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白背飞虱、灰飞虱mariner转座子的初步研究 被引量:3
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作者 黄立华 杜建光 +1 位作者 程遐年 洪晓月 《昆虫学报》 CAS CSCD 北大核心 2001年第4期402-407,共6页
根据毛里塔尼亚果蝇Drosophilamauritania的mariner转座子序列设计简并引物 ,以白背飞虱Sogatellafurcifera和灰飞虱Laodelphaxstriatellus基因组DNA为模板 ,通过PCR反应扩增出了约 5 0 0bp的条带 ,经回收、克隆、测序 ,获得其核苷酸序... 根据毛里塔尼亚果蝇Drosophilamauritania的mariner转座子序列设计简并引物 ,以白背飞虱Sogatellafurcifera和灰飞虱Laodelphaxstriatellus基因组DNA为模板 ,通过PCR反应扩增出了约 5 0 0bp的条带 ,经回收、克隆、测序 ,获得其核苷酸序列 ,与毛里塔尼亚果蝇mariner序列相比 ,核苷酸同源性均为 71 3%,氨基酸同源性分别为 6 9 5 %和 72 %,证明白背飞虱和灰飞虱体内确实存在mariner转座子。 展开更多
关键词 mariner转座子 白背飞虱 灰飞虱 昆虫 基因载体
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Tc1/Mariner转座子超家族的研究进展 被引量:2
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作者 沈丹 陈才 +3 位作者 王赛赛 陈伟 高波 宋成义 《遗传》 CAS CSCD 北大核心 2017年第1期1-13,共13页
随着高通量测序技术的迅猛发展,越来越多的生物基因组注释结果表明:转座子几乎存在于所有生物的基因组中,是大多数生物基因组的重要组分。其中,Tc1/Mariner转座子是自然界中分布最广泛的一类DNA转座子超家族,在自然界已经发现14个有活性... 随着高通量测序技术的迅猛发展,越来越多的生物基因组注释结果表明:转座子几乎存在于所有生物的基因组中,是大多数生物基因组的重要组分。其中,Tc1/Mariner转座子是自然界中分布最广泛的一类DNA转座子超家族,在自然界已经发现14个有活性的Tc1/Mariner转座子(如Minos,Mos1等),另外通过分子重构也获得高活性的人工转座子,如睡美人转座子(Sleeping Beauty,SB)。SB和Mos1等转座子作为基因转移载体已被广泛应用于转基因、基因捕获和基因治疗等领域的研究中,并取得了很好的应用效果。本文将重点综述Tc1/Mariner转座子的结构、分类、分布、转座机制、活性转座子的挖掘,及其在转基因、基因捕获和基因治疗等研究领域的应用。 展开更多
关键词 Tc1/mariner转座子 转基因 基因治疗 基因捕获
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昆虫中的mariner类转座子 被引量:1
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作者 肖巧学 曹阳 +1 位作者 黄自然 刘良式 《遗传》 CAS CSCD 北大核心 2000年第2期114-118,共5页
关键词 mariner类转座子 昆虫 分布 转座机制 调节机制
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Mariner转座子与小菜蛾抗性关系的研究
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作者 于光 程罗根 +3 位作者 张晓飞 陈之浩 李忠英 李凤良 《植物保护》 CAS CSCD 北大核心 2004年第1期21-25,共5页
以抗溴氰菊酯、杀虫双、杀螟丹小菜蛾种群及其敏感品系为研究对象,借助聚合酶链式反应(PCR)、载体筛选、琼脂糖凝胶电泳等实验技术,检测了小菜蛾4个品系的Mariner转座子的存在及其与抗性的关系。结果表明,在抗溴氰菊酯、杀虫双、杀螟丹... 以抗溴氰菊酯、杀虫双、杀螟丹小菜蛾种群及其敏感品系为研究对象,借助聚合酶链式反应(PCR)、载体筛选、琼脂糖凝胶电泳等实验技术,检测了小菜蛾4个品系的Mariner转座子的存在及其与抗性的关系。结果表明,在抗溴氰菊酯、杀虫双、杀螟丹以及敏感品系的小菜蛾中都存在两种大约500 bp的Mariner转座子片断,初次证明在小菜蛾4个品系中均含有两种Mariner转座子,并发现一种新的转座子基因片断,但是没有发现Mariner转座子与抗性之间存在直接的联系。研究结果将为利用Mariner转座子标签法在小菜蛾中分离定位基因、转化外源基因、转基因昆虫防治害虫等研究提供理论基础。 展开更多
关键词 mariner转座子 小菜蛾 抗性 转基因载体 相关性 PCR 农业害虫
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白端按蚊(ANOPHELESALBIMANUS)中MARINER因子的鉴定 被引量:1
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作者 刘克义 Liza.,PM 《中国寄生虫病防治杂志》 CSCD 1995年第2期96-99,共4页
由于转座因子“Marinerelement”在昆虫中可垂直亦可横向传播,携带此因子后昆虫眼睛变色,故而是一种极有发展前途的将杀虫基因转入按蚊的载体。在非洲的冈比亚按蚊中已发现有此因子的存在。本研究通过计算机队列分析,... 由于转座因子“Marinerelement”在昆虫中可垂直亦可横向传播,携带此因子后昆虫眼睛变色,故而是一种极有发展前途的将杀虫基因转入按蚊的载体。在非洲的冈比亚按蚊中已发现有此因子的存在。本研究通过计算机队列分析,从一些昆虫中查出Mariner因子的保守区,并以此为基础设计出PCR简并引物,通过扩增技术,从白端按蚊的基因组中发现了预期的基因片段,表明白端按蚊也存在Mariner因子。 展开更多
关键词 白端按蚊 基因 mariner因子 鉴定
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mariner转座子及其在昆虫种系转化中的应用
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作者 范晓东 李春峰 +1 位作者 刘文明 周泽扬 《蚕学通讯》 2005年第3期12-16,共5页
转座子是不需要同源重组便能在基因组内和基因组间移动的遗传学顺式元件。mari-ner是最简单的真核转座子,在昆虫中广泛分布。mariner转座子的转座作用只与转座酶有关,是一种很有发展前景的转基因载体,可以实现异源昆虫间的基因转移,产... 转座子是不需要同源重组便能在基因组内和基因组间移动的遗传学顺式元件。mari-ner是最简单的真核转座子,在昆虫中广泛分布。mariner转座子的转座作用只与转座酶有关,是一种很有发展前景的转基因载体,可以实现异源昆虫间的基因转移,产生稳定遗传的转基因品系。 展开更多
关键词 mariner转座子 转座机制 转基因 mariner转座子 昆虫 应用 转化 种系 转基因载体 转基因品系 顺式元件 同源重组
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家蚕类mariner转座子相关序列分析
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作者 张梅 曹广力 +1 位作者 薛仁宇 贡成良 《江苏蚕业》 2008年第2期6-12,共7页
通过PCR克隆,从家蚕基因组中获得大小为975bp的DNA片段(登录号:EU352872),Blast搜寻结果显示,该片段DNA序列的52-449nt区域与野蚕mariner-like元件DNA(登录号:AB237562)的同源性达90%,推测为家蚕类mariner转座子相关序列,在乙酰胆碱酯... 通过PCR克隆,从家蚕基因组中获得大小为975bp的DNA片段(登录号:EU352872),Blast搜寻结果显示,该片段DNA序列的52-449nt区域与野蚕mariner-like元件DNA(登录号:AB237562)的同源性达90%,推测为家蚕类mariner转座子相关序列,在乙酰胆碱酯酶基因的第5内含子、微卫星S1104-R序列、BmBRC-NZ3 mRNA终止码的下游序列、ErB·1基因的内含子均检测到家蚕类mariner元件相关序列的同源区,暗示家蚕类mariner元件在家蚕基因组中发生了多次转座,并在家蚕基因组的不同区域随着进化发生了歧化。 展开更多
关键词 家蚕 mariner转座子 转座
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转座元件mariner 被引量:6
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作者 张卉 王小珂 马世俊 《遗传》 CAS CSCD 北大核心 2004年第5期756-762,共7页
自mariner转座元件在Drosophilamauritiana中首次发现至今已经在包括人类在内的多种生物体中证实了mariner及类mariner元件(MLEs)的存在。MLEs属于mariner/Tc1超家族 II型转座元件中分布最广、种类最多的超家族之一。MLEs的转座酶都具有... 自mariner转座元件在Drosophilamauritiana中首次发现至今已经在包括人类在内的多种生物体中证实了mariner及类mariner元件(MLEs)的存在。MLEs属于mariner/Tc1超家族 II型转座元件中分布最广、种类最多的超家族之一。MLEs的转座酶都具有"D,D(34)D"的结构,并能催化MLEs通过"剪切和粘贴"机制进行转座。它们的宿主广泛和多样,能够进行种系传递,这都表明MLEs的转座不需要宿主特异元件的参与。MLEs对多种生物尤其对脊椎动物的成功转化更支持了它们的不依赖宿主的转座机制,而且让人们看到了它们作为转基因载体的巨大潜能。 展开更多
关键词 mariner MLEs 转座元件 种系转化 转化载体
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Tc1/Mariner转座子超家族在褶皱臂尾轮虫中的进化和表达分析
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作者 范正杰 王玉珏 张全启 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2022年第4期62-74,共13页
为了探究Tc1/Mariner转座子超家族对褶皱臂尾轮虫(Brachionus plicatilis)基因组结构和基因表达调控的影响,本研究基于褶皱臂尾轮虫的基因组和转录组数据,对褶皱臂尾轮虫的重复序列进行了注释,并对褶皱臂尾轮虫的转座子的表达和其临近... 为了探究Tc1/Mariner转座子超家族对褶皱臂尾轮虫(Brachionus plicatilis)基因组结构和基因表达调控的影响,本研究基于褶皱臂尾轮虫的基因组和转录组数据,对褶皱臂尾轮虫的重复序列进行了注释,并对褶皱臂尾轮虫的转座子的表达和其临近基因的表达进行了统计和富集,在基因上下游及内部区域富集到了10个转座子家族(其中3个家族属于Tc1/Mariner超家族)。在针对褶皱臂尾轮虫的Tc1/Mariner超家族分析中,共发现其7个亚家族:Tc1(DD34E)、Tc2(DD35D)、Mariner(DD34D)、Pogo(DDxD)、Sagan(DD30D)、Tigger(DD32/36D)和Fot1(DD30D),并主要分布在基因间区,对转座酶催化区域的结构和系统发育分析显示拥有完整转座酶的3个亚家族(Tc1、Tc2和Pogo)保守性强且聚类情况良好。基于转录组数据发现各个家族的表达模式较为相似,在雄性阶段表达较高。Tc1/Mariner超家族临近基因的功能大量涉及到环境信息处理和生物发育调节,反映了褶皱臂尾轮虫基因组对环境的适应。本研究通过对褶皱臂尾轮虫Tc1/Mariner转座子超家族在基因组中的进化和表达进行系统分析,为从功能角度认知Tc1/Mariner转座子并理解Tc1/Mariner对基因组进化的作用提供了新的线索。 展开更多
关键词 褶皱臂尾轮虫 Tc1/mariner 转座子 系统发育 表达分析
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Mariner转座子简介
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作者 刘娣 关伟军 +3 位作者 祖延涛 杨秀芹 刘和平 张向喆 《国外畜牧科技》 2000年第2期23-24,共2页
关键词 转座子 遗传因子 基因 mariner转座子
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The Images in The Rime of the Ancient Mariner
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作者 XING Fang-fang HUA Yan 《Journal of Literature and Art Studies》 2017年第6期675-678,共4页
The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydif... The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydifferent images which also have different meanings and connotations in the context. What's more, the poemexpresses the theme of the metabolic relationships between men and nature: harmony, conflict or compromise. 展开更多
关键词 The RIME of the ANCIENT mariner S. T. Coleridge IMAGES
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Bartleby and The Anclent Mariner:Parallel Texts
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作者 William Slaughter 《解放军外国语学院学报》 CSSCI 1989年第1期1-3,29,共4页
The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense o... The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense of Bartleby. 展开更多
关键词 Bartleby and The Anclent mariner
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The Wind in"The Rime of the Ancient Mariner
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作者 Sun Peipu 《山东理工大学学报(社会科学版)》 1995年第2期75-77,共3页
Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "T... Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "The Rime of the Ancient Mariner"belongs to the second group. Some critics blame Coleridge for his lack of morals in this poem, but in fact,this poem contains lots of morals, as Coleridge himself said.Coleridge succeeds in exploring the theme of sin and punishment by his rich imagination and by the depicting of supernatural things. In "The Rime of the Ancient Mariner", Coleridge intentionally built a symbolic world of the supernatural wind, the bird, the sun, the moon ,the ship and the sea. All these symbols serve to develop the theme of Fall and Redemption, and play so important a part in the poem that we even can take it as a symbolic poem. In the following pages, I am going to devote to the functions of the supernatural wind in particular. Coleridge bestows the wind with a great deal of 展开更多
关键词 WIND The Wind in"The Rime of the Ancient mariner
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A bout the Rime of the Ancient Mariner
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作者 Xing Dong 《朝阳师专学报》 1987年第3期44-46,共3页
One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story t... One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story the spell of th e supernatural, and th e sustained a rchaic style,all con tribu te to make it an unforgettable Poem,PoPular alive with young and old.This article is going to deal with th ree questions:(1)the them of the Poem ( 2 ) the function of the color images and their symbolic representation ?(3) the conceP t of Life一in—Death ? 展开更多
关键词 ANCIENT mariner nineteenth CENTURY BALLAD WRITING
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Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)
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作者 Janine T.Bossé Yanwen Li +11 位作者 Leon G.Leanse Liqing Zhou Roy R.Chaudhuri Sarah E.Peters Jinhong Wang Gareth A.Maglennon Matthew T.G.Holden Duncan J.Maskell Alexander W.Tucker Brendan W.Wren Andrew N.Rycroft Paul R.Langford on behalf of the BRaDPT consortium 《Animal Diseases》 2021年第4期249-261,共13页
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as T... Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines. 展开更多
关键词 mariner TRANSPOSON TraDIS PASTEURELLACEAE Actinobacillus pleuropneumoniae Pasteurella multocida
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The Cradle of China's First Class Seafarers:Qingdao Ocean Shipping Mariners College——by Lin Jinhe,President of Qingdao Ocean Shipping Mariners College
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《中国远洋航务》 1998年第11期18-19,共2页
Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of... Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of Marine Engineering, Department of Shipping Management and Department of Foreign Languages, offering speciahies of Marine Navigation, Marine Engine Control, Marine Electrical Equipment Control, Marine Radio Communication, International Shipping Management and International Finance and Accounting. We have 3 types of education: the education of formal college schooling, the training for implementation of the 95 revised STCW Convention and the continuation education. Meanwhile we undertake different training of COSCO’s contracted seafarers overseas and other managerial per- 展开更多
关键词 by Lin Jinhe President of Qingdao Ocean Shipping mariners College The Cradle of China’s First Class Seafarers
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一个新的mariner类转座子的鉴定 被引量:1
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作者 李秀兰 姜曰水 曲志才 《应用与环境生物学报》 CAS CSCD 北大核心 2010年第4期541-544,共4页
根据mariner类转座子转座酶的保守区设计简并引物,以曲阜灰飞虱的基因组DNA为模板,通过PCR扩增、回收、克隆和测序,得到长为493bp的片段,命名为qfclone493,提交GenBank数据库,获得注册号为EU542024.将qfclone493与19种有代表性的marine... 根据mariner类转座子转座酶的保守区设计简并引物,以曲阜灰飞虱的基因组DNA为模板,通过PCR扩增、回收、克隆和测序,得到长为493bp的片段,命名为qfclone493,提交GenBank数据库,获得注册号为EU542024.将qfclone493与19种有代表性的mariner类转座子核苷酸序列进行系统学分析,结果表明qfclone493属于mauritiana亚族,与此亚族已知mariner类转座子的核苷酸序列同源性最高为74%,认为qfclone493是mauritiana亚族的一个新的mariner类转座子,命名为lsmar1.序列分析表明,lsmar1的最大ORF仅编码141个氨基酸,不能表达完整的转座酶;与隶属于同一亚族、且具有完全自主活性的mos1相比,lsmar1所编码的氨基酸序列在核定位信号(Nuclear location signal,NLS)和D,D(34)D关键部位发生突变,因此推测lsmar1可能不具有转座活性. 展开更多
关键词 灰飞虱 mariner类转座子 序列分析 系统学分析
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面向FEM快速建模的船体模型数据处理算法
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作者 李瑞 王明发 +2 位作者 孟昭闯 刘国磊 邱永添 《中国舰船研究》 CSCD 北大核心 2024年第5期49-56,共8页
[目的]为提高船体有限元几何建模效率,解决船舶设计软件导出的船体模型无法直接应用于有限元几何建模的问题,提出一种基于XML文件数据的几何模型处理方法。[方法]以AVEVA Marine(AM)软件导出的船体XML文件为研究对象,通过对文件的数据... [目的]为提高船体有限元几何建模效率,解决船舶设计软件导出的船体模型无法直接应用于有限元几何建模的问题,提出一种基于XML文件数据的几何模型处理方法。[方法]以AVEVA Marine(AM)软件导出的船体XML文件为研究对象,通过对文件的数据结构解析,明确几何模型的数据表达规则,依据船体有限元几何建模规范,通过修改模型数据实现对船体模型的几何修正,包括对角隅、开孔等构件的删除、简化处理,对球扁钢和肘板的转化处理,对型材和板缝的移动处理。[结果]实际运用结果表明,处理后的船体几何模型可直接导入有限元软件HyperMesh中,符合有限元实体几何建模规范,保留了原模型材料、板架厚度等相关属性。[结论]与人工建模相比,测试分段实体几何建模效率显著提高,所提方法有助于船体设计模型复用、提高有限元几何建模效率。 展开更多
关键词 快速有限元分析 船体模型 模型数据 数据转换 XML格式 AVEVA Marine软件
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Correcting Climate Model Sea Surface Temperature Simulations with Generative Adversarial Networks:Climatology,Interannual Variability,and Extremes 被引量:2
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作者 Ya WANG Gang HUANG +6 位作者 Baoxiang PAN Pengfei LIN Niklas BOERS Weichen TAO Yutong CHEN BO LIU Haijie LI 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第7期1299-1312,共14页
Climate models are vital for understanding and projecting global climate change and its associated impacts.However,these models suffer from biases that limit their accuracy in historical simulations and the trustworth... Climate models are vital for understanding and projecting global climate change and its associated impacts.However,these models suffer from biases that limit their accuracy in historical simulations and the trustworthiness of future projections.Addressing these challenges requires addressing internal variability,hindering the direct alignment between model simulations and observations,and thwarting conventional supervised learning methods.Here,we employ an unsupervised Cycle-consistent Generative Adversarial Network(CycleGAN),to correct daily Sea Surface Temperature(SST)simulations from the Community Earth System Model 2(CESM2).Our results reveal that the CycleGAN not only corrects climatological biases but also improves the simulation of major dynamic modes including the El Niño-Southern Oscillation(ENSO)and the Indian Ocean Dipole mode,as well as SST extremes.Notably,it substantially corrects climatological SST biases,decreasing the globally averaged Root-Mean-Square Error(RMSE)by 58%.Intriguingly,the CycleGAN effectively addresses the well-known excessive westward bias in ENSO SST anomalies,a common issue in climate models that traditional methods,like quantile mapping,struggle to rectify.Additionally,it substantially improves the simulation of SST extremes,raising the pattern correlation coefficient(PCC)from 0.56 to 0.88 and lowering the RMSE from 0.5 to 0.32.This enhancement is attributed to better representations of interannual,intraseasonal,and synoptic scales variabilities.Our study offers a novel approach to correct global SST simulations and underscores its effectiveness across different time scales and primary dynamical modes. 展开更多
关键词 generative adversarial networks model bias deep learning El Niño-Southern Oscillation marine heatwaves
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Sparse Modal Decomposition Method Addressing Underdetermined Vortex-Induced Vibration Reconstruction Problem for Marine Risers 被引量:1
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作者 DU Zun-feng ZHU Hai-ming YU Jian-xing 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期285-296,共12页
When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fa... When investigating the vortex-induced vibration(VIV)of marine risers,extrapolating the dynamic response on the entire length based on limited sensor measurements is a crucial step in both laboratory experiments and fatigue monitoring of real risers.The problem is conventionally solved using the modal decomposition method,based on the principle that the response can be approximated by a weighted sum of limited vibration modes.However,the method is not valid when the problem is underdetermined,i.e.,the number of unknown mode weights is more than the number of known measurements.This study proposed a sparse modal decomposition method based on the compressed sensing theory and the Compressive Sampling Matching Pursuit(Co Sa MP)algorithm,exploiting the sparsity of VIV in the modal space.In the validation study based on high-order VIV experiment data,the proposed method successfully reconstructed the response using only seven acceleration measurements when the conventional methods failed.A primary advantage of the proposed method is that it offers a completely data-driven approach for the underdetermined VIV reconstruction problem,which is more favorable than existing model-dependent solutions for many practical applications such as riser structural health monitoring. 展开更多
关键词 motion reconstruction vortex-induced vibration(VIV) marine riser modal decomposition method compressed sensing
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