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A Multi-Objective Optimization for Locating Maintenance Stations and Operator Dispatching of Corrective Maintenance
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作者 Chao-Lung Yang Melkamu Mengistnew Teshome +1 位作者 Yu-Zhen Yeh Tamrat Yifter Meles 《Computers, Materials & Continua》 SCIE EI 2024年第6期3519-3547,共29页
In this study,we introduce a novel multi-objective optimization model tailored for modern manufacturing,aiming to mitigate the cost impacts of operational disruptions through optimized corrective maintenance.Central t... In this study,we introduce a novel multi-objective optimization model tailored for modern manufacturing,aiming to mitigate the cost impacts of operational disruptions through optimized corrective maintenance.Central to our approach is the strategic placement of maintenance stations and the efficient allocation of personnel,addressing a crucial gap in the integration of maintenance personnel dispatching and station selection.Our model uniquely combines the spatial distribution of machinery with the expertise of operators to achieve a harmonious balance between maintenance efficiency and cost-effectiveness.The core of our methodology is the NSGA Ⅲ+Dispatch,an advanced adaptation of the Non-Dominated Sorting Genetic Algorithm Ⅲ(NSGA-Ⅲ),meticulously designed for the selection of maintenance stations and effective operator dispatching.This method integrates a comprehensive coding process,crossover operator,and mutation operator to efficiently manage multiple objectives.Rigorous empirical testing,including a detailed analysis from a taiwan region electronic equipment manufacturer,validated the effectiveness of our approach across various scenarios of machine failure frequencies and operator configurations.The findings reveal that the proposed model significantly outperforms current practices by reducing response times by up to 23%in low-frequency and 28.23%in high-frequency machine failure scenarios,leading to notable improvements in efficiency and cost reduction.Additionally,it demonstrates significant improvements in oper-ational efficiency,particularly in selective high-frequency failure contexts,while ensuring substantial manpower cost savings without compromising on operational effectiveness.This research significantly advances maintenance strategies in production environments,providing the manufacturing industry with practical,optimized solutions for diverse machine malfunction situations.Furthermore,the methodologies and principles developed in this study have potential applications in various other sectors,including healthcare,transportation,and energy,where maintenance efficiency and resource optimization are equally critical. 展开更多
关键词 Corrective maintenance multi-objective optimization non-dominated sorting genetic algorithmⅢ operator allocation maintenance station location
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An envelope-based machine learning workflow for locating earthquakes in the southern Sichuan Basin
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作者 Kang Wang Jie Zhang +2 位作者 Ji Zhang Zhangyu Wang Ziyu Li 《Earthquake Research Advances》 CSCD 2024年第2期45-54,共10页
The development of machine learning technology enables more robust real-time earthquake monitoring through automated implementations. However, the application of machine learning to earthquake location problems faces ... The development of machine learning technology enables more robust real-time earthquake monitoring through automated implementations. However, the application of machine learning to earthquake location problems faces challenges in regions with limited available training data. To address the issues of sparse event distribution and inaccurate ground truth in historical seismic datasets, we expand the training dataset by using a large number of synthetic envelopes that closely resemble real data and build an earthquake location model named ENVloc. We propose an envelope-based machine learning workflow for simultaneously determining earthquake location and origin time. The method eliminates the need for phase picking and avoids the accumulation of location errors resulting from inaccurate picking results. In practical application, ENVloc is applied to several data intercepted at different starting points. We take the starting point of the time window corresponding to the highest prediction probability value as the origin time and save the predicted result as the earthquake location. We apply ENVloc to observed data acquired in the southern Sichuan Basin, China, between September 2018 and March 2019. The results show that the average difference with the catalog in latitude, longitude, depth, and origin time is 0.02°,0.02°, 2 km, and 1.25 s, respectively. These suggest that our envelope-based method provides an efficient and robust way to locate earthquakes without phase picking, and can be used in earthquake monitoring in near-real time. 展开更多
关键词 Waveform envelope Earthquake location Local seismicity Synthetic data Sparse stations
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Fault locating for traveling-wave accelerators based on transmission line theory
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作者 Tong-Ning Hu Hai-Meng Wang +4 位作者 Yi-Feng Zeng Hong-Jie Xu Li Chen Guang-Yao Feng Yuan-Ji Pei 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第8期24-34,共11页
Radio-frequency(RF)breakdown analysis and location are critical for successful development of high-gradient traveling-wave(TW)accelerators,especially those expected to generate high-intensity,high-power beams.Compared... Radio-frequency(RF)breakdown analysis and location are critical for successful development of high-gradient traveling-wave(TW)accelerators,especially those expected to generate high-intensity,high-power beams.Compared with commonly used schemes involving dedicated devices or complicated techniques,a convenient approach for breakdown locating based on transmission line(TL)theory offers advantages in the typical constant-gradient TW-accelerating structure.To deliver such an approach,an equivalent TL model has been constructed to equate the TW-accelerating structure based on the fun-damental theory of the TL transient response in the time domain.An equivalence relationship between the TW-accelerating structure and the TL model has been established via analytical derivations associated with grid charts and verified by TL circuit simulations.Furthermore,to validate the proposed fault-locating method in practical applications,an elaborate analysis via such a method has been conducted for the recoverable RF-breakdown phenomena observed at an existing prototype of a TW-accelerating-structure-based beam injector constructed at the Huazhong University of Science and Technology.In addition,further considerations and discussion for extending the applications of the proposed method have been given.This breakdown-locating approach involving the transient response in the framework of TL theory can be a conceivable supple-ment to existing methods,facilitating solution to construction problems at an affordable cost. 展开更多
关键词 Traveling-wave structure RF breakdown Fault locating Transmission line
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Performance Evaluation of Three-Dimensional UWB Real-Time Locating Auto-Positioning System for Fire Rescue
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作者 Hang Yang Xunbo Li Witold Pedrycz 《Intelligent Automation & Soft Computing》 SCIE 2023年第9期3039-3058,共20页
Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on... Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on-site launching and generates real-time location data,enabling fire rescuers to arrive at the intended spot faster and correctly for effective and precise rescue.Auto-positioning with step-by-step instructions is proposed when launching the locating system,while no extra measuring instrument like Total Station(TS)is needed.Real-time location tracking is provided via a 3D space real-time locating system(RTLS)constructed using Ultra-wide Bandwidth technology(UWB),which requires electromagnetic waves to pass through concrete walls.A hybrid weighted least squares with a time difference of arrival(WLS/TDOA)positioning method is proposed to address real path-tracking issues in 3D space and to meet RTLS requirements for quick computing in real-world applications.The 3D WLS/TDOA algorithm is theoretically constructed with the Cramer-Rao lower bound(CRLB).The computing complexity is reduced to the lower bound for embedded hardware to directly compute the time differential of the arriving signals using the time-to-digital converter(TDC).The results of the experiments show that the errors are controlled when the positioning algorithm is applied in various complicated situations to fulfill the requirements of engineering applications.The statistical analysis of the data reveals that the proposed UWB RTLS auto-positioning system can track target tags with an accuracy of 0.20 m. 展开更多
关键词 3D space positioning ULTRA-WIDEBAND real-time locating system time difference of arrival Cramer-Rao lower bound fire rescue
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An online fast multi-track locating algorithm for high-resolution single-event effect test platform 被引量:1
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作者 Yu-Xiao Hu Hai-Bo Yang +3 位作者 Hong-Lin Zhang Jian-Wei Liao Fa-Tai Mai Cheng-Xin Zhao 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第5期86-100,共15页
To improve the efficiency and accuracy of single-event effect(SEE)research at the Heavy Ion Research Facility at Lanzhou,Hi’Beam-SEE must precisely localize the position at which each heavy ion hitting the integrated... To improve the efficiency and accuracy of single-event effect(SEE)research at the Heavy Ion Research Facility at Lanzhou,Hi’Beam-SEE must precisely localize the position at which each heavy ion hitting the integrated circuit(IC)causes SEE.In this study,we propose a fast multi-track location(FML)method based on deep learning to locate the position of each particle track with high speed and accuracy.FML can process a vast amount of data supplied by Hi’Beam-SEE online,revealing sensitive areas in real time.FML is a slot-based object-centric encoder-decoder structure in which each slot can learn the location information of each track in the image.To make the method more accurate for real data,we designed an algorithm to generate a simulated dataset with a distribution similar to that of the real data,which was then used to train the model.Extensive comparison experiments demonstrated that the FML method,which has the best performance on simulated datasets,has high accuracy on real datasets as well.In particular,FML can reach 238 fps and a standard error of 1.6237μm.This study discusses the design and performance of FML. 展开更多
关键词 Beam tracks Multi-track location Rapid location High accuracy Synthetic data Deep neural network Single-event effects Silicon pixel sensors HIRFL
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WORKPIECE LOCATING AND POST PROCESSING SYSTEMS ON 6-DOF CNC MILLING MACHINE
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作者 王瑞 钟诗胜 王知行 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2006年第2期138-143,共6页
A conventional non-computerized numerical control (CNC) machine is updated by mounting a six degree-of-free (DOF) parallel mechanism on it, thus obtaining a new CNC one. The structure of this CNC milling machine i... A conventional non-computerized numerical control (CNC) machine is updated by mounting a six degree-of-free (DOF) parallel mechanism on it, thus obtaining a new CNC one. The structure of this CNC milling machine is introduced, and the workpiece locating system and the post processing system of the cutter location (CL) data file are analyzed. The new machine has advantages of low costs, simple structure, good rigidity, and high precision. It is easy to be transformed and used to process the workpiece with a complex surface. 展开更多
关键词 parallel kinematic machine CNC milling machine workpiece locating system post processing system
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无线网络优化LOCATING过程中ERICSSON3算法的应用
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作者 李志荣 丁远 《轻工科技》 2014年第3期54-55,85,共3页
介绍在LOCATING过程中处理基本排队时所用到的一种算法——ERICSSON 3算法,探讨通过该算法控制切换的方法(只涉及爱立信设备)。
关键词 切换 locating ERICSSON3 优化
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The Present Status and Prospect of Earthquake Focal Depth Locating
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作者 Zheng Yong Xie Zujun 《Earthquake Research in China》 CSCD 2018年第3期330-343,共14页
Locating an earthquakes focal depth is always a key project in seismology. Precise focal depth is of critical importance for evaluating seismic hazards, deciphering dynamic mechanisms of earthquake generating,estima... Locating an earthquakes focal depth is always a key project in seismology. Precise focal depth is of critical importance for evaluating seismic hazards, deciphering dynamic mechanisms of earthquake generating,estimating aftershock evolutions and risk,as well as monitoring nuclear tests. However,how we determine an accurate focal depth is always a challenge in seismological studies. Aiming to solve these problems, we analyzed and summarized the present status and the future development of earthquake focal depth locating. In this paper we first reviewed the present status of focal depth locating in the world,and summarized the frequently-used relocating methods and ideas at present,and introduced two types of focal depth relocating ideas: arrival time relocating and waveform modeling methods. For these ideas,we systematically described the S-P and the Pn-Pg methods that belong to arrival time method,and polarization focal depth locating and amplitude focal depth locating that belongs to waveform modeling,and further analyzed the advantages and limitations of these methods. Since the depth phase methods are highly sensitive to focal depth,and are relatively free from the uncertainties of crustal models,we mainly reviewed the depth phases of s Pm P,s PL,s Pn,and s Sn,and quantitatively evaluated their availabilities and characteristics. Second,we also discussed the effects of crustal velocity models on the reliability of focal depth locating,and reviewed the advancements of seismic tomography techniques over recent years. Finally,based on the present status of the progress on the focal depth locating,and studies of seismic velocity structures,we proposed an idea of combining multiple datasets and relocating methods,jointly utilizing seismologic and geodetic techniques to relocate focal depth,which should be the major research field in investigating focal depth and source parameters in the near future. 展开更多
关键词 FOCAL DEPTH relocating ARRIVAL time locating WAVEFORM modeling DEPTH phase Joint INVERSION
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An intersection method for locating earthquakes in complex velocity models 被引量:1
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作者 赵爱华 丁志峰 《Applied Geophysics》 SCIE CSCD 2007年第4期294-300,共7页
The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity mo... The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity model used for the conventional intersection method is based on homogeneous or laterally homogeneous media, which is too simple. In order to improve the accuracy, we have modified the existing intersection method. In the modified approach, the earthquake loci are not assumed to be circular or hyperbolic and calculation accuracy is improved using a minimum traveltime tree algorithm for tracing rays. The numerical model shows that the modified method can locate earthquakes in complex velocity models. 展开更多
关键词 Earthquake location intersection method ray tracing minimum traveltime tree algorithm
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Topological Optimization Method for Aeronautical Thin-Walled Component Fixture Locating Layout 被引量:2
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作者 Yang Yuan Wang Zhongqi +1 位作者 Yang Bo Kang Yonggang 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2017年第4期405-412,共8页
Fixture locating layout has a direct and influential impact on aeronautical thin-walled component(ATWC)manufacturing quality.The purpose is to develop a topological optimization method for ATWC fixture locating layout... Fixture locating layout has a direct and influential impact on aeronautical thin-walled component(ATWC)manufacturing quality.The purpose is to develop a topological optimization method for ATWC fixture locating layout to minimize the manufacturing deformation.Firstly,a topological optimization model that takes the stiffness of ATWC as the objective function and the volume of the locating structure as the constraint is established.Secondly,ATWC and the locating structure are regarded as an integrated entity,and the variable-density method based topological optimization approach is adopted for the optimization of the locating structure using ABAQUS topology optimization module(ATOM).Thirdly,through a subsequent model reconstruction referring to the obtained topological structure,the optimal fixture locating layout is achieved.Finally,a case study is conducted to verify the proposed method and the comparison results with firefly algorithm(FA)coupled with finite element analysis(FEA)indicate that the number and positions of the locators for ATWC can be optimized simultaneously and successfully by the proposed topological optimization model. 展开更多
关键词 aeronautical thin-walled component fixture locating layout topological optimization variable-density method
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An Integrated Approach to Oscillation Propagation Identification and Source Locating in Process Multi-loop Systems 被引量:1
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作者 曹稼斌 张亮 +1 位作者 郑建荣 夏春明 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2011年第6期999-1008,共10页
An integrated method for identifying the propagation of multi-loop process oscillations and their source location is proposed in this paper. Oscillatory process loop variables are automatically selected based on the c... An integrated method for identifying the propagation of multi-loop process oscillations and their source location is proposed in this paper. Oscillatory process loop variables are automatically selected based on the component-related ratio index and a mixing matrix, both of which are obtained in data preprocessing by spectral independent component analysis. The complex causality among oscillatory process variables is then revealed by Granger causality test and is visualized in the form of causality diagram. The simplification of causal connectivity in the diagram is performed according to the understanding of process knowledge and the final simplest causality diagram, which represents the main oscillation propagation paths, is achieved by the automated cutting-off thresh-old search, with which less significant causality pathways are filtered out. The source of the oscillation disturbance can be identified intuitively through the final causality diagram. Both simulated and real plant data tests are presented to demonstrate the effectiveness and feasibility of the proposed method. 展开更多
关键词 multi-loop system process oscillation spectral independent component analysis Granger causality test causality diagram propagation path simplification source locating
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The clinical research of esophagus radiographic locating method of atrial septal puncture-including the clinical analyses of 486 cases 被引量:1
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作者 Department of Cardiology, Guangzhou Red Cross Hospital, The Fourth Affiliated Hospital of Jinan University, Guangzhou 510220, China (WU Tong-guo, CHEN Si-wei, LW Zi-qing, HUANG Li-ping) School of Biomedical Sciences, Carles Start University, Wagga Wagga, Australia (WANG Le-xing) Department of Cardiology, The Affiliated Hospital of Taishan Medical College, Tai’ an 271000, China(YANG Cheng-jun) 《上海医学》 CAS CSCD 北大核心 2007年第S1期152-152,共1页
Objective To devise the safety and validity of clinical application of esophagus radiographic locating method of transseptal puncture. Methods Four hundreds and eighty-six patients who were undergone transseptal punct... Objective To devise the safety and validity of clinical application of esophagus radiographic locating method of transseptal puncture. Methods Four hundreds and eighty-six patients who were undergone transseptal puncture were randomized into two groups. Esophagus radiographic locating method of tansseptal puncture was applied in the study group(n=243) and modified-Ross locating method was used in the control group(n=243). After successful location, transseptal puncture was made. Then observe the success rate and complication incidences of the 2 groups. Results Left atria impression in the study group was clearly seen. 120 cases in control group could not shown clearly the left atria silhouette. The success rate of locating transseptal puncture in the study group and control group were 100% and 50.6 % respectively(P【0.001). The success rate of transeptal puncture in the study group and control group was 99.6% and 45.7% respectively (P【0.001). There were no puncture complication in study group and pericardial tamponade occurred in 1 case in control group. Conclusion The esophagus radiographic locating method of transeptal puncture is accurate, safe and simple to perform. According to the radiographic result, to perform thetranseptal puncture can achieve high success rate with few complications. 展开更多
关键词 PUNCTURE locating ESOPHAGUS SEPTAL hundreds devise clearly IMPRESSION validity
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An improved QuikSCAT wind retrieval algorithm and eye locating for typhoon 被引量:1
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作者 ZHONG Jian FEI Jianfang +2 位作者 HUANG Sixun DU Huadong ZHANG Liang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第1期41-50,共10页
This paper proposes a rain considered geophysical model function (GMF), to be noted as GMF plus Rain. GMF plus Rain is based on the basic raidative transfer model with attenuation and scattering effects of rain on r... This paper proposes a rain considered geophysical model function (GMF), to be noted as GMF plus Rain. GMF plus Rain is based on the basic raidative transfer model with attenuation and scattering effects of rain on radar signal considered. Combined with the NSCAT2 GMF and the rain correction model, the GMF plus Rain model is used to retrieve the ocean wind vectors from the collocated QuikSCAT and SSM/I rain rate data for typhoon Melor. The resulting wind speed estimates of typhoon Melor show improved agreement with the wind fields derived from the best track analysis of Japan Meteorological Agency (JMA). The results imply that compared with the GMF model, the GMF plus Rain model can improve the precision of wind retrieval under the rain condition. Then, a new general algorithm of locating the eye of typhoon through the normalized radar cross section (NRCS) is proposed. The implementation of this algorithm in the ten QuikSCAT observations of typhoon Melor suggests that this algorithm is effective. 展开更多
关键词 SCATTEROMETER radiative transfer model typhoon wind field locating the eye of typhoon
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RFID Complex Event Processing: Applications in Real-Time Locating System 被引量:2
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作者 Yao-zong Liu Hong Zhang Yong-li Wang 《International Journal of Intelligence Science》 2012年第4期160-165,共6页
Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP,... Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP, which is based on the timed automata (TA) theory. By devising RFID locating application into complex events, we model the timing diagram of RFID data streams based on the TA. We optimize the constraint of the event streams and propose a novel method to derive the constraint between objects, as well as the constraint between object and location. Experiments prove the proposed method reduces the cost of RFID complex event processing, and improves the efficiency of the RTLS. 展开更多
关键词 Complex Event Processing (CEP) REAL-TIME locating System (RTLS) Radio Frequency Identification (RFID) TIMED AUTOMATA (TA) Event-Clock AUTOMATA (ECA)
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Local Spiral Curves Simulating Based on Hough Transformation and Center Auto-Locating of Developing Typhoon 被引量:1
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作者 王萍 郭传澍 罗颖昕 《Transactions of Tianjin University》 EI CAS 2006年第2期142-146,共5页
In the developing phase of typhoon formation, the spiral belt partly emerges in satellite cloud images. This research starts from images and moves on to graphics and then to representation and recognition. Following t... In the developing phase of typhoon formation, the spiral belt partly emerges in satellite cloud images. This research starts from images and moves on to graphics and then to representation and recognition. Following this route, local spiral cloud belt is segmented from the raw images using image segmentation, the spiral information is extracted using mathematic morphology, and local spiral curves are detected using Hough transformation. The problem of center locating of developing typhoon has finally been solved through a search algorithm of spiral curve. For No. 99082008 cloud image, the result produced by the algorithm in this paper is at 122..3 degree west longitude, 117.5 degree north latitude. The real typhoon center location was at 122.4 degree west longitude, 18 degree north latitude. 展开更多
关键词 Hough transformation spiral curve simulating infrared satellite cloud images center locating of developing typhoon
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Study on fault locating technology for satellite power system 被引量:1
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作者 龙兵 姜兴渭 宋政吉 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2005年第5期507-510,共4页
It is currently prevalent to locate faults for a satellite power system based on an expert system, not utilizing all the available information provided by tests. The casual network model for a satellite power system i... It is currently prevalent to locate faults for a satellite power system based on an expert system, not utilizing all the available information provided by tests. The casual network model for a satellite power system is presented. Considerations for failure probability of each component of the power system, the cost of applying each test, the influence of a precedent test result on the next test selection, and an optimal sequential testing algorithm for fault location is presented. This program is applied to locate the failure component of the power system of a satellite. The results show this program is very effective and it is very fast to generate an optimal diagnosis tree. 展开更多
关键词 Satellite power system fault locating casual network model sequential testing
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A Study on the Mobile Partial Discharge Locating System 被引量:1
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作者 Yan Yang Lin Tang +4 位作者 Yue Hu Qian Wang Gaolin Wu Tianchen Zhang Xiuchen Jiang 《Energy and Power Engineering》 2013年第4期589-592,共4页
This paper studies on the Partial Discharge (PD) Locating System based on a mobile array of ultra-high frequency (UHF) antennas and a vehicle-mounted PD locating system is established. The system consists of omni-dire... This paper studies on the Partial Discharge (PD) Locating System based on a mobile array of ultra-high frequency (UHF) antennas and a vehicle-mounted PD locating system is established. The system consists of omni-directional antenna array for receiving UHF PD signals, a pre-processing circuit for signal amplification and filtering and the high- speed acquisition and control unit of PD pulse signals. The developed locating system is able to simultaneously record the PD pulse signals received by the antenna array. By assessing the time difference of arrival (TDOA), the two-dimensional hyperbolic locating model quickly locates the PD source. Based on the software developed by LabVIEW, it is also possible to display, store and further analyze the acquired signals. Through the simulation of PD signals and the locating experiments with the system, it is proved that the PD locating system possesses the features of rapidity and precision in determining the bearing of PD source. 展开更多
关键词 PARTIAL DISCHARGE locating SYSTEM TWO-DIMENSIONAL MODEL MOBILE
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A Two-Station Passive Locating Solution Independent of the Baseline Length 被引量:1
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作者 Tao Yu 《Open Journal of Applied Sciences》 2020年第7期476-485,共10页
The two-station positioning system based on time difference and azimuth measurement has measurement redundancy. Therefore, not only can a positioning solution which is completely independent of the baseline length bet... The two-station positioning system based on time difference and azimuth measurement has measurement redundancy. Therefore, not only can a positioning solution which is completely independent of the baseline length between two stations be derived, but also the baseline length can be solved as an unknown quantity. These findings not only enhance the performance of the two-station positioning system, but also provide a design basis for the construction of a self-organizing dynamic intelligent positioning system. 展开更多
关键词 Positioning without Baseline Two-Station locating Time Difference Direction Finding Path Difference Passive Location Self-Organizing System
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Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats 被引量:1
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作者 Su Liu1, Guangyu Shen1, Guangming Lü2, Xiaosong Gu3 1Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China 2Department of Human Anatomy, Nantong University, Nantong 226001, Jiangsu Province, China 3Jiangsu Key Laboratory of Nerve Regeneration, Nantong 226001, Jiangsu Province, China 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第7期642-644,共3页
BACKGROUND: There are many methods for myelin staining, mordant, or oil-soluble dye or the special reaction of osmic acid with lipoid is used according to different principles. The commonly used methods are classic We... BACKGROUND: There are many methods for myelin staining, mordant, or oil-soluble dye or the special reaction of osmic acid with lipoid is used according to different principles. The commonly used methods are classic Well staining, classic lithium carbonate-haematine staining, fast green staining, silver staining, etc. Luxol Fast Blue can brightly stain myelin sheath, and has certain specificity. The background can be very clean if there is proper differentiation, whereas Luxol Fast Blue is cheap and convenient to operate, thus it is an ideal staining reagent for routine myelin sheath. OBJECTIVE: To show the corticospinal tract of normal adult rats with Luxol Fast Blue staining method. DESIGN: A repetitive measurement design. SETTINGS: Institute of Nuerobiology, Nantong University; Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University. MATERIALS: Six healthy adult male SD rats of clean degree, weighing averagely 300 g, were provided by the experimental animal center of Nantong University. 1 g/L Luxol Fast Blue solution was provided by Sigma Company; Leica CM1900 cryostat microtome by Leica Company; Leica DMR microscope by Leica Company. METHODS: The experiment was carried out in the Staff Room of Human Anatomy, Nantong University in May 2005. The rats were given intraperitoneal injection of combined anesthetic (2 mL/kg), then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed, then changed to phosphate buffer (pH 7.4) containing 300 g/L saccharu at 4 ℃, and stayed overnight, tissue blocks at pyramid, decussation of pyramid and cervical, thoracic, lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections (30 μm) after sedimentation, the sections were dried at room temperature. The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method, and observed under Leica DMR microscope. MAIN OUTCOME MEASURES: Positive fibers in Luxol Fast Blue staining. RESULTS: After the Luxol Fast Blue staining, the labeled myelinated nerve fibers were bright blue. They located in the pyramid, decussation of pyramid and the ventral part of posterior funiculus in cervical, thoracic, lumbar and sacral segments of spinal cord. CONCLUSION: Luxol Fast Blue staining method may manifest the distribution of corticospinal tract with clear distinct in adult rats. 展开更多
关键词 Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats
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浅谈如何启用上行LOCATING算法减少掉话 被引量:1
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作者 石艳 李远忠 《移动通信》 2003年第04B期47-50,共4页
在爱立隹GSM(全球通)网络中,切换定位算法一般是采用下行信号强度K算法,关闭上行算法。本文通过理论和实验说明如何启用上行LOCATING(定位)算法减少掉话,改善网络质量。
关键词 GSM 切换定位 网络质量 下行信号强度 掉话 上行locating算法
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