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UV-B irradiation enhances the accumulation of beneficial glucosinolates induced by melatonin in Chinese kale sprout
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作者 Mengyu Wang Jiansheng Wang +6 位作者 Yuhe Yang Zhiqing Li Ghazala Mustafa Yubo Li Lihong Liu Zuofa Zhang Qiaomei Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期995-1006,共12页
Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by appl... Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts. 展开更多
关键词 MELATONIN Chinese kale SPROUTS UV-B GLUCOSINOLATES Nutritional quality Second metabolism
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基于Kales概念模型的护理干预对痴呆患者激越行为的影响
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作者 杜红娣 王薇 +1 位作者 何志娟 薄琳 《现代临床护理》 2024年第1期49-55,共7页
目的 探讨基于Kales概念模型的护理干预对痴呆患者激越行为的影响。方法 选取2021年12月至2022年12月在北京市某三级甲等综合医院老年科住院的80例痴呆患者作为研究对象。采用随机数字表法将患者分为对照组和观察组。对照组(n=40)接受... 目的 探讨基于Kales概念模型的护理干预对痴呆患者激越行为的影响。方法 选取2021年12月至2022年12月在北京市某三级甲等综合医院老年科住院的80例痴呆患者作为研究对象。采用随机数字表法将患者分为对照组和观察组。对照组(n=40)接受常规护理,观察组(n=40)在常规护理的基础上实施基于Kales概念模型的护理干预,分别比较干预前、干预2w后、干预4w后两组患者激越行为及生活质量。结果 重复测量方差分析显示,两组患者干预3个时间点激越行为得分和生活质量得分的时间效应、组间效应和交互效应比较,差异具有统计学意义(均P<0.05)。干预后观察组患者激越行为得分均低于对照组,两组比较,差异具有统计学意义(P<0.001)。干预后观察组患者生活质量得分均高于对照组,两组比较,差异有统计学意义(P<0.01)。结论 基于Kales概念模型的护理干预有助于改善痴呆患者激越行为,提高痴呆患者生活质量。 展开更多
关键词 kales概念模型 痴呆 精神行为症状 激越行为
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Effect of Different Irrigation Maximums on Accumulation and Distribution of Material in Chinese Kale 被引量:3
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作者 曹健 张白鸽 +1 位作者 罗少波 宋钊 《Agricultural Science & Technology》 CAS 2016年第3期629-632,共4页
[Objective] The aim was to explore effects of soil moisture on dry matter accumulation and distribution and growth of kai-lan. [Method] In the context of the same irrigation frequency, the influence of irrigation maxi... [Objective] The aim was to explore effects of soil moisture on dry matter accumulation and distribution and growth of kai-lan. [Method] In the context of the same irrigation frequency, the influence of irrigation maximum was researched on growth rate, net assimilation rate, and dry matter distribution of kai-lan. [Result] The results showed that regulation on soil moisture extremely significantly affected accumulation of photosynthate. When soil moisture was insufficient, the growth term from seedling to mature extended and growth rate declined sharply. Besides, the growth term was of extremely significant negative correlation with growth rate. Meanwhile,leaf assimilation rate decreased considerably. For example, when soil moisture was65%, distribution of dry matter in plant organs tended to be rational. [Conclusion]Deficit irrigation would significantly lower growth rate, and improve dry matter distribution in organs and economic yield and quality of kai-lan. 展开更多
关键词 Chinese kale Soil Moisture Photosynthate accumulation IRRIGATION
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Effect of Different Irrigation Maximums on Absorption and Utilization of Water in Chinese Kale 被引量:3
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作者 曹健 张白鸽 +1 位作者 宋钊 罗少波 《Agricultural Science & Technology》 CAS 2016年第2期344-346,408,共4页
[Objective] This study was conducted to investigate the effect of soil moisture on the growth and water utilization of Chinese kale. [Method] The law of water absorption and utilization of Chinese kale was researched ... [Objective] This study was conducted to investigate the effect of soil moisture on the growth and water utilization of Chinese kale. [Method] The law of water absorption and utilization of Chinese kale was researched under the same irri- gation frequency and different irrigation maximums. [Result] Soil water deficit re- duced water contents in plants in seedling stage and in plants and various organs in maturation stage; water consumption of individual plant was the lowest in 55% treatment of soil A and 45% treatment of soil B, which showed the values of 2.244 and 2.235 L/plant, respectively, and saved water by 23.91% and 21.14% compared with CK; water use efficiencies of soil A with a water content of 55% and soil B with a water content of 35% were the highest, i.e., 6.043 and 5.958 g/L, which were higher than that of CK by 20.09% and 41.72%, respectively; and regulated deficit irrigation also improved irrigation water productivity, and the two kinds of soil both showed in 75% treatments the highest irrigation water productivities, i.e., 40.44 and 40.49 g/L, which were higher than that of CK by 5.64% and 13.39%, respec- tively. [Conclusion[ Controlling irrigation maximum could save water consumed by Chinese kale, improve water use efficiency and improve yield and quality. 展开更多
关键词 Chinese kale Soil water IRRIGATION
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Monosomic Addition Lines of Flowering Chinese Cabbage(B.campestris L.ssp.chinensis var.parachinensis L.H.Bailey)-Chinese Kale(B.oleracea L.var.alboglabra L.H.Bailey) 被引量:6
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作者 WANG Xin-e ZHANG Cheng-he XUAN Shu-xin MAN Hong LIU Hai-he SHEN Shu-xing 《Agricultural Sciences in China》 CAS CSCD 2008年第6期656-663,共8页
Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition li... Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid (AAC, 2n = 3x = 29) was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 (tetraploid, AAAA, 2n = 4x = 40) x B. oleracea var. alboglabra Baihua 9705 (diploid, CC, 2n = 2x = 18) by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines (AA + C2, AA + C3, AA + C4, AA + C6, AA + C7) were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents 展开更多
关键词 flowering Chinese cabbage Chinese kale ALLOTRIPLOID alien addition lines
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Kale Juice Improves Coronary Artery Disease Risk Factors in Hypercholesterolemic Men 被引量:1
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作者 SOO YEON KIM SUN YOON +2 位作者 SOO MI KWON KYE SOOK PARK YANG CHA LEE-KIM 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2008年第2期91-97,共7页
Objective To evaluate the effect of 3-month kale (Brassica oleracea acephala) juice supplementation on coronary artery disease risk factors among hypercholesterolemic men. Methods Thirty-two men with hypercholestero... Objective To evaluate the effect of 3-month kale (Brassica oleracea acephala) juice supplementation on coronary artery disease risk factors among hypercholesterolemic men. Methods Thirty-two men with hypercholesterolemia (〉 200 mg/dL) were recruited after annual health examinations among the faculty and staff at university. The subjects consumed 150 mL of kale juice per day for a 12-week intervention period. Dietary and anthropometric assessments were performed and blood samples were collected to evaluate biochemical profiles before and after supplementation. Results Serum concentrations of HDL-cholesterol, and HDL- to LDL-cholesterol ratio were significantly increased by 27% (P〈0.0001) and 52% (P〈0.0001), respectively. The LDL-cholesterol concentration and the atherogenic index were significantly reduced by 10% (P=0.0007) and 24.2% (P〈0.0001), respectively without affecting body mass index, waist and hip circumferences, or nutrient intakes after three months of supplementation. While there was no difference in the concentration of malondialdehyde, significant increase in glutathione peroxidase activity (P=0.0005) were accompanied by a significant increase in the serum selenium level (P=0.0132). It was also found that the responses of these risk factors to kale juice administration were dependent on smoking status. Conclusion Regular meals supplementation with kale juice can favorably influence serum lipid profiles and antioxidant systems, and hence contribute to reduce the risks of coronary artery disease in male subjects with hyperlipidemia. 展开更多
关键词 kale juice HYPERLIPIDEMIA Coronary artery disease Lipid profile Antioxidant system
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Effect of Ammonium- and Potassium-Loaded Zeolite on Kale (<i>Brassica alboglabra</i>) Growth and Soil Property 被引量:1
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作者 Junxi Li Chido Wee Bokyoon Sohn 《American Journal of Plant Sciences》 2013年第10期1976-1982,共7页
We investigated the feasibility of using ammonium- and potassium-loaded zeolite (NK-Z) as carriers for fertilizer and for slow release of nitrogen (N) and potassium (K). Plant growth response and soil analysis were pe... We investigated the feasibility of using ammonium- and potassium-loaded zeolite (NK-Z) as carriers for fertilizer and for slow release of nitrogen (N) and potassium (K). Plant growth response and soil analysis were performed. The results indicated an increase in the total harvest weight of kale (Brassica alboglabra Bailey). Furthermore, higher levels of N and K were detected in soil applied with NK-Z than in soil applied with compound fertilizers. The leaf fresh weight of kale in the fertilizer treatments including NK-Z were 2118.4 and 2111.3 g·plant-1, while the leaf fresh weights of kale in the treatment without NK-Z was 2018.0 g·plant-1. The N and K contents in the soils were maintained in the high level in the NK-Z treatment compared that in control treatment. The results indicate that NK-Z has a great potential as the slow-release fertilizer reducing pollution by preventing leaching to the ground water. 展开更多
关键词 ZEOLITE Slow-Release Fertilizer Soil Property kale GROWTH Response
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<i>In vitro</i>Bile Acid Binding of Mustard Greens, Kale, Broccoli, Cabbage and Green Bell Pepper Improves with Sautéing Compared with Raw or Other Methods of Preparation 被引量:1
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作者 Talwinder S. Kahlon Rebecca R. Milczarek Mei-Chin M. Chiu 《Food and Nutrition Sciences》 2012年第7期951-958,共8页
Bile acid binding potential of foods and food fractions has been related to lowering the risk of heart disease and that of cancer. Steam cooking has been observed to significantly improve bile acid binding of green/le... Bile acid binding potential of foods and food fractions has been related to lowering the risk of heart disease and that of cancer. Steam cooking has been observed to significantly improve bile acid binding of green/leafy vegetables. It was hypothesized that other cooking methods could further improve the bile acid binding of various vegetables. Sautée cooking resulted in in vitro bile acid binding measured on a dry matter basis relative to cholestyramine of 14% for mustard greens and kale, 9% for broccoli, 8% for collard greens, 6% for cabbage, and 5% for green bell pepper. These results point to the significantly different (P ≤ 0.05) health promoting potential of mustard greens = kale > broccoli > collard greens > cabbage > green bell pepper. Sautéing significantly improved in vitro bile acid binding of mustard greens, kale, broccoli, cabbage and green bell pepper compared with steaming, boiling or raw (uncooked). Collard greens exhibited significantly higher bile acid binding by steaming compared with sautéing, boiling or raw. Data suggest that the cooking method with most heath promoting potential for mustard greens, kale, broccoli, cabbage and green bell pepper should be sautéing. Steaming should be used for collard greens as the cooking method. These green/leafy vegetables, when consumed regularly after sautéing, would promote a healthy lifestyle and have the potential to lower the risk of premature degenerative diseases. 展开更多
关键词 In vitro Bile Acid Binding Mustard Greens kale BROCCOLI CABBAGE Green Bell Pepper and Collard Greens Sautéing STEAMING Boiling
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Evaluation of Levels of Selected Heavy Metals in Kales, Soils and Water Collected from Irrigated Farms along River Moiben, Uasin-Gishu County, Kenya 被引量:1
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作者 Teresa Akenga Kiplagat Ayabei +2 位作者 Emmy Kerich Vincent Sudoi Cyrus Kuya 《Journal of Geoscience and Environment Protection》 2020年第2期144-155,共12页
There has been a rapidly increasing urbanization and industrialization as well as increased usage of agrochemicals in the recent few years which have resulted in accumulation of heavy metals in cultivated food, soils ... There has been a rapidly increasing urbanization and industrialization as well as increased usage of agrochemicals in the recent few years which have resulted in accumulation of heavy metals in cultivated food, soils and water. This research aimed at establishing the levels of Zn2+, Cd2+, Cu2+, Cr2+, Mn2+, Fe2+ and Pb2+ metal ions in kales, soil and irrigation water on farms along river Moiben. Twenty seven samples of vegetables, soil and water samples were collected using purposive sampling method, that is, the samples were collected from the households who had kales in their farms. Samples were then dried, grounded, digested and analyzed using Inductive Couple Plasma-Optical Emission Spectroscopy (ICP-OES). The results showed that the Fe had the highest mean in soil and water with the values of 250.22 ± 85.37 and 0.72 ± 0.33 mg/kg respectively, while in kales Zn value was highest with a value of 0.0154 ± 0.007 mg/kg. The metal ion concentrations in the soils and the irrigation water were higher than in kales. The concentrations on the metal ions were following this order Fe > Mn > Zn > Cu > Cr > Pb > Cd for soil as well as for water but for the kales sample it followed slightly different order Zn > Fe > Mn > Cu > Cr > Pb > Cd. In soil samples, metal ions concentrations (mg/kg) were found to be high compared to the levels in water and kales. ANOVA tests revealed that the mean difference in heavy metals concentration from different stations within the area was insignificant (p > 0.05) with an exception of Cd (p = 0.001) in water samples, Fe (p = 0.007) in kales samples, Zn (p = 0.016) and Cd (p = 0.011) in the samples of soil. Results were compared to the acceptable levels set by World Health Organization (WHO) and the study showed that for kales, concentrations of the metal ions were all lower than the (WHO) set standards. For water samples, Fe, Pb, Mn metal ions were above the WHO set standards. The presence of the investigated heavy metals in the samples could be pointed to excessive use of agrochemicals as indicated by our earlier survey on the use of agrochemicals. We therefore recommend thorough investigations and monitoring of the said heavy metals in the commercially distributed agrochemicals. 展开更多
关键词 HEAVY Metals kales SOILS and WATER IRRIGATED Farms
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The Agronomic Techniques as Determinants of the Phenolic Content and the Biological Antioxidant Effect of Palm-Tree Kale
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作者 Francesca Danesi Veronica Valli +1 位作者 Simona Elementi Luigi Filippo D’Antuono 《Food and Nutrition Sciences》 2014年第1期1-7,共7页
The content of phytochemicals in vegetables is strongly affected by genetic, agronomic, and environmental factors, and by transportation and storage conditions, potentially affecting the sensory and putative health-pr... The content of phytochemicals in vegetables is strongly affected by genetic, agronomic, and environmental factors, and by transportation and storage conditions, potentially affecting the sensory and putative health-promoting properties. In this study the impact of different agricultural techniques on the phenolics content and antioxidant activity of extracts from a traditional Italian kale landrace, called palm-tree kale (Brassica oleracea L., ssp. acephala DC, var. sabellica L.) was evaluated. Furthermore, the antioxidant effects of the different extracts were assessed in a biological system using primary cultures of neonatal rat cardiomyocytes. The final aim was to evidence whether and how the agronomic practices can affect the antioxidant properties of kale not only in vitro but also in cells. Results herein reported underline the influence of the crop production strategies in establishing the potential health benefits of a vegetable. This research approach could be useful for the selection of production techniques that are able to provide edible vegetables with higher functional activity, and evidences the need to study the food domain as a whole with the nutrition domain, and to integrate all results in order to have an overall “foodomics” vision allowing the improvement of health and well-being. 展开更多
关键词 kale Brassica OLERACEA L. Cultured CARDIOMYOCYTES Antioxidants PHYTOCHEMICALS AGRONOMIC TECHNIQUES Plant Density Nitrogen Fertilization
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Whole Grain Gluten-Free High Protein Vegetable Snacks of Buckwheat Peanut Meal and Kale
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作者 Talwinder S. Kahlon Roberto J. Avena-Bustillos Mei-Chen M. Chiu 《Food and Nutrition Sciences》 2018年第4期335-345,共11页
Whole grain gluten-free high protein vegetable snacks were evaluated. The snacks were Buckwheat-Peanut Meal-Kale (BPK), BPK-Garlic, BPK-Onion and BPK-Ginger. Peanut meal was utilized to increase the protein content of... Whole grain gluten-free high protein vegetable snacks were evaluated. The snacks were Buckwheat-Peanut Meal-Kale (BPK), BPK-Garlic, BPK-Onion and BPK-Ginger. Peanut meal was utilized to increase the protein content of these snacks as well as to add value to this agricultural byproduct. Snack dough was prepared using water nearly 1:1 as other as is ingredients. About 20 g of snack dough was placed on preheated KrumKake Express 839 Baker and cooked for 2 minutes. Sixty-nine in-house volunteers judged Color/Appearance and Texture/Mouth feel to be similar for the snacks tested. Taste/Flavor for BPK, BPK-Garlic and BPK-Onion snacks was similar and significantly higher (p ≤ 0.05) than for BPK-Ginger snacks. Odor/Aroma for BPK-Garlic snacks was significantly higher than for BPK-Ginger snacks. Acceptance for BPK and BPK-Garlic snacks was significantly higher than for BPK-Ginger snacks. Ex-pansion 3.6 - 4.2, porosity 0.72 - 0.75 and water activity 0.35 - 0.38 suggests that the snacks tested were light, crispy and have good anti-microbial stability. Acceptance was BPK and BPK-Garlic 94%, BPK-Onion 86% and BPK-Ginger 78%. These snacks contained only 3 - 4 ingredients and could be made in any house hold kitchen and/or in commercial production. Acceptance of 78% - 94% is very desirable. These whole grain gluten-free high protein vegetable healthy nutritious tasty snacks offer choice for all consumers, including individual’s sensitive to gluten. 展开更多
关键词 Sensory Evaluation BUCKWHEAT kale PEANUT MEAL GLUTEN-FREE Water Activity
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Polyphenol Composition and Antioxidant Activity of Different Potentially Functional Kale-Based Snacks
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作者 Filomena Nazzaro Federica Cardinale +2 位作者 Autilia Cozzolino Tiziana Granese Florinda Fratianni 《Food and Nutrition Sciences》 2014年第12期1145-1152,共8页
Four types of kale-based snacks were investigated on the basis of polyphenol content and composition, glucosinolates amount, and antioxidant activity. The amount of polyphenols resulted to be higher than that of raw m... Four types of kale-based snacks were investigated on the basis of polyphenol content and composition, glucosinolates amount, and antioxidant activity. The amount of polyphenols resulted to be higher than that of raw material, fresh kale (0.57 ± 0.03 mg GAE). The antioxidant activity turned out indeed very effective, resulting sufficient just from 0.72 mg to 2.1 mg to dishearten the activity of 1 ml DPPH to 50%. Glucosinolates present in kale-based snacks ranged from 1.17 mg/gr to 1.52 mg of sinigrin equivalent/gr. Polyphenol profile, obtained by UPLC-DAD analysis allowed us to identify gallic acid, chlorogenic acid, and catechin common in all samples. Other polyphenols, that is caffeic acid, p-coumaric acid, and ferulic acid, were found in almost all samples, almost all more abundant than fresh kale, representing a precious source of beneficial metabolites. Our results suggest that such snacks could represent an effective functional dietary supplement also if compared to fresh vegetable. Manufacturing of kale-based snacks does not negatively affect the beneficial effect of the raw material;indeed, such snacks could positively meet people’s expectation, mainly children and young, well known consumers of snacks, which, also in such manner, could be addressed towards a healthier dietary regime. 展开更多
关键词 kale Antioxidant POLYPHENOLS GLUCOSINOLATES UPLC
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Construction of Plant Antisense Expression Vector with Defective in Anther Dehiscence1 Gene Fragment of Chinese Kale
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作者 Yaoguo Qin Jianjun Lei +3 位作者 Cuiqin Yang Yongli Zhai Bihao Cao Guoju Chen 《Journal of Life Sciences》 2011年第6期416-420,共5页
A pair of primers was designed according to the reported conserved sequence of the defective in anther dehiscencel (DAD1) gene ofArabidopsis thaliana and Brassica rapa. A 558 bp long fragment was amplified from geno... A pair of primers was designed according to the reported conserved sequence of the defective in anther dehiscencel (DAD1) gene ofArabidopsis thaliana and Brassica rapa. A 558 bp long fragment was amplified from genomic DNA of Chinese kale, showing more than 88% identity with the known DAD1 nucleotide sequence and no intron. The reverse of the amplified fragment was ligated to the downstream of the CaMV35S promoter in the plant expression vector pBIl21. Antisense expression vector pBII21-DAD1F was constructed with DAD1 fragment of Chinese kale, and was transferred into Agrobacterium tumefaciens, which will be used in the transformation to create male sterile materials of Chinese kale. 展开更多
关键词 Chinese kale Brassica oleracea var. alboglabra DAD1 antisense expression vector.
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Flavonoids and ROS Play Opposing Roles in Mediating Pollination in Ornamental Kale (Brassica oleracea var. acephala) 被引量:8
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作者 Xingguo Lan Jia Yang +4 位作者 Kumar Abhinandan Yuzhe Nie Xiaoyu Li Yuhua Li Marcus A. Samuel 《Molecular Plant》 SCIE CAS CSCD 2017年第10期1361-1364,共4页
Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible (genetically similar) pollen from germination and fertilizing the ovule, leading to e... Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible (genetically similar) pollen from germination and fertilizing the ovule, leading to either defective or genetically unfavorable embryos. In Brassicaceae, self-incompatibility (SI) is a genetic mechanism in the stigmas that can discern compatible versus incompatible mate (pollen), in turn, rejecting self or incompatible pollen, while allowing non-self or compatible pollen to develop successfully to fertilize the ovules. Thus, stigmas have evolved complex mechanisms and specialized proteins to reject incompatible mates and to recognize and accept compatible pollen (Doucet et al., 2016). The fact that SI has been shown to exclusively operate in the stigmas temporally before and during anthesis (Kandasamy et al., 1993) indicates that multiple metabolic pathways essential for pollination exist in these stigmatic papillary cells. 展开更多
关键词 FLAVONOIDS ROS Play Opposing Roles Mediating Pollination Ornamental kale Brassica oleracea var acephala
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Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale(Brassica oleracea var.alboglabra Bailey) 被引量:10
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作者 SUN Bo ZHANG Fen +6 位作者 XUE Shengling CHANG Jiaqi ZHENG Aihong JIANG Min MIAO Huiying WANG Qiaomei TANG Haoru 《Horticultural Plant Journal》 SCIE 2018年第3期94-102,共9页
ζ-Carotene desaturase(ZDS)is an important enzyme in carotenoid biosynthesis.Here,the Brassica oleracea var.alboglabra ZDS(Boa ZDS)gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction(... ζ-Carotene desaturase(ZDS)is an important enzyme in carotenoid biosynthesis.Here,the Brassica oleracea var.alboglabra ZDS(Boa ZDS)gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction(RT-PCR)and deposited in Gen Bank(accession number KY662297).The Boa ZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein.Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B.oleracea var.capitata and B.rapa.The promoter sequence of the Boa ZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses.Semiquantitative RT-PCR analysis showed that Boa ZDS expression varied among different developmental stages and organs.Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage.The leaves showed the highest ZDS expression levels compared to the other organs.ZDS expression decreased in all flower tissues during blooming.The fused protein of Boa ZDS was obtained by prokaryotic expression.Heterologous expression of Boa ZDS in Escherichia coli confirmed that Boa ZDS encodes a functionalζ-carotene desaturase that increases β-carotene accumulation in E.coli cells harboring a β-carotene-producing plasmid.The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. 展开更多
关键词 Chinese kale Brassica oleracea var alboglabra Bailey carotenoids ζ-carotene desaturase(ZDS) CLONING gene expression prokaryotic expression heterologous expression
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Germplasm Diversity of Chinese Kale in China 被引量:12
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作者 LEI Jianjun CHEN Guoju +1 位作者 CHEN Changming CAO Bihao 《Horticultural Plant Journal》 SCIE 2017年第3期101-104,共4页
Chinese kale is an important vegetable crop in China, especially in South China where the majority of varieties are grown. It originated in South China and spread throughout Southeast Asia thereafter. Chinese kale can... Chinese kale is an important vegetable crop in China, especially in South China where the majority of varieties are grown. It originated in South China and spread throughout Southeast Asia thereafter. Chinese kale can be classified into two types according to whether the petals are white or yellow. There are also three main cultivated types based on the edible organs, including the stalk and leaf type, the stalk type,and the leaf type. Two primary types have also been defined based on stalk color, including green stalks and red stalks. They are also cultivated based on the type of stalk, including main stalk and lateral stalk types. Significant differences have also been observed in glucosinolate content among the varieties, and a 40-fold difference in neoindle-3-methyl glucosinolate was detected across the cultivars. 展开更多
关键词 Chinese kale Brassica alboglabra Bailey DIVERSITY
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Effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of Chinese kale sprouts 被引量:4
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作者 Hui-ying MIAO Meng-yu WANG +3 位作者 Jia-qi CHANG Han TAO Bo SUN Qiao-mei WANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2017年第12期1093-1100,共8页
Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid(GA_3). The combination of 3%(0.0... Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid(GA_3). The combination of 3%(0.03 g/ml) glucose and 5 μmol/L GA_3 treatment was effective in increasing glucosinolate content while glucose or GA_3 treatment alone did not influence significantly almost all individual glucosinolates or total glucosinolates. The total phenolic content and antioxidant activity of Chinese kale sprouts were enhanced by combined treatment with glucose and GA_3, which could be useful in improving the main health-promoting compounds and antioxidant activity in Chinese kale sprouts. 展开更多
关键词 GLUCOSINOLATE ANTIOXIDANT GLUCOSE Gibberellic acid Chinese kale sprouts
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Application of plasma-activated water for Escherichia coli decontamination and shelf-life extension of kale 被引量:1
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作者 Hexiang Wang Yingxia Li +7 位作者 Qian Xi Rongwei Han Patrick J.Cullen Qijing Du Yongxin Yang Fereidoun Forghani Jiacheng Zhang Jun Wang 《Food Quality and Safety》 SCIE CSCD 2022年第3期416-426,共11页
The aim of this study was to examine the effectiveness of plasma-activated water(PAW)for inactivating Escherichia coli(E.col)and retention of key quality factors for kale.Different plasma discharge times(1,2,3,4,5,and... The aim of this study was to examine the effectiveness of plasma-activated water(PAW)for inactivating Escherichia coli(E.col)and retention of key quality factors for kale.Different plasma discharge times(1,2,3,4,5,and 10 min)and different exposure times(2,4,6,8,10,and 15 min)were used to investigate the inactivation effect of E.coli spot-inoculated on kale.The influence of different exposure times on the pH,hardness and color of kale was studied post-treatment.In addition,the effects of PAW on the shelf-life of kale over 12 d of storage at 4℃ were investigated.The results showed that after the treatment of 5-PAW-8(8 min treatment by PAW generated by 5 min plasma discharge),the population of E.coli on kale was reduced by approximately 1.55 log colony-forming units(CFU)/g and the changes in pH,hardness and color of kale were not significant(P>0.05).During the storage period after 5-PAW-8 treatment,the hardness,weight loss,color,surface morphology and ascorbic acid were found to be better than those of the two control groups(P<0.05).Furthermore,no significant changes were observed in pH values,the content of total phenols,or 1,1-dipheny1-2-picrylhydrazyl radical scavenging capacity(P>0.05).It is indicated that PAW treatment is a promising methodfor improving microbiological safety and extending the shelf-life of kale. 展开更多
关键词 Plasma-activated water kale Escherichia coli shelf-life quality
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芥蓝新种质创制及其在育种上的应用
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作者 张德双 辛晓云 +7 位作者 张凤兰 余阳俊 于拴仓 苏同兵 赵岫云 汪维红 李佩荣 卢桂香 《中国蔬菜》 北大核心 2024年第3期90-96,共7页
采用常规杂交方法,以芥蓝为母本,以甘蓝种的不同变种为父本进行杂交,从F2中选择优良单株,经回交和多代连续自交,最终创制出紫色性状(肉质茎、叶片、叶脉、花蕾)、蜡粉性状(无蜡粉肉质茎、无蜡粉叶片)、肉质茎形状(短纺锤形、柱形)、侧... 采用常规杂交方法,以芥蓝为母本,以甘蓝种的不同变种为父本进行杂交,从F2中选择优良单株,经回交和多代连续自交,最终创制出紫色性状(肉质茎、叶片、叶脉、花蕾)、蜡粉性状(无蜡粉肉质茎、无蜡粉叶片)、肉质茎形状(短纺锤形、柱形)、侧薹性状(早发、多发、颜色紫色或绿色)以及花瓣颜色(浅黄色)等不同类型的芥蓝新种质。应用这些材料成功选育出京紫1号、京紫2号、京紫3号、21M2芥蓝和22M4芥蓝等芥蓝新品种。研究结果为芥蓝种质资源创制提供了科学依据,为芥蓝新品种选育奠定了基础。 展开更多
关键词 芥蓝 种质 创制 一代杂种
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高效氯氰菊酯和吡丙醚在芥蓝中的残留消解及膳食暴露风险评估
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作者 唐红霞 李玉博 +4 位作者 张颂函 吴竞 董茂锋 王伟民 赵莉 《植物保护》 CAS CSCD 北大核心 2024年第1期232-238,271,共8页
为明确高效氯氰菊酯和吡丙醚在芥蓝上施用后的残留行为和膳食暴露风险,研究基于规范田间残留试验、目标农药在芥蓝上残留分析方法,得到芥蓝中2种农药的残留水平,结合我国膳食结构不同性别/年龄组食物消费量及体重数据评估了高效氯氰菊... 为明确高效氯氰菊酯和吡丙醚在芥蓝上施用后的残留行为和膳食暴露风险,研究基于规范田间残留试验、目标农药在芥蓝上残留分析方法,得到芥蓝中2种农药的残留水平,结合我国膳食结构不同性别/年龄组食物消费量及体重数据评估了高效氯氰菊酯和吡丙醚对各类消费人群的长期和短期膳食摄入风险。结果表明:高效氯氰菊酯和吡丙醚分别在0.01~1.0 mg/L和0.005~1.0 mg/L范围内线性关系良好,决定系数(R2)≥0.996 7。在3个添加水平下,芥蓝中2种农药的平均回收率为70.6%~113.4%,相对标准偏差(RSD)为0.5%~8.5%,定量限均为0.01 mg/kg。10%高氯·吡丙醚微乳剂以推荐高剂量施药,高效氯氰菊酯和吡丙醚在芥蓝中的消解符合一级动力学,半衰期分别为3.9~10.1 d和4.8~6.3 d。最终残留试验结果表明,最后一次施药3、5、7、10 d后,芥蓝中高效氯氰菊酯的最终残留量≤0.904 mg/kg,吡丙醚的最终残留量≤0.202 mg/kg。膳食风险评估表明,我国不同人群的长期慢性暴露风险最大值为27.26%;短期急性暴露风险最大值为67.17%,表明对不同年龄段、不同性别人群健康不会产生不可接受的风险。 展开更多
关键词 芥蓝 高效氯氰菊酯 吡丙醚 膳食摄入评估
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