THE SECRETORY STIGMAS IN POPULUS: DEVELOPMENT, CYTOCHEMISTRY, ULTRASTRUCTURE AND THEIR RELATION TO INTERSECTIONAL INCOMPATIBILITY *

The stigmas of five species, Populus euphratica Oliv., P. alba L., P. simonii Carr., P. lasiocarpa Oliv. and P. nigra L. have been studied. Scanning electron microscopy re veals that exudates are present in the intercellular spaces, in the clefts between the multicel lular papillae and on the receptive surface. Release and movement of exudates can be visual ized when the fresh stigmas are stained with sudan Ⅲ and auramine O. Paraffin and semi thin resin sections of stigmas after glutaraldehyde osmium fixation evidence the lipidic nature of the exudates. Transmission electron microscopy reveals the glandular features of the stigmatic papillae cells, such as abundance of rough and smooth endoplasmic reticulum, polyribosomes, and well developed dictyosomes with secretory vesicles. Pellicle and epicuticular lamellate layers which have been considered as typical features of the dry type stigmas are also present in the species where stigmas appear extremely wet. It is concluded that stigmas in all of the five species are secretory at the receptive stage. Well developed generative and sperm cells were observed in the pollen tubes penetrating through the deep layers of the stigmatic tissue in the reciprocal crosses between P. euphratica and P. simonii, which indicated that there is no significant barrier in the stigma.

Genetic and Geographic Patterns of Duplicate DPL Genes Causing Genetic Incompatibility Within Rice:Implications for Multiple Domestication Events in Rice

Strong genetic incompatibilities exist between two primary rice subspecies,indica and japonica.However,the wild ancestors of rice,O.nivara Sharma et Shastry and O.rufipogon Griff.,are genetically compatible.How this genetic incompatibility became established has not been clearly elucidated.To provide insights into the process,we analyzed a pair of hybrid sterility genes in rice,DOPPELGANGER 1(DPL1)and DOPPELGANGER 2(DPL2).Either of the two loci can have one defective allele(DPL1-and DPL2-).Hybrid pollen carrying both DPL1-and DPL2-alleles is sterile.To explore the origination of DPL1-and DPL2-,we sequenced the DPL1 and DPL2 genes of 811 individual plants,including Oryza sativa(132),O.nivara(296)and O.rufipogon(383).We then obtained 20 DPL1 and 34 DPL2 sequences of O.sativa from online databases.Using these sequences,we analyzed the genetic and geographic distribution patterns of DPL genes in modern rice and its wild ancestors.Compared with the ancestral populations,DPL1-and DPL2-showed reduced diversity but increased frequency in modern rice.We speculated that the diversity reduction was due to a historic genetic bottleneck,and the frequency had likely increased because the defective alleles were preferred following this artificial selection.Such results indicated that standing variances in ancestral lines can lead to severe incompatibilities among descendants.Haplotype analysis indicated that the DPL1-haplotype of rice emerged from an O.nivara population in India,whereas the DPL2-haplotype emerged from O.rufipogon in South China.Hence,the evolutionary history of DPLs conforms to the presumed multiple domestication events of modern rice.

Sulfolane-Graphite Incompatibility and Its Mitigation in Li-ion Batteries

The non-flammability and high oxidation stability of sulfolane(SL)make it an excellent electrolyte candidate for lithium-ion batteries(LIBs).However,its incompatibility with graphitic anode prevents the realization of these advantages.To understand how this incompatibility arises on molecular level so that it can be suppressed,we combined theoretical calculation and experimental characterization and reveal that the primary Li^(+) solvation sheath in SL is depleted of fluorine source.Upon reduction,SL in such fluorine-poor solvation sheath generates insoluble dimer with poor electronic insulation,hence leading to slow but sustained parasitic reactions.When fluorine content in Li^(+)-SL solvation sheath is increased via salt concentration,a high stability LiF-rich interphase on graphite can be formed.This new understanding of the failure mechanism of graphite in SL-based electrolyte is of great significance in unlocking many possible electrolyte solvent candidates for the high-voltage cathode materials for next-generation LIBs.

ABO incompatibility in renal transplantation

ABO blood group incompatibility(ABO-I)was historically considered an absolute contraindication to kidney transplantation due to the significant risk of acute antibody-mediated rejection and early graft loss.Nevertheless,the urge to minimize the gap between the candidates’number on the waitlist for kidney transplants and the available kidney donors encourage investigation into finding ways to use organs from ABO-I kidney donors,especially in the era of using more potent immunosuppression therapies.This review aims to discuss a general overview of ABO-I kidney transplantation and the different protocols adopted by some transplant centers to meaningfully overcome this barrier.

Study on the cytoplasmic incompatibility in some natural population of Culex pipiens complex in China and Japan

The cytoplasmic incompatibility in the field population of Culex pipiens complex from China and Japan was studied. The results of the crossing experiments between 11 different strains suggest that some Japanese strains might be possible to be used for control Chinese strains by using male sterile technique, and the heavy infection of wolbachiae in mosquitoes may have some relations with the cytoplasmic incompatibility in the field population of Cx. pipiens complex.

Necrotic Incompatibility in Crosses of Bread Wheats in Argentina

Abstract： Synthetic wheats are the product of the cross between Triticum turgidum L. var. durum and T. tauschii. The 7＇. tauschii has shown excellent resistance to diseases, salinity, and drought, However, these synthetic wheats are also carriers of genes that produce varying degrees of necrosis, which is expressed as death of tissues in the hybrids （F l） generated from crosses with other bread wheats Necrotic incompatibility is a gradual premature leaf death in certain bread wheat F1 plants and it is caused by the interaction of two genes Nel and Ne2. In this paper 40 hybridizations with T. tauschii in the genetic constitution of some of the two parents made in 2006 and 2007 are presented, and their respective F1 planted at the CEI Barrow （Chacra Experimental de Barrow）. The plants that showed necrotic incompatibility had less growth than the normal F1 at tillering. Symptoms appeared at the beginning of tillering, remaining in that state and till jointing but they never headed. Out of the 16 Argentinean commercial cultivars evaluated, seven had the Ne allele in its genetic constitution and therefore showed necrotic incompatibility, whereas nine of them did not have the NE allele and their F 1 developed normally.

Anatomical Studies on Compatibility and Incompatibility of Some Solanaceous Plant Species to Field Dodder (<i>Cuscuta campestris</i>Yuncker)

Four solanaceous plant species, namely hot pepper (Capsicum frutescensL.), potato (Solanum tuberosumL.), tobacco (Nicotiana tabacumL.) and tomato (Lycopersicon esculentumMiller) were anatomically screened for compatibility/incompatibility to field dodder (Cuscuta campestrisYuncker, Cuscutaceae). The development of field dodder haustorium within stem tissues of the studied solanaceous plants was found to depend on the latter’s response to the former’s penetration. In hot pepper and tobacco, which showed a positive response, the haustorium was well developed and its searching hyphae established connections with their vascular tissues. However, potato and tomato showed a negative response and acquired different defense mechanisms against the parasitism of field dodder. In both of them, the field dodder haustorium and/or its searching hyphae exhibited distorted appearance and failed to establish connection with their vascular tissues. Thus, the tested solanaceous plant species could be classified as either compatible (hot pepper and tobacco) or incompatible (potato and tomato) hosts to field dodder.

Galaxy Evolution by the Incompatibility between Dark Matter and Baryonic Matter

The paper derives the galaxy evolution by the non-interacting (incompatibility) between dark matter and baryonic matter in terms of the short-range separation between dark matter and baryonic matter, so dark matter cannot contact baryonic matter. In the conventional CDM (cold dark matter) model, dark matter and baryonic matter are interactive (compatible), so dark matter can contact baryonic matter. However, the conventional CDM model fails to account for the failure to detect dark matter by the contact (interaction) between dark matter and baryonic matter, the shortage of small galaxies, the abundance of spiral galaxies, the old age of large galaxies, and the formation of thin spiral galaxies. The non-interacting (incompatible cold dark matter) model can account for these observed phenomena. The five periods of baryonic structure development in the order of increasing non-interacting (incompatibility) are the free baryonic matter, the baryonic droplet, the galaxy, the cluster, and the supercluster periods.

Experimental investigation of measurement incompatibility of mutually unbiased bases

Incompatible measurements are of fundamental importance to re-vealing the peculiar features of quantum theory,and are also use-ful resources in various quantum information tasks.In this work,we investigate the quantum incompatibility of mutually unbiased bases(MUBs)within the operational framework of quantum resource the-ory,and report an experimental validation via the task of state dis-crimination.In particular,we construct an experimentally friendly witness to detect incompatible MUBs,based on the probability of cor-rectly discriminating quantum states.Furthermore,we prove that the noise robustness of MUBs can be retrieved from violating the above witness.Finally,we experimentally test the incompatibility of MUBs of dimensionality ranging from 2 to 4,and demonstrate that it is more robust to noise,as either the dimensionality of measurements or the number of MUBs increases.Our results may aid the exploration of the essential roles of incompatible measurements in both theoretical and practical applications in quantum information.

Crossing experiments and behavioral observations reveal reproductive incompatibility among three putative species of the whitefly Bemisia tabaci

The whitefly Bemisia tabaci has a global distribution and extensive genetic diversity. Recent phylogenetic analyses as well as crossing experiments suggest that B. tabaci is a complex composed of 〉 20 cryptic species, but more crossing studies are required to examine the reproductive compatibility among the putative species and thus further clarify the systematics of this species complex. We conducted crossing experiments and behavioral observations to investigate the reproductive compatibility between the Mediterranean, Asia II 3, and Asia II 1 putative species ofB. tabaci collected from Zhejiang, China. Female progeny were never produced in inter-species crosses, demonstrating a lack of egg fertilization; while 55%-75% females were produced in all the intra-species treatments. Continuous behavioral observations showed that frequent courtship events occurred in both intra-species treatments and inter-putative species crosses. However, copulation events occurred only in the three intra-species treatments with one exception： that one copulation event occurred between Asia II 3 and Mediterranean in the crosses where two cohorts of females and males of different putative species were enclosed together in a small arena but were not allowed access to their intra-specific mates for a long period of time. These data demonstrated complete reproductive isolation between the Mediterranean, Asia II 3, and Asia II 1 putative species, and further showed that the isolation is due to lack of copulation. Demonstration of reproductive isolation between the Mediterranean and two indigenous putative species from China provides further evidence for the existence of cryptic species within the B. tabaci complex.

Incompatibility behavior of a symbiotic plasmid pMH7653Rb in Mesorhizobium huakuii 7653R

Mesorhizobium huakuii strain 7653R harbored two indigenous plasmids named pMH7653Ra and pMH7653Rb.The larger plasmid pMH7653Rb (symbiotic plasmid) was transferred to M.huakuii HN308SR harboring three plasmids: pMHHN308a,pMHHN308b and pMHHN308c,and HN3015SR harboring three plasmids: pMHHN3015a,pMHHN3015b and pMHHN3015c by tri-parent mating.Two stable indigenous plasmids,pMHHN308b and pMHHN308c of HN308SR,were co-eliminated due to the introduction of pMH7653Rb,and the transconjugant was named HN308SRN14.The results implied that pMH7653Rb and pMHHN308b,pMHHN308c were incompatible and might have been ascribed to the same incompatible group.The plasmid profiles of transconjugant HN3015SRN14 showed that the second largest plasmid pMHHN3015b of HN3015SR was cured due to the introduction of pMH7653Rb.The results also implied that pMH7653Rb and pMHHN3015b were incompatible.Results from plant nodulation tests showed that pMH7653Rb could only maintain the nodulation ability in transconjugant HN308SRN14 and its nodule number was more than that of wild strain HN308SR,but could not replace the nitrogen fixation effect of pMHHN308b and pMHHN308c.The plasmid cured mutant HN308SRN14D harboring only pMHHN308a formed null nodules that demonstrated pMHHN308a was relevant to nodulation ability.HN3015SRN14 harboring pMH7653Rb,pMHHN3015a and pMHHN3015c formed null nodules while HN3015SRN14D containing pMHHN3015a and pMHHN3015c lost the nodulation ability.The plasmid replication repC-like gene sequences were detected by a polymerase chain reaction from 7653R,HN308,HN3015,HN308SRN14 and HN3015SRN14.The repC gene sequence similarities of the strains tested attained 99%.

NDUFA8 potentially rescues Wolbachia-induced cytoplasmic incompatibility in Laodelphax striatellus

The endosymbiont Wolbachia manipulates host reproduction by several strategies, one of the most important of which is cytoplasmic incompatibility (CI). CI can be rescued when Wolbachia-infected males mate with females infected with the same Wolbachia strain. However, the potential rescue mechanism of CI in the small brown planthopper Laodelphax striatellus is unclear. In this study, comparative transcriptome analysis was applied to explore the effect of Wolbachia on L. striatellus eggs. A total of 1387 differentially expressed genes were identified. RNA interference of 7 Wolbachia-upregulated key planthopper genes reduced egg reproduction, suggesting that Wolbachia might improve fecundity in L. striatellus by affecting these 7 genes. Suppressing the expression of another upregulated gene, NDUFA8 (encoding NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 8-like) by RNA interference significantly increased the mortality of early embryos without affecting the number of deposited eggs. Wolbachia infection upregulated the mRNA level of NDUFA8, and dsNDUFA8 treatment of Wolbachia-infected females recreated CI-like symptoms, suggesting that NDUFA8 is associated with the rescue phenotype. Because all L. striatellus populations worldwide are infected with Wolbachia, NDUFA8 is a potential pest control target.

Numerical investigation of the effect of geosynthetic clay liner chemical incompatibility on flow and contaminant transport through a defective composite liner

A composite liner consisting of a geomembrane(GMB)and a geosynthetic clay liner(GCL)can be compromised by inorganic contaminants because of a defective GMB.When the composite liner with defective GMB is exposed to aggressive leachate conditions,the neglect of the chemical incompatibility of the GCL can potentially result in an underestimation of the leakage rate and flux through the composite liner.This paper proposed a numerical investigation on the effect of chemical incompatibility of GCL on the barrier performance of the composite liner with hole defect.Four cases with leachate solutions having varied cation valencies and ionic strengths were analyzed,in which the hydraulic conductivity of GCL was concentrationdependent.Both the effect of the chemical incompatibility of GCL and the mechanisms were analyzed.The incompatibility of GCL resulted in significant increases in leakage rate and flux through the composite liner by factors of up to 4.9 and 5.0,respectively.The incompatibility-affected area in GCL is located within 0.1 m from the center of the hole in the GMB.The coupled increase in the hydraulic conductivity of GCL and pore water concentration impacts the flux and leakage in a short period of time.With GCL chemical incompatibility considered,advection may dominate the contaminant transport through GCL.

IMPROVED HARMONIC INCOMPATIBILITY REMOVAL FOR SUSCEPTIBILITY MAPPING VIA REDUCTION OF BASIS MISMATCH

In quantitative susceptibility mapping(QSM),the background field removal is an essential data acquisition step because it has a significant effect on the restoration quality by generating a harmonic incompatibility in the measured local field data.Even though the sparsity based first generation harmonic incompatibility removal(1GHIRE)model has achieved the performance gain over the traditional approaches,the 1GHIRE model has to be further improved as there is a basis mismatch underlying in numerically solving Poisson’s equation for the background removal.In this paper,we propose the second generation harmonic incompatibility removal(2GHIRE)model to reduce a basis mismatch,inspired by the balanced approach in the tight frame based image restoration.Experimental results shows the superiority of the proposed 2GHIRE model both in the restoration qualities and the computational efficiency.

Toxicity-efficacy converting of ginseng combined with Fuzi Banxia incompatibility in heart failure stage of cor pulmonale

Objective：Fuzi Banxia is one of eighteen antagonisms,previous studies have shown that the incompatibility could play special effects in the specific condition of diseases and appropriate compatible environment.The present study aims to evaluate the toxicity-efficacy of ginseng combined with Fuzi Banxia incompatibility intervening in the heart failure stage of cor pulmonale and to explore its mechanism.Methods：Monocrotaline（MCT）-induced cor pulmonale were used in this study.Ultra high-resolution small animal ultrasound real-time imaging system and the right heart catheterization were used to estimate cardiac function.Semi automatic biochemical analyzer was used to test myocardial enzyme LDH,CK,and CK-MB in serum.The heart tissues were stained with HE,and TUNEL assay was used to assess the pathomorphological changes and myocardial apoptosis.The expression of hypertrophy and apoptosis associated genes：ANP,BNP,β-MHC,Bax,and Bcl-2 in the right ventricle were determined by RT-PCR.Results：Fuzi Banxia combined with ginseng obviously attenuated mortality,decreased RVHI,and increased cardiac index;RVSP and m PAP were significantly reduced,and EF and FS were raised obviously;Myocardial enzymes LDH,CK,and CK-MB were pronounced attenuated;heart diameter reduced,right ventricular dilatation was significantly decreased,inflammatory cell infiltration notably reduced,and cardiac apoptosis rate was decreased obviously.Meanwhile,the expression of hypertrophy-related ANP,BNP,andβ-MHC m RNA were up-regulated,the expression of apoptosis-related Bax m RNA was down-regulated,and the expression of anti-apoptosis-related Bcl-2 m RNA and Bcl-2/Bax ratio were upregulated.Conclusion：Ginseng compatible environment could attenuate cardiac toxicity of Fuzi Banxia incompatibility intervening in the heart failure stage of cor pulmonale,and improve cardiac function,which may be related to the expression of hypertrophy and apoptosis associated genes,and thus delay the occurrence and development of heart failure.

Twinning mediated anisotropic fracture behavior in bioimplant grade hot-rolled pure magnesium

Bioimplant grade hot-rolled magnesium with equiaxed microstructure and basal texture was examined for fracture toughness(FT)anisotropy using fatigue pre-cracked single-edge notch bending specimens with the notch,an||,⊥and 45°to rolling direction(RD).Due to adequate crack-tip plasticity,the size-independent elastic-plastic fracture toughness(JIC)were determined.Anisotropic JIC was ob-served due to different twin lamellae formation w.r.t.notch owing to the initial basal texture with{10¯10}and{11¯20}poles mostlyand⊥to RD.The out-of-plane tensile stresses activated the{10¯12}||10¯11||extension twins(ET)as usual with matrix-ET∑15b coincident site lattice boundary(CSLB)interfaces.While the in-plane tensile stress⊥to the crack-tip activated{10¯11}||10¯12||contraction twins(CT)that transform into{10¯11}-{10¯12}double twins(DT)with matrix-DT∑23b and∑15a CSLBs.For an||RD,large DT lamellae fraction formed at∼30°and few ETs at∼30°and∼90°to the notch with crack growth mainly via the∑23b/∑15a CSLB interfaces during FT.While,significant DT and ET lamellae developed at∼0°and∼60°with cracking via the matrix-DT∑23b/∑15a and matrix-ET||15b CSLBs for an⊥RD.The DT and ET lamellae activated at∼15°,and the crack propagated through∑15b for an∼45°to RD.The JIC and the crack-tip plastic zone decreases,while the elastic component of the J-integral(Jel)and the ET formation increases from an||,⊥,to∼45°to RD.The strain incompatibility of matrices was higher with the geometrically hard ETs than DTs.Thus,brittle interlamellar cracking occurred through the∑15b interfaces.In contrast,almost similar and higher crack-tip plasticity occurred in matrix and DT domains during crack propagation via||23b/||15a CSLBs.Crack growth through∑23b/||15a led to high JIC,both∑15b and||23b/||15a led to moderate JIC,and∑15b least JIC for an||,⊥and 45°to RD,respectively.

Tackling application limitations of high-safetyγ-butyrolactone electrolytes:Exploring mechanisms and proposing solutions

Developing wide-temperature and high-safety lithium-ion batteries(LIBs)presents significant challenges attributed to the absence of suitable solvents possessing broad liquid range and non-flammability properties.γ-Butyrolactone(GBL)has emerged as a promising solvent;however,its incompatibility with graphite anode has hindered its application.This limitation necessitates a comprehensive investigation into the underlying mechanisms and potential solutions.In this study,we achieve a molecular-level understanding of the perplexing interphase formation process by employing in-situ spectroelectrochemical techniques and density function calculations.Our findings reveal that,even at high salt concentrations,GBL consistently occupies the primary Li^(+)solvation sheath,leading to extensive GBL decomposition and the formation of a high-impedance and inorganic-poor solid-electrolyte interphase(SEI)layer.Contrary to manipulating solvation structures,our research demonstrates that the utilization of filmforming additives with higher reduction potential facilitates the pre-establishment of a robust SEI film on the graphite anode.This approach effectively inhibits GBL decomposition and significantly enhances the battery's lifespan.This study provides the first reported intrinsic understanding of the unique GBLgraphite incompatibility and offers valuable insights for the development of wide-temperature and high-safety LIBs.

The critical role of therapeutic plasma exchange in ABO-incompatible liver transplantation

Background:The shortage of donor liver restricts liver transplantation(LT).Nowadays,donor liver with ABO blood group incompatibility between donor and recipient has become an option to expand the source of donor liver.Although it is now possible to perform ABO-incompatible(ABO-I)LT,antibody-mediated rejection(AMR)has been recognized as the primary cause of desperate outcomes after ABO-I LT.Anti-A/B antibody is the trigger of immune response to ABO-I LT graft injury.Therapeutic plasma ex-change(TPE)can quickly reduce the titer of plasma antibodies and effectively inhibit humoral immunity.Data sources:We searched PubMed and CNKI databases using search terms“therapeutic plasma ex-change”,“ABO-incompatible liver transplantation”,“ABO-I LT”,“liver transplantation”,“LT”,“antibody-mediated rejection”,and“AMR”.Additional publications were identified by a manual search of references from key articles.The relevant publications published before September 30,2020 were included in this review.Results:Different centers have made different attempts on whether to use TPE,when to use TPE and how often to use TPE.However,the control standard of lectin revision level is always controversial,the target titer varies significantly from center to center,and the standard target titer has not yet been estab-lished.TPE has several schemes to reduce antibody titers,but there is a lack of clinical trials that provide standardized procedures.Conclusions:TPE is essential for ABO-I LT.Hence,further research and clinical trials should be conducted to determine the best regimen for TPE to remove ABO antibodies and prevent AMR.

Elucidating the interaction of kansui and licorice by comparative plasma/tissue metabolomics and a heatmap with relative fold change

Although compatibility is highly advocated in traditional Chinese medicine (TCM), inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects. Kansui and licorice, one of TCM “Eighteen Incompatible Medicaments”, are the most representative cases of improper herbal combination, which may still be applied simultaneously under given conditions. However, the potential mechanism of their compatibility and incompatibility is unclear. In the present study, two different ratios of kansui and licorice, representing their compatibility and incompatibility respectively, were designed to elucidate their interaction by comparative plasma/tissue metabolomics and a heatmap with relative fold change. As a result, glycocholic acid, prostaglandin F2a, dihydroceramide and sphinganine were screened out as the principal alternative biomarkers of compatibility group;sphinganine, dihydroceramide, arachidonic acid, leukotriene B4, acetoacetic acid and linoleic acid were those of incompatibility group. Based on the values of biomarkers in each tissue, the liver was identified as the compatible target organ, while the heart, liver, and kidney were the incompatible target organs. Furthermore, important pathways for compatibility and incompatibility were also constructed. These results help us to better understand and utilize the two herbs, and the study was the first to reveal some innate characters of herbs related to TCM “Eighteen Incompatible Medicaments”.

Outcomes of ABO-incompatible liver transplantation in end-stage liver disease patients co-infected with hepatitis B and human immunodeficiency virus

BACKGROUND Human immunodeficiency virus(HIV)-positive patients coinfected with hepatitis B virus(HBV)are eligible for liver transplantation(LT)in Africa and Southeast Asia,particularly China.However,the outcome of HIV-HBV coinfected patients referred for ABO-incompatible LT(ABOi-LT)is unknown.AIM To clarify the outcome of ABOi-LT for HIV-HBV coinfected patients with endstage liver disease(ESLD).METHODS We report on two Chinese HIV-HBV coinfected patients with ESLD who underwent A to O brain-dead donor LT and reviewed the literature on HIV-HBV coinfected patients treated with ABO-compatible LT.The pretransplantation HIV viral load was undetectable,with no active opportunistic infections.Induction therapy consisted of two sessions of plasmapheresis and a single dose of rituximab in two split doses,followed by an intraoperative regimen of intravenous immunoglobulin,methylprednisolone,and basiliximab.Post-transplant maintenance immunosuppressive agents consisted of tacrolimus and mycophenolate mofetil,and prednisone.RESULTS At the intermediate-term follow-up,patients showed undetectable HIV viral load,CD4(+)T cell counts greater than 150 cells/μL,no HBV recurrence,and stable liver function.A liver allograft biopsy showed no evidence of acute cellular rejection.Both patients survived at 36-42 mo of follow-up.CONCLUSION This is the first report of ABOi-LT in HIV-HBV recipients with good intermediate-term outcomes,suggesting that ABOi-LT may be feasible and safe for HIV-HBV coinfected patients with ESLD.