A study on the activation of 8 oncogenes and inactivation of 2 oncosuppressor genes in human gastric cancer

The changes of 8 oncogenes and 2 oncosuppressor genes in the specimens of cancer-ous and juxtacancerous tissues of 42 cases of gastric cancer were studied with southern blot hy-bridization and PCR-RFLP method.The probes used were c-Ha-ras,K-ras,N-ras,N-myc,c-myc,hst,EGFR,c-erbB-2,p53 and Rb.Amplification,rearrangement and deletion of c-Ha-ras were detected in 8/33 (24.2%) cases of gastric cancer,amplification or rearrangement ofhst and c-erbB-2 in 11/42 (26.2%) and 12/41 (29.2%) cases respectively,amplification ofEGFR in 9/42 (21.4%) cases,and deletion or rearrangement of p53 and Rb in 9/30 (30.0%)and 2/15 (13.3%) cases respectively.Amplification or rearrangement of N-ras (0/33),K-ras(1/26),N-myc (1/26),and c-myc (1/35)was rarely encountered.These findings suggest thatc-Ha-ras,hst,c-erbB-2,EGFR and p53 may be important genes involved in the pathogenesisof gastric cancer.

MMR蛋白、p53、HER-2与Ki-67在子宫内膜癌组织中表达及与临床病理特征的关系

目的探讨错配修复(MMR)蛋白、抑癌基因p53(p53)、表皮生长因子受体2(HER-2)与肿瘤增殖抗原(Ki-67)在子宫内膜癌组织中表达及与临床病理特征的关系。方法选取濮阳市人民医院2019年1月至2021年1月收治的100例(均完成随访)子宫内膜癌患者为研究对象,采用免疫组化法检测子宫内膜癌组织和癌旁组织中MMR蛋白(MLH1、PMS2、MSH2、MSH6)、p53、HER-2、Ki-67表达情况,比较不同病理学参数子宫内膜癌组织中MMR、p53、HER-2、Ki-67的表达情况,分析子宫内膜癌组织中MMR、p53、HER-2、Ki-67表达与临床病理学参数的关系,分析MMR、p53、HER-2、Ki-67表达与子宫内膜癌患者预后关系。结果子宫内膜癌组织中MMR表达缺失率、p53、HER-2、Ki-67阳性表达率高于癌旁组织(P<0.05);不同病理类型患者子宫内膜癌组织MMR表达缺失率、p53、HER-2、Ki-67阳性表达率比较:Ⅰ~Ⅱ期低于Ⅲ~Ⅳ期,高分化<中分化<低分化,无淋巴结转移低于有淋巴结转移(P<0.05);子宫内膜癌组织MMR表达缺失率、p53、HER-2、Ki-67阳性表达率与临床分期、淋巴结转移均呈正相关,与组织分化程度呈负相关(P<0.05);MMR表达缺失、p53、HER-2、Ki-67阳性子宫内膜癌患者2年生存率分别低于MMR表达完整、p53、HER-2、Ki-67阴性子宫内膜癌患者(P<0.05)。结论MMR蛋白、p53、HER-2、Ki-67表达情况与子宫内膜癌发生发展显著相关,且在预测预后方面具有积极意义。