This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plant...This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration(organ type and organ genesis type),methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.展开更多
Seeds of Elaeagnus mollis(Elaeagnaceae) produce an edible oil and contain more vitamin E(Ve) than major oil-seed crops. Despite its economic value, there is no information on its genome sequence. Here, we used the Ill...Seeds of Elaeagnus mollis(Elaeagnaceae) produce an edible oil and contain more vitamin E(Ve) than major oil-seed crops. Despite its economic value, there is no information on its genome sequence. Here, we used the Illumina platform to determine the seed transcriptome of E.mollis to identify the genes related to Ve biosynthesis and potential simple sequence repeat(SSR) markers. In total, 100,999 unigenes were obtained with an average length of 605 bp and N50 of 985 bp. Of these unigenes,52,256(51.7%) were annotated in at least one public database(NT, NR, PFAM, Swiss Prot, KOG, KO, and GO)in searches using blastn/x. The unigene annotation identified 15 unigenes encoding six enzymes(GGR, HPPD,HPT/VTE2, MPBQ-MT/VTE3, TC/VTE1, and c-TMT/VTE4) putatively involved in Ve biosynthesis. In addition,16,810 SSRs distributed in 14,057 unigenes were mined.Of these, 2820, 583, and 3423 SSRs were located in the 50–UTR, coding sequence(CDS), and 30–UTR regions,respectively, while the remaining 9984 SSRs had undetermined physical locations. The largest group of repeat motifs comprised mononucleotide repeats(70.76%), followed by dinucleotide(15.59%) and trinucleotide(12.10%)repeats. AG/CT(8.69%) and AAG/CTT(4.15%) were the main dinucleotide and trinucleotide repeats, respectively.Furthermore, 9597 SSR-specific primer pairs were designed. Among 100 primer pairs selected randomly to determine their usefulness, 53 proved to be efficient. To the our best of knowledge, this work is the first study of the E. mollis transcriptome and constitutes valuable genomics data for future genetic engineering studies to alter the amount of Ve. The identified potential EST-SSR markers can be used for population genetics studies and assistedbreeding of E. mollis.展开更多
Leymus mollis,a wild relative of wheat,is very tolerant to salt stress,and has been considered as a valuable genetic resource for wheat breeding.However,the genetic basis for salt tolerance of this species is still la...Leymus mollis,a wild relative of wheat,is very tolerant to salt stress,and has been considered as a valuable genetic resource for wheat breeding.However,the genetic basis for salt tolerance of this species is still largely unknown.In this study,de novo sequencing,assembly and analysis of L.mollis transcriptome in response to salt stress was performed.A total of 110,323 and 112,846 unigenes were generated for the NaCl-free(CK)and 180 mM NaCl-treated(CT)library,respectively.For the two libraries,73,414 unigenes were successfully annotated in five common protein databases,and 7521 differentially expressed genes(DEGs)between CK and CT libraries were identified.GO enrichment analysis of the DEGs showed that the significantly enriched GO terms were predominantly involved in environmental adaptation(including“response to abiotic stimulus”,“response to water deprivation”),regulation of signaling pathway(such as“regulation of abscisic acid mediated signaling pathway”,“regulation of cell communication”),and photosynthesis(including“response to light stimulus”,“photosynthesis,light harvesting”and“chlorophyll metabolic process”).KEGG pathway enrichment analysis showed that“mRNA surveillance pathway”,“RNA transport”and“plant hormone signal transduction”were predominantly enriched pathways,followed by several secondary metabolic pathways,photosynthesis,carbohydrate metabolism and lipid metabolism.In addition,DEGs related to osmotic stress,ion homeostasis and oxidative stress,including four dehydrins,five aquaporins,an LmNHX2 and several antioxidant enzymes or proteins genes,were found to be up-regulated in response to salt stress.These results will be helpful for further studies on the molecular mechanisms of salt responses in L.mollis.展开更多
A new oxoaporphine alkaloid, 1,2-methylene-dioxy-8-hydroxy-5- oxoaporphine, was isolated from the stem barks of Alphonsea mollis . Its structure was established on the basis of spectral analysis and chemical correlation.
Fifty-six compounds have been isolated from Abrus mollis until now,including saponins,steroids,flavonoids and fatty acid,which have the functions of promoting blood circulation to remove blood stasis,protecting the li...Fifty-six compounds have been isolated from Abrus mollis until now,including saponins,steroids,flavonoids and fatty acid,which have the functions of promoting blood circulation to remove blood stasis,protecting the liver and promoting gallbladder,anti-oxidation and enhancing immunity,etc.A.mollis has good therapeutic effects on liver diseases.In this paper,the chemical constituents and pharmacological effects of A.mollis were reviewed in order to provide a reference for the further development.展开更多
In different periods, the contents of nitrogen, ammonia nitrogen and nitrate nitrogen in different parts of Abrus mollis Hance were determined for each period by nesslerization, ninhydrin method and salicylic acid met...In different periods, the contents of nitrogen, ammonia nitrogen and nitrate nitrogen in different parts of Abrus mollis Hance were determined for each period by nesslerization, ninhydrin method and salicylic acid method in pot experiment. By using related data statistical software, the effects of Rhizobium inoculation on nitrogen metabolism, accumulation and distribution in Abrus mollis Hance were analyzed preliminarily.展开更多
[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group ...[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group ( cyclophosphamide group ), negative control group ( physiological saline group), high-dose A. moles Hance group (30 g/kg), moderate-dose A. mollis Hance group (20 g/kg) and low-dose A. mollis Hance group (10 g/kg). Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular cells were analyzed by using single cell gel electrophoresis assay, to investigate the effect of A. mollis Hance on DNA in mouse cells. [Result] Compared with positive control group, Tail DNA% and Tail Moment of moose liver, kidney, lung and testicular cells in A. moles Hance groups were significantly lower ( P 〈 0.01 ). Compared with negative control group, Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular ceils in high-dose A. mollis Hance group were significantly lower ( P 〈 0.01 ), while the other A. mollis Hance groups showed no statistically significant difference ( P 〉0.05 ). [ Conclusion] A. mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range.展开更多
In this study, polyamide fabrics were dyed with aqueous extracts from Diospyros Mollis fruits. The aqueous extracts from Diospyros Mollis fruits were extracted with water solution at room temperature and a ratio of 1...In this study, polyamide fabrics were dyed with aqueous extracts from Diospyros Mollis fruits. The aqueous extracts from Diospyros Mollis fruits were extracted with water solution at room temperature and a ratio of 1:5 for Diospyros Mollis to water. The influences of temperature in range of 50-100 ℃, time in range of 30-80 minutes and a ratio of aqueous extracts to water in range of 3/1-1/11 were investigated. The results showed that the aqueous extracts from Diospyros Mollis fruits could dye polyamide fabrics to have black color with color strength and fastness properties to be very good level of 4-5.展开更多
This study was carried out to assess the growth characteristics of Grewia moll&, Grewia tenax and Grewia villosa under the nursery and field conditions. Two experiments were conducted at the farm of the College of Na...This study was carried out to assess the growth characteristics of Grewia moll&, Grewia tenax and Grewia villosa under the nursery and field conditions. Two experiments were conducted at the farm of the College of Natural Resources and Environmental Studies, University of Juba, Khartoum, Sudan. Randomized complete block design with three replications was used. Morphological and physiological factors were measured. Seedlings height, number of leaves, number of branches and sub-branches were different (P 〈 0.05) among the three species at the nursery stage and under field conditions. Collar diameter showed significant difference among the species under field conditions. Physiological factors exhibited more significant variations in the field than at the nursery stage. Variations in growth characteristics were attributed to genetics differences and different growth habit, while variations in physiological factors (photosynthesis and transpiration rate) were attributed to differences in leaf structure, size and number of stomatal pores.展开更多
The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear...The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear edema model in mice and carrageenan-induced paw edema model in rats were applied. The hepatoprotective effects of AME were evaluated with various in vivo models of acute and chronic liver injury, including carbon tetrachloride(CCl4)-induced hepatitis in mice, D-galactosamine(D-GalN)-induced hepatitis in rats, as well as CCl4-induced hepatic fibrosis in rats. In the acute inflammation experiment, AME significantly suppressed xylene-induced ear edema and carrageenan-induced paw edema, respectively. In the acute hepatitis tests, AME significantly attenuated the excessive release of ALT and AST induced by CCl4 and D-GalN. In CCl4-induced hepatic fibrosis model, AME alleviated liver injury induced by CCl4 shown by histopathological sections of livers and improved liver function as indicated by decreased liver index, serum ALT, AST, TBIL, and ALP levels and hydroxyproline contents in liver tissues, and increased serum ALB and GLU levels. These results indicated that AME possesses potent anti-inflammatory activity in acute inflammation models and hepatoprotective activity in both acute and chronic liver injury models. In conclusion, AME is a potential anti-inflammatory and hepatoprotective agent and a viable candidate for treating inflammation, hepatitis, and hepatic fibrosis.展开更多
Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many potentially valuable traits that could be transferred to common wheat during breeding programs. In this study, the ...Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many potentially valuable traits that could be transferred to common wheat during breeding programs. In this study, the karyotypic constitution of a wheat - L. mollis 3D(3Ns#1) disomic substitution line isolated from the F5 progeny of octoploid Tritileymus M842-16 x Triticum durum cv. D4286, which was designated as 10DM57, was determined using genomic in situ hybridization (GISH), fluorescent in situ hybridization (FISH), SSR markers, and EST- STS markers. Screening of mitosis and meiosis showed that 10DM57 had a chromosome karyotype of 2n = 42 =21Ⅱ. GISH indicated that 10DM57 was a line with 40 chromosomes from wheat and two of the Ns chromosomes from L. mollis, which formed a ring bivalent in pollen mother cells at metaphase I. FISH analysis showed that the chromosome 3D may be replaced by 3Ns#1 in 10DM57. DNA markers, including SSR and EST-STS primers, showed that the pair of wheat chromosome 3D in 10DM57 was substituted by the pair of chromosome 3Ns#t from L. mollis. Evaluation of the agronomic traits showed that, compared with its common wheat relative 7182, 10DM57 was resistant to leaf rust while the spike length and number of spikes per plant were improved significantly, which correlated with a higher wheat yield. The new germplasm, 10DM57, could be exploited as an intermediate material in wheat genetic and breeding programs.展开更多
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust r...Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust resistance due to the subsequent emergence of the new races CYR30, CYR31, CYR32, and CYR33 (Han et al., 2010). Therefore, it is necessary to seek effective resistance genes and develop new resistance germ- plasm for wheat resistance breeding.展开更多
Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphat...Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphate precursors.Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity,but they remain largely unappreciated.Here,an extremely promiscuous terpenoid synthase(CcTPS1)of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var.mollis.CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom,accepting C_(25)/C_(20)/C_(15)/C_(10) diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids.Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids(two sesterterpenoids and a diterpenoid)with rare cyclohexanecontaining skeletons,along with four sesquiterpenoids and one monoterpenoid.Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy.Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids,demonstrating the enzyme’s promiscuity in planta.Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C.coccinea var.mollis and N.benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants.CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate,suggesting their possible role in plant–environment interaction.CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance.These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity;they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants.展开更多
A new species,Synanthedon elaeagnus sp.nov.feeding on Elaeagnus mollis,is described from Shanxi,China.This new species is similar to Synanthedon ulmicola Yang&Wang,1989 in general morphology,but it differs from th...A new species,Synanthedon elaeagnus sp.nov.feeding on Elaeagnus mollis,is described from Shanxi,China.This new species is similar to Synanthedon ulmicola Yang&Wang,1989 in general morphology,but it differs from this latter species by features of the forewing,female and male genitalia.Neighbor-joining and maximum likelihood analysis both recover Synanthedon elaeagnus sp.nov.as a well-supported monophyletic lineage with a high support value based on COI gene sequences.The key features of this new species are illustrated.展开更多
This study was carried out to assess the leaves and fruits of Grewia mollis, Grewia tenax and Grewia villosa for their potentials to produce high nutritive value fodder under semi-arid conditions. The experiment was c...This study was carried out to assess the leaves and fruits of Grewia mollis, Grewia tenax and Grewia villosa for their potentials to produce high nutritive value fodder under semi-arid conditions. The experiment was conducted at the farm of the College of Natural Resources and Environmental Studies, University of Juba-Khartoum-Sudan. Randomized Complete Block design with three replications was used. Proximate analysis was carried to estimate nutritive value, and mineral contents of leaves, seeds and fruit pulp of the three species. Results indicated significant difference (P ≤ 0.05) in nutritive value of the leaves among the three species; dry matter, ash, and acid detergent factor (ADF) kg/ha, while no significant difference was observed among the species in crude protein (CP) and nutrient detergent factor (NDF) kg/ha. Mineral contents of the leaves of the three species showed variations among the species and seasons. Seeds and fruit pulp were found to have considerable levels of nutrients and mineral contents. The nutritive value of the three species was higher in the rainy season compared to the dry season. The results of this study indicate that the three species can be introduced as a source of fodder in animal production farms and silvopastural systems.展开更多
Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determi...Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry.For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods:diphenyl-1-picrylhydrazyl(DPPH),2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)andβ-Carotene bleaching test.The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method.Results:The scavenging capacity of the radicals was assessed with DPPH and ABTS methods,the best results were found in the oils of M.mollis IC_(50) DPPH 2.80 mg/ml and IC_(50) ABTS 0.205 mg/mL and in A.glutinosa IC_(50) DPPH 12.972 mg/mL and IC_(50) ABTS 0.321 mg/mL,the results were compared with a pattern of natural reference in this case,the essential oil of T.vulgaris IC_(50) DPPH 0.474 mg/mL and IC_(50) ABTS 0.272 mg/mL.The evaluation of the antioxidant activity was determined by theβ-carotene bleaching test,the most notable activity results were from M.mollis IC_(50)0.119 mg/mL,A.glutinosa IC_(50)0.062 mg/mL and B.latifolia IC_(50)0,064 mg/mL.DPPH bioautography revealed the active molecules antioxidants in oils for M.mollis were thymol acetate(7.73%)and carvacrol acetate(24.52%),for A.glutinosa wasγ-muurolene(2.68%),and for B.latifolia Z-caryophyllene(2.99%),aristolochene(0.11%)and cis-cadin-4-en-7-ol(4.11%).Conclusion:The results of antioxidant activity shown in descending order that the essential oils of:M.mollis,A.glutinosa and B.latifolia,are those with the highest activity using the DPPH and ABTS methods.Theβ-Carotene bleaching test method confirms the 3 oils as the most active in the following order:A.glutinosa,B.latifolia and M.mollis.An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.展开更多
基金Supported by Scientific and Technological Development Project of Education Department in Shanxi(606048)~~
文摘This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration(organ type and organ genesis type),methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.
基金supported by the National Natural Science Foundation of China(31600549)Doctor Faculty Inaugurating Project of Northwest A&F University(2452015296)。
文摘Seeds of Elaeagnus mollis(Elaeagnaceae) produce an edible oil and contain more vitamin E(Ve) than major oil-seed crops. Despite its economic value, there is no information on its genome sequence. Here, we used the Illumina platform to determine the seed transcriptome of E.mollis to identify the genes related to Ve biosynthesis and potential simple sequence repeat(SSR) markers. In total, 100,999 unigenes were obtained with an average length of 605 bp and N50 of 985 bp. Of these unigenes,52,256(51.7%) were annotated in at least one public database(NT, NR, PFAM, Swiss Prot, KOG, KO, and GO)in searches using blastn/x. The unigene annotation identified 15 unigenes encoding six enzymes(GGR, HPPD,HPT/VTE2, MPBQ-MT/VTE3, TC/VTE1, and c-TMT/VTE4) putatively involved in Ve biosynthesis. In addition,16,810 SSRs distributed in 14,057 unigenes were mined.Of these, 2820, 583, and 3423 SSRs were located in the 50–UTR, coding sequence(CDS), and 30–UTR regions,respectively, while the remaining 9984 SSRs had undetermined physical locations. The largest group of repeat motifs comprised mononucleotide repeats(70.76%), followed by dinucleotide(15.59%) and trinucleotide(12.10%)repeats. AG/CT(8.69%) and AAG/CTT(4.15%) were the main dinucleotide and trinucleotide repeats, respectively.Furthermore, 9597 SSR-specific primer pairs were designed. Among 100 primer pairs selected randomly to determine their usefulness, 53 proved to be efficient. To the our best of knowledge, this work is the first study of the E. mollis transcriptome and constitutes valuable genomics data for future genetic engineering studies to alter the amount of Ve. The identified potential EST-SSR markers can be used for population genetics studies and assistedbreeding of E. mollis.
基金the National Natural Science Foundation of China(31400226)the Key Research and Development Program of Shandong Province(2019GSF110015)+1 种基金the Project of Shandong Province Higher Educational Science and Technology Program(J14LE07)the Key Research and Development Program of Yantai,Shandong Province(2018XSCC043)。
文摘Leymus mollis,a wild relative of wheat,is very tolerant to salt stress,and has been considered as a valuable genetic resource for wheat breeding.However,the genetic basis for salt tolerance of this species is still largely unknown.In this study,de novo sequencing,assembly and analysis of L.mollis transcriptome in response to salt stress was performed.A total of 110,323 and 112,846 unigenes were generated for the NaCl-free(CK)and 180 mM NaCl-treated(CT)library,respectively.For the two libraries,73,414 unigenes were successfully annotated in five common protein databases,and 7521 differentially expressed genes(DEGs)between CK and CT libraries were identified.GO enrichment analysis of the DEGs showed that the significantly enriched GO terms were predominantly involved in environmental adaptation(including“response to abiotic stimulus”,“response to water deprivation”),regulation of signaling pathway(such as“regulation of abscisic acid mediated signaling pathway”,“regulation of cell communication”),and photosynthesis(including“response to light stimulus”,“photosynthesis,light harvesting”and“chlorophyll metabolic process”).KEGG pathway enrichment analysis showed that“mRNA surveillance pathway”,“RNA transport”and“plant hormone signal transduction”were predominantly enriched pathways,followed by several secondary metabolic pathways,photosynthesis,carbohydrate metabolism and lipid metabolism.In addition,DEGs related to osmotic stress,ion homeostasis and oxidative stress,including four dehydrins,five aquaporins,an LmNHX2 and several antioxidant enzymes or proteins genes,were found to be up-regulated in response to salt stress.These results will be helpful for further studies on the molecular mechanisms of salt responses in L.mollis.
文摘A new oxoaporphine alkaloid, 1,2-methylene-dioxy-8-hydroxy-5- oxoaporphine, was isolated from the stem barks of Alphonsea mollis . Its structure was established on the basis of spectral analysis and chemical correlation.
文摘Fifty-six compounds have been isolated from Abrus mollis until now,including saponins,steroids,flavonoids and fatty acid,which have the functions of promoting blood circulation to remove blood stasis,protecting the liver and promoting gallbladder,anti-oxidation and enhancing immunity,etc.A.mollis has good therapeutic effects on liver diseases.In this paper,the chemical constituents and pharmacological effects of A.mollis were reviewed in order to provide a reference for the further development.
文摘In different periods, the contents of nitrogen, ammonia nitrogen and nitrate nitrogen in different parts of Abrus mollis Hance were determined for each period by nesslerization, ninhydrin method and salicylic acid method in pot experiment. By using related data statistical software, the effects of Rhizobium inoculation on nitrogen metabolism, accumulation and distribution in Abrus mollis Hance were analyzed preliminarily.
基金Supported by Scientific Research Project from Guangxi Department of Education(200710MS052)Project from Technology Bureau of Yulin City(0881038)
文摘[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group ( cyclophosphamide group ), negative control group ( physiological saline group), high-dose A. moles Hance group (30 g/kg), moderate-dose A. mollis Hance group (20 g/kg) and low-dose A. mollis Hance group (10 g/kg). Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular cells were analyzed by using single cell gel electrophoresis assay, to investigate the effect of A. mollis Hance on DNA in mouse cells. [Result] Compared with positive control group, Tail DNA% and Tail Moment of moose liver, kidney, lung and testicular cells in A. moles Hance groups were significantly lower ( P 〈 0.01 ). Compared with negative control group, Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular ceils in high-dose A. mollis Hance group were significantly lower ( P 〈 0.01 ), while the other A. mollis Hance groups showed no statistically significant difference ( P 〉0.05 ). [ Conclusion] A. mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range.
文摘In this study, polyamide fabrics were dyed with aqueous extracts from Diospyros Mollis fruits. The aqueous extracts from Diospyros Mollis fruits were extracted with water solution at room temperature and a ratio of 1:5 for Diospyros Mollis to water. The influences of temperature in range of 50-100 ℃, time in range of 30-80 minutes and a ratio of aqueous extracts to water in range of 3/1-1/11 were investigated. The results showed that the aqueous extracts from Diospyros Mollis fruits could dye polyamide fabrics to have black color with color strength and fastness properties to be very good level of 4-5.
文摘This study was carried out to assess the growth characteristics of Grewia moll&, Grewia tenax and Grewia villosa under the nursery and field conditions. Two experiments were conducted at the farm of the College of Natural Resources and Environmental Studies, University of Juba, Khartoum, Sudan. Randomized complete block design with three replications was used. Morphological and physiological factors were measured. Seedlings height, number of leaves, number of branches and sub-branches were different (P 〈 0.05) among the three species at the nursery stage and under field conditions. Collar diameter showed significant difference among the species under field conditions. Physiological factors exhibited more significant variations in the field than at the nursery stage. Variations in growth characteristics were attributed to genetics differences and different growth habit, while variations in physiological factors (photosynthesis and transpiration rate) were attributed to differences in leaf structure, size and number of stomatal pores.
基金supported by Mega-projects of Science Research for the 11th Five-Year Plan(No.2009ZX 09103-315)12th Five-Year Plan(No.2013ZX09301-303-003)+1 种基金the 111 Project(No.111-2-07)2011’Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education
文摘The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear edema model in mice and carrageenan-induced paw edema model in rats were applied. The hepatoprotective effects of AME were evaluated with various in vivo models of acute and chronic liver injury, including carbon tetrachloride(CCl4)-induced hepatitis in mice, D-galactosamine(D-GalN)-induced hepatitis in rats, as well as CCl4-induced hepatic fibrosis in rats. In the acute inflammation experiment, AME significantly suppressed xylene-induced ear edema and carrageenan-induced paw edema, respectively. In the acute hepatitis tests, AME significantly attenuated the excessive release of ALT and AST induced by CCl4 and D-GalN. In CCl4-induced hepatic fibrosis model, AME alleviated liver injury induced by CCl4 shown by histopathological sections of livers and improved liver function as indicated by decreased liver index, serum ALT, AST, TBIL, and ALP levels and hydroxyproline contents in liver tissues, and increased serum ALB and GLU levels. These results indicated that AME possesses potent anti-inflammatory activity in acute inflammation models and hepatoprotective activity in both acute and chronic liver injury models. In conclusion, AME is a potential anti-inflammatory and hepatoprotective agent and a viable candidate for treating inflammation, hepatitis, and hepatic fibrosis.
基金supported by the National High Technology Research and Development Program of China (863 Program) (Grant No.2011AA100501)the Major Innovation Project for Science and Technology of Shaanxi Province (Grant No.2011KTZB02-01)+1 种基金the Basic Scientific Research Project in Central Universities (Grant No.QN2011001)the Tang Zhong-Ying Breeding Funding Project at the Northwest A&F University in China
文摘Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many potentially valuable traits that could be transferred to common wheat during breeding programs. In this study, the karyotypic constitution of a wheat - L. mollis 3D(3Ns#1) disomic substitution line isolated from the F5 progeny of octoploid Tritileymus M842-16 x Triticum durum cv. D4286, which was designated as 10DM57, was determined using genomic in situ hybridization (GISH), fluorescent in situ hybridization (FISH), SSR markers, and EST- STS markers. Screening of mitosis and meiosis showed that 10DM57 had a chromosome karyotype of 2n = 42 =21Ⅱ. GISH indicated that 10DM57 was a line with 40 chromosomes from wheat and two of the Ns chromosomes from L. mollis, which formed a ring bivalent in pollen mother cells at metaphase I. FISH analysis showed that the chromosome 3D may be replaced by 3Ns#1 in 10DM57. DNA markers, including SSR and EST-STS primers, showed that the pair of wheat chromosome 3D in 10DM57 was substituted by the pair of chromosome 3Ns#t from L. mollis. Evaluation of the agronomic traits showed that, compared with its common wheat relative 7182, 10DM57 was resistant to leaf rust while the spike length and number of spikes per plant were improved significantly, which correlated with a higher wheat yield. The new germplasm, 10DM57, could be exploited as an intermediate material in wheat genetic and breeding programs.
基金supported by the grants from the National High Technology Research and Development Program of China (No. 2011AA100102)the Chinese Academy of Sciences (No. KSCX2-EW-N-02)
文摘Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust resistance due to the subsequent emergence of the new races CYR30, CYR31, CYR32, and CYR33 (Han et al., 2010). Therefore, it is necessary to seek effective resistance genes and develop new resistance germ- plasm for wheat resistance breeding.
基金supported financially by the National Natural Science Foundation of China(21937006,31770390,31770340 and 31525005)the Yunnan Key Research and Development Program(no.2019ZF011-2)+1 种基金the Science Foundation of Yunnan(2018FA017)the Youth Innovation Promotion association and“Western Light”Program of the CAS(to Y.Liu).
文摘Terpenoids are the largest class of natural products with complex structures and extensive bioactivities;their scaffolds are generated by diverse terpenoid synthases(TPSs)from a limited number of isoprenoid diphosphate precursors.Promiscuous TPSs play important roles in the evolution of terpenoid chemodiversity,but they remain largely unappreciated.Here,an extremely promiscuous terpenoid synthase(CcTPS1)of the TPS-b subfamily was cloned and functionally characterized from a leaf-specific transcriptome of the Lamiaceae plant Colquhounia coccinea var.mollis.CcTPS1 is the first sester-/di-/sesqui-/mono-TPS identified from the plant kingdom,accepting C_(25)/C_(20)/C_(15)/C_(10) diphosphate substrates to generate a panel of sester-/di-/sesqui-/mono-terpenoids.Engineered Escherichia coli expressing CcTPS1 produced three previously unreported terpenoids(two sesterterpenoids and a diterpenoid)with rare cyclohexanecontaining skeletons,along with four sesquiterpenoids and one monoterpenoid.Their structures were elucidated by extensive nuclear magnetic resonance spectroscopy.Nicotiana benthamiana transiently expressing CcTPS1 also produced the diterpenoid and sesquiterpenoids,demonstrating the enzyme’s promiscuity in planta.Its highly leaf-specific expression pattern combined with detectable terpenoid products in leaves of C.coccinea var.mollis and N.benthamiana expressing CcTPS1 suggested that CcTPS1 was mainly responsible for diterpenoid and sesquiterpenoid biosynthesis in plants.CcTPS1 expression and the terpenoid products could be induced by methyl jasmonate,suggesting their possible role in plant–environment interaction.CcTPS1 was localized to the cytosol and may differ from mono-TPSs in subcellular compartmentalization and substrate tolerance.These findings will greatly aid our understanding of plant TPS evolution and terpenoid chemodiversity;they also highlight the enormous potential of transcriptome mining and heterologous expression for the exploration of unique enzymes and natural products hidden in plants.
基金supported by the National Natural Science Foundation of China(31772508)。
文摘A new species,Synanthedon elaeagnus sp.nov.feeding on Elaeagnus mollis,is described from Shanxi,China.This new species is similar to Synanthedon ulmicola Yang&Wang,1989 in general morphology,but it differs from this latter species by features of the forewing,female and male genitalia.Neighbor-joining and maximum likelihood analysis both recover Synanthedon elaeagnus sp.nov.as a well-supported monophyletic lineage with a high support value based on COI gene sequences.The key features of this new species are illustrated.
文摘This study was carried out to assess the leaves and fruits of Grewia mollis, Grewia tenax and Grewia villosa for their potentials to produce high nutritive value fodder under semi-arid conditions. The experiment was conducted at the farm of the College of Natural Resources and Environmental Studies, University of Juba-Khartoum-Sudan. Randomized Complete Block design with three replications was used. Proximate analysis was carried to estimate nutritive value, and mineral contents of leaves, seeds and fruit pulp of the three species. Results indicated significant difference (P ≤ 0.05) in nutritive value of the leaves among the three species; dry matter, ash, and acid detergent factor (ADF) kg/ha, while no significant difference was observed among the species in crude protein (CP) and nutrient detergent factor (NDF) kg/ha. Mineral contents of the leaves of the three species showed variations among the species and seasons. Seeds and fruit pulp were found to have considerable levels of nutrients and mineral contents. The nutritive value of the three species was higher in the rainy season compared to the dry season. The results of this study indicate that the three species can be introduced as a source of fodder in animal production farms and silvopastural systems.
文摘Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry.For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods:diphenyl-1-picrylhydrazyl(DPPH),2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)andβ-Carotene bleaching test.The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method.Results:The scavenging capacity of the radicals was assessed with DPPH and ABTS methods,the best results were found in the oils of M.mollis IC_(50) DPPH 2.80 mg/ml and IC_(50) ABTS 0.205 mg/mL and in A.glutinosa IC_(50) DPPH 12.972 mg/mL and IC_(50) ABTS 0.321 mg/mL,the results were compared with a pattern of natural reference in this case,the essential oil of T.vulgaris IC_(50) DPPH 0.474 mg/mL and IC_(50) ABTS 0.272 mg/mL.The evaluation of the antioxidant activity was determined by theβ-carotene bleaching test,the most notable activity results were from M.mollis IC_(50)0.119 mg/mL,A.glutinosa IC_(50)0.062 mg/mL and B.latifolia IC_(50)0,064 mg/mL.DPPH bioautography revealed the active molecules antioxidants in oils for M.mollis were thymol acetate(7.73%)and carvacrol acetate(24.52%),for A.glutinosa wasγ-muurolene(2.68%),and for B.latifolia Z-caryophyllene(2.99%),aristolochene(0.11%)and cis-cadin-4-en-7-ol(4.11%).Conclusion:The results of antioxidant activity shown in descending order that the essential oils of:M.mollis,A.glutinosa and B.latifolia,are those with the highest activity using the DPPH and ABTS methods.Theβ-Carotene bleaching test method confirms the 3 oils as the most active in the following order:A.glutinosa,B.latifolia and M.mollis.An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.