Development and Age Structure of Ramets of Kalimeris integrifolia Populations in the Songnen Plains, Northeast China

Kalimeris integrifolia Turcz. is a root sucker form perennial herb of Compositae. In die late period of growing season, fresh fleshy radicels grow up above the soil surface to develop root-borne buds, which in turn grow into ramets and taproot. On the basis of observation on numerous samples, methods to classify the age of ramets and buds for the root sucker herbaceous plant population and to estimate the ages of taproots were established according to their morphology. The age of ramets was classified based on the regeneration times of taproot buds. Ramets developed from new root sucker buds were classified as 1 a. After 1 a ramets died, the ramets reproduced from the taproots were 2 a, and so on. New buds from a root sucker were 0 a buds, other age classes of buds were consistent with that of ramets, i.e. buds produced from taproots of I a ramets were 1 a buds, and so on. In the hay clipping meadows of the Songnen Plains, for K. integrifolia population, there are three age classes of ramets, representing a steady age structure. The maximal age of taproots of ramets is 4 a. Among all age classes, the population productivity of 2 a ramets was the highest, and that of I a ramets was the lowest. The ramet productivity increased with ramet age. During the early period of growing season, the productivity of 3 a ramets was the highest, but after the middle of growing season, I a ramets showed the greatest relative growth rate. The population size and age structure in the next year could be accurately forecasted by the number of fresh roots with budlet and present population in late autumn. The potential population in the bud banks was more than two times of the present population, which may provide sufficient substitution in the future.

Structural Characterization and Retention Time Prediction for Components of Essential Oil of Meconopsis Integrifolia Flowers

A molecular structural characterization （MSC） method called reduced molecular electronegativity-distance vector （MEDVR） was used to describe the molecular structures of 55 components of meconopsis integrifolia flowers. By use of stepwise multiple regression （SMR） and partial least square （PLS） methods, a model with the correlation coefficient （R1） of 0.987 and the standard deviation （SD1） of 1.377 could be obtained. Then through multiple linear regression （MLR）, another model with the correlation coefficient （R2） of 0.989 and standard deviation （SD2） of 1.395 could be constructed. Furthermore, in virtue of variable screening by the stepwise multiple regression technique （SMR）, 8 vectors were selected to build up another model with its correlation coefficient （R3） and standard deviation （SD3） of 0.989 and 1.366, respectively. Then all the three models were evaluated by performing cross-validation with the leave-one-out （LOO） procedure, and the correlation coefficients （QCV） were 0.981, 0.976 and 0.979, respectively. The results show that the models constructed could provide estimation stability and favorable predictive ability.

Annual Variation of Mineral Nutrition Elements Content in Macadamia integrifolia

[Objective] To investigate the annual variation of 9 mineral nutrition ele-ments content in Macadamia integrifolia leaves. [Method] Twenty 6-year-old ＆quot;Guire No. 1＆quot; healthy plants were selected. On the 15th of every month in 2011, 1 leaf in the second round from top-branch in 4 directions of the 20 trees was col ected. The content of 9 mineral elements of N, P, K, Ca, Mg, Zn, Cu, Fe and Mn were deter-mined. [Result] The results showed that the N, P, K content in leaves reached a peak in April, then N content decreased slowly, P content stayed stable, and K con-tent increased slightly; Ca, Fe content decreased in April to different degrees and then increased slowly. Mg content was consistent al year round, meanwhile Mn content decreased slightly. Cu and Zn content reached a peak in April, and then presented a rise-fal trend. [Conclusion] N, K, Ca, Mg should be supplemented in time in practice.

Anti-atherosclerotic activity of root bark of Premna integrifolia Linn.in high fat diet induced atherosclerosis model rats

Premna integrifolia Linn. is a medicinal plant used in ＂Dhasamula＂ drug preparation of Ayurvedic systems of medicine in the treatment of various ailments like bronchitis, dyspepsia, liver disorders, piles, constipation,hyperlipidemia and fever. The anti-atherosclerotic activity of hydroalcoholic extract（HAE） of root bark of P.integrifolia was evaluated in high fat diet induced atherosclerosis rats. Sixty Wistar rats were divided into six groups： the first group served as control, the second group was fed with high fat diet and the other three groups were fed with high fat diet along with various concentrations of HAE and the last group was treated with atorvastatin for 30 days. Lipid and lipoprotein profile, atherogenic index, and cardiac markers and histopathological evaluation of aorta were determined in high fat diet induced atherosclerosis rats. HAE of P.integrifolia produced a significant and dose-dependent anti-atherosclerotic activity in terms of reduction in lipids and lipoprotein profile, atherogenic index, HMG-Co A reductase activity, marker enzymes such as lactate dehydrogenase（LDH）, creatine phosphokinase（CPK）, aspartate transaminase（AST）, alanine transaminase（ALT） and alkaline phosphatase（ALP）, alteration in collagen and calcium contents, mild mineralization and focal rupture of intima and media of aorta was noticed in treated groups as compared to the control. The results suggested that anti-atherosclerotic activity of HAE of P. integrifolia Linn. was due to its modulatory activity on metabolic pathway of lipid. The results contribute to the validation of the traditional use of Agnimantha in high fat diet induced atherosclerosis rats.

Chromosomal-level genome of macadamia(Macadamia integrifolia)

Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research.

Pharmacognostic evaluation of leaf and root bark of Holoptelea integrifolia Roxb.

Objective:To evaluate the pharmacognostic characters of an important medicinal plant, Holoptelcu integrifolia(H.integrifolia) Roxb.Methods:The pharmacognostic studies were carried out in terms of organoleptic,microscopic,macroscopic and fluorescence analysis.Results: The characteristic microscopic features of leaves were observed as trichomes,multicellular trichomes,xylem cells,phloem cells,collenchyma,vascular bundles,spongy parenchyma and palisade cells.The characteristic microscopic features of root bark included cork cambium, primary cortex,phloem fibers,medullary rays,endodermis,pericycle and lignified fibers in the transverse section and longitudinal section.The characteristic microscopy of root bark powder showed the presence of cortex cells,sieve tubes,calcium oxalate crystals and lignified fibers. Macroscopic study showed that leaf shape-oval,apex-acute,base-cordate and leaf margin was entire with glabrous surface,bitter taste and characteristic odour.The morphological features of root bark showed deep fissured,rough and firm surface with rhitydome and the periderm parallel to cambium.Conclusions:Various pharmacognostic characters observed in this study help in the identification and standardization of H.integrifolia.

In vitro anthelminthic efficacy of Dichrocephala integrifolia(Asteraceae)extracts on the gastro-intestinal nematode parasite of mice:Heligmosomoides bakeri(Nematoda,Heligmosomatidae)

Objective:To evaluate the ovicidal and larvicidal activities of aqueous and ethanolic extracts of leaves of Dichrocephala integrifolia(D.integrifolia)against the eggs(fresh and embryonnated),the first and second larval stages of Heligmosomoides bakeri.In order to verify if this medicinal plant possesses active compounds capable of inhibiting the embryonation and hatching of eggs or to induce the mortality of larvae(L1 and L2).Methods:Dried extracts were diluted in distilled water to obtain five different concentrations:625,1 250,2500,3750 and 5000μg/mL.Fresh eggs obtained from artificially infected mice feces were exposed to these different concentrations for 48 h.Time of contact for embryonated eggs was 6 h while L1 and L2 larvae were exposed for 24 h.Distilled water(placebo)and 1.5%DMSO were used as negative controls.Results:Distilled water,and1.5%DMSO had no effect on embryonation,hatching and larval survival.Aqueous extracts of D.integrifolia showed a weak activity against all stages of the parasite at all concentrations tested.On the contrary,the ethanolic extract of D.integrifolia inhibited the embryonation of 87.5%of fresh eggs,the hatching of 81.1%of embryonated eggs and induced the mortality of 98.1%and 98%of L1 and L2 larvae respectively at 5000μg/mL.Conclusions:The results of the present study indicate that the ethanolic extracts of D.integrifolia contained compounds with ovicidal and larvicidal properties.In spite of these results,in vivo tests,studies on toxicity and mechanism of action of active compounds are also needed to validate the utilisation of this medicinal plant by population of Dschang-Cameroon to treat gastro-intestinal parasites.

Studies of Microstructure of Kernels of Macadamia integrifolia and Its Hybrids through MRI, X-Ray Tomography and Confocal Microscopy

The aim of this study was to apply the existing techniques that enable examination ofmacadamia kernels to provide a better understanding of physico-chemical properties of kernels during postharvest processing. These techniques, such as X-ray tomography, could be applied for quality monitoring in the macadamia industry. The objectives of this study were to investigate the browning centre symptoms that usually occur in macadamia nuts-in-shell. The applied techniques included confocal microscopy, X-ray tomography and magnetic resonance imaging （MRI）. Five different varieties of macadamia nuts （A38, 246, 816, 842 and Daddow） were selected to include distinct characteristics, such as drop pattern and growing location. Analysis of the microstructure of kernels by confocal microscopy showed the distribution of possible brown pigment compounds as well as the distribution of lipids, carbohydrates and proteins inside macadamia cells. Physical properties data, including shell density and seed to volume ratio, were obtained by X-ray tomography. Magnetic resonance diffusion tensor imaging used in this study showed marked differences in microstructure which indicate that different varieties exhibit different microstructures expressed as fraction ofanisotropy and apparent diffusion coefficient that appear to be related to the occurrence of the brown centre. Hence, the findings of this study have potential to improve the existing postharvest techniques used in the macadamia processing industry. They will be of benefit to the industry in terms of improved quality control and cost reduction.

Antioxidant activity of dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats

Objective: Infectious diseases such as typhoid fever lead to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work evaluated the antioxidant activity and histopathological effects of the dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats.Methods: The S. typhi-infected rats concurrently received daily doses of D. integrifolia extract at doses of 25, 50 and 100 mg/kg body weight or ciprofloxacin(5 mg/kg body weight) for 15 days. Body temperature was measured daily during infection and treatment periods. At the end of treatment period, the animals were sacrificed and biological responses including hematological parameters, superoxide dismutase and catalase activities, and glutathione, malondialdehyde and nitric oxide concentrations were evaluated.Results: The elevated body temperature induced by infection was significantly decreased in animals treated with 25, 50 or 100 mg/kg of the extract. Platelet levels decreased slightly in infected rats, while treatment with the dichloromethane fraction of D. integrifolia significantly increased platelet levels;this response was greater than that elicited by ciprofloxacin. The doses of 50 and 100 mg/kg of the dichloromethane fraction of D. integrifolia notably decreased monocyte and neutrophil values. Activity of superoxide dismutase and catalase and levels of glutathione in the tissues of treated animals were increased significantly(P < 0.01), while malondialdehyde and nitric oxide levels were significantly decreased(P < 0.01), following treatment with the dichloromethane fraction of D. integrifolia.Conclusion: The results of this study show that the dichloromethane fraction of D. integrifolia has protective effects against a series of pathological conditions initiated by oxidation and tissue damage in the course of a S. typhi infection.

全缘叶绿绒蒿(Meconopsis integrifolia)的ISSR遗传多样性分析

为研究全缘叶绿绒蒿的ISSR遗传多样性,本研究以全缘叶绿绒蒿16个居群共185个个体为研究对象,采用简单序列重复区间扩增多态性(ISSR)标记技术分析全缘叶绿绒蒿遗传多样性,利用Popgen1.32计算遗传相似系数(GS)、Shannon指数(I)、Nei’s指数(He)等遗传参数;AMOVA进行遗传变异分析;利用MEGA4.0软件及UPGMA法进行聚类分析并构建树状图,并用MVSP中的主成分分析(PCA)对群体的遗传关系进行聚类分析;用TFPGA软件进行Mental检测。ISSR的21条引物共扩增出279个条带,平均每条引物扩增出13.3个条带,其中多态性条带278个,条带在150~2 000 bp之间均有分布,物种水平上的遗传多样性百分比PPB=99.64%,Nei’s基因多样性指数(He)范围在0.052 0~0.208 3之间,Shannon’s多样性信息指数(I)在0.079 5~0.313 4范围内;遗传分化系数(Gst)和AMOVA分析均显示遗传变异主要存在于种群间。种群间基因流(Nm)小于1,遗传漂变在全缘叶绿绒蒿的遗传分化中所起作用较大。Mental相关性检验,p值均大于0.05,说明遗传距离与地理距离间相关性不显著,地理距离对居群间的遗传分化没有明显影响。通过全缘叶绿绒蒿的遗传多样性研究发现,ISSR是一种非常便捷、有效的分子标记技术,能够为后续的种质鉴定、杂交育种和资源收集与合理开发利用提供一定的理论依据和参考。

不同干燥方式对柳蒿芽山野菜挥发性风味的影响研究

为研究不同干燥方式对柳蒿芽山野菜中的挥发性风味化合物影响的差异性,采用气相色谱-离子迁移谱联用(GC-IMS)技术对3种不同干燥处理的柳蒿芽样品进行挥发性化合物检测分析。从样品中共检出103种挥发性有机化合物(volatile organic compounds,VOCs);经归一化处理,结合指纹图谱分析,真空冷冻干燥样品中93种VOCs得到了有效的保留,显著高于电热鼓风干燥和自然干燥组,根据气味描述数据库检索可知冷冻干燥对柳蒿芽中的甜香、焦香气味有较好的保留。正交偏最小二乘法判别分析(OPLS-DA)结果筛选出了12种对区分不同干燥方式的柳蒿芽挥发性风味物质贡献较大的变量,分别为十二醛、2-甲基异冰片、1-辛醇、薄荷脑、(E,E)-2,4-庚二烯醛、乙酸冰片酯、香茅醛、DL-2-羟基-4-甲基戊酸乙酯、1-石竹烯、三亚乙基二胺。

云南云县澳洲坚果种植存在的问题及对策初探

通过对云南省临沧市云县澳洲坚果生产的调查,分析了云县澳洲坚果种植方面主要制约因素,其中果园内良种率低是制约澳洲坚果产量的首要因素,其次是科学抚育管理,针对该主要问题,云县林草相关部门采用高接换种为主的低产园改造技术措施在生产中进行实施,取得良好的效果。

澳洲坚果抗氧化肽的分离纯化及肽段鉴定

为研究澳洲坚果抗氧化肽的抗氧化活性和氨基酸组成,以复配蛋白酶水解澳洲坚果粕制备粗多肽,利用超滤、大孔树脂纯化技术制备了抗氧化活性最佳的分子量小于1000 Da的多肽,采用Sephadex G-15凝胶对其分离并评价各组分对DPPH、羟基、ABTS+自由基的清除能力与还原能力,筛选出抗氧化活性最强组分,利用液相色谱-串联质谱技术(liquid chromatography and tandem mass spectrometry,LC-MS/MS)进行鉴定并分析。结果表明,葡聚糖凝胶柱层析分离出G1、G2、G3组分,其中G3具有最佳的抗氧化活性,其羟基自由基清除能力半抑制浓度(half maximal inhibitory concentration,IC_(50))6.18 mg/mL与还原能力IC_(50)2.19 mg/mL优于谷胱甘肽,DPPH自由基清除能力IC_(50)0.50 mg/mL,ABTS+自由基清除能力IC_(50)0.02 mg/mL;通过液相色谱-串联质谱鉴定G3含有46个肽段,肽段长度均小于10个氨基酸,匹配得分大于200分的9条肽段分子量为631~920 Da,均无毒性;筛选获得高抗氧化活性肽HLLPK、KEFFP、KEFFPA,其分子量分别为606.84、666.83、737.92 Da。本研究初步揭示了澳洲坚果抗氧化肽组成,深化了澳洲坚果抗氧化肽的研究,为澳洲坚果抗氧化肽的开发利用提供了理论参考。

全缘铁线莲种子休眠特性及破眠方法研究

目的:全缘铁线莲(Clematis integrifolia L.)是铁线莲属直立多年生草本或亚灌木,有很高的观赏价值,了解种子休眠特性及破眠方法可为其引种驯化和园林应用提供科学依据。方法:以全缘铁线莲种子为研究材料,通过纸培法探究控制条件下种子休眠及萌发的生物学特性。结果:果实形态成熟时种子尚不具备发芽能力,种子生活力为86.33%±2.87%,种皮(包括瘦果果皮)透水性良好,种胚无吸水障碍。种子浸取液对小白菜种子发芽及幼苗生长有显著抑制作用。果实形态成熟时种胚发育尚不完全,低温层积能促进种胚发育。低温层积90 d可将种子发芽率提高到73.33%±4.71%。使用200 mg·L-1赤霉素浸种24 h可使种子发芽率提高到61.11%±5.67%。结论:全缘铁线莲种子休眠是由于萌发抑制物和种胚分化不完全而导致的形态生理休眠,播种前将种子低温层积90 d或使用200 mg·L-1赤霉素浸种24 h可以有效打破种子的休眠从而促进种子萌发。

澳洲坚果加工与产业副产物综合利用研究进展

我国是世界澳洲坚果种植大国,但我国澳洲坚果还处在初加工阶段,加工副产物也未被充分利用,为促进我国澳洲坚果的精深加工利用,综述了澳洲坚果的营养成分、加工产品、产业副产物的综合利用现状,并对澳洲坚果的综合加工利用前景进行了展望。澳洲坚果富含脂肪、蛋白质、矿质元素等营养成分,其主要加工产品有坚果油和开口坚果;澳洲坚果产业副产物包括饼粕、青皮、果壳、叶、花,在提取蛋白质,制作堆肥、燃料、茶叶、花茶等方面有应用价值。未来我国应重视澳洲坚果精深加工,充分利用澳洲坚果副产物,提升澳洲坚果产品的附加值。

柳蒿芽多糖的制备及抗氧化活性研究

目的:从柳蒿芽中分离、纯化多糖,对其抗氧化活性进行研究。方法:采用响应面法优化超声波辅助酶法提取柳蒿芽多糖的工艺条件。结果:最佳提取工艺为:超声功率300 W,料液比1∶30 g/mL,超声时间46 min,纤维素酶添加量2.0%,果胶酶添加量3.0%,此条件下多糖得率为7.67%。采用DEAE-52层析柱分离、纯化共得到6个多糖组分。对AIP-2和AIP-3两个组分进行抗氧化活性的研究表明,不同质量浓度AIP-2和AIP-3处理对H_(2)O_(2)诱导的HepG2细胞氧化损伤均有较强的浓度依赖性保护作用。与模型组相比,高质量浓度AIP-2和AIP-3(50μg/mL)处理使细胞存活率提高了54.35%和60.26%,SOD活力提高了35.86%和38.98%,CAT活力提高了173.02%和205.42%,GSH-PX活力提高了50.24%和50.78%,MDA含量降低了46.13%和53.87%。结论:柳蒿芽多糖具有良好的抗氧化活性。

石漠化地区澳洲坚果果实品质及产量的综合评价

【目的】了解石漠化地区澳洲坚果种质资源的品质特征,筛选出能适应于石漠化地区种植的优良澳洲坚果品种。【方法】以贵州石漠化地区的13个澳洲坚果品种为研究对象,分析测定澳洲坚果果实产量、外观品质、营养成分和脂肪酸组分等34项指标,采用变异分析、相关性分析及主成分分析法,对果实品质与产量进行分析和综合评价。【结果】不同澳洲坚果品种果实外观品质和产量存在明显差异,澳洲坚果单壳果质量为6.42~12.37 g,以Nanya No.12和HAES344的单壳果质量最大;出种率为35.10%~55.37%,以HAES344的出种率最高并显著高于其他品种;果仁质量为2.27~4.36 g,出仁率为29.76%~46.44%,单株产量为1.66~21.04 kg,其中Nanya No.116的果仁质量、出仁率和单株产量显著高于其他品种。不同品种澳洲坚果营养品质指标变异均较丰富,变异系数为0.98%~41.82%,其中Fe含量的变异系数最大,为41.82%,蛋白质和粗脂肪含量变异系数相对较小,分别为3.84%和0.98%;果仁脂肪酸组分中,十五烷酸和十七烷酸的变异系数最大,均为14.29%,最小的是棕榈酸、油酸,分别为5.53%和2.86%,且果仁脂肪酸组分中均以油酸、棕榈油酸为主。澳洲坚果果实主要营养品质指标间有明显相关性,经主成分分析将12项主要营养品质指标综合为4个主成分,其累积贡献率可达82.828%。各品种主成分的综合得分由高到低依次为:Nanya No.3、Own Choice、Hinde、HAES788、Nanya No.l、Nanya No.2、HAES344、Nanya No.12、Guire No.1、Nanya No.116、HVA16、HVA4、HAES695。【结论】Nanya No.3、Own Choice的果实综合性状表现均相对较好,这两个品种可通过深入研究作为贵州石漠化地区推广种植的优良品种。此外,Nanya No.l、Nanya No.12、Nanya No.116等品种的产量和营养品质均较好,可作为主要良种进行推广。

基于最大熵模型的气候变化情景下澳洲坚果潜在适宜生境研究

为了研究气候变化情景下澳洲坚果在云南省的潜在适宜生境,采用当前和未来2050年RCP45气候变化情景下的19个生物气候因子及最大熵模型MaxEnt进行澳洲坚果生境模型构建,并进行适宜生境等级划分及空间变化特点分析。结果表明,2050年RCP45气候变化情景下3个等级的适宜生境大体上仍然保持与当前相似的空间分布格局,即高度适宜生境主要分布在云南西南部和南部,中、低度适宜生境分布在高度适宜生境区以北及以东区域。未来气候变化引起高度和中度适宜生境面积小幅度缩减(5.6%和2.4%),低度适宜生境面积增加22.5%。气候变化同时引起高度适宜生境景观格局破碎化。未来气候变化引起的澳洲坚果在云南高、中度适宜种植区总面积略有缩减,虽幅度不大,但空间分布上发生位移,且呈现破碎化趋势,产业规划时应考虑产业生命周期内气候变化造成对适宜生境迁移的影响。

不同处理对2种绿绒蒿种子萌发特性的影响

对比不同GA_(3)浓度、GA_(3)不同浸泡时间、不同温度和不同光照处理对全缘叶绿绒蒿和总状绿绒蒿种子萌发的影响,寻找出最有利于这两种绿绒蒿种子萌发的方法。结果表明:(1)100 mg·L^(-1)GA_(3)处理最能促进全缘叶绿绒蒿种子萌发,总状绿绒蒿用300 mg·L^(-1) GA_(3)处理最优,其次为200 mg·L^(-1) GA_(3)处理。(2)而12 h处理和18 h处理为不同GA_(3)处理时间对总状绿绒蒿种子萌发中最优的两个处理;全缘叶绿绒蒿种子GA_(3)三个处理均优于对照。(3)25℃处理与25℃/15℃处理最能促进全缘叶绿绒蒿种子萌发,25℃处理最能促进总状绿绒蒿种子萌发。(4)12 h光照/12 h黑暗交替处理均最能促进全缘叶绿绒蒿种子和总状绿绒蒿种子萌发。

基于WGCNA鉴定全缘叶绿绒蒿类黄酮合成途径关键基因

【目的】黄酮类化合物具有抗炎、抗癌、抑菌等多种功效,是全缘叶绿绒蒿的主要药用成分之一。通过分析全缘叶绿绒蒿不同部位的空间代谢组与转录组信息,挖掘调控黄酮类化合物合成的关键基因,为研究全缘叶绿绒蒿类黄酮合成机制提供理论参考,并为提高类黄酮含量遗传育种奠定分子基础。【方法】以全缘叶绿绒蒿的根、茎、叶和花瓣为材料,对不同部位进行转录组测序,并通过空间代谢组数据分析黄酮类化合物在不同部位中的分布情况,利用加权基因共表达网络分析(WGCNA)鉴定出与黄酮类化合物合成密切相关的关键模块和关键基因。另外,挑选12个基因进行qRT-PCR,验证转录组数据的可靠性。【结果】全缘叶绿绒蒿中黄酮类化合物在不同部位呈现差异积累,花瓣是黄酮类化合物积累的主要部位,同时明确了8种主要黄酮类化合物。转录组测序共获得20085个表达基因,仅在花中表达的基因有286个,是仅在其他部位中表达基因数量的3.6—4.2倍。利用WGCNA对过滤后的高表达差异基因进行划分,共获得14个共表达模块,确定了关键模块MEturquoise和MEgreen与8个主要黄酮类化合物显著相关(P<0.05)。KEGG分析发现这两个模块基因主要在代谢相关的通路中富集,在黄酮类化合物合成相关通路上也有基因富集,两个模块分别包含了18个和6个与黄酮类化合物合成相关的基因,并从模块中筛选到14个核心结构基因(5个CHS、2个HIDH、2个CCoAOMT以及FLS、CYP75B1、CHI、HCT和CYP73A)和1个转录因子HB2,这些基因多在花瓣或是茎中高表达。qRT-PCR所测基因表达变化趋势与转录组基本一致,表明利用该转录组数据所得出的分析结果可信。【结论】全缘叶绿绒蒿黄酮类化合物积累和基因表达在不同器官间具有显著差异,联合分析筛选到与黄酮类化合物积累密切相关的14个核心结构基因和1个转录因子,这些基因可能在调控全缘叶绿绒蒿不同器官黄酮类化合物的合成和差异积累过程中起到关键作用。