Objective:To find the key targets of muscle atrophy after spinal cord injury(SCI)were excavated,to construct the lncRNA-miRNA-mRNA regulatory network based on bioinformatics analysis,and to verify the expression chang...Objective:To find the key targets of muscle atrophy after spinal cord injury(SCI)were excavated,to construct the lncRNA-miRNA-mRNA regulatory network based on bioinformatics analysis,and to verify the expression changes of key regulatory networks in muscle atrophy after SCI by animal experiments,so as to seek new research directions for the pathogenesis and treatment of muscle atrophy after SCI.Methods:The GSE21497 data set was downloaded from the GEO database for differential expression gene screening and WGCNA treatment.Combined with the online prediction database,key mRNAs were screened out.GO and KEGG enrichment analyses of key mRNAs were performed using the DAVID database to construct the lncRNA-miRNA-mRNA regulatory network.The key regulatory genes were selected and then verified by RT-qPCR.Results:A total of 1405 differentially expressed genes were screened,and 30 key mRNAs were predicted by the WGCNA and online database.GO and KEGG enrichment analyses showed that it was mainly enriched in the functions of neuron regeneration,protection,signal transmission,the HIF signaling pathway,PD-L1 expression and the PD-1 checkpoint pathway.Four key regulatory networks were identified(LINC00410/miR-17-5p/KCNK10,LINC00410/miR-17-5p/PCDHA3,LINC00410/miR-20b-5p/KCNK10,LINC00410/miR-20b-5p/PCDHA3).The results of RT-qPCR showed that,compared with the control group,the expression of miR-17-5p and miR-20b-5p in the observation group increased,and the expression of KCNK10 and PCDHA3 decreased.Conclusions:MiR-17-5p,miR-20b-5p,KCNK10,and PCDHA3 may play an important regulatory role in the regeneration,protection,and signal transmission of neurons,which is expected to become a new target for the diagnosis and treatment of muscle atrophy after SCI.展开更多
三阴性乳腺癌(triple-negative breast cancer,TNBC)是具有侵袭性的一种乳腺癌亚型,其特征是复发率和转移率较高。非编码RNA特别是长链非编码RNA(lncRNA)和microRNAs(miRNA)是人类基因转录组的主要组成部分,能与调节健康和疾病的编码转...三阴性乳腺癌(triple-negative breast cancer,TNBC)是具有侵袭性的一种乳腺癌亚型,其特征是复发率和转移率较高。非编码RNA特别是长链非编码RNA(lncRNA)和microRNAs(miRNA)是人类基因转录组的主要组成部分,能与调节健康和疾病的编码转录本相互作用。近年来,研究证实,lncRNA、miRNA和mRNA之间复杂的调控网络在TNBC发生发展的不同方面起着重要作用,但其潜在的分子机制尚未阐明。该文主要综述了lncRNA、miRNA及其下游mRNA之间调控关系的最新研究进展,并强调了lncRNA-miRNA-mRNA网络在TNBC发生发展及诊疗中的潜在作用,旨在为了解TNBC的发病机制和开发新的诊断与治疗策略提供重要线索。展开更多
基金National Natural Science Foundation of China(No.81960417)Guangxi Natural Science Foundation Project(No.2018GXNSFAA050033)+1 种基金Guangxi Science and Technology Key R&D Project(No.Guike AB20159027)Guangxi Natural Science Foundation Youth Fund Project(No.2022GXNSFBA035545)。
文摘Objective:To find the key targets of muscle atrophy after spinal cord injury(SCI)were excavated,to construct the lncRNA-miRNA-mRNA regulatory network based on bioinformatics analysis,and to verify the expression changes of key regulatory networks in muscle atrophy after SCI by animal experiments,so as to seek new research directions for the pathogenesis and treatment of muscle atrophy after SCI.Methods:The GSE21497 data set was downloaded from the GEO database for differential expression gene screening and WGCNA treatment.Combined with the online prediction database,key mRNAs were screened out.GO and KEGG enrichment analyses of key mRNAs were performed using the DAVID database to construct the lncRNA-miRNA-mRNA regulatory network.The key regulatory genes were selected and then verified by RT-qPCR.Results:A total of 1405 differentially expressed genes were screened,and 30 key mRNAs were predicted by the WGCNA and online database.GO and KEGG enrichment analyses showed that it was mainly enriched in the functions of neuron regeneration,protection,signal transmission,the HIF signaling pathway,PD-L1 expression and the PD-1 checkpoint pathway.Four key regulatory networks were identified(LINC00410/miR-17-5p/KCNK10,LINC00410/miR-17-5p/PCDHA3,LINC00410/miR-20b-5p/KCNK10,LINC00410/miR-20b-5p/PCDHA3).The results of RT-qPCR showed that,compared with the control group,the expression of miR-17-5p and miR-20b-5p in the observation group increased,and the expression of KCNK10 and PCDHA3 decreased.Conclusions:MiR-17-5p,miR-20b-5p,KCNK10,and PCDHA3 may play an important regulatory role in the regeneration,protection,and signal transmission of neurons,which is expected to become a new target for the diagnosis and treatment of muscle atrophy after SCI.
文摘三阴性乳腺癌(triple-negative breast cancer,TNBC)是具有侵袭性的一种乳腺癌亚型,其特征是复发率和转移率较高。非编码RNA特别是长链非编码RNA(lncRNA)和microRNAs(miRNA)是人类基因转录组的主要组成部分,能与调节健康和疾病的编码转录本相互作用。近年来,研究证实,lncRNA、miRNA和mRNA之间复杂的调控网络在TNBC发生发展的不同方面起着重要作用,但其潜在的分子机制尚未阐明。该文主要综述了lncRNA、miRNA及其下游mRNA之间调控关系的最新研究进展,并强调了lncRNA-miRNA-mRNA网络在TNBC发生发展及诊疗中的潜在作用,旨在为了解TNBC的发病机制和开发新的诊断与治疗策略提供重要线索。
