期刊文献+
共找到182篇文章
< 1 2 10 >
每页显示 20 50 100
根癌农杆菌胁迫下多花蔷薇(Rosamultiflora‘Inermis4’)的基因表达分析 被引量:8
1
作者 赵小兰 苏晓华 +1 位作者 田传卫 赵梁军 《中国农业科学》 CAS CSCD 北大核心 2005年第7期1425-1430,共6页
多花蔷薇无刺4号(Rosamultiflora‘Inermis4’)对根癌病有较强抗性。以根癌农杆菌(Agrobacteriumtumefaciens)接种多花蔷薇无刺4号为目的材料,刺伤处理为对照,采用抑制差减杂交(suppressionsubtractivehybridization,SSH)方法,构建了富... 多花蔷薇无刺4号(Rosamultiflora‘Inermis4’)对根癌病有较强抗性。以根癌农杆菌(Agrobacteriumtumefaciens)接种多花蔷薇无刺4号为目的材料,刺伤处理为对照,采用抑制差减杂交(suppressionsubtractivehybridization,SSH)方法,构建了富集多花蔷薇根癌病抗性相关基因的差减cDNA文库。差异筛选差减cDNA文库后,随机选取增强表达的92个克隆进行测序,去除冗余的cDNA和病菌的基因片段,共得到53个单一序列。利用GenBank数据库进行核酸和蛋白质同源性比较,获得的cDNA片段功能涉及:细胞分裂、生长(22个克隆),逆境防御、信号转导及激素调节(11个克隆)以及初生与次生代谢(10个克隆)。另有10个基因片段功能未知,可能为新的EST。根据测序及比对分析结果,对多花蔷薇无刺4号抗根癌病的分子机理进行了探讨。 展开更多
关键词 根癌农杆菌 基因表达分析 蔷薇 差减CDNA文库 GENBANK数据库 胁迫 抑制差减杂交 cDNA片段 基因片段 同源性比较 根癌病 相关基因 差异筛选 细胞分裂 次生代谢 激素调节 信号转导 分析结果 分子机理 克隆 蛋白质 EST 抗性
下载PDF
多花山竹子(Garcinia multiflora Champ)的繁殖研究 被引量:5
2
作者 刘胜洪 黄碧珠 《中国农学通报》 CSCD 2004年第6期93-95,共3页
对多花山竹子枝条插穗基部采用不同的切制方法和不同的激素水平处理,种子分剥皮和不剥皮两组进行不同激素水平处理后进行播种。研究表明:多花山竹子的扦插繁殖在各种条件下均较难成活;而种子在人工剥皮后用BA(50μl/L)处理后的繁殖效果... 对多花山竹子枝条插穗基部采用不同的切制方法和不同的激素水平处理,种子分剥皮和不剥皮两组进行不同激素水平处理后进行播种。研究表明:多花山竹子的扦插繁殖在各种条件下均较难成活;而种子在人工剥皮后用BA(50μl/L)处理后的繁殖效果最好。 展开更多
关键词 多花山竹子 激素 扦插繁殖 种子繁殖 人工剥皮 道路绿化树种
下载PDF
部分蔷薇(Rosa multiflora)属植物的RAPD分析 被引量:1
3
作者 管晓庆 王奎玲 +3 位作者 刘庆华 刘庆超 师长海 唐启和 《中国农学通报》 CSCD 2008年第7期328-331,共4页
蔷薇作为园林观赏植物,在中国城市绿化中应用较为广泛,但目前应用的蔷薇品种较为混杂,尚无系统研究。本研究采用RAPD技术对部分蔷薇属植物进行亲缘关系的分析,旨在进一步明确蔷薇种、品种,为引种育种以及材料选择提供参考。对蔷薇属植物... 蔷薇作为园林观赏植物,在中国城市绿化中应用较为广泛,但目前应用的蔷薇品种较为混杂,尚无系统研究。本研究采用RAPD技术对部分蔷薇属植物进行亲缘关系的分析,旨在进一步明确蔷薇种、品种,为引种育种以及材料选择提供参考。对蔷薇属植物的2个种、1个变种、20个品种进行了RAPD分析,16个引物对供试蔷薇材料PCR扩增获得175条谱带,其中多态性条带151条(86.3%)表现出丰富的遗传多态性。且不同花色系蔷薇属植物间的遗传多态性不同,白色系75.16%,粉红色系73.46%,黄色系37.50%。利用UPGMA法构建树状聚类图,蔷薇属植物的2个种、1个变种、20个品种在阈值为0.667处被分划为两大类群,这两大类群间的遗传距离较近,黄蔷薇与黄刺枚单独被划分为一个类群,与上述两类群间的遗传距离较远。聚类结果显示出了蔷薇的演化趋势:白色、单瓣是蔷薇较为原始的一个性状,花径大、重瓣性高是现代蔷薇栽培品种的性状,而部分粉红色半重瓣蔷薇则是从野生种到现代栽培品种的中间过渡类型。 展开更多
关键词 蔷薇 RAPD 遗传多态性 聚类分析
下载PDF
A Genetic Transformation System for Rosa multiflora Thunb. var. cathayensis Rehd. et Wils through Callus Induction 被引量:1
4
作者 张冬梅 姜灵敏 +1 位作者 罗玉兰 覃仁娟 《Agricultural Science & Technology》 CAS 2014年第1期1-5,共5页
An efficient genetic transformation system is a preparation for Rosa multi-flora Thunb. var. cathayensis Rehd. et Wils to diversify its flower color through ge-netic engineering. We firstly optimized the explants and ... An efficient genetic transformation system is a preparation for Rosa multi-flora Thunb. var. cathayensis Rehd. et Wils to diversify its flower color through ge-netic engineering. We firstly optimized the explants and culture conditions on callus induction, hormone concentrations and dark period of culture time on bud differentia-tions in particular, with sterilized seedlings to establish the regeneration system of R. multiflora. It showed that callus induction frequency reached 100% after the ex-plants being cultured in dark for 21 d when MS was chosen to be the initial culture medium. The bud differentiation rate was 48% after cal i being cultured under dark for 8 d on MS medium supplemented with TDZ (1.5 mg/L) and NAA (0.05 mg/L). The cal i was used as the explants that were infected with Agrobacterium tumefa-ciens harboring a DFR-RNAi construct. The transformation rate reached as high as 50%. The establishment of a highly efficient rose gene transformation system out-lined in this report is prerequisite for genetic improvement in rose flower colors. 展开更多
关键词 Rosa multiflora Callus induction Callus proliferation Genelic transfor-mation system
下载PDF
Drought Resistance of Slope Pioneer Plant Magnolia multiflora 被引量:1
5
作者 许翩翩 王建柱 《Agricultural Science & Technology》 CAS 2016年第9期2037-2041,2045,共6页
[Objective] This study aimed to reveal responses of Magnolia multiflora to soil drought stress. [Method] Pot experiment was employed to simulate drought stress, and photosynthetic and physiological indices of M. multi... [Objective] This study aimed to reveal responses of Magnolia multiflora to soil drought stress. [Method] Pot experiment was employed to simulate drought stress, and photosynthetic and physiological indices of M. multiflora were determined. [Result] The net photosynthetic rate (Pn) of M. multiflora did not change significantly under mild drought stress, and reduced significantly under moderate and severe drought stress. Drought stress reduced stomatal conductance (Gs), transpiration rate (Tr) and intercellular CO2 concentration (Ci), and compared with those in the control group, the Pn, Gs, Tr and Ci under severe drought stress declined by 61.04%, 86.27%, 87.77% and 42.63%, respectively. The malondialdehyde (MDA) content in M. multiflora leaves did not increase significantly under mild drought stress, and increased significantly under moderate and severe drought stress. The MDA content in M. multiflora leaves under severe drought stress was 1.63 times as high as that in the control group. The proline (Pro) and soluble sugar contents of M. multiflora increased significantly with the aggravation of drought stress, and those under severe drought stress were 8.06 times and 3.16 times respectively higher than those in the control group. [Conclusion] M. multiflora has a strong drought tolerance, and is suitable for growing in relatively arid environment. It can be used as candidate for vegetation restoration in hydropower engineering slope. 展开更多
关键词 Hydropower engineering Drought stress Magnolia multiflora Photosynthetic characteristics Physiological characteristics
下载PDF
Antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss 被引量:5
6
作者 Mansour Amin Enayat Kalantar +1 位作者 Neda Mohammad-Saeid Behzad Ahsan 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第6期439-442,共4页
ObjectiveTo evaluate the antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss.MethodsAntibacterial activity of essential oil of Zataria multiflora Boiss was assessed by agar... ObjectiveTo evaluate the antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss.MethodsAntibacterial activity of essential oil of Zataria multiflora Boiss was assessed by agar disc diffusion and minimal inhibitory concentration(MIC) was tested by E test.ResultsThe essential oil of Zataria multiflora Boiss was effective on pathogenic bacteria particularly Staphylococcus aureus (S. aureus). The MIC values of the target cultures ranged from 0.39 mg/mL to 1.56 mg/mL. The physicochemical properties like effects of pH, temperature, detergents, and enzymes on the activity of essential oil from Zataria multiflora Boiss were also determined. The essential oil was quite stable to temperature as tested against S. aureus and Escherichia coli (E. coli). The essential oil was very stable over a wide range of pH. The antibacterial activity of essential oil was insensitive to various protein-denaturing detergents (Such as Tween 80, Tween 20, Triton 100, etc.) and enzymes (namely proteinase K, trypsin, lipase, and lysosyme).ConclusionsA potential use of the essential oil from Zataria multiflora Boiss is suggested. More studies including further purification, mass spectra, nuclear magnetic resonance (NMR) and evaluation of toxicity are needed for confirmation of this suggestion. 展开更多
关键词 Antibacterial effect Physico–chemical properties Zataria multiflora Boiss
下载PDF
In vivoand in vitroanti-arthritic efficacy of a herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos
7
作者 BrianChiYanCHENG HuiGUO +3 位作者 TaoSU Xiu-qiongFU TingLI Zhi-lingYU 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2015年第S1期59-60,共2页
OBJECTIVE Rheumatoid arthritis(RA)is the most common inflammatory autoimmune disease,affecting around 1% of the world population.Toll-like receptor 4(TLR4)signalling has been found to be involved in the pathogenesis o... OBJECTIVE Rheumatoid arthritis(RA)is the most common inflammatory autoimmune disease,affecting around 1% of the world population.Toll-like receptor 4(TLR4)signalling has been found to be involved in the pathogenesis of RA.It is a potential therapeutic target for RA treatment.A herbal formula(RL)consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos has traditionally been used in treating various inflammatory disorders.In this study,we would evaluate the anti-arthritic effect of RL on collagen-induced arthritis(CIA)in rats and investigate the involvement of TLR4 signaling in the mode of action of RL in vivo and in vitro.METHODS In vivo anti-arthritic efficacy was evaluated using CIA rats induced by bovine typeⅡ collagen.The treatment groups were treated with various concentrations of RL or positive control indomethacin for 35 d.Clinical signs(hind paw volume and arthritis severity scores),changes in serum inflammatory mediators,histological and radiographic changes of joints were investigated.Spleens and peritoneal macrophages were used to determine the effects of RL on innate and adaptive immune responses in CIA rats.The involvement of TLR4 signalling pathways in the anti-arthritic effect of RL was examined in cartilage tissue of CIA rats,murine RAW264.7macrophages and human THP-1 monocytic cells.RESULTS The severity of arthritis in the CIA rats was significantly attenuated by RL.Histological score and radiographic score were efficiently improved by RL.RL could also dose-dependently inhibit pro-inflammatory cytokines in serum of CIA rats.RL significantly inhibited the production of various pro-inflammatory mediators,the expression and/or activity of the components of TLR4 signalling pathways in animal tissue and cell lines.CONCLUSION RL possesses anti-arthritic effect on collagen-induced arthritis in rats.The therapeutic effect of RL may be related to its inhibition on pro-inflammatory cytokines in serum.The inhibition of the TLR4/TAK1/NF-κB and TLR4/TAK1/MAPK pathways participate in the anti-arthritic effects of RL.This provides a pharmacological justification for the use of RL in the control of various arthritic diseases.Further investigation should be done to develop RL into a modern anti-arthritic agent. 展开更多
关键词 Rosae multiflorae Fructus Lonicerae Japonicae Flos
下载PDF
Therapeutic potential of Zataria multiflora:A narrative review of current evidence
8
作者 Reza Nosratabadi Fatemeh Anaeigoudari Akbar Anaeigoudari 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2023年第11期451-459,共9页
Zataria multiflora Boiss is a perennial plant with a wide spectrum of biological and pharmacological activities including antidiabetic,antinociceptive,anti-asthmatic,anti-fever,anti-spastic,anti-oxidative,anti-inflamm... Zataria multiflora Boiss is a perennial plant with a wide spectrum of biological and pharmacological activities including antidiabetic,antinociceptive,anti-asthmatic,anti-fever,anti-spastic,anti-oxidative,anti-inflammatory,and antimicrobial properties.This paper reviews the therapeutic effects of Zataria multiflora based on recent reports.The relevant reports were extracted by checking the electronic databases including PubMed,Scopus,Web of Science,and Google Scholar from the beginning of 2010 until the end of May 2023.The neuroprotective effects of Zataria multiflora can be attributed to inhibition of acetylcholinesterase,enhancement of brain-derived neurotrophic factor,and alleviation of brain oxidative damage.Zataria multiflora also exerts its protective effects on the respiratory system,liver,and kidney by reducing the level of inflammatory cytokines,scavenging the free radicals,and augmenting the antioxidant enzymes.Additionally,Zataria multiflora accelerates wound healing via upregulating transforming growth factor-β,insulin-like growth factor 1,fibroblast growth factor 2,and vascular endothelial growth factor,and inducing angiogenesis and collagen biosynthesis.Overall,the protective impacts of Zataria multiflora on different organs are mainly attributed to its antioxidant and antiinflammatory properties. 展开更多
关键词 Zataria multiflora Anti-inflammmatory ANTIOXIDATIVE NEUROPROTECTIVE ORGANS
下载PDF
Zataria multiflora and its constituent,carvacrol,counteract sepsis-induced aortic and cardiac toxicity in rat:Involvement of nitric oxide and oxidative stress
9
作者 Mahmoud Hosseini Zohreh Arab +3 位作者 Farimah Beheshti Akbar Anaeigoudari Farzaneh Shakeri Arezoo Rajabian 《Animal Models and Experimental Medicine》 CAS CSCD 2023年第3期221-229,共9页
Background:Zataria multiflora and carvacrol showed various pharmacological prop-erties including anti-inflammatory and anti-oxidant effects.However,up to now no studies have explored its potential benefits in ameliora... Background:Zataria multiflora and carvacrol showed various pharmacological prop-erties including anti-inflammatory and anti-oxidant effects.However,up to now no studies have explored its potential benefits in ameliorating sepsis-induced aortic and cardiac injury.Thus,this study aimed to investigate the effects of Z.multiflora and carvacrol on nitric oxide(NO)and oxidative stress indicators in lipopolysaccharide(LPS)-induced aortic and cardiac injury.Methods:Adult male Wistar rats were assigned to:Control,lipopolysaccharide(LPS)(1 mg/kg,intraperitoneal(i.p.)),and Z.multiflora hydro-ethanolic extract(ZME,50–200 mg/kg,oral)-and carvacrol(25–100 mg/kg,oral)-treated groups.LPS was in-jected daily for 14 days.Treatment with ZME and carvacrol started 3 days before LPS administration and treatment continued during LPS administration.At the end of the study,the levels of malondialdehyde(MDA),NO,thiols,and antioxidant enzymes were evaluated.Results:Our findings showed a significant reduction in the levels of superoxide dis-mutase(SOD),catalase(CAT),and thiols in the LPS group,which were restored by ZME and carvacrol.Furthermore,ZME and carvacrol decreased MDA and NO in car-diac and aortic tissues of LPS-injected rats.Conclusions:The results suggest protective effects of ZME and carvacrol on LPS-induced cardiovascular injury via improved redox hemostasis and attenuated NO pro-duction.However,additional studies are needed to elucidate the effects of ZME and its constituents on inflammatory responses mediated by LPS. 展开更多
关键词 CARVACROL LIPOPOLYSACCHARIDE nitric oxide oxidative injury SEPSIS Z.multiflora
下载PDF
Determination of the Minerals of the Herbal Tea and Tea Green from Lippia multiflora
10
作者 Ekissi Alice Christine Yao-Kouamé Albert Kati-Coulibaly Séraphin 《American Journal of Plant Sciences》 2017年第11期2608-2621,共14页
This study aims to determine the mineral composition of green teas and herbal tea in the savannah tea plant. Samples of buds, young leaves and broadleaf stems were collected from wild plants of Lippia multiflora in th... This study aims to determine the mineral composition of green teas and herbal tea in the savannah tea plant. Samples of buds, young leaves and broadleaf stems were collected from wild plants of Lippia multiflora in three localities, including two from the Center (Yamoussoukro, Toumodi) and one from the northeast (Bondoukou) of C&#244;te d’Ivoire. The lipid and ash contents were carried out by the AOAC method. The total protein and sugar contents were determined by the Kjeldahl and Agbo methods, respectively. Nine minerals (potassium, calcium, magnesium, iron, sodium, manganese, zinc, copper and cadmium) were quantitated by Atomic Absorption Spectrophotometry (AAS) from green teas (buds and young leaves) and herbal tea of Lippia multiflora. The results showed that ash levels (7.05% - 13.94%) and protein (6.22% - 9.97%) were high, however the lipid contents (1.01% - 2.98%) and total sugars (1.43% - 4.51%) are low. Majority of the minerals are calcium (2320.91 to 36150.10 mg/kg), potassium (2110.71 to 12174.67 mg/kg) and magnesium (3620.87 - 5870.46 mg/kg). Iron (52.55 to 483.11 mg/kg), sodium (35.66 to 469.16 mg/kg) and manganese (15.25 to 76.86 mg/kg) are represented in average proportions. There are small amounts of zinc (9.61 - 47.50 mg/kg), copper (5.66 - 8.71 mg/kg), and cadmium (0.17 - 1.49 mg/kg). Lippia multiflora herbal teas and green teas are low in fat, high in ash and are good sources of protein and minerals. 展开更多
关键词 HERBAL TEA LIPPIA multiflora BUDS Young LEAVES Green TEA
下载PDF
Magnesium Enhances the Hepatorenal Protective Effects of Lippia multiflora Aqueous Leaves Extract in Streptozotocin-Diabetic Rats
11
作者 Yapo Fulgence Allo Viviane Jocelyne Zannou-Tchoko +2 位作者 Kouamé Hermann Yéboué Brou André Konan Jacques Yao Datté 《Journal of Biosciences and Medicines》 2023年第10期35-45,共11页
Purpose: This study was conducted in order to evaluate the antidiabetic effects of the aqueous extract of Lippia multiflora supplemented with magnesium on some biochemical markers of the kidneys and liver in type 2 di... Purpose: This study was conducted in order to evaluate the antidiabetic effects of the aqueous extract of Lippia multiflora supplemented with magnesium on some biochemical markers of the kidneys and liver in type 2 diabetic rats. Method: 7 groups of 4 STZ-diabetic rats received separately orally Glucophage<sup>®</sup> (Glu 10 mg/kg), the plant extract (LiMAE 200 - 600 mg/kg) and the plant extract supplemented with magnesium (LiMAE-Mg 200 - 600 mg/kg). After a daily treatment of 21 days, serum biochemical parameters were assayed in 16 hr-fasted rats. Results: Diabetes caused a significant (p Conclusion: The addition of magnesium to the extract of Lippia multiflora caused a greater reduction in the levels of urea, creatinine, ALT and AST increased in STZ-diabetic rats. Magnesium would therefore enhance the nephroprotective and hepatoprotective effects of Lippia multiflora in diabetic rats. 展开更多
关键词 Lippia multiflora Diabetes STREPTOZOTOCIN NEPHROPROTECTIVE HEPATOPROTECTIVE
下载PDF
Gas Chromatography-Based Fingerprinting and Chemical Pattern Recognition of Rosa multiflora Volatile Oil
12
作者 Tuniyazi Gulimeikereyi Ablajan Nurpida +1 位作者 Malike Dilinuer Hailaxi Alai 《Journal of Agricultural Science and Technology(A)》 2017年第1期31-38,共8页
In this paper, gas chromatography-mass spectrometry (GS-MS) was used to build the standard fingerprint of volatile oil from Rosa multiflora Thunb. from 12 different habitats. Fourteen components in the volatile oil ... In this paper, gas chromatography-mass spectrometry (GS-MS) was used to build the standard fingerprint of volatile oil from Rosa multiflora Thunb. from 12 different habitats. Fourteen components in the volatile oil were identified as the indicator components ofR. multiflora, of which one was selected as the standard. The GC analysis conditions used for fingerprinting afford a very good separating effect. The similarity of the 12 volatile oils from R. multiflora Thunb. was more than 0.84, and the precision, stability and repeatability of the fingerprints were quite good. It could be concluded that the fingerprints can be used as the standard and as a quality control method for medicinal materials from R. multiflora Thunb.. 展开更多
关键词 Rosa multiflora volatile oil GC-MS fingerprinting main component.
下载PDF
山竹子茎叶化学成分的研究
13
作者 焦志林 刘涛 +4 位作者 唐琍萍 杨玥 汪嘉诚 杨引霞 高雪梅 《中药材》 CAS 北大核心 2024年第3期629-633,共5页
目的:研究藤黄科藤黄属植物山竹子Garcinia multiflora Champ.ex Benth.茎叶的化学成分。方法:运用硅胶、HPLC等多种色谱技术对山竹子茎叶的醇提物进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果:从山竹子茎叶中分离得到1... 目的:研究藤黄科藤黄属植物山竹子Garcinia multiflora Champ.ex Benth.茎叶的化学成分。方法:运用硅胶、HPLC等多种色谱技术对山竹子茎叶的醇提物进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果:从山竹子茎叶中分离得到14个化合物,分别鉴定为:1,3,7-三羟基呫吨酮(1)、1,3,6,7-四羟基呫吨酮(2)、1,6-二羟基-3,5,7-三甲氧基呫吨酮(3)、GDPPH-1(4)、香橙素(5)、eriodictyol(6)、7,5,4′-三羟基黄酮(7)、4,6,4′-三羟基-2,3′-二甲氧基-3-异戊烯基二苯甲酮(8)、garcicowin D(9)、30-epi-cambogin(10)、garcimultiflorone H(11)、garcinol(12)、1-丙酮,1-(3,4-二羟基-5-甲氧基苯基)(13)、loliolide(14)。结论:其中,化合物1、4~6、9、11~14为首次从该植物中分离得到。 展开更多
关键词 山竹子 化学成分 呫吨酮类化合物 黄酮类化合物 苯甲酮类化合物
下载PDF
野蔷薇根总黄酮对类风湿关节炎模型大鼠血管损伤的改善作用及机制
14
作者 李开言 张飞 +5 位作者 田萍 杨丹 张薇 王晓丽 屠万倩 刘一菲 《中国药房》 CAS 北大核心 2024年第8期931-935,共5页
目的研究野蔷薇根总黄酮对类风湿关节炎(RA)模型大鼠血管损伤的改善作用及其潜在机制。方法将雌性Wistar大鼠随机分为正常对照组、模型组、阿司匹林组(阳性对照,30mg/kg)和野蔷薇根总黄酮低、高剂量组(4.15、8.30g/kg,以生药量计),每组1... 目的研究野蔷薇根总黄酮对类风湿关节炎(RA)模型大鼠血管损伤的改善作用及其潜在机制。方法将雌性Wistar大鼠随机分为正常对照组、模型组、阿司匹林组(阳性对照,30mg/kg)和野蔷薇根总黄酮低、高剂量组(4.15、8.30g/kg,以生药量计),每组10只。除正常对照组外,其余各组大鼠采用胶原诱导+高脂饮食喂养建立RA模型。造模14 d后,各组大鼠灌胃相应药液/0.5%羧甲基纤维素钠溶液,每天1次,连续36d。定期评估大鼠的全身评分、关节炎指数和足爪肿胀数;末次给药后,检测大鼠血清中白细胞介素6(IL-6)、细胞间黏附分子1(ICAM-1)和血管细胞黏附分子1(VCAM-1)水平,观察大鼠胸主动脉血管组织病理学形态变化,检测胸主动脉血管组织中Toll样受体4(TLR4)蛋白表达水平和Janus激酶2(JAK2)、信号转导及转录激活因子3(STAT3)蛋白磷酸化水平。结果与正常对照组比较,模型组大鼠血清中IL-6、ICAM-1、VCAM-1水平和胸主动脉血管组织中TLR4蛋白表达水平及JAK2、STAT3蛋白磷酸化水平均显著升高(P<0.01),胸主动脉血管组织可见动脉粥样斑块(粥瘤)、胆固醇结晶、淋巴细胞浸润及少量未破裂的泡沫细胞聚集。与模型组比较,在第28天时,野蔷薇根总黄酮各剂量组大鼠的全身评分(低剂量组除外)、关节炎指数和足爪肿胀数均显著降低(P<0.05或P<0.01);在第49天时,野蔷薇根总黄酮低剂量组大鼠的全身评分、关节炎指数和足爪肿胀数均显著降低(P<0.05或P<0.01);野蔷薇根总黄酮各剂量组大鼠血清和血管组织中其余定量指标均显著逆转(P<0.05或P<0.01),血管组织病理损伤明显减轻。结论野蔷薇根总黄酮可改善RA模型大鼠的血管损伤,其作用可能与降低血管组织中TLR4蛋白表达,抑制IL-6/JAK2/STAT3信号通路活性有关。 展开更多
关键词 野蔷薇根总黄酮 类风湿关节炎 炎症 血管损伤 TLR4 IL-6/JAK2/STAT3信号通路
下载PDF
多花蔷薇总RNA提取方法 被引量:11
15
作者 赵小兰 苏晓华 赵梁军 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第9期89-93,共5页
根据总RNA完整性、纯度和得率筛选出适合多花蔷薇幼嫩根、叶总RNA的提取方法.结果表明,以CTAB/酸酚法提取的扦插苗根系总RNA、以LiCl-尿素法提取的扦插苗根、叶总RNA以及采用RNeasy Plant Mini Kit试剂盒的改进方法提取的组培苗嫩叶总RN... 根据总RNA完整性、纯度和得率筛选出适合多花蔷薇幼嫩根、叶总RNA的提取方法.结果表明,以CTAB/酸酚法提取的扦插苗根系总RNA、以LiCl-尿素法提取的扦插苗根、叶总RNA以及采用RNeasy Plant Mini Kit试剂盒的改进方法提取的组培苗嫩叶总RNA电泳有清晰明亮的28S、18S条带,无降解;其A260/A280值为1.73~2.04,表明总RNA质量好.RT-PCR结果进一步证实所提取的总RNA能够用于分子生物学的各种下游实验.RNA得率分别为:根系和组培苗嫩叶120-140μg/g(fw),扦插苗嫩叶190-230μg/g(fw).CTAB/酸酚法提取的嫩叶总RNA、SDS/酸酚法提取的根、叶总RNA有多糖污染,且有明显降解.Total RNA isolation system(Z5111,Promega)试剂盒不适合提取多花蔷薇各组织总RNA. 展开更多
关键词 多花蔷薇(Rosa multiflora) RNA提取 LiCl-尿素法 CTAB/酸酚法 RNA提取方法 蔷薇 组织总RNA CTAB Plant
下载PDF
多花蔷薇假珠芽诱导、体细胞胚发生及植株高效再生 被引量:23
16
作者 田传卫 尚爱芹 +3 位作者 张建甫 郭艳超 梁建丽 赵梁军 《园艺学报》 CAS CSCD 北大核心 2008年第3期403-408,共6页
以多花蔷薇‘无刺3号’成熟种子为外植体,探讨了假珠芽诱导、体细胞胚发生及植株高效再生的方法。结果表明,种子的子叶在添加2,4-D0.5-2mg·L^-1的1/2MS培养基上暗培养30d后所产生的愈伤组织,接种到添加TDZ5-20mg·L^-1的1/2MS... 以多花蔷薇‘无刺3号’成熟种子为外植体,探讨了假珠芽诱导、体细胞胚发生及植株高效再生的方法。结果表明,种子的子叶在添加2,4-D0.5-2mg·L^-1的1/2MS培养基上暗培养30d后所产生的愈伤组织,接种到添加TDZ5-20mg·L^-1的1/2MS培养基上光培养20d产生初级假珠芽。假珠芽在添加TDZ10mg·L^-1和GA30.1mg·L^-1的诱导培养基上暗培养20d后,在含麦芽糖的无植物生长调节剂的培养基上光培养产生少量次级假珠芽和胚性愈伤组织。假珠芽保存在诱导培养基上。胚性愈伤组织可发育成大量正常体细胞胚,继而再生正常植物。假珠芽可通过上述方法不断诱导体细胞胚和假珠芽,通过这种方法假珠芽已经保存了2年。 展开更多
关键词 多花蔷薇 假珠芽 体细胞胚 植株再生
下载PDF
微波消解-火焰原子吸收法测定新疆野蔷薇果中金属元素 被引量:24
17
作者 李茵萍 关明 +2 位作者 杜为军 刘从 迪丽努尔.马里克 《食品科学》 EI CAS CSCD 北大核心 2010年第8期143-145,共3页
采用HNO3消解体系对野蔷薇果进行程序升温微波消解制样,应用火焰原子吸收法测定野蔷薇果中的Ca、K、Mn、Zn、Mg、Fe、Cu、Na、Cr、Ni、Sr共11种金属元素的含量。对样品前处理条件进行筛选,确定样品适合的微波消解体系并进行消化结果的... 采用HNO3消解体系对野蔷薇果进行程序升温微波消解制样,应用火焰原子吸收法测定野蔷薇果中的Ca、K、Mn、Zn、Mg、Fe、Cu、Na、Cr、Ni、Sr共11种金属元素的含量。对样品前处理条件进行筛选,确定样品适合的微波消解体系并进行消化结果的准确度和精密度实验。该方法的加标回收率为96.50%~104.50%,相对标准偏差(RSD)≤3.21%。结果表明,新疆野蔷薇果中富含Ca、Mg、K、Na、Mn、Fe等对人体有益的金属元素,而Cr、Ni等金属元素的含量较低。 展开更多
关键词 火焰原子吸收法 微波消解 野蔷薇果 微量元素
下载PDF
何首乌乙酸乙酯提取物对秀丽线虫的抗衰老作用研究 被引量:10
18
作者 唐进法 张帆 +4 位作者 李宇辉 吴宿慧 李寒冰 李伟霞 李学林 《中国药房》 CAS 北大核心 2017年第4期493-496,共4页
目的:研究何首乌乙酸乙酯提取物(EPM)对秀丽线虫(简称"线虫")寿命的影响,考察EPM抗衰老作用。方法:设立EPM低、中、高浓度组(25、37.5、50 mg/L,以生药计)和空白组(0 mg/L),考察EPM对线虫寿命的影响;设立EPM组(37.5 mg/L)和... 目的:研究何首乌乙酸乙酯提取物(EPM)对秀丽线虫(简称"线虫")寿命的影响,考察EPM抗衰老作用。方法:设立EPM低、中、高浓度组(25、37.5、50 mg/L,以生药计)和空白组(0 mg/L),考察EPM对线虫寿命的影响;设立EPM组(37.5 mg/L)和空白组(0 mg/L),分别进行线虫的生殖实验、急性热应激实验和急性氧化应激实验,考察EPM对线虫生殖能力以及压力应激的影响。结果:在寿命实验中,EPM低、中、高浓度组线虫的平均寿命较空白组均显著延长(P<0.05或P<0.01),以中浓度EPM作用最为明显;增殖实验中,EPM组线虫在第2、3天中的子代数量较空白组显著增加(P<0.05);在急性热应激实验和急性氧化应激实验中,EPM组线虫的平均生存时间较空白组明显延长(P<0.05)。结论:37.5 mg/L的EPM能延缓线虫衰老且不损害其生殖能力。 展开更多
关键词 何首乌 乙酸乙酯提取物 抗衰老 秀丽线虫 寿命 氧化应激 热应激
下载PDF
何首乌及其常见混淆品的matK基因序列分析及鉴别 被引量:12
19
作者 生书晶 严萍 +1 位作者 郑传进 赵树进 《中药材》 CAS CSCD 北大核心 2010年第11期1707-1711,共5页
目的:比较何首乌与其常见混淆品的matK基因序列,从分子水平对中药材何首乌进行鉴定。方法:改良的CTAB法提取基因组DNA,用一对matK通用引物进行PCR扩增,TA克隆后进行测序和序列分析。结果:除毛脉蓼之外,其他混淆品与正品何首乌间的matK... 目的:比较何首乌与其常见混淆品的matK基因序列,从分子水平对中药材何首乌进行鉴定。方法:改良的CTAB法提取基因组DNA,用一对matK通用引物进行PCR扩增,TA克隆后进行测序和序列分析。结果:除毛脉蓼之外,其他混淆品与正品何首乌间的matK基因序列差异较大,不论是核苷酸替代数还是遗传距离,都远远大于不同居群的何首乌之间;进一步比对分析,发现了可以快速鉴别何首乌及其同属混淆品的鉴别位点;运用matK序列分析成功地对3种市售何首乌药材进行了真伪鉴定。结论:根据matK序列特征,能有效区分何首乌及其常见混淆品,matK序列可以作为何首乌药材鉴定的分子标记。 展开更多
关键词 何首乌 MATK基因 分子鉴定 混淆品
下载PDF
4种新疆植物提取物对人肝癌细胞生长抑制作用的研究 被引量:14
20
作者 孙建新 朱虎虎 +2 位作者 肖辉 连军 安娟 《新疆医科大学学报》 CAS 2008年第7期828-830,共3页
目的:研究新疆地产植物野蔷薇、黑加仑、沙枣、巴旦杏提取物对人肝癌细胞的抑制作用。方法:将野蔷薇、黑加仑、沙枣、巴旦杏分别分为高、中、低剂量组(10-1、10-2、10-3g/ml),另设阳性(顺铂)对照组、阴性对照组(含同一细胞密度的培养液... 目的:研究新疆地产植物野蔷薇、黑加仑、沙枣、巴旦杏提取物对人肝癌细胞的抑制作用。方法:将野蔷薇、黑加仑、沙枣、巴旦杏分别分为高、中、低剂量组(10-1、10-2、10-3g/ml),另设阳性(顺铂)对照组、阴性对照组(含同一细胞密度的培养液)、溶剂对照组(7.5%甘油),在人肝癌细胞中加入不同剂量浓度的受试物,分别于24、48、72 h在倒置显微镜下观察肿瘤细胞的生长情况,通过MTT比色法测定OD值。结果:与阴性对照组比较,野蔷薇高剂量和黑加仑72 h、沙枣和巴旦杏48 h对肿瘤细胞QCY-7404的抑制作用最强,野蔷薇和黑加仑的抑瘤作用随时间的延长而加强,而沙枣和巴旦杏的抑瘤作用与时间没有依赖关系。结论:野蔷薇、黑加仑、沙枣和巴旦杏提取物高剂量对肿瘤细胞QCY-7704有明显的抑制作用。 展开更多
关键词 野蔷薇 黑加仑 沙枣 巴旦杏 抗肿瘤
下载PDF
上一页 1 2 10 下一页 到第
使用帮助 返回顶部