本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母...本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母细胞体外培养16 h后,通过免疫荧光染色检测纺锤体形态、ROS水平、线粒体分布和质量,利用qPCR检测抗氧化基因表达、线粒体呼吸链亚基的表达、整合应激反应相关基因的表达。结果表明:与对照组相比,130μmol/L Mic60抑制剂会降低卵母细胞体外成熟率(78.29%vs.21.08%,P<0.01),并显著阻碍纺锤体的组装;与对照组相比,Miclxin组卵母细胞中的ROS水平升高(P<0.05),且Sod1(0.83 vs. 0.22)和Sod2(0.84 vs. 0.27)基因表达降低(P<0.05),线粒体分布异常比例升高(0.16 vs. 0.57,P<0.05),线粒体膜电位水平降低(1.61 vs. 1.05,P<0.05),ATP产生降低(1.16 vs. 0.79,P<0.05);Miclxin组卵母细胞线粒体呼吸链复合体亚基Ndufv2和Sdha表达降低(P<0.05),Cyc1表达升高(P<0.05),卵母细胞mtDNA拷贝数降低(19 324 vs. 9 066,P<0.05);与对照组相比,Miclxin组卵母细胞Oma1、Dele1、Eif2ak1、eIF2α基因表达上调(P<0.05),触发了整合应激反应(Integrated Stress Response,ISR)并上调了ISR主要效应因子Atf4、Atf5和Chop的表达(P<0.05)。可见,抑制Mic60会通过损害线粒体功能并触发ISR影响卵母细胞体外成熟效果。展开更多
Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The ...Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).展开更多
This article described antimicrobial property and structure analysis of 2-hydroxypropane-1,2,3-tricarboxylic acid isolated from the crude extract of Citrus microcarpa. Presently, there was no report on compound from C...This article described antimicrobial property and structure analysis of 2-hydroxypropane-1,2,3-tricarboxylic acid isolated from the crude extract of Citrus microcarpa. Presently, there was no report on compound from C. microcarpa that possessed antimicrobial property against fish pathogenic bacteria. Therefore, in this study, the bioactive principle in C. microcarpa extract was isolated using thin layer chromatography. It＇s structure was elucidated based on nuclear magnetic resonance （NMR） spectroscopic data, such as proton NMR （1HNMR）, correlation spectroscopy, carbon 13 NMR, and heteronuclear multiple bond correlation data. This study showed that the bioactive compound isolated from C. microcarpa was 2-hydroxypropane-1,2,3-tricarboxylic acid monohydrate. The minimum inhibitory concentration （MIC） values of crude C. microcarpa extract and its bioactive component, 2-hydroxypropane-1,2,3-tricarboxylic acid as well as commercially available synthetic 2-hydroxypropane-1,2,3-tricarboxylic acid, were determined against 18 isolates of Edwardsiella tarda and 7 bacterial reference strains, namely, Escherichia coli （ATCC 25922）, Citrobacter freundii （ATCC 8090）, Aeromonas hydrophila （ATCC 49140）, Pseudomonas aeruginosa （ATCC 35032）, Streptococcus agalatiae （ATCC 13813）, E. tarda （ATCC 15947）, and Yersinia enterocolitica （ATCC 23715）, using two-fold microdilution method. The MIC values for both the natural 2-hydroxypropane-1,2,3-tricarboxylic acid and the synthetic one were ranging from 15.6 to 62.5 mg mL-1, whereas that of the crude extract was ranging from 7.8 to 31.3 mg mL-1. These findings showed that both the crude extract and its bioactive component might have potential as antimicrobial agent for aquaculture use.展开更多
[Objective] The paper was to investigate in vitro antibacterial effect of alkaline hydrogen water on Escherichia coli,Salmonella,Pseudomonas aeruginosa and Staphylococcus aureus.[Method] With Cortex cinnamomi extract...[Objective] The paper was to investigate in vitro antibacterial effect of alkaline hydrogen water on Escherichia coli,Salmonella,Pseudomonas aeruginosa and Staphylococcus aureus.[Method] With Cortex cinnamomi extract（1 g/m L） and pure water as the control,the minimal inhibitory concentrations（MIC） against four kinds of common pathogenic microorganisms were tested through microdilution method.[Result] When the alkaline hydrogen water was diluted to 1/8 times of the original concentration,it had significant antibacterial effects on four kinds of common mi-croorganisms with the concentration of 1.5 ×10^5 CFU/m L,which had equivalent effect with C.cinnamomi extract group.[Conclusion]The alkaline hydrogen water has remarkable antibacterial effects on the four kinds of common microorganisms,which may provide a new important way for pre-venting disease occurrence and reducing the harms of pathogenic microorganisms.展开更多
文摘本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母细胞体外培养16 h后,通过免疫荧光染色检测纺锤体形态、ROS水平、线粒体分布和质量,利用qPCR检测抗氧化基因表达、线粒体呼吸链亚基的表达、整合应激反应相关基因的表达。结果表明:与对照组相比,130μmol/L Mic60抑制剂会降低卵母细胞体外成熟率(78.29%vs.21.08%,P<0.01),并显著阻碍纺锤体的组装;与对照组相比,Miclxin组卵母细胞中的ROS水平升高(P<0.05),且Sod1(0.83 vs. 0.22)和Sod2(0.84 vs. 0.27)基因表达降低(P<0.05),线粒体分布异常比例升高(0.16 vs. 0.57,P<0.05),线粒体膜电位水平降低(1.61 vs. 1.05,P<0.05),ATP产生降低(1.16 vs. 0.79,P<0.05);Miclxin组卵母细胞线粒体呼吸链复合体亚基Ndufv2和Sdha表达降低(P<0.05),Cyc1表达升高(P<0.05),卵母细胞mtDNA拷贝数降低(19 324 vs. 9 066,P<0.05);与对照组相比,Miclxin组卵母细胞Oma1、Dele1、Eif2ak1、eIF2α基因表达上调(P<0.05),触发了整合应激反应(Integrated Stress Response,ISR)并上调了ISR主要效应因子Atf4、Atf5和Chop的表达(P<0.05)。可见,抑制Mic60会通过损害线粒体功能并触发ISR影响卵母细胞体外成熟效果。
基金Supported by Innovation Technology,Research and Development Project,Ministry of Science.Information and Communication Technology.Republic of Bangladesh Government(No.12)
文摘Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).
基金funded by E-Science Project (02-01-12-SF0055) provided by Ministry of Science, Technology and Innovation (MOSTI), Malaysia
文摘This article described antimicrobial property and structure analysis of 2-hydroxypropane-1,2,3-tricarboxylic acid isolated from the crude extract of Citrus microcarpa. Presently, there was no report on compound from C. microcarpa that possessed antimicrobial property against fish pathogenic bacteria. Therefore, in this study, the bioactive principle in C. microcarpa extract was isolated using thin layer chromatography. It＇s structure was elucidated based on nuclear magnetic resonance （NMR） spectroscopic data, such as proton NMR （1HNMR）, correlation spectroscopy, carbon 13 NMR, and heteronuclear multiple bond correlation data. This study showed that the bioactive compound isolated from C. microcarpa was 2-hydroxypropane-1,2,3-tricarboxylic acid monohydrate. The minimum inhibitory concentration （MIC） values of crude C. microcarpa extract and its bioactive component, 2-hydroxypropane-1,2,3-tricarboxylic acid as well as commercially available synthetic 2-hydroxypropane-1,2,3-tricarboxylic acid, were determined against 18 isolates of Edwardsiella tarda and 7 bacterial reference strains, namely, Escherichia coli （ATCC 25922）, Citrobacter freundii （ATCC 8090）, Aeromonas hydrophila （ATCC 49140）, Pseudomonas aeruginosa （ATCC 35032）, Streptococcus agalatiae （ATCC 13813）, E. tarda （ATCC 15947）, and Yersinia enterocolitica （ATCC 23715）, using two-fold microdilution method. The MIC values for both the natural 2-hydroxypropane-1,2,3-tricarboxylic acid and the synthetic one were ranging from 15.6 to 62.5 mg mL-1, whereas that of the crude extract was ranging from 7.8 to 31.3 mg mL-1. These findings showed that both the crude extract and its bioactive component might have potential as antimicrobial agent for aquaculture use.
文摘[Objective] The paper was to investigate in vitro antibacterial effect of alkaline hydrogen water on Escherichia coli,Salmonella,Pseudomonas aeruginosa and Staphylococcus aureus.[Method] With Cortex cinnamomi extract（1 g/m L） and pure water as the control,the minimal inhibitory concentrations（MIC） against four kinds of common pathogenic microorganisms were tested through microdilution method.[Result] When the alkaline hydrogen water was diluted to 1/8 times of the original concentration,it had significant antibacterial effects on four kinds of common mi-croorganisms with the concentration of 1.5 ×10^5 CFU/m L,which had equivalent effect with C.cinnamomi extract group.[Conclusion]The alkaline hydrogen water has remarkable antibacterial effects on the four kinds of common microorganisms,which may provide a new important way for pre-venting disease occurrence and reducing the harms of pathogenic microorganisms.
