[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sod...[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.展开更多
Pot experiments were conducted in a glasshouse to determine the growth pattern of lettuce, true potato seedlings (TPS) and single node cuttings of TPS in response to biochar. The treatments were arranged in a random...Pot experiments were conducted in a glasshouse to determine the growth pattern of lettuce, true potato seedlings (TPS) and single node cuttings of TPS in response to biochar. The treatments were arranged in a randomized complete block design with 5 treatments (0, 10, 30, 50 and 100 t ha^-1) ofbiochar from greenwaste with 5 replications in lettuce, 10 in TPS and 5 in single node cuttings of TPS. The observations recorded on growth parameters showed that biochar had significant effect on growth of lettuce but no consistent effect on growth of TPS and single node cuttings. Among the biochar rates, 30 t ha^-1 had the greatest influence on overall growth of lettuce. The pH and electrical conductivity increased as the biochar rates increased in all experiments. These results provide an avenue for soil management system by using biochar as an amendment in horticultural crops. However, their verification in the field is important for specific recommendations.展开更多
With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results s...With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results showed that under the stress of Pb (200 mg/kg) and Cd (20 mg/kg), growth of potato had no obvious difference with that in control group; under the stress of Pb ( 〉200 mg/kg) and Cd ( 〉20 mg/kg), potato growth suspended, and its physiological indexes such as dry weight, plant height, root number, root length were inhibited obviously. With the increasing mass fraction of Pb, relative electrical conductivity, MDA and proline of potato leaves showed the tendency of increase, and the results in high mass fraction treatments showed significant difference with those in the control group (P 〈 0.05 ), CAT, POD and SOD activity of leaves increased greatly with the increasing mass fraction. In the Pb stress treatment (200 mg/kg), relative electrical conductivity, MDA and proline of potato leaves showed no obvious difference with those in the control group, and the results in high mass fraction treatments were significantly higher than those in the control group ( P 〈 O. 05 ), CAT and SOD activity increased greatly with the increasing mass fraction ( P 〈 0.05 ). Under the stress of Pb and Cd, synthesis of soluble protein in potato leaves was strongly inhibited. The results showed that potato was tolerant to Pb and Cd of lower mass fraction, and damage of potato caused by Pb and Cd stress could be relieved by improving activity of antioxidase and reducing peroxidation of membrane lipid, and the tolerance reduced with the increase of stress.展开更多
With the detoxicated seedling of a potato cultivation breed named “Mire” as the material, the effect of auxins CCC, 6-BA, and active carbon to microtubers of potato (Solanum tubersum L.) was investigated under the i...With the detoxicated seedling of a potato cultivation breed named “Mire” as the material, the effect of auxins CCC, 6-BA, and active carbon to microtubers of potato (Solanum tubersum L.) was investigated under the in-vitro circumstances. The result indicated the exogenous auxins improved the production and quality of microtubers of potatoes. The effect of induction can be described as CCC > CCC + 6-BA > 6-BA > CK, the number of microtubers in per flask is 8.17 > 7.67 > 7.29 > 5.46, and the number of large potatoes in per flask is 6.33 > 5.17 > 3.17 > 1. In addition, by adding 0.5‰ of active charcoal, the growth period was shortened from 25.0 days to 9.33 days on average, and the amount of larger potatoes increased 8.54%. These results benefited the growth of microtubers of potato.展开更多
Two separate experiments were conducted to evaluate the success of the establishment and growth of micro-cuttings of potato (5 - 6 cm tall) in sand trays [38 cm (L) × 28 cm (W) × 7.5 cm (H) plastic trays] un...Two separate experiments were conducted to evaluate the success of the establishment and growth of micro-cuttings of potato (5 - 6 cm tall) in sand trays [38 cm (L) × 28 cm (W) × 7.5 cm (H) plastic trays] under controlled environment (22?C ± 2?C, 60 - 75 μmosm–1?s–1 light energy for 16 h daily). In the first experiment, micro-cuttings of potato cv. Diamant were planted at six populations (500, 600, 700, 800, 900 and 1000 cuttings per tray) in treated sand (sun dry, 1% formaldehyde, 0.2% Dithane M-45 and control). The mortality percentage of micro-cuttings was nil for sun dry sand while formaldehyde and dithane M-45 treated sand had 1% - 4% against 15% in the control with the highest population density. Mortality of micro-cuttings in formaldehyde and dithane M-45 treated sand trays were found not to be related to pathogenic organism rather toxic effect of these two chemicals. Micro-cuttings in Sun dry and control treatments showed better growth performance than these in chemically treated sand trays. In the second experiment, urea @ 1, 2 and 3 g per tray was applied as solid form after 15 days of planting the micro-cuttings and as liquid form @ 0.5, 1 and 2% solution sprayed in the micro-cuttings repeatedly after 15, 30, 45 and 60 days of planting. The micro-cuttings which received urea as solid state died within 2 - 3 days and 2% urea solution was also detrimental. Urea solution @ 0.5% found to be very effective for vegetative growth of micro-cuttings in sand trays. The control was also good for vegetative growth but at a slower rate.展开更多
The study was carried out to determine the influence of sweet potato peels and cashew nut shell supplemented with Ocimum gratissium leaves, using growth indices and blood constituents by goats. Eighteen goats with an ...The study was carried out to determine the influence of sweet potato peels and cashew nut shell supplemented with Ocimum gratissium leaves, using growth indices and blood constituents by goats. Eighteen goats with an average weight of 8.11 kg and aged between 8 and 9 months old were allotted to three dietary treatments with six animals per treatment in a complete randomized design. The compared diets which comprised combination of sweet potato peels and cashew nut shell with concentrate respectively were in different ratios which included diet I (30:25:45), diet II (25:30:45) and diet III (20:35:45). Each goat also received 8 grams of Ocimum gratissium leaves as supplement in the diet. The results showed that average feed intake (6.42 kg) and fed conversion ratio (2.15) were highest in diet I and significantly different from other diets. Diet II had the highest significant (P 3/L), lymphocytes (56.01%), monocytes (1.06%), total protein (7.65 g/dl), albumin (3.58 g/dl), globulin (4.07 g/dl) and triglyceride (2.29 mmol/L). White blood cell (7.01 × 103/L), neutrophils (49.82%), cholesterol (42.34 mmol/L), creatnine (1.59 mg/dl) and urea (18.29 mg/dl) were (P < 0.05) best in diet III, whereas initial bodyweight, average daily weight gain, eosinophils and basophils were not significantly different (P < 0.05). It is concluded that diet II has the potential to enhance growth rate and blood constituents of goats.展开更多
Potato grows in most part of China, it achieves higher yield and better quality in Gansu Province than in others. With global warming, its growth duration has been prolonged and sowing date become earlier than before....Potato grows in most part of China, it achieves higher yield and better quality in Gansu Province than in others. With global warming, its growth duration has been prolonged and sowing date become earlier than before. Therefore, to regulate its sowing date and growing period is of great significance for better harvest. In this study, experiments were conducted with six sowing-date treatments of potato in Dingxi, which is in the Loess Plateau of central Gansu Province in Northwest China in 2010. The growth period, morphological index and change in yield and their relationships with temperature, precipitation, and other climatic factors were investigated for each treatment. Results show that the crop with different sowing dates experienced different climate conditions, leading to distinct growth duration, plant height, and leaf area index. The growth duration was shortened due to a delay in sowing date. For each 15-day delay in sowing, the growth duration was reduced by 12 days on average. A significant linear relationship was found between numbers of days either from seeding to emergence or from flowering to harvest and mean temperature over the corresponding period. Dry matter accumulation, tuber fresh weight, and final yield were all decreased because of insufficient cumulative temperature over the shorter growing periods. Marked differences in tuber yield were discovered among the six treatments of sowing date, the potato planted on May 27 giving the highest yield. The potato planted either earlier or later would produce invariably lower yield than the treatment of May 27. Late May therefore can be taken as the optimum sowing time of potato in this region because the crop can fully utilize thermal resource. We conclude that to postpone sowing time is a good practice for potato production to adapt to climate warming in the Loess Plateau of central Gansu, China.展开更多
Different aspects of micropropagation through meristem culture for the production of virus indexed source plants, <i><span style="font-family:Verdana;">in vitro</span></i><span sty...Different aspects of micropropagation through meristem culture for the production of virus indexed source plants, <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> tuberization and field evaluation of the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> regenerated plants were studied on four commercial cultivars of potato (</span><i><span style="font-family:Verdana;">Solanum tuberosum</span></i><span style="font-family:Verdana;"> L.) viz., Diamant, Cardinal, Shilbilati and Lalpakri. The investigation was conducted at Rajshahi, Bangladesh from December 2010 to March 2012 to produce virus-free potato plantlets through meristem culture, shoot multiplications with root induction as well as their acclimatization and evaluation of morphological characters and tuber yield under field condition. Shoot tips of 25</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">30 day old field</span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">grown plants of above mentioned four cultivars were used for meristem isolation. After isolation, meristems of these varieties of potato were cultured on “M” shaped filter paper bridge in Murashige and Skoog (MS) liquid medium. Four different treatments of media formulations viz. 0.1 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, 0.1</span></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, 0.5 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> and 0.5 mg/L KI</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">N + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> were used as plant growth regulators. From these formulations MS + 0.1 mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> was found to be the best for </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">primary establishment of meristem culture. The primar</span><span style="font-family:Verdana;">ily</span><span style="font-family:Verdana;"> established meristems were subcultured on to MS semisolid basal medium supplemented with four different treatment combinations of hormones viz. 0.5 mg/L BA</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">+ 1.0 mg/L IBA;0.1 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">;0.5 mg/L BA + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> and 0.5 mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> were used to identify the suitable media compositions for shoot proliferation. Results showed that out of these four media treatments the formulation 0.5 mg/L BA + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> was found to be the best suitable for shoot generation. Among the four cultivars of potato higher frequency of shoot proliferation (number of shoots/explant and longest shoot length) was observed in Diamant, though the highest shoot formation (76%) was recorded in Cardinal. Virus free </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> grown potato plantlets were ob</span><span style="font-family:Verdana;">tained through DAS-ELISA test and used substantially for m</span><span style="font-family:Verdana;">icro-propagation. After gradual acclimatization of rooted plantlets of four potato cultivars</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> they were transferred into the field for cultivation and established successfully. It was observed from the field study of </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> meristem-derived plantlets that there were no virus-affected plants. The virus-free exotic varieties were much superior in all vegetative attributes and yield compare</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> to those of indigenous varieties with producing potato plants of normal height. In contrast, the indigenous varieties took </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">longer time to tuber initiation and maturity, lower plant height and number of leaves per plant, </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">higher number of tubers but </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">lower amount of tuber weight per plant, and poorer tuber grade than the exotic varieties. However, the variety Cardinal exposed the best performances in the context of survival percentage of plantlets (90%), days to tuber initiation (DTI), </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">number of leaves per plant (NL), tuber weight per plant (343.40%) and the percentage of rich tuber grade.</span>展开更多
This study consisted to evaluate the effects of ecological practices on the yield performance of the JEWEL of orange-fleshed sweet potato (Ipomoea batatas (L.) Lam). The study was conducted in a Sudano-Sahelian croppi...This study consisted to evaluate the effects of ecological practices on the yield performance of the JEWEL of orange-fleshed sweet potato (Ipomoea batatas (L.) Lam). The study was conducted in a Sudano-Sahelian cropping system (Lantargou, eastern region of Burkina Faso). Agro-ecological practices consisted of the inputs of 3.20 t/ha of compost + 2.45 t/ha of wood ash (CO + WA);4.90 t/ha of wood ash (WA);6.40 t/ha of compost (CO) were compared to control with no inputs (T<sub>0</sub>). Each treatment was repeated four times. The crop management consisted of plowing, harrowing, raising of ridges with 40 cm height, burying of treatments, transplanting of cuttings, two weeding’s, and using of biopesticide called PIOL for crop protection. Composite soil samples per treatment were also collected at tuber harvest and analyzed to determine the effects of treatments on residual soil fertility. Results showed that the plant heights and diameters under the CO were significantly (P < 0.001) improved by 16% and 12% compared to T<sub>0</sub>. The WA treatment significantly increased the number of large tubers by 43% (P ≤ 0.01) compared to T<sub>0</sub>. Total tuber numbers, large tuber numbers and tuber yields of sweet potato under CO + WA were significantly (P < 0.001) and respectively improved by 27%, 50% and 31% compared to T<sub>0</sub>. All treatments increased soil organic matter, N, P and K contents, and reduced soil acidity compared with those obtained under T<sub>0</sub>. Soil K content was improved by 39% under CO + WA, and soil N content by 34% under WA compared to T<sub>0</sub>. Soil C/N ratio under CO + WA was reduced by 20% compared others treatments. But, the CO + WA treatment outperformed by improving residual soil N content by 38%, and the WA treatment by increasing soil K content by 50% compared to T<sub>0</sub>. In addition, soil pHH<sub>2</sub>O increased by 1.2 units under WA treatment compared to T<sub>0</sub>. As conclusion, the application of 6.4 t/ha of compost performed well to improve the vegetative growth of orange-fleshed sweet potato while the inputs of 3.2 t/ha of compost + 2.45 t/ha of wood ash were efficacy to significantly increase the tuber yields and improve the residual fertility of soil.展开更多
基金Supported by the National Natural Science Foundation(30670177)Scientific Research Foundation for the Outstanding Young Scientist of Shangdong Province(006BS06002)National Ministry of Education Doctoral Fund(20050445003)~~
文摘[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.
基金financially supported by Horticulture Australia Ltd.,using voluntary contributions from Brisbane City Council and matched funds from the Australian Government (code HG10025)
文摘Pot experiments were conducted in a glasshouse to determine the growth pattern of lettuce, true potato seedlings (TPS) and single node cuttings of TPS in response to biochar. The treatments were arranged in a randomized complete block design with 5 treatments (0, 10, 30, 50 and 100 t ha^-1) ofbiochar from greenwaste with 5 replications in lettuce, 10 in TPS and 5 in single node cuttings of TPS. The observations recorded on growth parameters showed that biochar had significant effect on growth of lettuce but no consistent effect on growth of TPS and single node cuttings. Among the biochar rates, 30 t ha^-1 had the greatest influence on overall growth of lettuce. The pH and electrical conductivity increased as the biochar rates increased in all experiments. These results provide an avenue for soil management system by using biochar as an amendment in horticultural crops. However, their verification in the field is important for specific recommendations.
基金Sponsored by Key Program of Sichuan Provincial Department of Education(12ZA149)
文摘With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results showed that under the stress of Pb (200 mg/kg) and Cd (20 mg/kg), growth of potato had no obvious difference with that in control group; under the stress of Pb ( 〉200 mg/kg) and Cd ( 〉20 mg/kg), potato growth suspended, and its physiological indexes such as dry weight, plant height, root number, root length were inhibited obviously. With the increasing mass fraction of Pb, relative electrical conductivity, MDA and proline of potato leaves showed the tendency of increase, and the results in high mass fraction treatments showed significant difference with those in the control group (P 〈 0.05 ), CAT, POD and SOD activity of leaves increased greatly with the increasing mass fraction. In the Pb stress treatment (200 mg/kg), relative electrical conductivity, MDA and proline of potato leaves showed no obvious difference with those in the control group, and the results in high mass fraction treatments were significantly higher than those in the control group ( P 〈 O. 05 ), CAT and SOD activity increased greatly with the increasing mass fraction ( P 〈 0.05 ). Under the stress of Pb and Cd, synthesis of soluble protein in potato leaves was strongly inhibited. The results showed that potato was tolerant to Pb and Cd of lower mass fraction, and damage of potato caused by Pb and Cd stress could be relieved by improving activity of antioxidase and reducing peroxidation of membrane lipid, and the tolerance reduced with the increase of stress.
文摘With the detoxicated seedling of a potato cultivation breed named “Mire” as the material, the effect of auxins CCC, 6-BA, and active carbon to microtubers of potato (Solanum tubersum L.) was investigated under the in-vitro circumstances. The result indicated the exogenous auxins improved the production and quality of microtubers of potatoes. The effect of induction can be described as CCC > CCC + 6-BA > 6-BA > CK, the number of microtubers in per flask is 8.17 > 7.67 > 7.29 > 5.46, and the number of large potatoes in per flask is 6.33 > 5.17 > 3.17 > 1. In addition, by adding 0.5‰ of active charcoal, the growth period was shortened from 25.0 days to 9.33 days on average, and the amount of larger potatoes increased 8.54%. These results benefited the growth of microtubers of potato.
文摘Two separate experiments were conducted to evaluate the success of the establishment and growth of micro-cuttings of potato (5 - 6 cm tall) in sand trays [38 cm (L) × 28 cm (W) × 7.5 cm (H) plastic trays] under controlled environment (22?C ± 2?C, 60 - 75 μmosm–1?s–1 light energy for 16 h daily). In the first experiment, micro-cuttings of potato cv. Diamant were planted at six populations (500, 600, 700, 800, 900 and 1000 cuttings per tray) in treated sand (sun dry, 1% formaldehyde, 0.2% Dithane M-45 and control). The mortality percentage of micro-cuttings was nil for sun dry sand while formaldehyde and dithane M-45 treated sand had 1% - 4% against 15% in the control with the highest population density. Mortality of micro-cuttings in formaldehyde and dithane M-45 treated sand trays were found not to be related to pathogenic organism rather toxic effect of these two chemicals. Micro-cuttings in Sun dry and control treatments showed better growth performance than these in chemically treated sand trays. In the second experiment, urea @ 1, 2 and 3 g per tray was applied as solid form after 15 days of planting the micro-cuttings and as liquid form @ 0.5, 1 and 2% solution sprayed in the micro-cuttings repeatedly after 15, 30, 45 and 60 days of planting. The micro-cuttings which received urea as solid state died within 2 - 3 days and 2% urea solution was also detrimental. Urea solution @ 0.5% found to be very effective for vegetative growth of micro-cuttings in sand trays. The control was also good for vegetative growth but at a slower rate.
文摘The study was carried out to determine the influence of sweet potato peels and cashew nut shell supplemented with Ocimum gratissium leaves, using growth indices and blood constituents by goats. Eighteen goats with an average weight of 8.11 kg and aged between 8 and 9 months old were allotted to three dietary treatments with six animals per treatment in a complete randomized design. The compared diets which comprised combination of sweet potato peels and cashew nut shell with concentrate respectively were in different ratios which included diet I (30:25:45), diet II (25:30:45) and diet III (20:35:45). Each goat also received 8 grams of Ocimum gratissium leaves as supplement in the diet. The results showed that average feed intake (6.42 kg) and fed conversion ratio (2.15) were highest in diet I and significantly different from other diets. Diet II had the highest significant (P 3/L), lymphocytes (56.01%), monocytes (1.06%), total protein (7.65 g/dl), albumin (3.58 g/dl), globulin (4.07 g/dl) and triglyceride (2.29 mmol/L). White blood cell (7.01 × 103/L), neutrophils (49.82%), cholesterol (42.34 mmol/L), creatnine (1.59 mg/dl) and urea (18.29 mg/dl) were (P < 0.05) best in diet III, whereas initial bodyweight, average daily weight gain, eosinophils and basophils were not significantly different (P < 0.05). It is concluded that diet II has the potential to enhance growth rate and blood constituents of goats.
基金supported by the Scientific Research and Innovation Plan for College Graduates of Jiangsu Province, China(CXZZ13_0521)
文摘Potato grows in most part of China, it achieves higher yield and better quality in Gansu Province than in others. With global warming, its growth duration has been prolonged and sowing date become earlier than before. Therefore, to regulate its sowing date and growing period is of great significance for better harvest. In this study, experiments were conducted with six sowing-date treatments of potato in Dingxi, which is in the Loess Plateau of central Gansu Province in Northwest China in 2010. The growth period, morphological index and change in yield and their relationships with temperature, precipitation, and other climatic factors were investigated for each treatment. Results show that the crop with different sowing dates experienced different climate conditions, leading to distinct growth duration, plant height, and leaf area index. The growth duration was shortened due to a delay in sowing date. For each 15-day delay in sowing, the growth duration was reduced by 12 days on average. A significant linear relationship was found between numbers of days either from seeding to emergence or from flowering to harvest and mean temperature over the corresponding period. Dry matter accumulation, tuber fresh weight, and final yield were all decreased because of insufficient cumulative temperature over the shorter growing periods. Marked differences in tuber yield were discovered among the six treatments of sowing date, the potato planted on May 27 giving the highest yield. The potato planted either earlier or later would produce invariably lower yield than the treatment of May 27. Late May therefore can be taken as the optimum sowing time of potato in this region because the crop can fully utilize thermal resource. We conclude that to postpone sowing time is a good practice for potato production to adapt to climate warming in the Loess Plateau of central Gansu, China.
文摘Different aspects of micropropagation through meristem culture for the production of virus indexed source plants, <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> tuberization and field evaluation of the </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> regenerated plants were studied on four commercial cultivars of potato (</span><i><span style="font-family:Verdana;">Solanum tuberosum</span></i><span style="font-family:Verdana;"> L.) viz., Diamant, Cardinal, Shilbilati and Lalpakri. The investigation was conducted at Rajshahi, Bangladesh from December 2010 to March 2012 to produce virus-free potato plantlets through meristem culture, shoot multiplications with root induction as well as their acclimatization and evaluation of morphological characters and tuber yield under field condition. Shoot tips of 25</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">30 day old field</span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">grown plants of above mentioned four cultivars were used for meristem isolation. After isolation, meristems of these varieties of potato were cultured on “M” shaped filter paper bridge in Murashige and Skoog (MS) liquid medium. Four different treatments of media formulations viz. 0.1 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, 0.1</span></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">, 0.5 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> and 0.5 mg/L KI</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">N + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> were used as plant growth regulators. From these formulations MS + 0.1 mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> was found to be the best for </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">primary establishment of meristem culture. The primar</span><span style="font-family:Verdana;">ily</span><span style="font-family:Verdana;"> established meristems were subcultured on to MS semisolid basal medium supplemented with four different treatment combinations of hormones viz. 0.5 mg/L BA</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">+ 1.0 mg/L IBA;0.1 mg/L KIN + 0.1 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;">;0.5 mg/L BA + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> and 0.5 mg/L KIN + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> were used to identify the suitable media compositions for shoot proliferation. Results showed that out of these four media treatments the formulation 0.5 mg/L BA + 0.5 mg/L GA</span><sub><span style="font-family:Verdana;">3</span></sub><span style="font-family:Verdana;"> was found to be the best suitable for shoot generation. Among the four cultivars of potato higher frequency of shoot proliferation (number of shoots/explant and longest shoot length) was observed in Diamant, though the highest shoot formation (76%) was recorded in Cardinal. Virus free </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> grown potato plantlets were ob</span><span style="font-family:Verdana;">tained through DAS-ELISA test and used substantially for m</span><span style="font-family:Verdana;">icro-propagation. After gradual acclimatization of rooted plantlets of four potato cultivars</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> they were transferred into the field for cultivation and established successfully. It was observed from the field study of </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> meristem-derived plantlets that there were no virus-affected plants. The virus-free exotic varieties were much superior in all vegetative attributes and yield compare</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> to those of indigenous varieties with producing potato plants of normal height. In contrast, the indigenous varieties took </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">longer time to tuber initiation and maturity, lower plant height and number of leaves per plant, </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">higher number of tubers but </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">lower amount of tuber weight per plant, and poorer tuber grade than the exotic varieties. However, the variety Cardinal exposed the best performances in the context of survival percentage of plantlets (90%), days to tuber initiation (DTI), </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">number of leaves per plant (NL), tuber weight per plant (343.40%) and the percentage of rich tuber grade.</span>
文摘This study consisted to evaluate the effects of ecological practices on the yield performance of the JEWEL of orange-fleshed sweet potato (Ipomoea batatas (L.) Lam). The study was conducted in a Sudano-Sahelian cropping system (Lantargou, eastern region of Burkina Faso). Agro-ecological practices consisted of the inputs of 3.20 t/ha of compost + 2.45 t/ha of wood ash (CO + WA);4.90 t/ha of wood ash (WA);6.40 t/ha of compost (CO) were compared to control with no inputs (T<sub>0</sub>). Each treatment was repeated four times. The crop management consisted of plowing, harrowing, raising of ridges with 40 cm height, burying of treatments, transplanting of cuttings, two weeding’s, and using of biopesticide called PIOL for crop protection. Composite soil samples per treatment were also collected at tuber harvest and analyzed to determine the effects of treatments on residual soil fertility. Results showed that the plant heights and diameters under the CO were significantly (P < 0.001) improved by 16% and 12% compared to T<sub>0</sub>. The WA treatment significantly increased the number of large tubers by 43% (P ≤ 0.01) compared to T<sub>0</sub>. Total tuber numbers, large tuber numbers and tuber yields of sweet potato under CO + WA were significantly (P < 0.001) and respectively improved by 27%, 50% and 31% compared to T<sub>0</sub>. All treatments increased soil organic matter, N, P and K contents, and reduced soil acidity compared with those obtained under T<sub>0</sub>. Soil K content was improved by 39% under CO + WA, and soil N content by 34% under WA compared to T<sub>0</sub>. Soil C/N ratio under CO + WA was reduced by 20% compared others treatments. But, the CO + WA treatment outperformed by improving residual soil N content by 38%, and the WA treatment by increasing soil K content by 50% compared to T<sub>0</sub>. In addition, soil pHH<sub>2</sub>O increased by 1.2 units under WA treatment compared to T<sub>0</sub>. As conclusion, the application of 6.4 t/ha of compost performed well to improve the vegetative growth of orange-fleshed sweet potato while the inputs of 3.2 t/ha of compost + 2.45 t/ha of wood ash were efficacy to significantly increase the tuber yields and improve the residual fertility of soil.