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Multilineage Differentiation of Dental Pulp Stem Cells from Green Fluorescent Protein Transgenic Mice 被引量:5
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作者 Brian E.Grottkau P.Prasad Purudappa 《International Journal of Oral Science》 SCIE CAS CSCD 2010年第1期21-27,共7页
Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also obs... Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also observed during differentiation. Methodology DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR. Results The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation. Conclusion As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo. 展开更多
关键词 dental pulp stem cells multilineage differentiation green fluorescent protein
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Adipose tissue in bone regeneration-stem cell source and beyond 被引量:1
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作者 Luminita Labusca 《World Journal of Stem Cells》 SCIE 2022年第6期372-392,共21页
Adipose tissue(AT)is recognized as a complex organ involved in major homeostatic body functions,such as food intake,energy balance,immunomodulation,development and growth,and functioning of the reproductive organs.The... Adipose tissue(AT)is recognized as a complex organ involved in major homeostatic body functions,such as food intake,energy balance,immunomodulation,development and growth,and functioning of the reproductive organs.The role of AT in tissue and organ homeostasis,repair and regeneration is increasingly recognized.Different AT compartments(white AT,brown AT and bone marrow AT)and their interrelation with bone metabolism will be presented.AT-derived stem cell populations-adipose-derived mesenchymal stem cells and pluripotentlike stem cells.Multilineage differentiating stress-enduring and dedifferentiated fat cells can be obtained in relatively high quantities compared to other sources.Their role in different strategies of bone and fracture healing tissue engineering and cell therapy will be described.The current use of AT-or AT-derived stem cell populations for fracture healing and bone regenerative strategies will be presented,as well as major challenges in furthering bone regenerative strategies to clinical settings. 展开更多
关键词 Adipose tissue Bone metabolism Fracture healing Adipose-derived stem cells multilineage differentiating stress-enduring Dedifferentiated fat cells Bone engineering
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Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method
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作者 Myung-Shin Jeon Tac-Ghee Yi +6 位作者 Hyun-Ja Lim Sun-Hwa Moon Moon-Hee Lee Joon-Soon Kang Chul-Soo Kim Dae-Hyun Lee Sun U Song 《World Journal of Stem Cells》 SCIE CAS 2011年第8期70-82,共13页
AIM:To characterize single-cell-derived mouse clonal mesenchymal stem cells (mcMSCs) established with bone marrow samples from three different mouse strains. METHODS:We established mcMSC lines using subfractionation c... AIM:To characterize single-cell-derived mouse clonal mesenchymal stem cells (mcMSCs) established with bone marrow samples from three different mouse strains. METHODS:We established mcMSC lines using subfractionation culturing method from bone marrow samples obtained from long bones.These lines were characterized by measuring cell growth, cell surface epitopes, differentiation potential, lineage-specific gene expression and T-cell suppression capability. Nonclonal MSCs isolated by the conventional gradient centrifugation method were used as controls. RESULTS:All mcMSC lines showed typical nonclonal MSC-like spindle shape morphology. Lines differed inoptimal growth density requirement.Cell surface epitope prof iles of these mcMSC lines were similar to those of nonclonal MSCs. However, some lines exhibited different expression levels in a few epitopes, such as CD44 and CD105. Differentiation assays showed that 90% of the mcMSC lines were capable of differentiating into adipogenic and/or chondrogenic lineages, but only 20% showed osteogenic lineage differentiation. T-cell suppression analysis showed that 75% of the lines exhibited T-cell suppression capability. CONCLUSION:mcMSC lines have similar cell morphology and cell growth rate but exhibit variations in their cell surface epitopes, differentiation potential, lineage-specifi c gene expression and T-cell suppression capability. 展开更多
关键词 CLONAL STEM CELLS Bone MARROW STEM CELLS multilineage differentiation CLONAL STROMAL cell isolation T-CELL suppression
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The testis:an accessible Mesenchymal Stem Cells source
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作者 Myret Ghabriel Asma Amleh 《Advances in Modern Oncology Research》 2018年第6期5-17,共13页
Mesenchymal stem cells(MSCs)are adult stem cells that are predominantly found in bone marrow but also cord blood,peripheral blood,lungs,and adipose tissue.They are considered multipotent cells,which can differentiate ... Mesenchymal stem cells(MSCs)are adult stem cells that are predominantly found in bone marrow but also cord blood,peripheral blood,lungs,and adipose tissue.They are considered multipotent cells,which can differentiate to form multiple cells types—adipocytes,bone,cartilage,skin,and muscle.This potential elicits a great deal of attention because it appears to hold great promise regarding regenerative cell-based therapy to treat various diseases,including cancer.They are considered powerful allogeneic nonhematopoietic transplantation stem cells due to their low immunogenicity.Recently,several researchers have identified and isolated MSCs from primary human testes cultures.This review aimed to highlight the latest advances in MSC biological properties and characteristics studies,focusing on testicular mesenchymal stromal stem cells(tMSC),their differentiation capacity,and immunosuppressive properties—all of which have the potential to establish MSCs as powerful therapeutic agents for various diseases,including cancer. 展开更多
关键词 TESTICULAR MESENCHYMAL STROMAL stem cells MSC sources MSC ISOLATION multilineage potential THERAPEUTIC application
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Efficient expansion of rare human circulating hematopoietic stem/progenitor cells in steady-state blood using a polypeptide-forming 3D culture 被引量:1
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作者 Yulin Xu Xiangjun Zeng +17 位作者 Mingming Zhang Binsheng Wang Xin Guo Wei Shan Shuyang Cai Qian Luo Honghu Li Xia Li Xue Li Hao Zhang Limengmeng Wang Yu Lin Lizhen Liu Yanwei Li Meng Zhang Xiaohong Yu Pengxu Qian He Huang 《Protein & Cell》 SCIE CSCD 2022年第11期808-824,共17页
Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be u... Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be used as an alternative source remains largely elusive.Here we develop a three-dimensional culture system(3DCS)including arginine,glycine,aspartate,and a series of factors.Fourteen-day culture of peripheral blood mononuclear cells(PBMNCs)in 3DCS led to 125-and 70-fold increase of the frequency and number of CD34+cells.Further,3DCS-expanded cHSPCs exhibited the similar reconstitution rate com-pared to CD34+HSPCs in bone marrow.Mechanistically,3DCS fabricated an immunomodulatory niche,secreting cytokines as TNF to support cHSPC survival and proliferation.Finally,3DCS could also promote the expansion of cHSPCs in patients who failed in HSPC mobilization.Our 3DCS successfully expands rare cHSPCs,providing an alternative source for the HSPC therapy,particularly for the patients/donors who have failed in HSPC mobilization. 展开更多
关键词 hematopoietic stem/progenitor cell transplantation peripheral blood mononuclear cells EXPANSION MOBILIZATION three-dimensional culture selfrenewal and multilineage differentiation
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