AIM: To report the 3mo outcomes of collagen crosslinking(CXL) with a hypo-osmolar riboflavin in thin corneas with the thinnest thickness less than 400 μm without epithelium.METHODS: Eight eyes in 6 patients with age ...AIM: To report the 3mo outcomes of collagen crosslinking(CXL) with a hypo-osmolar riboflavin in thin corneas with the thinnest thickness less than 400 μm without epithelium.METHODS: Eight eyes in 6 patients with age 26.2±4.8y were included in the study. All patients underwent CXL using a hypo-osmolar riboflavin solution after its de-epithelization. Best corrected visual acuity, manifest refraction, the thinnest corneal thickness, and endothelial cell density were evaluated before and 3mo after the procedure.RESULTS: The mean thinnest thickness of the cornea was 408.5 ±29.0 μm before treatment and reduced to369.8 ±24.8 μm after the removal of epithelium. With the application of the hypo-osmolar riboflavin solution, the thickness increased to 445.0 ±26.5 μm before CXL and recover to 412.5 ±22.7 μm at 3mo after treatment, P =0.659). Before surgery, the mean K-value of the apex of the keratoconus corneas was 57.6 ±4.0 diopters, and slightly decreased(54.7±4.9 diopters) after surgery(P =0.085). Mean best-corrected visual acuity was 0.55 ±0.23 logarithm of the minimal angle of resolution, and increased to 0.53±0.26 logarithm after surgery(P =0.879).The endothelial cell density was 2706.4 ±201.6 cells/mm2 before treatment, and slightly decreased( 2641. 2 ±218.2 cells/mm2) at last fellow up(P =0.002).CONCLUSION: Corneal collagen cross-linking with a hypo-osmolar riboflavin in thin corneas seems to be a promising treatment. Further study should be done to evaluate the safety and efficiency of CXL in thin corneas for the long-term.展开更多
Saccharomyces cerevisiae osmotic fragile mutants were obtained from parental strain SH208 15 by N methyl N’ nitro N nitrosoguanidine(NTG) treatments. Three mutants, HF2, HF7 and HF9 showed high degree of release of t...Saccharomyces cerevisiae osmotic fragile mutants were obtained from parental strain SH208 15 by N methyl N’ nitro N nitrosoguanidine(NTG) treatments. Three mutants, HF2, HF7 and HF9 showed high degree of release of the intracellular components into medium under hypo osmotic condition and were studied in details. They showed some similar phenotypes to the previously reported fragile mutants, including hypo osmolarity sensitivity, temperature sensitivity and sensitivity to zymolyase indicating the existence of cell wall integrity defect. However, the three mutants differ from the reported mutants in some respects. Firstly, The temperature sensitivity of the isolated three mutants could not be complemented by osmotic stabilizer, while that of the PKC1 pathway mutants could be restored. Secondly, the degree of glycosylation of invertase in HF2, HF7 and HF9 did not decrease as that of srb1/vig9 mutants when compared with invertase in wild type strain respectively. In addition, the three mutants differ from each other. Hence, the three mutants are putative novel fragile mutants defect in cell wall. They can be used to further study the metabolism of yeast cell wall in biochemical and genetic levels and have the potential value to release intracellular heterologous proteins in high yield.展开更多
For 100 patients with gastric cancer, intraoperative intraperitoneal perfusion(IOIPP)using double-distilled water(D.D.W)with hibitane 37℃ for 5 minutes was performed.The anticancer effects were assessed cytologically...For 100 patients with gastric cancer, intraoperative intraperitoneal perfusion(IOIPP)using double-distilled water(D.D.W)with hibitane 37℃ for 5 minutes was performed.The anticancer effects were assessed cytologically in pre-IOIPP and post-IOPP specimens of the abdominal effusion and/or lavaged peritoneal fluid.The radical gastrectomy was performed in 65 of 93 cases with advanced gastric cancer, shed cancer cells in peritoneal cavity were detected in 16 of 65 cases with pre-IOIPP(24.62%)and to 3 of post-IOIPP cases(4.62%).There was significant difference between the two groups(P<0.01) . Shed cancer cells were inactivated in vivo estimated by the trypan-blue staining technique in 7 cases with radical surgery.But the effect of the IOIPP on shed cancer calls was not significant in cases with peritoneal metastasis. Also there were shed cancer cells in peritoneal cavity of patients with SS cancer histologically before the IOIPP(15. 56%)and iatrogenic diffusion of cancer cells during operation(7/24)in thisarticle.The above results indicated that the IOIPP with hypo-osmolar solution containing hibitane, 37℃ for 5 min was indispensable and useful for the killing of peritoneal shed cancer cells in order to prevent postoperative peritoneal recurrence in patients with radical surgery of advanced gastric cancer regardless of conditions of serosal invasion.展开更多
文摘AIM: To report the 3mo outcomes of collagen crosslinking(CXL) with a hypo-osmolar riboflavin in thin corneas with the thinnest thickness less than 400 μm without epithelium.METHODS: Eight eyes in 6 patients with age 26.2±4.8y were included in the study. All patients underwent CXL using a hypo-osmolar riboflavin solution after its de-epithelization. Best corrected visual acuity, manifest refraction, the thinnest corneal thickness, and endothelial cell density were evaluated before and 3mo after the procedure.RESULTS: The mean thinnest thickness of the cornea was 408.5 ±29.0 μm before treatment and reduced to369.8 ±24.8 μm after the removal of epithelium. With the application of the hypo-osmolar riboflavin solution, the thickness increased to 445.0 ±26.5 μm before CXL and recover to 412.5 ±22.7 μm at 3mo after treatment, P =0.659). Before surgery, the mean K-value of the apex of the keratoconus corneas was 57.6 ±4.0 diopters, and slightly decreased(54.7±4.9 diopters) after surgery(P =0.085). Mean best-corrected visual acuity was 0.55 ±0.23 logarithm of the minimal angle of resolution, and increased to 0.53±0.26 logarithm after surgery(P =0.879).The endothelial cell density was 2706.4 ±201.6 cells/mm2 before treatment, and slightly decreased( 2641. 2 ±218.2 cells/mm2) at last fellow up(P =0.002).CONCLUSION: Corneal collagen cross-linking with a hypo-osmolar riboflavin in thin corneas seems to be a promising treatment. Further study should be done to evaluate the safety and efficiency of CXL in thin corneas for the long-term.
文摘Saccharomyces cerevisiae osmotic fragile mutants were obtained from parental strain SH208 15 by N methyl N’ nitro N nitrosoguanidine(NTG) treatments. Three mutants, HF2, HF7 and HF9 showed high degree of release of the intracellular components into medium under hypo osmotic condition and were studied in details. They showed some similar phenotypes to the previously reported fragile mutants, including hypo osmolarity sensitivity, temperature sensitivity and sensitivity to zymolyase indicating the existence of cell wall integrity defect. However, the three mutants differ from the reported mutants in some respects. Firstly, The temperature sensitivity of the isolated three mutants could not be complemented by osmotic stabilizer, while that of the PKC1 pathway mutants could be restored. Secondly, the degree of glycosylation of invertase in HF2, HF7 and HF9 did not decrease as that of srb1/vig9 mutants when compared with invertase in wild type strain respectively. In addition, the three mutants differ from each other. Hence, the three mutants are putative novel fragile mutants defect in cell wall. They can be used to further study the metabolism of yeast cell wall in biochemical and genetic levels and have the potential value to release intracellular heterologous proteins in high yield.
文摘For 100 patients with gastric cancer, intraoperative intraperitoneal perfusion(IOIPP)using double-distilled water(D.D.W)with hibitane 37℃ for 5 minutes was performed.The anticancer effects were assessed cytologically in pre-IOIPP and post-IOPP specimens of the abdominal effusion and/or lavaged peritoneal fluid.The radical gastrectomy was performed in 65 of 93 cases with advanced gastric cancer, shed cancer cells in peritoneal cavity were detected in 16 of 65 cases with pre-IOIPP(24.62%)and to 3 of post-IOIPP cases(4.62%).There was significant difference between the two groups(P<0.01) . Shed cancer cells were inactivated in vivo estimated by the trypan-blue staining technique in 7 cases with radical surgery.But the effect of the IOIPP on shed cancer calls was not significant in cases with peritoneal metastasis. Also there were shed cancer cells in peritoneal cavity of patients with SS cancer histologically before the IOIPP(15. 56%)and iatrogenic diffusion of cancer cells during operation(7/24)in thisarticle.The above results indicated that the IOIPP with hypo-osmolar solution containing hibitane, 37℃ for 5 min was indispensable and useful for the killing of peritoneal shed cancer cells in order to prevent postoperative peritoneal recurrence in patients with radical surgery of advanced gastric cancer regardless of conditions of serosal invasion.
