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Hybridization between salt resistant and salt susceptible genotypes of mungbean(Vigna radiata L.Wilczek)and purity testing of the hybrids using SSRs markers 被引量:3
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作者 Sehrawat Nirmala Yadav Mukesh +2 位作者 Bhat Kangila Venkataraman Sairam Raj Kumar Jaiwal Pawan Kumar 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第3期521-527,共7页
Six cultivated and two wild genotypes of mungbean(Vigna radiata L. Wilczek) possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod ... Six cultivated and two wild genotypes of mungbean(Vigna radiata L. Wilczek) possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12%(average 3.08%). Molecular analysis confirmed the hybrids purity. All F_1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F_2 seeds ranged from 5–8 per cluster. The F_2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening. 展开更多
关键词 mungbean salinity stress breeding hybrid purity improved characteristics
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RAPD Appraisal of Purity of the Hybrid Rice SY63 被引量:1
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作者 陈洪 钱前 +2 位作者 朱立煌 闵绍楷 陈美玲 《Developmental and Reproductive Biology》 1996年第2期60-66,共7页
使用纯度低或真实性差的杂交种子会给农业生产造成极大损失。然而杂交种子的纯度无法单纯从种子形态上鉴别.本文首次采用RAPD特异扩增谱带做为分子标记对水稻杂交种纯度进行了鉴定。汕优63杂交水稻种子由中国水稻研究所从某制种... 使用纯度低或真实性差的杂交种子会给农业生产造成极大损失。然而杂交种子的纯度无法单纯从种子形态上鉴别.本文首次采用RAPD特异扩增谱带做为分子标记对水稻杂交种纯度进行了鉴定。汕优63杂交水稻种子由中国水稻研究所从某制种单位随机取样获得。珍汕97A不育系和明恢63恢复系等品种由中国水稻研究所提供。在做形态观察的同时,分别取上述三种材料叶片制备DNA作为初始模板DNA。对300个RAPD随机引物,经过三次多态性初筛复筛,发现随机引物P18可以稳定地扩增出一条来源于父本明恢63的0.8kb的特异条带。用P18引物对100株汕优63杂交单株进行RAPD扩增,其中83个获得了0.8kb特异条带(Fig.1upper)。分子杂交证明其结果是可靠的(Fig.1Lower)。以RAPD扩增结果为依据,对这100株材料进行植株形态比较,发现那17个不能扩增出特异条带的单株均为假杂种。说明该制种单位汕优63的假种率为17%。大大超过农业部的有关规定。应对其原因和后果进行追究。用P18引物对18种分别为籼粳及其中间型的常用稻种进行RAPD扩增鉴定,除明恢63的亲本──圭630和中间型Pccos,Aus373具有0.8kb的特异条带? 展开更多
关键词 hybrid rice purity of a hybrid RAPD Appraisal.
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A new method to identify and improve the purity of hybrid rice with herbicide resistant gene 被引量:3
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作者 HUANG Danian,ZHANG Shanging,XUE Rui,HUA Zhihua,XIU Xiaobo,and WANG Xiaoling,CNRRI,Hangzhou 310006,China 《Chinese Rice Research Newsletter》 1998年第1期1-1,共1页
There is a close relationship between the hy—brid rice production and seed purity.Two-linehybrid rice with higher heterosis is producedthrough the hybridization between a photo-thermo sensitive genetic male sterile(G... There is a close relationship between the hy—brid rice production and seed purity.Two-linehybrid rice with higher heterosis is producedthrough the hybridization between a photo-thermo sensitive genetic male sterile(GMS) rice line and a paternal variety.But the fertili- 展开更多
关键词 A new method to identify and improve the purity of hybrid rice with herbicide resistant gene
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