Inhibiting effect of immunoeffector cells induced by denderitic cells vaccine on growth of PC3 and BEL7402

Objective:To compare effect of immunoprevention or immunotherapy based on denderitic cells (DCs),or supernatants on pancreatic carcinoma and hepatocelluar carcinoma in ritro and in vivo.Methods:DCs and monouclear cells (immunoeffecetor cells) were stimulated with hGM-CSF,hIL-4,hTNF-α,PC3 TA or BEL7402 TA and hIL-2,then DCs and immunoeffector cells were cocultured,and supernatants were harvested.In vitro,the immunoeffector cells were divided into A0 group (without DCs stimulated),A1 group (DCs stimulated,cultured with cytokines cocktail),A2 group (DCs stimulated,cultured with cytokines cocktail and tumor antigen.DCs vaccine).Cytoxicity assay was performed with lactate dehydogenase method,In vivo,the nude mice were allocated in 3 groups:prevention group,receiving immunoeffector cells activated by DCs vaccine 2 days before inoculation with PC3 or BEL7402;treatment group,receiving immunoeffector cells activated by DCs vaccine after development of implanted tumor in all nude mice;control group,receiving equivalents amount of RPMI1640 cultured liquid.On the 45th day,all the nude mice were sacrificed and the tumor was weighed.Results:The maximal inhibition rate of the A0.A1 andA2 were 3.5%.68.1%.81.0% in the BEL7402;4.5%,33.0%.62.4% in the PC3.The differences in tumor weight among three groups were significant,but the difference were not significant between the PC3 and BEL7402.Conclusions:DCs vaccine or supernatants may play an important role in treating and preventing against malignant tumor.

Changes in tumor-antigen expression profile as human small-cell lung cancers progress

Objective： Our group has previously observed that in patients with small-cell lung cancers （SCLCs）, the expression of a tumor antigen, glioma big potassium （gBK） ion channel, is higher at the time of death than when the cancer is first treated by surgical resection. This study aimed to determine whether this dichotomy was common in other potential lung tumor antigens by examining the same patient samples using our more extensive profile analysis of tumor-antigen precursor protein （TAPP）. We then tested the hypothesis that therapeutic intervention may inadvertently cause this increased gBK production. Methods： SCLC samples （eight surgical resections and three autopsy samples） and three control lungs were examined by quantitative real-time polymerase chain reaction for 42 potential TAPPs that represent potential T-cell-mediated immunological targets. Results： Twenty-two TAPP mRNAs displayed the same profile as gBK, i.e., more mRNAs were expressed at autopsy than in their surgical counterparts. B-cyclin and mouse double minute 2, human homolog of PS3-binding protein were elevated in both autopsy and surgical specimens above the normal-lung controls. When HTB119 cells were incubated with doxorubicin, gBK was strongly induced, as confirmed by intracellular flow cytometry with a gBK-specific antibody. Conclusion： Our findings suggested that more immunological targets became available as the tumor responded to chemotherapy and proceeded toward its terminal stages.