[ Objective] The paper was to screen myxobacteria with antifungal activity, and identify its species. [ Method] 237 myxobacteria were isolated and pu- rified from 200 copies of soil samples collected from 23 provinces...[ Objective] The paper was to screen myxobacteria with antifungal activity, and identify its species. [ Method] 237 myxobacteria were isolated and pu- rified from 200 copies of soil samples collected from 23 provinces in China. The strains were fermented in four different media, and the fermentation products were extracted by methanol to obtain metabolites. With Candida albicans as the model, the secondary metabolites were carried out high-throughput screening. [ Result ] A strain of myxohacteria numbered ZJ2 was obtained after secondary screening, its secondary metabolites had activities against G. albicans. 16S rDNA cloning and sequencing results showed that ZJ2 was Myxococcus fidvus. [ Conclusion ] The secondary metabolites of ZJ2 strain had bioactive substances with significant inhibi- tion effect against the growth of C. albicans, which had potential pharmaceutical research and development value.展开更多
This paper reported that the authors isolated large numbers of myxobacteria from more than 100 samples collected in many places of China.More than 400 pure strains were obtained.These strains belonged to 10 genera of ...This paper reported that the authors isolated large numbers of myxobacteria from more than 100 samples collected in many places of China.More than 400 pure strains were obtained.These strains belonged to 10 genera of Myxococcales.The most frequent genera isolated were Myxococcus,Sorangium,Corallococcus, and Cystobacter.Melittangium was seldom isolated.No Chondromyces and Haploangium were found.展开更多
[ Objective] To lay the basis for further studying bioactive substances in myxobacteria. [ Method] A total of 35 samples including fresh water, mud, rabbit dung, sheep dung, weathered rock and rotten wood were collect...[ Objective] To lay the basis for further studying bioactive substances in myxobacteria. [ Method] A total of 35 samples including fresh water, mud, rabbit dung, sheep dung, weathered rock and rotten wood were collected from districts and counties of Chengdu. After pretreatment, the myxobacteria were isolated and purified using different methods. Vegetative cells, myxospores, fruiting body and colony morphology of these myxobacteria strains were observed. E ResultJ Forty-two myxobacteda strains were isolated and purified. They were classified into seven genera including Myxococcus, Angiococcus, Chondromyces, Sorangium, Melittangium, Stigmatella and Archangium. The plating efficiency of myxobacteria was highest in the fresh water samples. [ Conclusion] Abundant myxobacteria resource is found in fresh water.展开更多
Myxobacteria are famous for their capacity for social behavior and natural product biosynthesis.The unique sociality of myxobacteria is not only an intriguing scientific topic but also the main limiting factor for the...Myxobacteria are famous for their capacity for social behavior and natural product biosynthesis.The unique sociality of myxobacteria is not only an intriguing scientific topic but also the main limiting factor for their ma-nipulation.After more than half a century of research,a series of genetic techniques for myxobacteria have been developed,rendering these mysterious bacteria manipulable.Here,we review the advances in genetic manipu-lation of myxobacteria,with a particular focus on the exploitation of secondary metabolism.We emphasize the necessity and urgency of constructing the myxobacterial chassis for synthetic biology research and the exploita-tion of untapped secondary metabolism.展开更多
采用大肠杆菌诱导法和滤纸诱导法对广西北部湾不同海洋生境采集的样品进行黏细菌的分离纯化,基于菌落形态特征结合16S r RNA基因序列分析对黏细菌纯培养物进行初步鉴定。从21份海洋生境样品中共分离纯化得到38株黏细菌,隶属于4属10种,...采用大肠杆菌诱导法和滤纸诱导法对广西北部湾不同海洋生境采集的样品进行黏细菌的分离纯化,基于菌落形态特征结合16S r RNA基因序列分析对黏细菌纯培养物进行初步鉴定。从21份海洋生境样品中共分离纯化得到38株黏细菌,隶属于4属10种,其中黏球菌属和珊瑚球菌属分别占菌株总数的63.16%和26.32%,属于优势菌属。采用VY/2培养基进行固体培养发酵,利用丙酮提取浓缩获得提取物,进一步利用滤纸片琼脂扩散法对菌株粗提物进行抗菌活性初步筛选。结果发现24株黏细菌提取物对9种指示菌具有不同程度的抑制作用,抗菌阳性率达63.16%,其中Myxococcus macrosporus(1H01)的提取物对肺炎克雷伯菌的抑制活性显著,优于阳性药(氨苄青霉素钠,1mg·mL^(-1))。研究结果表明广西北部湾特殊海洋生境黏细菌多样性丰富,分离出的黏细菌具有良好的抗菌活性,为新型抗生素应用研究奠定了物质基础。展开更多
基金Supported by Agriculture Public Relation Projects of Science and Technology Department of Shaanxi Province"Utilization of Specific Biological Resources and New Product Development in Southern Shaanxi"(2009K01-25)Industrial Pilot Projects of Education Department of Shaanxi Province"Industrializa-tion of Additives of Green Pollution-free Microbial Fermentation Feed"(2010JC22)~~
文摘[ Objective] The paper was to screen myxobacteria with antifungal activity, and identify its species. [ Method] 237 myxobacteria were isolated and pu- rified from 200 copies of soil samples collected from 23 provinces in China. The strains were fermented in four different media, and the fermentation products were extracted by methanol to obtain metabolites. With Candida albicans as the model, the secondary metabolites were carried out high-throughput screening. [ Result ] A strain of myxohacteria numbered ZJ2 was obtained after secondary screening, its secondary metabolites had activities against G. albicans. 16S rDNA cloning and sequencing results showed that ZJ2 was Myxococcus fidvus. [ Conclusion ] The secondary metabolites of ZJ2 strain had bioactive substances with significant inhibi- tion effect against the growth of C. albicans, which had potential pharmaceutical research and development value.
文摘This paper reported that the authors isolated large numbers of myxobacteria from more than 100 samples collected in many places of China.More than 400 pure strains were obtained.These strains belonged to 10 genera of Myxococcales.The most frequent genera isolated were Myxococcus,Sorangium,Corallococcus, and Cystobacter.Melittangium was seldom isolated.No Chondromyces and Haploangium were found.
基金Supported by Scientific Research Fund of Sichuan Provincial Education Department (08zb092)School Fund of Chengdu University (2009XJZ16 and 2010XJZ32)
文摘[ Objective] To lay the basis for further studying bioactive substances in myxobacteria. [ Method] A total of 35 samples including fresh water, mud, rabbit dung, sheep dung, weathered rock and rotten wood were collected from districts and counties of Chengdu. After pretreatment, the myxobacteria were isolated and purified using different methods. Vegetative cells, myxospores, fruiting body and colony morphology of these myxobacteria strains were observed. E ResultJ Forty-two myxobacteda strains were isolated and purified. They were classified into seven genera including Myxococcus, Angiococcus, Chondromyces, Sorangium, Melittangium, Stigmatella and Archangium. The plating efficiency of myxobacteria was highest in the fresh water samples. [ Conclusion] Abundant myxobacteria resource is found in fresh water.
基金This work was financially supported by the National Key Re-search and Development Programs of China(2018YFA0900400,2018YFA0901704 and 2021YFC2101000)the Natural Science Foundation of Shandong Province(ZR2019BC041).
文摘Myxobacteria are famous for their capacity for social behavior and natural product biosynthesis.The unique sociality of myxobacteria is not only an intriguing scientific topic but also the main limiting factor for their ma-nipulation.After more than half a century of research,a series of genetic techniques for myxobacteria have been developed,rendering these mysterious bacteria manipulable.Here,we review the advances in genetic manipu-lation of myxobacteria,with a particular focus on the exploitation of secondary metabolism.We emphasize the necessity and urgency of constructing the myxobacterial chassis for synthetic biology research and the exploita-tion of untapped secondary metabolism.
文摘采用大肠杆菌诱导法和滤纸诱导法对广西北部湾不同海洋生境采集的样品进行黏细菌的分离纯化,基于菌落形态特征结合16S r RNA基因序列分析对黏细菌纯培养物进行初步鉴定。从21份海洋生境样品中共分离纯化得到38株黏细菌,隶属于4属10种,其中黏球菌属和珊瑚球菌属分别占菌株总数的63.16%和26.32%,属于优势菌属。采用VY/2培养基进行固体培养发酵,利用丙酮提取浓缩获得提取物,进一步利用滤纸片琼脂扩散法对菌株粗提物进行抗菌活性初步筛选。结果发现24株黏细菌提取物对9种指示菌具有不同程度的抑制作用,抗菌阳性率达63.16%,其中Myxococcus macrosporus(1H01)的提取物对肺炎克雷伯菌的抑制活性显著,优于阳性药(氨苄青霉素钠,1mg·mL^(-1))。研究结果表明广西北部湾特殊海洋生境黏细菌多样性丰富,分离出的黏细菌具有良好的抗菌活性,为新型抗生素应用研究奠定了物质基础。
