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Olive Leaf Disease Detection via Wavelet Transform and Feature Fusion of Pre-Trained Deep Learning Models
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作者 Mahmood A.Mahmood Khalaf Alsalem 《Computers, Materials & Continua》 SCIE EI 2024年第3期3431-3448,共18页
Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wa... Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wavelet,feature-fused,pre-trained deep learning model for detecting olive leaf diseases.The proposed model combines wavelet transforms with pre-trained deep-learning models to extract discriminative features from olive leaf images.The model has four main phases:preprocessing using data augmentation,three-level wavelet transformation,learning using pre-trained deep learning models,and a fused deep learning model.In the preprocessing phase,the image dataset is augmented using techniques such as resizing,rescaling,flipping,rotation,zooming,and contrasting.In wavelet transformation,the augmented images are decomposed into three frequency levels.Three pre-trained deep learning models,EfficientNet-B7,DenseNet-201,and ResNet-152-V2,are used in the learning phase.The models were trained using the approximate images of the third-level sub-band of the wavelet transform.In the fused phase,the fused model consists of a merge layer,three dense layers,and two dropout layers.The proposed model was evaluated using a dataset of images of healthy and infected olive leaves.It achieved an accuracy of 99.72%in the diagnosis of olive leaf diseases,which exceeds the accuracy of other methods reported in the literature.This finding suggests that our proposed method is a promising tool for the early detection of olive leaf diseases. 展开更多
关键词 olive leaf diseases wavelet transform deep learning feature fusion
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Protective effects of oleic acid and polyphenols in extra virgin olive oil on cardiovascular diseases
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作者 Yan Lu Jun Zhao +6 位作者 Qiqi Xin Rong Yuan Yu Miao Manli Yang Hui Mo Keji Chen Weihong Cong 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期529-540,共12页
The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which... The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which have been shown to have noteworthy nutritional value and positive impact on human health.It is worth noting that EVOO owes its superior nutritional value to its bioactive composition.The main component of EVOO is monounsaturated fatty acids(MUFAs)in the form of oleic acid.Oleic acid accounts for up to 70%-80%of EVOO.Secondly,EVOO contains approximately more than 30 phenolic compounds,of which HT is essential for the protection against cardiovascular diseases.In this review,we focused on the potential mechanisms of oleic acid and polyphenols combat cardiovascular diseases risk in terms of oxidative stress,inflammation,blood pressure,endothelial function and cholesterol.This review might provide a reference for the studies on cardiovascular protective effects of EVOO. 展开更多
关键词 Cardiovascular diseases Extra virgin olive oil Oleic acid POLYPHENOLS
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Integrated Use of Organic and Bio-fertilizers to Improve Yield and Fruit Quality of Olives Grown in Low Fertility Sandy Soil in an Arid Environment 被引量:1
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作者 Bassam F.Alowaiesh M.M.Gad Mohamed Saleh M.Ali 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第6期1813-1829,共17页
Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers o... Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization. 展开更多
关键词 Organic and bio-fertilizers oliveS kalamata vegetative growth leaf mineral contents fruit quality
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Olive oil ameliorate allergic response in ovalbumin-induced food allergy mouse by promoting intestinal mucosal immunity
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作者 Yu Ma Ming Liu +5 位作者 Donghui Li Jie Li Zixin Guo Yunjun Liu Shengnan Wan Yixiang Liu 《Food Science and Human Wellness》 SCIE CSCD 2023年第3期801-808,共8页
The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by ... The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently constructed for analysis,the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group.In addition,analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial mucosal immunity. 展开更多
关键词 olive oil Ovalbumin-induced food allergy Anti-food allergy Intestinal mucosal immunization
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Production of Biogas from Olive Mill Waste Waters Treated by Cow Manure
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作者 Fatma Laabidi Rafik Besbès Bassem Jamoussi 《Green and Sustainable Chemistry》 CAS 2023年第1期62-71,共10页
The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. T... The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. The aim of this study was to valorize (OMWW) by anaerobic fermentation in the presence of cow manure in order to produce biogas and reduce their toxic load. Many tests were carried out by fermenting (OMWW) with polyphenols in the presence of cow manure in thermophile temperatures. The performance of this treatment was valuated through measurements of biogas production and by the determination of different parameters of fermented media (pH, volume of the biogas and polyphenols). 展开更多
关键词 olive Mill Waste Waters Anaerobic Fermentation Cow Manure BIOGAS POLYPHENOLS
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The Probable Cause for Nesting Pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar Beach, North East Coast of Andaman Island, India
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作者 Arun Malarvizhi M. M. Ilaamurughu P. M. Mohan 《Open Journal of Marine Science》 2023年第1期7-27,共21页
The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with th... The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with the nesting behaviour of the Olive Ridley turtles. In the present study, characteristics of the breeding beach and nesting pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of Andaman Islands were investigated, during the nesting periods 2016-2017. The study area hosts Olive Ridley, the dominant sea turtles with more than 300 individuals nesting each year. For this study, the number of sea turtles visited, nested, the sediment characters, salinity, and temperature were taken. The exposed sandy nesting beach characteristics are prone to varying degrees of morphological changes every day. The results depict that even though similar grain size (Coarse Sand to Fine Sand and Very well sorted to Poorly Sorted), with an ambient incubating temperature, pH and salinity with wide nesting area, the selective nesting in the particular location of the beach identified because of comfortable energy conditions in the waters (1.5 m/s) favours the female turtles to reach the beach at the preferable site of Ramnagar and nest. 展开更多
关键词 Sea turtle olive Ridley Ramnagar Beach Nesting Environment Morphological Changes Sand Grain HATCHLING North Andaman
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Nesting Biology and Site Selection of Olive Ridley—A Coherence of Nature
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作者 Arun Malarvizhi P. M. Mohan 《Open Journal of Marine Science》 2023年第2期29-39,共11页
Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochely... Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of North Andaman Islands were investigated, for the nesting periods 2016-2017. Olive Ridley is the dominant sea turtles, with more than 300 individuals nesting every season with at least a 75% hatching success rate. It is one of the conducive, highly protective and undisturbed nesting sites for Olive Ridley on North Andaman coast. In the present study an initiative is made, to identify nature’s cues and the biological characteristics of Olive Ridley, which leads it to nest at Ramnagar beach, with a high hatchling success rate. 展开更多
关键词 Sea Turtle olive Ridley Ramnagar Beach Nesting Beach Sand Grain Hatch-ling North Andaman
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Detection and Analysis of Squalene in Oil from Olive Fruits of Different Varieties and of Different Degrees of Maturity 被引量:2
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作者 耿树香 宁德鲁 +2 位作者 张艳丽 陈海云 李勇杰 《Agricultural Science & Technology》 CAS 2013年第10期1482-1485,共4页
[Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the o... [Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the objective to provide technical support for the harvest and processing of olive fruits. [Method] Taking 13 introduced olive varieties including three varieties of eight different grades of maturity, fat acid w3s first extracted by Soxhlet extraction and then squalene was quantitatively and qualitatively analyzed by gas chromatography-mass spectrometry (GC-MS) method. [Result] The results showed that squalene content was the highest in CG-32, and the lowest in Leccino among the 13 olive varieties. And squalene content increased with the degree of maturity in the three olive varieties including CG-32, Frantoio and Ascolana Tenera. [Conclusion] A more simple and rapid method for the determina- tion of squatene content was established. 展开更多
关键词 olive olive oil Determination of squalene content GC-MS method
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Protective Effects of Aqueous Extract of Eucommia ulmiodes Oliver Leaves on Liver Mitochondria 被引量:6
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作者 刘静 杨建雄 《Agricultural Science & Technology》 CAS 2013年第4期627-629,共3页
[Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves (AEO) on liver mitochondrial injury induced by free radicals. [Method] MDA content was determi... [Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves (AEO) on liver mitochondrial injury induced by free radicals. [Method] MDA content was determined by thiobarbituric acid colorimetric method; mitochondrial swelling degree was determined by spectrophotometry; the superoxide anion radical scavenging ability was determined using reduced coenzyme I-tetrazolium-phenazine methosulfate as the superoxide anion generation system. Mice were hypodermically injected in the back and neck with D- galactose, after 50 d, the effects of AEO on the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and anti-hydroxyl radicals in mouse liver were determined using kits. [Result] AEO can efficiently reduce the liver injury and inhibit mitochondrial swelling induced by Fe2+-L-Cys, which can also scavenge superoxide anion and improve the activities of antioxidant enzymes. [Conclusion] This study provided scientific basis for the development and application of AEO resources. 展开更多
关键词 Eucommia ulmiodes oliver leaves Antioxidase MITOCHONDRIA
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Introduction and Breeding of Olive Variety‘Picual' 被引量:1
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作者 李勇杰 宁德鲁 +3 位作者 贺娜 张艳丽 马婷 耿树香 《Agricultural Science & Technology》 CAS 2017年第12期2323-2325,共3页
The olive variety "Picual' is native to Jaen Province, Spain and is the main cultivar of olive. In 1979, scions were introduced into China and were grafted in Wuchang of Hubei, Chenggu of Shaanxi, Santai and Xichang... The olive variety "Picual' is native to Jaen Province, Spain and is the main cultivar of olive. In 1979, scions were introduced into China and were grafted in Wuchang of Hubei, Chenggu of Shaanxi, Santai and Xichang of Sichuan, and Kunming of Yunnan. Its fruit is oval, and the weight of a single fruit is 5.38 g. The top of fruit is rounded, and stigma exists. The base of fruit is flat. There is a mouth on the top of its fruit, and it is asymmetric. Oil content in fresh fruit is 24.69%, and oleic acid content is 77.5%; the oil quality is good. In the areas with cold winter in dry and hot valleys of the Jinsha River in Yunnan, its fruit matures in late September. In 2016, it was approved to be a good variety by Yunnan Forest Tree Variety Examination and Approving Committee. 展开更多
关键词 olive VARIETY Picual INTRODUCTION
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Prospects Expectation for Development of Oil Olive Industry Development in China from the Domestic and Foreign Olive Oil Market 被引量:2
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作者 张正武 王兆山 张建国 《Agricultural Science & Technology》 CAS 2017年第8期1541-1547,共7页
Over the past two years, China's olive Planting area was a bumper harvest year , extra virgin olive oil production has increased at rate of 30% an annual, the government, enterprises and farmers had should be present... Over the past two years, China's olive Planting area was a bumper harvest year , extra virgin olive oil production has increased at rate of 30% an annual, the government, enterprises and farmers had should be presented to the satisfaction of all the situation. However, a large number of farmer has listed to be bought of their fresh fruit , it was presented of 'queuing for salphenomenon, fruit can not be bought and pressed in time, farmer's enthusiasm was hurt and olive oil quality was reduced. In this regard, we had a comprehensive investigation and analysis of the current problems, combined with foreign research results, to researched the domestic and foreign olive oil market, has found our country disadvantages of in oil olive industry development and the existence question, and has carried on the forecast to our country oil olive industry development. Indicated that there is a lot of demand for olive oil consumer In China, and vigorously support at all levels of government, key factors restricting the healthy development of the industry had being resolved, speeding up the development of policies, technologies, talents,markets. The development prospect of olive industry is very broad. 展开更多
关键词 olive oil Market analysis EXPECTATION
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Evaluation of Drought Resistance of Olive Varieties from Different Provenances
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作者 李勇杰 张艳丽 +5 位作者 宁德鲁 贺娜 陈芳 耿树香 陈海云 毛云玲 《Agricultural Science & Technology》 CAS 2014年第11期1905-1907,共3页
In order to study olive’s drought resistance in Yunnan province, different varieties of olive trees from Albania, Italy, Spain and Greece were cultured, and leaves from these trees in drought season and rainy season ... In order to study olive’s drought resistance in Yunnan province, different varieties of olive trees from Albania, Italy, Spain and Greece were cultured, and leaves from these trees in drought season and rainy season were col ected and determined by physiological indices, such as proline content, relative electric con-ductivity, binding water/free water, relative water content and malon-dialdehyde in leaves. The evaluation of drought resistance with subordinate function showed that:There were no significant differences among drought resistances of varieties and provenances. The change of physiological indices in different varieties showed differ-ent ranges. The drought resistance of seven varieties decreased in order as fol ows:Koroneiki〉 Picual〉 Berat〉 Kaliniot〉 Frantoio〉 Coratina〉 Arbequina. 展开更多
关键词 Provenance olive Physiological indices Drought resistance
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Olive动态心电图分析系统临床使用报告
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作者 陈国伟 梁耀荣 《现代医学仪器与应用》 1991年第4期23-24,共2页
关键词 olive动态心电图 分析系统 监测
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Agronomic and Technological Factors Affecting Tunisian Olive Oil Quality
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作者 Ines Gharbi Manel Issaoui +3 位作者 Sounira Mehri Imed Cheraief Samira Sifi Mohammed Hammami 《Agricultural Sciences》 2015年第5期513-526,共14页
Olive oil is a very versatile product. It has distinctive virtues in the fields of health and nutrition. For this reason olive oil quality has attracted attention and become the focus of many studies. Olive oil qualit... Olive oil is a very versatile product. It has distinctive virtues in the fields of health and nutrition. For this reason olive oil quality has attracted attention and become the focus of many studies. Olive oil quality depends on several factors such as ripening, extraction method, soil type, climatic conditions, harvesting time, varieties and storage conditions. Quality assessment of olive oil is linked to an important series of physicochemical parameters including free fatty acid content, peroxide value and sensory evaluation. The main objective of this study is to investigate using statistical analysis, the main factors influencing the quality of Tunisian olive oils. Physicochemical analysis of 89 samples of olive oil produced in the region of the Sahel and central Tunisia. This study demonstrates that the main factors influencing Tunisian olive oil quality are: olive ripening, harvesting methods, olive pre-processing storage, olive washing, leaf removing, mixing, separation systems and crushing time. The data also shows that the commercial qualitative parameters of virgin olive oil such as free fatty acids, peroxide value, specific spectrophotometric absorptions in the UV region and sensorial assessment depend on the cultivar and quality of olives before processing. The application of good olive-growing practices complemented by studies similar to this would improve the quality of olive oil produced in Tunisia. This will contribute to the promotion and value of these oils as a regional product. 展开更多
关键词 olive Oil QUALITY Sensorial Assessment oliveS Pressure CENTRIFUGATION HARVEST
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Olive动态心电图分析系统伪差与误判的分析
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作者 陈虹心 《广东医学院学报》 1999年第2期156-157,共2页
24h动态心电图(ECG)作为一种无创性、可重复性的心脏电生理检查已广泛应用于临床,但在检查过程中,常出现伪差与误判,为探讨其原因,现将我院1996年5月至1998年3月间Olive动态ECG分析系统所检查的306例... 24h动态心电图(ECG)作为一种无创性、可重复性的心脏电生理检查已广泛应用于临床,但在检查过程中,常出现伪差与误判,为探讨其原因,现将我院1996年5月至1998年3月间Olive动态ECG分析系统所检查的306例分析如下:1临床资料本组男性166... 展开更多
关键词 动态心电图 伪差 误诊 olive
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Olive动态心电图监测系统使用情况报告
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作者 辛达临 《现代医学仪器与应用》 1991年第4期22-23,共2页
关键词 olive动态心电图 监测系统 检测 ST段
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思科助力Mercer Oliver Wyman部署IP通信
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作者 杨海峰 《通信世界》 2004年第44期51-51,共1页
思科系统的IP通信(IPC)应用的最新特性使网络企业可以更加轻松、更加经济地使用和部署融合的公司生产率解决方案。利用功能强大的统一通信工具——Clsco Unity的最新版本,跨国公司可以在部署统一语音和电子邮件通信的同时,降低成本、... 思科系统的IP通信(IPC)应用的最新特性使网络企业可以更加轻松、更加经济地使用和部署融合的公司生产率解决方案。利用功能强大的统一通信工具——Clsco Unity的最新版本,跨国公司可以在部署统一语音和电子邮件通信的同时,降低成本、提高生产率和改进客户服务。 展开更多
关键词 思科公司 IP通信 Mercer oliver Wyman公司 ClscoUnity
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Investigation and Analysis of the Current Situation of Olive-Stone Carving Inheritance in Guangzhou
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作者 Zhaofeng Hou 《Proceedings of Business and Economic Studies》 2021年第6期78-83,共6页
The issue of inheriting and developing traditional handicrafts in this contemporary society is being explored by all walks of life in China.Taking the olive-stone carving in Guangzhou as a case,field investigations an... The issue of inheriting and developing traditional handicrafts in this contemporary society is being explored by all walks of life in China.Taking the olive-stone carving in Guangzhou as a case,field investigations and analyses of its current situation of inheritance have been conducted.It has been found that although the government in Guangzhou has been paying more attention to olive-stone carving,there are still many problems,including the shortage of raw materials,no inheritors,low market recognition,and inadequate promotion.Therefore,the local government has cooperated with schools and craftsmen to search for key solutions in regard to raw material protection,training of inheritors,product development,and product promotion. 展开更多
关键词 olive-stone carving in Guangzhou olive stone CARVING HANDICRAFT INHERITANCE
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Olive oil consumption and non-alcoholic fatty liver disease 被引量:24
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作者 Nimer Assy Faris Nassar +1 位作者 Gattas Nasser Maria Grosovski 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第15期1809-1815,共7页
The clinical implications of non-alcoholic fatty liver diseases(NAFLD)derive from their potential to progress to fibrosis and cirrhosis.Inappropriate dietary fat intake,excessive intake of soft drinks,insulin resistan... The clinical implications of non-alcoholic fatty liver diseases(NAFLD)derive from their potential to progress to fibrosis and cirrhosis.Inappropriate dietary fat intake,excessive intake of soft drinks,insulin resistance and increased oxidative stress results in increased free fatty acid delivery to the liver and increased hepatic triglyceride(TG)accumulation.An olive oil-rich diet decreases accumulation of TGs in the liver,improves postprandial TGs,glucose and glucagonlike peptide-1 responses in insulin-resistant subjects, and upregulates glucose transporter-2 expression in the liver.The principal mechanisms include:decreased nuclear factor-kappaB activation,decreased lowdensity lipoprotein oxidation,and improved insulin resistance by reduced production of inflammatory cytokines(tumor necrosis factor,interleukin-6)and improvement of jun N-terminal kinase-mediated phosphorylation of insulin receptor substrate-1.The beneficial effect of the Mediterranean diet is derived from monounsaturated fatty acids,mainly from olive oil.In this review,we describe the dietary sources of the monounsaturated fatty acids,the composition of olive oil,dietary fats and their relationship to insulin resistance and postprandial lipid and glucose responses in non-alcoholic steatohepatitis,clinical and experimental studies that assess the relationship between olive oil and NAFLD,and the mechanism by which olive oil ameliorates fatty liver,and we discuss future perspectives. 展开更多
关键词 Liver steatosis Fatty liver olive oil Fatty acids Monounsaturated Non-alcoholic steatohepatitis LIPIDS Oleic acid Non-alcoholic fatty liver disease
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Olive leaf extract inhibits lead poisoning-induced brain injury 被引量:21
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作者 Yu Wang Shengqing Wang +3 位作者 Wenhui Cui Jiujun He Zhenfu Wang Xiaolu Yang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第22期2021-2029,共9页
Olive leaves have an antioxidant capacity, and olive leaf extract can protect the blood, spleen and hippocampus in lead-poisoned mice. However, little is known about the effects of olive leaf extract on lead-induced b... Olive leaves have an antioxidant capacity, and olive leaf extract can protect the blood, spleen and hippocampus in lead-poisoned mice. However, little is known about the effects of olive leaf extract on lead-induced brain injury. This study was designed to determine whether olive leaf extract can inhibit lead-induced brain injury, and whether this effect is associated with antioxidant capacity. First, we established a mouse model of lead poisoning by continuous intragastric administration of lead acetate for 30 days. Two hours after successful model establishment, lead-poisoned mice were given olive leaf extract at doses of 250, 500 or 1 000 mg/kg daily by intragastric administration for 50 days. Under the transmission electron microscope, olive leaf extract attenuated neuronal and capillary injury and reduced damage to organelles and the matrix around the capillaries in the frontal lobe of the cerebral cortex in the lead-poisoned mice. Olive leaf extract at a dose of 1 000 mg/kg had the greatest protective effect. Spectrophotometry showed that olive leaf extract significantly in- creased the activities of superoxide dismutase, catalase, alkaline phosphatase and acid phes- phatase, while it reduced malondialdehyde content, in a dose-dependent manner. Furthermore, immunohistochemical staining revealed that olive leaf extract dose-dependently decreased Bax protein expression in the cerebral cortex of lead-poisoned mice. Our findings indicate that olive leaf extract can inhibit lead-induced brain injury by increasing antioxidant capacity and reducing apop- tosis. 展开更多
关键词 neural regeneration traditional Chinese medicine olive leaf extract lead brain injury SUPEROXIDEDISMUTASE CATALASE alkaline phosphatase acid phosphatase MALONDIALDEHYDE apoptosis neu-ropathology grants-supported paper NEUROREGENERATION
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