall morphology obtained by Ag/AgCl electrodes was unstable higher than that of CPL electrodes in immersion without movement condition.The CPL electrodes provided better ECG waveform quality compared to Ag/AgCl electrodes in which the ECG signal had high waveforms distortion in water immersion with movement condition.

Influence of the Chuxiong Yao'an Earthquake on the Mineralization of Hot Springs in the Tengchong Geothermal Area,Southwestern China

The Tengchong geothermal area,an active tectonic region with frequent earthquakes,is located in Yunnan Province of southwestern China.This area contains abundant active hot springs, which often display high metal concentrations and obvious mineralization phenomena.At 19：19 on 9 July 2009,an earthquake occurred in Yao＇an,Chuxiong,Yunnan Province,which is 300 km to the northeast of the Tengchong geothermal area.We sampled water in the hot springs in the Tengchong area from 4 July to 9 July 2009 and from 10 July to 15 July 2009 to study the changes of elemental concentrations before and after the earthquake and discuss the influence of the earthquake on the mineralization of the hot springs.The concentrations of most trace elements increased slightly,but the concentration of REE（rare earth elements） decreased by 50%after the earthquake in the hot springs around a NS-trending fault.The elemental concentrations remained unchanged in Longtan and Suanshuigou,which are related to an inactive crater.The elemental concentrations in other springs controlled by superficial and small-scale faults decreased after the earthquake.The earthquake can stimulate the activity of deep faults and magma chambers,as is responsible for the increase of metal concentrations in the hot springs along the NS-trending deep fault;whereas it can decrease the porosity of permeable rocks,resulting in the decline of the flux of ore-bearing fluids and the corresponding mineralization in the hot springs related to superficial faults.

Screening and Characterization of the Alkaline Protease Isolated from PLI-1, a Strain of Brevibacillus sp. Collected from Indonesia's Hot Springs

A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia's hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability.

Biomediated Precipitation of Calcium Carbonate in a Slightly Acidic Hot Spring,Yunnan Province

A slightly acidic hot spring named ＂Female Tower＂（t=73.5°C, pH=6.64） is located in the Jifei Geothermal Field, Yunnan Province, southwestern China. The precipitates in the hot spring are composed of large amounts of calcite, aragonite and sulfur. Scanning electron microscopy（SEM） analyses reveal that the microbial mats were formed from various coccoid or rod-shaped filamentous microbes. Transmission electron microscopy（TEM） shows that the intracellular sulfur granules are commonly associated with these microbes. A culture-independent molecular phylogenetic analysis demonstrates that the majority of the bacteria in the spring are sulfur-oxidizing bacteria. In the spring water, H2S concentration is up to 60 ppm, while SO4-（2-） concentration is only about 10 ppm. We speculate that H2S might derive from sulfur-oxidizing bacteria in this hot spring water, leading to the intracellular formation of sulfur granules. Meanwhile, this reaction increased the p H in the micronscale microdomains, which fosters the precipitation of calcium carbonate in the microbial mats. The results of this study indicate that the sulfur-oxidizing bacteria might play an important role in calcium carbonate precipitation in slightly acidic hot spring environments.

Distribution of glycerol dialkyl glycerol tetraethers in Tibetan hot springs

Isoprenoidal glycerol dialkyl glycerol tetraethers （iGDGTs） from the Gulu hot springs （23--83.6 ℃, pH 〉 7） and Yangbajing hot springs （80-128 ℃, pH 〉 7） were analyzed in order to investigate the distribution of archaeal lipids among different hot springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in hot springs. The sources of bacterial GDGTs （bGDGTs） in these hot springs were also investigated. The results revealed different profiles ofiGDGTs between Gulu and Yangbajing hot springs. Core iGDGTs and polar iGDGTs also presented different patterns in each hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the hot springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan hot springs.

Microbial Mats in Tibetan Hot Springs and their Contributions to the Cesium-bearing Geyserite Ore Formation

Microbial mats, mainly dominated by filamentous algae Calothrix and Oscillatoria, are well developed in Tibetan hot springs. A great number of fossil microorganisms, which existed as algae lamination in thermal depositional cesium-bearing geyserite in this area, are identified as Calothrix and Oscillatoria through microexamination and culture experiments. These microbial mats show the ability to accumulate cesium from spring water to the extent of cesium concentration of 0.46-1.03% cell dry weight, 900 times higher than that in water, and capture large numbers of cesium-bearing opal grain. Silicon dioxide colloid in spring water replaces and fills with the organism and deposits on it to form algae laminated geyserite after dehydration and congelation. Cesium in the microbial mats and opal grain is then reserved in the geyserite. Eventually, cesium-bearing algae laminated geyserite is formed. Study on cesium distribution in geyserite also shows that cesium content in algae lamination, especially in heavily compacted algae lamination, is higher than in the opal layer. For geyserite with no algae lamination or other organism structure, which is generally formed in spring water with low silicon content, cesium accumulation and cesium-bearing opal grain assembled by the microbial mats are also indispensable. After the microbial mats accumulating cesium from spring water, silicon dioxide colloid poorly replaces and fills with the organism to form opal grain-bearing tremellose microbial mats. The shape and structure of the organisms are then destroyed, resulting in cesium-bearing geyserite with no algae lamination structure after dehydration and congelation. It is then concluded that microbial mats in the spring area contribute to the enrichment of cesium in the formation of cesium-bearing geyserite, and a biological genesis of the geyserite, besides of the physical and chemical genesis, is likely.

Research on Tourism Master Planning of Huangshan Hot Spring Scenic Area in China

Based on tourist resources' evaluation and tourism market development of Huangshan Hot Spring Scenic Area,by combining with relevant theories of "SWOT",the paper has identif ied the leading functional orientation and tourism development idea of the hot spring scenic area.Meanwhile,by following the principle of "sustainable use" of Huangshan natural resources,planning strategies and implementing suggestions have been put forward based on "addition,subtraction,multiplication and division".The ultimate purpose is to create diversified tourism experiences for tourists and improve tour quality of hot spring scenic area,and f inally build Huangshan tourism brand of "Snowy Hot Spring".

Study on Utilization and Development of Hot Spring in Liaocheng City

The basic situation and formation mechanism of hot spring in Liaocheng were introduced,and its utilization value as well as exploitation and utilization status were also analyzed in this study,which provided strategies for exploitation and utilization of hot spring in Liaocheng.

Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis

Background: Use of novel microorganisms for beneficial purposes is still remaining a challenging job. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot spring of Metaldanga, Birbhum, West Bengal, India. Methods: A pure bacterial strain (MDH1) was identified by growing the enrichment culture isolated from Metaldanga hot spring through serial dilution process in a semi-synthetic medium at pH 8.0 and 42&deg;C temperature. The novelty of the strain was characterized by 16S-rRNA gene sequence analysis. The bacterium acted as template to synthesize spherical gold nanoparticles (GNPs). GNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infra-red spectroscopy (FTIR). Results: The phylogenetic analysis suggested that MDH1 strain (GenBank accession number: KT600031) was affiliated to the family “Pseudomonadaceae” with 99% homologous to Pseudomonas putida H8234. The coccoid shaped bacterium was gram-negative and facultative-anaerobic which acted as a template to synthesize spherical GNPs with an average size of 12 ± 3 nm when examined under transmission electron microscopy (TEM). FT-IR studies revealed the presence of bioactive functional groups which acted as capping and stabilizing agents of the GNPs. XRD pattern confirmed the amorphous nature of GNPs. The Zeta potential (ζ) concluded the adequate stability of GNPs in an aqueous environment. Conclusions: The present investigation explores the microbial diversity of a virgin hot spring of Metaldanga for its beneficial applications in industry, particularly in the synthesis of the gold nanoparticles.

Evaluation of Tourist's Satisfaction Degree of Dongda Hot Spring Resort Area in Xi'an City

Tourist's satisfaction degree is one of the important indexes to evaluate the service quality of scenic spot.Taking Xi'an Dongda Hot Spring Resort Area for an example,with the expectation difference theory and customers' satisfaction evaluation model as the theoretical basis of the research,the overall satisfaction degree of tourists to hot spring resort area,and the actual sensation accomplishments and the expectation difference of each service index were analyzed by using regression analysis and paired sample t test method.The results showed:① The overall satisfaction degree of tourists to the resort area was higher.There were remarkable correlation among three variables:the sensation value,the overall satisfaction degree,and the tourist's loyalty degree;② Generally,the sensation actual accomplishments of 15 service appraisal factors were higher than the expectation value.According to the characteristics,these 15 service appraisal factors were divided into 5 groups.Some unsatisfactory aspects were found out,and the corrective measures were put forward.

Ultrastructural Evidence for a Novel Accumulation of Ca in a Microbial Mat from a Slight Acidic Hot Spring

Microbial mats are ecosystems that can control or induce the precipitation of calcium（Ca） carbonate on Earth through geological time.In the present study,we report on a novel accumulation of Ca,together with iron（Fe）,in a microbial mat collected from a slight acidic hot spring（pH=5.9） in south China.Combining an array of approaches,including environmental scanning electron microscopy,X-ray microanalysis,transmission electron microscopy,and selected area electron diffraction,we provide ultrastructral evidence for amorphous acicular aggregates containing Ca and Fe associated with cyanobacteria precipitating in the microbial mats.Cyanobacterial photosynthesis and exopolymeric organic matrixes are considered to be responsible for the precipitation of Ca.These amorphous acicular aggregates might imply the early stage of calcification occurring in microbial mats.Ca and Fe coprecipitation indicates another potential important way of inorganic element precipitation in hot spring microbial mats.Our results provide insight into the possible mechanism of cyanobacterial calcification and microfossil preservation in slight acidic hot spring environments.

A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments.The hot springs of Xiamen Island are close to the Xiamen Sea,and may have some characteristics different from those of inland hot springs.Microbes living in the hot springs of Xiamen may have new characteristics.However,little is known about microbial communities of hot springs close to the Xiamen Sea.A culture-dependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing.A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs,including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles.Neutrophilic thermophilic bacteria,which grow at a temperature range of 55-90℃ including Rhodothermus marinus（Strain 1）,Thermus thermophilus（Strain 2）,Thermus thiopara（Strain 3）,Geobacillus stearothermophilus（Strain 4）,Geobacillus thermoleovorans（Strain 5）,and Pseudomonas pseudoalcaligenes（Strain 6）,were recovered by 2216E plates.Moderately thermophilic acidophiles,which can grow at temperatures above 50℃ and a pH range of 1.8-3.5 such as Alicyclobacillus acidoterrestris（Strain 8）,Sulfobacillus acidophilus（Strain 9）,and Sulfobacillus thermosulfidooxidans（Strain 10）,were isolated on selective solid medium containing sulfur and Fe2＋.Among these strains,Rhodothermus marinus,Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles,but also halophiles.One bacterium strain（Strain 6） shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence,but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics,suggesting that it may represent a novel thermophilic species.Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.

Mathematical Model of the Geothermal Water Resources in the South Hot Spring System in Chongqing

The geothermal waters of south hot spring, small hot spring and Qiaokouba in Chongqing, are all part of the south hot spring geothermal water system. Exploitation has caused a decline in the water levels of the south and small hot springs, which have not flowed naturally for 15 years. Now, bores pump geothermal water to the springs. If the water level drops below the elevation of the rivers, river-water will replenish the geothermal water, destroying this resource. It is therefore an urgent task to model the geothermal water system, to enable sustainable development and continued use of the geothermal water in Qiaokouba. A numerical simulation of the geothermal water system was adopted and a quantitative study on the planning scheme was carried out. A mathematical model was set up to simulate the whole geothermal water system, based on data from the research sites. The model determined the maximum sustainable water yield in Qiaokouba and the two hot springs, and the south hot spring and small hot spring sustainable yields are 1 100 m^3/d and 700 m^3/d from 2006 to 2010, 1 300 m^3/d and 1 000 m^3/d from 2011 to 2015, and 1 500 m^3/d and 1 200 m^3/d from 2016 to 2036. The maximum exploitable yield is 3 300 m^3/d from 2006 to 2036 in Qiaokouba. The model supplies a basis to adequately exploit and effectively protect the geothermal water resources, and to continue to develop the geothermal water as a tourist attraction in Chongqing.

Subaerial hot springs and near-surface hydrothermal mineral systems past and present,and possible extraterrestrial analogues

The principal features,geotectonic settings and association with near-surface hydrothermal mineral systems of ancient and present subaerial hot springs,fumaroles and geysers are herein reviewed.Fumaroles and geysers usually occur in volcanic craters and are in most cases,part and parcel of hot spring environments.Subaerial hot springs are characterised by siliceous-and carbonate-rich chemical sediments,such as sinters and travertines,respectively.Sinters are commonly enriched in various metalliferous elements.Hot springs surface discharges are also characterised by pools,which exhibit bright colours due to the presence of microorganisms.Present-day examples discussed in this paper,include the fumaroles and hot springs of the White Island volcano(New Zealand),the world-renowned Yellowstone caldera(USA)and the Afar region of the East African Rift System.The Afar triangle,in the northern part of East African Rift System,provides a good example of hot springs associated with evaporative deposits.The Tuli-Sabi-Lebombo triple junction rifts were formed during the^180 Ma Karoo igneous event in southern Africa,of which the Tuli arm is the failed rift(aulacogen),as is the Afar region.The Tuli rift is effectively an unusual,if not unique,tectono-thermal setting,because it comprises Karoo-age hot springs systems and associated vein stockworks and breccias,which includes the Messina Cu deposit as well as currently active hot springs with sinter deposits probably due to post-Karoo uplift.Fumarolic pipes in ignimbrites of the Erongo Volcano-Plutonic Complex in Namibia are discussed.This is followed by an example of banded chert rocks in the Killara Formation of the Palaeoproterozoic Capricorn Orogen(Western Australia),interpreted as hot spring chemical sediments,which also show evidence of"fossil"microbial filaments.The paper ends with a brief overview of possible analogues of hot springs on planet Mars.

Exploitation and Sustainable Development of Hot Springs in Xinjiang Province of China——A Case Study of Shawan Hot Spring Tourist Area

The paper had introduced geographic causes and types of hot springs, illustrated its disease-treating function and displayed its great vitality and widespread development prospect as tourist resources. By taking Shawan Hot Spring in Xinjiang for example, the paper had analyzed its natural condition, hot springs and tourist economic development. And then, quantitative and qualitative analysis had been conducted on the quality of water environment resources in the research area. It had analyzed reasons for degeneration of water environment and studied exploitation and sustainable development of hot springs. It was considered that environment especially water environment quality had decreased since hot spring tourism development. In order to achieve sustainable development of hot springs and promotion of local tourism, it must be guided by environment treatment, and some strategies for sustainable development of hot springs had been proposed.

Design and Practice of Digital Value-Added in Jiaosi Hot Spring Tourism Industry

With an aim to promote the digital value-added design of local tourism, the study adopted Skilbeck＇s situational model to examine the promotion of local tourism through a digital value-added approach and integrated the design of APP： Tour Guide for Jiaoxi Hot Spring into the Study of Technology and Applications of Mobile Services and Augmented Reality for Cultural and Creative Industry and Tourism in Yilan. Through the development and demonstration of design, students are allowed to build and enhance their capacity for integration in addition to fulfilling university social responsibility （USR）. The study found that students used different methods in each phase to continuously absorb knowledge and discover problems and then propose creative solutions based on facts. By creating a mental map, students pondered promoting local tourism via digital value-added design and came up with a strategy of hot spring ＋ primary industry （agriculture ＋ aquaculture） tours, which integrated local gourmet, hot springs, religion, and the hot spring museum. Three routes are designed, namely Route 1： Hot Spring Park ＋ Aquaculture, Route 2： Tangweigou Hot Spring Park, and Route 3： Jiaoxi Old Street to make up the gap in the unilateral marketing of Jiaoxi Hot Spring. The study will further promote location-based services of the APP to fulfill the technology and applications of mobile services and augmented reality for local tourism.

The Potential of Hot Springs in the Westem Thailand for Health Tourism and Sustainability

This research aims to examine the potential of hot spring sites in the Western Thailand for the purpose of f sustainable tourism. The research applied qualitative methods and the participation of communities was included in the study＇s approach. The fieldwork consisted of the development of in-depth interviews, focus groups, field survey, and participation observation. Participants were stakeholders in tourism development. The data collected from interviews were analysed, interpreted, and reported with content and descriptive analysis. It was found that the hot spring destinations had their outstanding resources, based on their natural surrounded. The resources were used for tourism purpose in terms of health tourism. The potential of the various hot spring areas was divided into four groups. The first group included hot spring location where tourism data had not been gathered. The second group encompassed hot spring where tourism data had been gathered. The third group was hot springs where some tourism details had been gathered, but the hot spring sites were faced with some problems. The last group included well-developed hot spring locations where tourism data had been gathered before. All locations could be promoted for health tourism, and educational tourism could be added for tourist and the local community. Such development would need to be controlled by the community committees formed by representatives of all stakeholders directly and indirectly related to tourism development. Tourism development generated by community committees can result in sustainable development of hot spring destinations.

Microbial Mediated Mineralizationin Kamchatka Hot Springs

Materials deposited in low-temperature environments are characterized by their small particle size.The activity of microorganisms in aqueous sedimentary environments may have effective impacts on the geochemical

Removal of Lead Contamination through the Formation of Lead-Nanoplates by a Hot Spring Microbial Protein

Lead contamination still remains as serious threat to public health and environment because of its non-biodegradability and toxicity. A clean technique has been developed for removal of lead contamination through the formation of lead-oxide nanoplates using a bacterial protein (Molecular weight ~30 kDa) as biological template. The isolated hot-spring bacterial (the bacterium was named as MDH1) protein when adding to the solution of lead compound (e.g., lead nitrate), nanoplates of lead-oxide are formed as viewed by electron microscope. The as prepared lead-oxide-nanoplates are characterized by Inductively Coupled Plasma analysis, Energy Dispersive X-ray Spectroscopy and X-ray diffraction analyses. The lead-oxide-nanoplates and the filtered supernatant of the reactive solution both were separately used to observe the inhibition of growth of E. coli bacteria on culture plate. Lead-oxide-nanoplates produced clear zone of inhibition on the bacterial growth plate, whereas the filtered supernatant exhibited no such zone on the growth of E. coli bacteria revealing the fact that lead contamination was removed from the filtered supernatant. The prepared lead oxide nanoplates also possess dye degradation activity which is the added advantage of the process. The MDH1 bacterial protein acts as biological template which successfully removes lead contamination from lead-solution. The process is a clean and cost-effective one which can be used not only for removal of lead contamination but also for removal of different dyes from environment due to having dye-degradation attribute of the lead-oxide nanoplates.