One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both prote...One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory network.To detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were developed.Screening the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this system.One TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis regulation.Electrophoretic mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional regulation.The CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2homeostasis.The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.In addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.展开更多
采用溶胶-凝胶法制备Y0.06Sr0.94Ti1-xFexO3-δ（x为原子分数。x=0.2，0.3，0.4，0.5）混合导体材料，用X射线衍射（XRD）分析该材料的物相，采用交流阻抗法和电子阻塞电极法分别测定其总电导率与离子电导率，研究铁含量对Y0.06Sr0.94T...采用溶胶-凝胶法制备Y0.06Sr0.94Ti1-xFexO3-δ（x为原子分数。x=0.2，0.3，0.4，0.5）混合导体材料，用X射线衍射（XRD）分析该材料的物相，采用交流阻抗法和电子阻塞电极法分别测定其总电导率与离子电导率，研究铁含量对Y0.06Sr0.94TiO3混合导体材料的结构及电性能的影响。结果表明该材料属于单一立方相钙钛矿结构；在测试温度范围内，Y0.06Sr0.94Ti1-xFexO3-δ的总电导率和离子电导率都随温度升高而增大；随 Fe 掺杂量增加，总电导率和离子电导率都增大。在800℃下Y0.06Sr0.94Ti1-xFexO3-δ（x=0.2，0.3，0.4，0.5）的总电导率为0.019～0.12 S/cm，离子电导率为0.0106～0.0153 S/cm。根据传导活化能可以看出，x从0.3增加到0.4时材料的传导机制发生改变。展开更多
红金麻 新杂种 Hibiscus×hongjinma Y. T. Chang, hybr. nov. H. cannabinus L. ♀× H. radiatus Cav.♂ Hybrida hortensis ( 2n=30+6sat) θ Hibisco cannabino L. ( 2n=32+4sat) et H. ranndiato Cav. (2n=70+2sat) exorta...红金麻 新杂种 Hibiscus×hongjinma Y. T. Chang, hybr. nov. H. cannabinus L. ♀× H. radiatus Cav.♂ Hybrida hortensis ( 2n=30+6sat) θ Hibisco cannabino L. ( 2n=32+4sat) et H. ranndiato Cav. (2n=70+2sat) exorta, Habitu illo simil, characteribus ad hoc magis accedit; ab illo differt floribus purpureis, folio prope basin展开更多
为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同...为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同等施氮量(180 kg N.hm-2)的SCU和普通尿素相比,增产幅度为13.2%;施用135 kg N.hm-2的SCU与180 kg N.hm-2普通尿素相比(氮素用量减少25%)仍可增产12.9%。稻田施氮量可显著影响土壤中铵态氮的累积和分布,但对硝态氮的影响较小。折合等量纯氮的SCU与普通尿素处理相比,可以显著减少肥料施用初期土壤水中氮素含量,施肥后10 d,SCU处理农田面水、0～20 cm层和20～40 cm层土壤水全氮含量分别为施用普通尿素处理的26.3%、64.4%和64.0%。展开更多
Three polysaccharides （EW, EH and EA） were prepared from a red alga Eucheuma denticulatum by sequential extraction with cold water, hot water and sodium hydroxide water solution. Their monosaccharide compositions, r...Three polysaccharides （EW, EH and EA） were prepared from a red alga Eucheuma denticulatum by sequential extraction with cold water, hot water and sodium hydroxide water solution. Their monosaccharide compositions, relative molecular mass and structural characterization were determined by gas chromatography, high performance liquid chromatography, fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy methods. EW was hybrid l/k/v-carrageenan （701/17k/13v-car- rabiose）, EH was mainly t-carrageenan, and EA was mainly α-1,4-Glucan （88%） but mixed with small amount of t-carrageenan （12%）. The relative molecular mass ofEW, EH and EA was 480, 580 and 510kDa, respectively. The anti-influenza A （H1N1） virus activity of these three polysaccharides was evaluated using the Madin-Darby canine kidney cells model. EW showed good anti-H1N1 virus activity, its ICso was 276.5 μg mL-1, and the inhibition rate to H1N1 virus was 52% when its concentration was 250 μgmL-1. The ICs0 of t-carrageenan EH was 366.4 μgmL1, whereas EA showed lower anti-H1N1 virus activity （IC50〉430μgmL-1）. Available data obtained give positive evidence that the hybrid carrageenan EW from Eueheuma denticulatum can be used as potential anti-H1N1 virus inhibitor in future.展开更多
基金funded by the National Key Research and Development Program of China(2022YFD1201600)the earmarked fund for the China Agriculture Research System(CARS-26)+1 种基金the Fundamental Research Funds for the Central Universities,China(SWU-XDJH202308)the Science and Technology Research Program of Chongqing Municipal Education Commission,China(KJQN202001418)。
文摘One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory network.To detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were developed.Screening the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this system.One TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis regulation.Electrophoretic mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional regulation.The CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2homeostasis.The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.In addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.
文摘采用溶胶-凝胶法制备Y0.06Sr0.94Ti1-xFexO3-δ（x为原子分数。x=0.2，0.3，0.4，0.5）混合导体材料，用X射线衍射（XRD）分析该材料的物相，采用交流阻抗法和电子阻塞电极法分别测定其总电导率与离子电导率，研究铁含量对Y0.06Sr0.94TiO3混合导体材料的结构及电性能的影响。结果表明该材料属于单一立方相钙钛矿结构；在测试温度范围内，Y0.06Sr0.94Ti1-xFexO3-δ的总电导率和离子电导率都随温度升高而增大；随 Fe 掺杂量增加，总电导率和离子电导率都增大。在800℃下Y0.06Sr0.94Ti1-xFexO3-δ（x=0.2，0.3，0.4，0.5）的总电导率为0.019～0.12 S/cm，离子电导率为0.0106～0.0153 S/cm。根据传导活化能可以看出，x从0.3增加到0.4时材料的传导机制发生改变。
文摘红金麻 新杂种 Hibiscus×hongjinma Y. T. Chang, hybr. nov. H. cannabinus L. ♀× H. radiatus Cav.♂ Hybrida hortensis ( 2n=30+6sat) θ Hibisco cannabino L. ( 2n=32+4sat) et H. ranndiato Cav. (2n=70+2sat) exorta, Habitu illo simil, characteribus ad hoc magis accedit; ab illo differt floribus purpureis, folio prope basin
文摘为探讨超级杂交中稻超高产栽培的缓释尿素(硫包衣尿素,SCU)最佳施用量及其环境效应,在大田试验条件下,研究了SCU不同用量对超级杂交中稻Y两优1号产量以及对土水中氮素含量的影响。结果表明,与普通尿素相比,施用SCU有显著的增产效果,同等施氮量(180 kg N.hm-2)的SCU和普通尿素相比,增产幅度为13.2%;施用135 kg N.hm-2的SCU与180 kg N.hm-2普通尿素相比(氮素用量减少25%)仍可增产12.9%。稻田施氮量可显著影响土壤中铵态氮的累积和分布,但对硝态氮的影响较小。折合等量纯氮的SCU与普通尿素处理相比,可以显著减少肥料施用初期土壤水中氮素含量,施肥后10 d,SCU处理农田面水、0～20 cm层和20～40 cm层土壤水全氮含量分别为施用普通尿素处理的26.3%、64.4%和64.0%。
基金supported by International Science and Technology Collaboration Program of China (2007DFA-30980)Program for Changjiang Scholars,Innovative Research Team in University (IRT0944)+1 种基金Natural Science Foundation of China (31070724)Special Fund for Marine Scientific Research in the Public Interest (201005024)
文摘Three polysaccharides （EW, EH and EA） were prepared from a red alga Eucheuma denticulatum by sequential extraction with cold water, hot water and sodium hydroxide water solution. Their monosaccharide compositions, relative molecular mass and structural characterization were determined by gas chromatography, high performance liquid chromatography, fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy methods. EW was hybrid l/k/v-carrageenan （701/17k/13v-car- rabiose）, EH was mainly t-carrageenan, and EA was mainly α-1,4-Glucan （88%） but mixed with small amount of t-carrageenan （12%）. The relative molecular mass ofEW, EH and EA was 480, 580 and 510kDa, respectively. The anti-influenza A （H1N1） virus activity of these three polysaccharides was evaluated using the Madin-Darby canine kidney cells model. EW showed good anti-H1N1 virus activity, its ICso was 276.5 μg mL-1, and the inhibition rate to H1N1 virus was 52% when its concentration was 250 μgmL-1. The ICs0 of t-carrageenan EH was 366.4 μgmL1, whereas EA showed lower anti-H1N1 virus activity （IC50〉430μgmL-1）. Available data obtained give positive evidence that the hybrid carrageenan EW from Eueheuma denticulatum can be used as potential anti-H1N1 virus inhibitor in future.
