Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging

The miniaturized femtosecond laser in near infrared-Ⅱregion is the core equipment of threephoton microscopy.In this paper,we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy.Based on an all-polarizationmaintaining passive mode-locked fiber laser,we shift the center wavelength of the pulses to the 1.7m band utilizing cascade Raman effect,thereby generate dual-wavelength pulses.To enhance clarity,the two wavelengths are separated through the graded-index multimode fiber.Then we obtain the dual-pulse sequences with 1639.4 nm and 1683.7 nm wavelengths,920 fs pulse duration,and 23.75 MHz pulse repetition rate.The average power of the signal is 53.64mW,corresponding to a single pulse energy of 2.25 nJ.This illumination source can be further amplified and compressed for three-photon fluorescence imaging,especially dual-color three-photon fluorescence imaging,making it an ideal option for biomedical applications.

基于表面等离子体共振的微位移光纤传感器

为实现对微小位移的精确测量,提高位移精度,提出了基于表面等离子体共振的微位移光纤传感器。利用渐变折射率多模光纤中光束的传播角度随入射光位置的变化而变化的特性,结合表面等离子体共振传感器的共振波长对共振角度变化具有很高灵敏度的特性,实现对微小位移的精确测量。为满足共振条件,将渐变折射率多模光纤研磨成具有合适角度的楔形,精确控制渐变折射率多模光纤的长度,并将光纤探针浸入到一定折射率的液体中。通过630nm单模光纤将白光光源从渐变折射率多模光纤的端面耦合到光纤探针中,搭建位移平台,精确控制单模光纤和渐变折射率多模光纤的径向相对位置,通过光谱仪检测共振波长随相对位置的变化规律。实验结果表明:当光纤研磨角度为12°,且液体折射率为1.350时,该传感器具有高达10.32nm·μm-1的灵敏度,位移分辨率高达1.9nm。