Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substit...Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substitution line in which a pair of intact chromosomes of TM_1 ( G. hirsutum ) were replaced by a pair of homozygous chromosomes of 3_79 ( G. barbadense ) is an excellent material for genetic research and molecular tagging. In this study, substitution line 16 (Sub 16) was used to evaluate the performance of the 16th chromosome in G. barbadense in TM_1 background. The genetic analysis using the major gene plus polygene mixed inheritance model in F 2∶3 family revealed that there might exist 2 QTLs respectively for boll size, lint percentage, lint index, fiber length and the first fruit branch node, 1 QTL for fiber elongation and flowering date, and no QTL for seed index, fiber strength and Micronaire in chromosome 16. However, 9 QTLs (LOD (logarithm of odds)≥3.0) controlling 6 quantitative traits were significantly identified in linkage group of chromosome 16 constructed in (TM_1×3_79) F 2by interval mapping. Among them, 1 QTL for boll size, fiber length, flowering date and fiber elongation could explain 15.2%, 19.7%, 12.1%, and 11.7% phenotypic variance respectively, 2 QTLs for lint index could explain 11.6% and 41.9%, and 3 QTLs for lint percentage could explain 8.7%, 9.6% and 29.2% phenotypic variance respectively. One unlinked SSR marker was associated with one QTL respectively for boll size and flowering date and they could explain 1.60% and 4.63% phenotypic variance. The traits associated significantly with chromosome 16 from Sub 16 were boll weight, lint percentage, lint index, fiber length, fiber elongation and flowering days.展开更多
谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,...谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,为类黄酮合成关键基因的精细定位、克隆及功能研究奠定基础,同时,也为揭示谷子类黄酮合成及代谢机制和培育富含类黄酮谷子品种提供技术支撑。本研究以红粒色高类黄酮品种金苗红酒谷和黄粒色低类黄酮品种豫谷28为亲本构建的包含150个家系的重组自交系(RIL)群体为试验材料,在谷子成熟期对籽粒粒色和类黄酮含量相关性状进行分析。同时,采用复合区间作图法(composite interval mapping,CIM)对粒色和类黄酮含量进行QTL定位与分析,并对QTL置信区间内的候选基因进行预测。相关性分析表明,类黄酮含量与粒色呈显著正相关。共定位到4个与类黄酮含量相关和11个与粒色相关的QTL,分别位于1号、2号、5号、6号、7号、8号和9号染色体上,单个QTL的表型贡献率为2.01%~29.25%,6个为主效QTL,其中,qSC1-2和qFLA1-1、qSC7-1和qFLA7-1、qSC9-3和qFLA9-1为2个性状下共同定位到的QTL。通过基因预测与功能注释,筛选出QTL置信区间内5个与类黄酮物质合成及代谢相关的候选基因,表明类黄酮物质的合成、代谢及利用相关基因极有可能控制了这些基因的表达。15个QTL分别聚集于7条染色体上,基于基因功能注释,共筛选了5个与谷子类黄酮合成及代谢相关的候选基因,表明不同QTL位点参与到了共同遗传机制,并可通过分子标记辅助选择进行类黄酮合成及代谢等有利基因的聚合育种。展开更多
Improved seed composition in soybean [Glycine max (L.) Merr.] for protein and oil quality is one of the major goals of soybean breeders. A group of genes that act as quantitative traits with their effects can alter pr...Improved seed composition in soybean [Glycine max (L.) Merr.] for protein and oil quality is one of the major goals of soybean breeders. A group of genes that act as quantitative traits with their effects can alter protein, oil, palmitic, stearic, oleic, linoleic, and linolenic acids percentage in soybean seeds. The objective of this study was to identify Quantitative Trait Loci (QTL) controlling protein, oil, and fatty acids content in a set of F5:8 RILs derived from a cross between lines, ‘MD 96-5722’ and ‘Spencer’ using 5376 Single Nucleotide Polymorphism (SNP) markers from the Illumina Infinium SoySNP6K BeadChip array. QTL analysis used WinQTL Cart 2.5 software for composite interval mapping (CIM). Identified, were;one protein content QTL on linkage group (LG-) B2 or chromosome (Chr_) 14;11 QTL associated with oil content on six linkage groups LG-N (Chr_3), LG-A1 (Chr_5), LG-K (Chr_9), LG-F (Chr_13), LG-B2 (Chr_14), and LG-J (Chr_16);and sixteen QTL for five major fatty acids (palmitic, stearic, oleic, linoleic, and linolenic acids) on LG-N (Chr_3), LG-F (Chr_13), LG-B2 (Chr_14), LG-E (Chr_15), LG-J (Chr_16), and LG-G (Chr_18). The SNP markers closely linked to the QTL reported here will be useful for development of cultivars with altered oil and fatty acid compositions in soybean breeding programs.展开更多
Rose is one of the most important ornamental and economic plants in the world.Modern rose cultivars are primarily tetraploid,and during meiosis,they may exhibit double reduction or preferential chromosome pairing.Ther...Rose is one of the most important ornamental and economic plants in the world.Modern rose cultivars are primarily tetraploid,and during meiosis,they may exhibit double reduction or preferential chromosome pairing.Therefore,the construction of a high density genetic map of tetraploid rose is both challenging and instructive.In this study,a tetraploid rose population was used to conduct a genetic analysis using genome sequencing.A total of 17382 single nucleotide polymorphism(SNP)markers were selected from 2308042 detected SNPs.Combined with 440 previously developed simple sequence repeats(SSR)and amplified fragment length polymorphism(AFLP)markers,a marker dosage of 6885 high quality markers was successfully assigned by GATK software in the tetraploid model.These markers were used in the construction of a high density genetic map,containing the expected seven linkage groups with 6842 markers,a total map length of 1158.9 c M,and an average inter-marker distance of 0.18 c M.Quantitative trait locus(QTL)analysis was subsequently performed to characterize the genetic architecture of petal number and flower diameter.One major QTL(qpnum-3-1)was detected for petal number in three consecutive years,which explained 20.18–22.11%of the variation in petal number.Four QTLs were detected for flower diameter;the main locus,qfdia-2-2,was identified in two consecutive years.Our results will benefit the molecular marker-assisted breeding of modern rose cultivars.In addition,this study provides a guide for the genetic and QTL analysis of autotetraploid plants using sequencing-based genotyping methods.展开更多
文摘Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substitution line in which a pair of intact chromosomes of TM_1 ( G. hirsutum ) were replaced by a pair of homozygous chromosomes of 3_79 ( G. barbadense ) is an excellent material for genetic research and molecular tagging. In this study, substitution line 16 (Sub 16) was used to evaluate the performance of the 16th chromosome in G. barbadense in TM_1 background. The genetic analysis using the major gene plus polygene mixed inheritance model in F 2∶3 family revealed that there might exist 2 QTLs respectively for boll size, lint percentage, lint index, fiber length and the first fruit branch node, 1 QTL for fiber elongation and flowering date, and no QTL for seed index, fiber strength and Micronaire in chromosome 16. However, 9 QTLs (LOD (logarithm of odds)≥3.0) controlling 6 quantitative traits were significantly identified in linkage group of chromosome 16 constructed in (TM_1×3_79) F 2by interval mapping. Among them, 1 QTL for boll size, fiber length, flowering date and fiber elongation could explain 15.2%, 19.7%, 12.1%, and 11.7% phenotypic variance respectively, 2 QTLs for lint index could explain 11.6% and 41.9%, and 3 QTLs for lint percentage could explain 8.7%, 9.6% and 29.2% phenotypic variance respectively. One unlinked SSR marker was associated with one QTL respectively for boll size and flowering date and they could explain 1.60% and 4.63% phenotypic variance. The traits associated significantly with chromosome 16 from Sub 16 were boll weight, lint percentage, lint index, fiber length, fiber elongation and flowering days.
文摘谷子(Setaria italica L.)是我国北方地区重要的粮食作物,籽粒营养丰富,且富含多种类黄酮物质,对生长发育和品质形成发挥着重要作用。目前谷子籽粒类黄酮合成及粒色形成相关调控机制研究较少。分析谷子类黄酮含量及粒色性状相关的QTL,为类黄酮合成关键基因的精细定位、克隆及功能研究奠定基础,同时,也为揭示谷子类黄酮合成及代谢机制和培育富含类黄酮谷子品种提供技术支撑。本研究以红粒色高类黄酮品种金苗红酒谷和黄粒色低类黄酮品种豫谷28为亲本构建的包含150个家系的重组自交系(RIL)群体为试验材料,在谷子成熟期对籽粒粒色和类黄酮含量相关性状进行分析。同时,采用复合区间作图法(composite interval mapping,CIM)对粒色和类黄酮含量进行QTL定位与分析,并对QTL置信区间内的候选基因进行预测。相关性分析表明,类黄酮含量与粒色呈显著正相关。共定位到4个与类黄酮含量相关和11个与粒色相关的QTL,分别位于1号、2号、5号、6号、7号、8号和9号染色体上,单个QTL的表型贡献率为2.01%~29.25%,6个为主效QTL,其中,qSC1-2和qFLA1-1、qSC7-1和qFLA7-1、qSC9-3和qFLA9-1为2个性状下共同定位到的QTL。通过基因预测与功能注释,筛选出QTL置信区间内5个与类黄酮物质合成及代谢相关的候选基因,表明类黄酮物质的合成、代谢及利用相关基因极有可能控制了这些基因的表达。15个QTL分别聚集于7条染色体上,基于基因功能注释,共筛选了5个与谷子类黄酮合成及代谢相关的候选基因,表明不同QTL位点参与到了共同遗传机制,并可通过分子标记辅助选择进行类黄酮合成及代谢等有利基因的聚合育种。
文摘Improved seed composition in soybean [Glycine max (L.) Merr.] for protein and oil quality is one of the major goals of soybean breeders. A group of genes that act as quantitative traits with their effects can alter protein, oil, palmitic, stearic, oleic, linoleic, and linolenic acids percentage in soybean seeds. The objective of this study was to identify Quantitative Trait Loci (QTL) controlling protein, oil, and fatty acids content in a set of F5:8 RILs derived from a cross between lines, ‘MD 96-5722’ and ‘Spencer’ using 5376 Single Nucleotide Polymorphism (SNP) markers from the Illumina Infinium SoySNP6K BeadChip array. QTL analysis used WinQTL Cart 2.5 software for composite interval mapping (CIM). Identified, were;one protein content QTL on linkage group (LG-) B2 or chromosome (Chr_) 14;11 QTL associated with oil content on six linkage groups LG-N (Chr_3), LG-A1 (Chr_5), LG-K (Chr_9), LG-F (Chr_13), LG-B2 (Chr_14), and LG-J (Chr_16);and sixteen QTL for five major fatty acids (palmitic, stearic, oleic, linoleic, and linolenic acids) on LG-N (Chr_3), LG-F (Chr_13), LG-B2 (Chr_14), LG-E (Chr_15), LG-J (Chr_16), and LG-G (Chr_18). The SNP markers closely linked to the QTL reported here will be useful for development of cultivars with altered oil and fatty acid compositions in soybean breeding programs.
基金the National Natural Science Foundation of China(31600565)the Fundamental Research Funds for the Central Public Welfare Research Institutes,China(ZZ13-YQ-053)+1 种基金the Special Fund for Beijing Common Construction Project,ChinaDr.Peter M.Bourke from Plant Breeding,Wageningen University&Research,The Netherlands,was partly funded through the TKI polyploids project(BO-26.03-009-004 and BO-50-002-022)。
文摘Rose is one of the most important ornamental and economic plants in the world.Modern rose cultivars are primarily tetraploid,and during meiosis,they may exhibit double reduction or preferential chromosome pairing.Therefore,the construction of a high density genetic map of tetraploid rose is both challenging and instructive.In this study,a tetraploid rose population was used to conduct a genetic analysis using genome sequencing.A total of 17382 single nucleotide polymorphism(SNP)markers were selected from 2308042 detected SNPs.Combined with 440 previously developed simple sequence repeats(SSR)and amplified fragment length polymorphism(AFLP)markers,a marker dosage of 6885 high quality markers was successfully assigned by GATK software in the tetraploid model.These markers were used in the construction of a high density genetic map,containing the expected seven linkage groups with 6842 markers,a total map length of 1158.9 c M,and an average inter-marker distance of 0.18 c M.Quantitative trait locus(QTL)analysis was subsequently performed to characterize the genetic architecture of petal number and flower diameter.One major QTL(qpnum-3-1)was detected for petal number in three consecutive years,which explained 20.18–22.11%of the variation in petal number.Four QTLs were detected for flower diameter;the main locus,qfdia-2-2,was identified in two consecutive years.Our results will benefit the molecular marker-assisted breeding of modern rose cultivars.In addition,this study provides a guide for the genetic and QTL analysis of autotetraploid plants using sequencing-based genotyping methods.
