Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between pr...Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)storage.Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed significantly.Total sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation.The Fresh(FS),MAP,and air packaging(AP)groups were set.Total of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS group.The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover.Correlation analysis found that 52 DAPs were correlated with quality traits.Among them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity.These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.展开更多
Effects of copper toxicity and salinity shock on selective group of juvenile pompano Trachinotus ovatus were investigated.The fish were exposed to different Cu2+ concentrations of 0(blank),0.02(C1),0.05(C2),0.10(C3),a...Effects of copper toxicity and salinity shock on selective group of juvenile pompano Trachinotus ovatus were investigated.The fish were exposed to different Cu2+ concentrations of 0(blank),0.02(C1),0.05(C2),0.10(C3),and 0.15 mg·L–1(C4)at a salinity of 10‰ or 40‰ for 96 h,with the salinity of 29‰ as the control.The results showed that the effects of the acute salinity stimulation to survival rates of pompano between control(29‰)and lower or higher salinity for 96 h were not significant(p>0.05).However,the survival rates in each treatment were decreased with the increase of Cu2+ concentration.The dominant factor influencing body moisture of the fish was salinity,and there was no sign that body moisture was correlated with exposure to Cu2+.The gill lamellas in high level of Cu2+ concentration(C4 treatments)were abnormal under the salinity of 40‰ and extremely curly under the salinity of 10‰.Hemorrhage in gill was observed in the two C4 treatments.Under transmission electron microscope,pillar cells in gill lamellas appeared deformed and ruptured in some areas of the epithelia in the higher concentration of Cu2+,resulting in the death of the fish due to the destruction of gill tissue,elevation of the arithmetic mean distance from water to blood,the decrease of oxygen diffusion capacity,and other physiological functions.These findings indicate that the pompano might suffer much more pressure when encountered with Cu2+ pollution and low salinity.展开更多
A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor ceme...A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor cement pools and net pens showed that, in the first 15 days and 30 days, the growth of 1 day and 2 days-deprived groups was probably the same with that of control group, which accorded with fully compensatory description. However, the weight of 5 days and 7 days-deprived fingerlings were lower than that of the control group, which accorded with the description of the partial compensatory growth. As the starvation prolonged, feed conversion efficiency of the starved groups increased and was higher than the control group. Meanwhile, feeding rate also increased, but the fish of 1 day and 2 days-deprived groups was lower than that of the control group, and feeding quantity reduced and was lower than that of the control group. It suggested that the mechanism of compensatory growth was mainly due to improving feed conversion efficiency. Fish biochemical composition was analyzed： the water content and ash of fish sample increased due to starvation, while the lipid and protein decreased. The loss of lipid was greater than that of the protein, and the biochemical composition of fish in each group was restored to the control level by the end of the experiment. It suggested that Trachinotus ovatus may mainly consume lipid during the period of starvation.展开更多
The research aimed to create a shelf life prediction model for Trachinotus ovatus in different freezing temperatures by using back propagation(BP)neural network model.The pH,total volatile basic nitrogen(TVB-N),thioba...The research aimed to create a shelf life prediction model for Trachinotus ovatus in different freezing temperatures by using back propagation(BP)neural network model.The pH,total volatile basic nitrogen(TVB-N),thiobarbituric acid(TBA),water retention(water holding capacity[WHC];cooking loss),and sensory evaluation were measured under 266 K,255 K,243 K,233 K,and 218 K temperatures.The results of TVB-N and water retention during 266 K,255 K,233 K,and 218 K conditions were selected to build a BP neural network model and verify the model at 243 K.Results indicated that low temperatures retarded the rise of pH,TVB-N,and TBA values,improving water retention capacity of Trachinotus ovatus.The BP neural network model had high regression coefficients(r2:0.8642-0.9904),low mean square error(MES:0.1658-1.7882),and relative error within 10%and could accurately predict the quality change of Trachinotus ovatus under the freezing temperatures of 266 K-218 K.Therefore,(BP)neural network model has great potential in predicting the shelf life of Trachinotus ovatus in frozen storage.展开更多
This study aimed to isolate and identify the novel potential Xanthine oxidase(XOD)inhibitory peptides derived from Trachinotus ovatus hydrolysate(TOH)(hydrolysis by neutral protease),and investigate their structure-fu...This study aimed to isolate and identify the novel potential Xanthine oxidase(XOD)inhibitory peptides derived from Trachinotus ovatus hydrolysate(TOH)(hydrolysis by neutral protease),and investigate their structure-function relationship.Initially,TOH was separated to four fractions(F1–F4)by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate(85.16%±0.566,25 mg/mL)and lower molecular weight(95.73%,<1000 Da).F4 was further characterized by reversed-phase high performance liquid chromatography(RP-HPLC)coupled with Nano-HPLC-MS/MS and four peptides(FPAW,LLPW,WLLP and FHLP)were identified with IC50 value of 3.81±0.18 mM,4.17±0.12 mM,43.06±0.73 mM,and over 50.00 mM respectively.It can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD effectively.Moreover,the XOD-inhibiting actions of these peptides involvedπ-πstacking(Phe914 and Phe1009 in XOD),hydrogen bonding via molecular docking.Lineweaver-Burk equation showed the inhibition type of FPAW was a mixed inhibition.Circular dichroism(CD)spectra indicated FPAW could combine and change the secondary structure of XOD.Therefore,two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.展开更多
Type 2 diabetes(T2D)is highly associated with obesity.However,the factors that drive the transition from excessive weight gain to glucose metabolism disruption are still uncertain and seem to revolve around systemic i...Type 2 diabetes(T2D)is highly associated with obesity.However,the factors that drive the transition from excessive weight gain to glucose metabolism disruption are still uncertain and seem to revolve around systemic immune disorder.Mucosal-associated invariant T(MAIT)cells,which are innate-like T cells that recognize bacterial metabolites,have been reported to be altered in obese people and to lead to metabolic dysfunction during obesity.By studying the immunophenotypes of blood MAIT cells from a cross-sectional cohort of obese participants with/without T2D,we found an elevation in CD27^(-)negative(CD27−)MAIT cells producing a high level of IL-17 under T2D obese conditions,which could be positively correlated with impaired glucose metabolism in obese people.We further explored microbial translocation caused by gut barrier dysfunction in obese people as a triggering factor of MAIT cell abnormalities.Specifically,accumulation of the bacterial strain Bacteroides ovatus in the peripheral blood drove IL-17^(-)producing CD27−MAIT cell expansion and could be associated with T2D risk in obese individuals.Overall,these results suggest that an aberrant gut microbiota–immune axis in obese people may drive or exacerbate T2D.Importantly,CD27−MAIT cell subsets and Bacteroides ovatus could represent targets for novel interventional strategies.Our findings extend current knowledge regarding the clinical relevance of body mass index(BMI)-associated variation in circulating MAIT cells to reveal the role of these cells in obesity-related T2D progression and the underlying cellular mechanisms.展开更多
The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of t...The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of this fish.To examine sex determination-and gonadal development-related genes,we conducted transcriptome analysis of the ovaries and testes of T.ovatus.A total of 345,972,132 high-quality clean reads were obtained from 12 libraries.In addition,28,137 gonad-expressed unigenes were obtained by mapping the clean reads to the T.ovatus reference genome.A total of 8,237 differentially expressed genes(DEGs)were identified between stage I ovaries and testes,including 3,235 testicular upregulated and 5,002 ovarian upregulated genes.Furthermore,13,448 DEGs were obtained between stage III ovarian and testicular libraries,including 7,576 testicular upregulated and 5,872 ovarian upregulated genes.The DEGs included some sex-determining genes such as sry,dmrt1,and amh.DEGs between ovarian and testicular libraries were significantly enriched with KEGG pathways that are involved in gonadal development,sex determination,and gonadal function.Then,10 DEGs(seven testicular upregulated and three ovarian upregulated unigenes)were selected for quantitative real-time PCR analysis.Four genes(zinc-binding protein A33-like,pro-opiomelanocortin-like,leucine-rich repeat and transmembrane domain-containing protein 2-like,and forkhead boxC1)showed a specific testicular expression pattern.The gonadally expressed as well as testicular and ovarian DEGs provide useful information for further research on the reproductive biology of T.ovatus.展开更多
基金supported by Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD74,2023TD78)the Earmarked Fund for CARS-47(CARS-47)+2 种基金Guangdong Provincial Science and Technology Plan Project(2023B0202010015)Central Public-Interest Scientific Institution Basal Research Fund,CAFS(Sanya Yazhou Bay Science and Technology City(SKJC-2020-02-013))Special Funds for Promoting Economic Development in Guangdong Province(For Modern Fishery)(YueNong 2019B14).
文摘Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)storage.Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed significantly.Total sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation.The Fresh(FS),MAP,and air packaging(AP)groups were set.Total of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS group.The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover.Correlation analysis found that 52 DAPs were correlated with quality traits.Among them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity.These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.
基金supported by Special Scientific Research Funds for Central Non-profit Institutes,Chinese Academy of Fishery Sciences(2012A0401,2013A0501)
文摘Effects of copper toxicity and salinity shock on selective group of juvenile pompano Trachinotus ovatus were investigated.The fish were exposed to different Cu2+ concentrations of 0(blank),0.02(C1),0.05(C2),0.10(C3),and 0.15 mg·L–1(C4)at a salinity of 10‰ or 40‰ for 96 h,with the salinity of 29‰ as the control.The results showed that the effects of the acute salinity stimulation to survival rates of pompano between control(29‰)and lower or higher salinity for 96 h were not significant(p>0.05).However,the survival rates in each treatment were decreased with the increase of Cu2+ concentration.The dominant factor influencing body moisture of the fish was salinity,and there was no sign that body moisture was correlated with exposure to Cu2+.The gill lamellas in high level of Cu2+ concentration(C4 treatments)were abnormal under the salinity of 40‰ and extremely curly under the salinity of 10‰.Hemorrhage in gill was observed in the two C4 treatments.Under transmission electron microscope,pillar cells in gill lamellas appeared deformed and ruptured in some areas of the epithelia in the higher concentration of Cu2+,resulting in the death of the fish due to the destruction of gill tissue,elevation of the arithmetic mean distance from water to blood,the decrease of oxygen diffusion capacity,and other physiological functions.These findings indicate that the pompano might suffer much more pressure when encountered with Cu2+ pollution and low salinity.
基金Supported by Ocean Public Welfare Scientific Research Project,State Oceanic Administration of the People's Republic of China(Grant No.201205028)Key Projects in the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(2011BD13B11)Hainan Scientific Operating Expense Projects and Major Project of Science and Technology(ZDZX2013009,ZDZX2013014)
文摘A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor cement pools and net pens showed that, in the first 15 days and 30 days, the growth of 1 day and 2 days-deprived groups was probably the same with that of control group, which accorded with fully compensatory description. However, the weight of 5 days and 7 days-deprived fingerlings were lower than that of the control group, which accorded with the description of the partial compensatory growth. As the starvation prolonged, feed conversion efficiency of the starved groups increased and was higher than the control group. Meanwhile, feeding rate also increased, but the fish of 1 day and 2 days-deprived groups was lower than that of the control group, and feeding quantity reduced and was lower than that of the control group. It suggested that the mechanism of compensatory growth was mainly due to improving feed conversion efficiency. Fish biochemical composition was analyzed： the water content and ash of fish sample increased due to starvation, while the lipid and protein decreased. The loss of lipid was greater than that of the protein, and the biochemical composition of fish in each group was restored to the control level by the end of the experiment. It suggested that Trachinotus ovatus may mainly consume lipid during the period of starvation.
基金supported by China Agricultural Research System(CARS-47-G26)National Key R&D Program of China(2019YFD0901602)Ability promotion project of Shanghai Municipal Science and Technology Commission Engineering Center(19DZ2284000).
文摘The research aimed to create a shelf life prediction model for Trachinotus ovatus in different freezing temperatures by using back propagation(BP)neural network model.The pH,total volatile basic nitrogen(TVB-N),thiobarbituric acid(TBA),water retention(water holding capacity[WHC];cooking loss),and sensory evaluation were measured under 266 K,255 K,243 K,233 K,and 218 K temperatures.The results of TVB-N and water retention during 266 K,255 K,233 K,and 218 K conditions were selected to build a BP neural network model and verify the model at 243 K.Results indicated that low temperatures retarded the rise of pH,TVB-N,and TBA values,improving water retention capacity of Trachinotus ovatus.The BP neural network model had high regression coefficients(r2:0.8642-0.9904),low mean square error(MES:0.1658-1.7882),and relative error within 10%and could accurately predict the quality change of Trachinotus ovatus under the freezing temperatures of 266 K-218 K.Therefore,(BP)neural network model has great potential in predicting the shelf life of Trachinotus ovatus in frozen storage.
基金supported by the Key-Area Research and Development Program of Guangdong Province(2020B1111030004)the Guangdong Basic and Applied Basic Research Foundation(2019A1515011588)+4 种基金the Pearl River S&T Nova Program of Guangzhou(201906010081)the China Agriculture Research System of MOF and MARA(CARS-47)the Special Scientific Research Funds for Central Non-profit Institutes,Chinese Academy of Fishery Sciences(2020TD69)the Central Public-interest Scientific Institution Basal Research Funds,South China Sea Fisheries Research Institute,CAFS(2021SD06)Qingdao Science and technology benefit people special project(20-3-4-31-nsh).
文摘This study aimed to isolate and identify the novel potential Xanthine oxidase(XOD)inhibitory peptides derived from Trachinotus ovatus hydrolysate(TOH)(hydrolysis by neutral protease),and investigate their structure-function relationship.Initially,TOH was separated to four fractions(F1–F4)by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate(85.16%±0.566,25 mg/mL)and lower molecular weight(95.73%,<1000 Da).F4 was further characterized by reversed-phase high performance liquid chromatography(RP-HPLC)coupled with Nano-HPLC-MS/MS and four peptides(FPAW,LLPW,WLLP and FHLP)were identified with IC50 value of 3.81±0.18 mM,4.17±0.12 mM,43.06±0.73 mM,and over 50.00 mM respectively.It can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD effectively.Moreover,the XOD-inhibiting actions of these peptides involvedπ-πstacking(Phe914 and Phe1009 in XOD),hydrogen bonding via molecular docking.Lineweaver-Burk equation showed the inhibition type of FPAW was a mixed inhibition.Circular dichroism(CD)spectra indicated FPAW could combine and change the secondary structure of XOD.Therefore,two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.
基金This study was funded by the National Key R&D Program of China(2017YFA0105803)the National Natural Science Foundation of China(32000621 and 81770826)+3 种基金the Key Area R&D Program of Guangdong Province(2019B020227003)the Science and Technology Plan Project of Guangzhou City(202102010338 and 202007040003)the 5010 Clinical Research Projects of Sun Yat-sen University(2015015)the Dengfeng Plan High-level Hospital Construction Opening Project of Foshan Fourth People’s Hospital(FSSYKF-2020009).
文摘Type 2 diabetes(T2D)is highly associated with obesity.However,the factors that drive the transition from excessive weight gain to glucose metabolism disruption are still uncertain and seem to revolve around systemic immune disorder.Mucosal-associated invariant T(MAIT)cells,which are innate-like T cells that recognize bacterial metabolites,have been reported to be altered in obese people and to lead to metabolic dysfunction during obesity.By studying the immunophenotypes of blood MAIT cells from a cross-sectional cohort of obese participants with/without T2D,we found an elevation in CD27^(-)negative(CD27−)MAIT cells producing a high level of IL-17 under T2D obese conditions,which could be positively correlated with impaired glucose metabolism in obese people.We further explored microbial translocation caused by gut barrier dysfunction in obese people as a triggering factor of MAIT cell abnormalities.Specifically,accumulation of the bacterial strain Bacteroides ovatus in the peripheral blood drove IL-17^(-)producing CD27−MAIT cell expansion and could be associated with T2D risk in obese individuals.Overall,these results suggest that an aberrant gut microbiota–immune axis in obese people may drive or exacerbate T2D.Importantly,CD27−MAIT cell subsets and Bacteroides ovatus could represent targets for novel interventional strategies.Our findings extend current knowledge regarding the clinical relevance of body mass index(BMI)-associated variation in circulating MAIT cells to reveal the role of these cells in obesity-related T2D progression and the underlying cellular mechanisms.
基金the National Natural Science Foundation of China(Grant No.31660740)the Fundamental Research Funds for nonprofit research institutes under Guangxi Zhuang Autonomous Region(Grant No.GXIF-2016-19)+1 种基金Science and Technology Major Project of Guangxi[Grant number AA18242031]Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture Opening Fund(Grant No.16-380-45-B-3).
文摘The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of this fish.To examine sex determination-and gonadal development-related genes,we conducted transcriptome analysis of the ovaries and testes of T.ovatus.A total of 345,972,132 high-quality clean reads were obtained from 12 libraries.In addition,28,137 gonad-expressed unigenes were obtained by mapping the clean reads to the T.ovatus reference genome.A total of 8,237 differentially expressed genes(DEGs)were identified between stage I ovaries and testes,including 3,235 testicular upregulated and 5,002 ovarian upregulated genes.Furthermore,13,448 DEGs were obtained between stage III ovarian and testicular libraries,including 7,576 testicular upregulated and 5,872 ovarian upregulated genes.The DEGs included some sex-determining genes such as sry,dmrt1,and amh.DEGs between ovarian and testicular libraries were significantly enriched with KEGG pathways that are involved in gonadal development,sex determination,and gonadal function.Then,10 DEGs(seven testicular upregulated and three ovarian upregulated unigenes)were selected for quantitative real-time PCR analysis.Four genes(zinc-binding protein A33-like,pro-opiomelanocortin-like,leucine-rich repeat and transmembrane domain-containing protein 2-like,and forkhead boxC1)showed a specific testicular expression pattern.The gonadally expressed as well as testicular and ovarian DEGs provide useful information for further research on the reproductive biology of T.ovatus.
