Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro as...Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro assay.Among them,FBCC-EP850 exhibited the most promising inhibitory activity.Further analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish larvae.Results:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/mL.FBCC-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish larvae.Treatment with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma cells.Additionally,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish larvae.Its anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling pathway.Conclusions:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders.展开更多
The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2...The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2,1%, 2.4%, 3.0%） of Na2CO3 and NaHCO3 mixtures were studied in a greenhouse of Northeast Forestry University, Harbin, China. The rate of electrolyte leakage （REL） and SOD （Superoxide dismutase） activity in leaves of different samples were determined. Results showed that the REL in leaves of U. pumila presented a slowly increasing trend at the salt concentrations less than 1.5%, which indicated that cell plasma membrane of U. pumila leaves had rather strong resistance to the injury of salt ion, and had a significant increase at the salt concentrations more than 1.5%. The SOD activities in leaves of U, pumila presented an increased trend at salt concentrations less than 1.5%, the growth of seedlings did not decline, and tress and leaves had no symptom of injury, while the salt concentrations exceeded 1.5%, SOD activities sharply decreased and REL increased promptly.展开更多
A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology ...A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology of Thermal Desorption-Gas Chromatography-Mass Spectrometry（TD-GC-MS）.The results indicated that the main components of VOC from heated needles and twigs are terpenoids.Most of these terpenoids are monoterpenes.Terpenoids account for 72.93% for the needles and 92.40% for the twigs of the total VOC,and their emission ratios are 61.200 μg·g-1 and 217.060 μg·g-1 respectively.Heated twigs can emit more terpenoids than heated needles because twigs had more volatile oils than needles.In actual fires,these large amounts of terpenoid emissions,especially the monoterpene emissions,have strong effects on fire behaviors that are not only in the initial stage but also in the fast propagation stage of fires.These flammable gases are capable of causing violent combustion and creating crown fires.In addition,if these gases accumulate in an uneven geographical area,there will be a possible for eruptive fires and/or fires flashover to occur.展开更多
Ficus (Moraceae) is a well-known group with specific pollination mutualisms, and hybridization is considered to be rare. Here, we report the presence of interspecific hybrids between Ficus pumila L. and F. thunbergii ...Ficus (Moraceae) is a well-known group with specific pollination mutualisms, and hybridization is considered to be rare. Here, we report the presence of interspecific hybrids between Ficus pumila L. and F. thunbergii Maxim. on Okinoshima, a small island offshore of Shikoku, western Japan. AFLP (amplified fragment length polymorphism of genomic DNA) data suggested that more than one-fourth of individuals of morphological F. pumila were assigned as intermediate genotypes, suggesting hybrids. The hybridization between the two species was introgressive and unidirectional from F. thunbergii to F. pumila. The findings of this study, combined with other previous reports, suggest that the breakdown of mutualistic systems can occur in isolated populations such as those on islands.展开更多
[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeabili...[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeability and carrageenan-induced toe swelling were established to evaluate the effects of extracts from different parts of F.pumila L.on inflammation.The inflammatory model of ear swelling in mice with bilateral adrenalectomy induced by xylene was established to determine whether its anti-inflammatory effect depends on hypothalamus-pituitary-adrenal axis(HPAA)system.[Results]Compared with the blank control group,except that there was no statistical significance in the low dose group(P>0.05),the high and middle dose groups of extracts from different parts of F.pumila L.had significant inhibitory effects on xylene-induced ear swelling in mice.The high,middle and low dose groups of F.pumila L.extract could significantly increase the celiac capillary permeability of mice induced by glacial acetic acid,and significantly inhibit the toe swelling induced by carrageenan.Compared with the blank control group,the high and middle dose groups of extracts from different parts of F.pumila L.could significantly inhibit xylene-induced ear swelling and carrageenan-induced toe swelling in bilateral adrenalectomy mice.[Conclusions]The extracts from different parts of F.pumila L.had anti-inflammatory activity,and its anti-inflammatory effect was independent of hypothalamus-pituitary-adrenal axis(HPAA)system.展开更多
Elm （Ulmus pumila）, widely distributed in the north temperate zone, contributes to a special savanna-like woodland in typical grassland region in the northeastern China. This woodland performs a variety of ecologica...Elm （Ulmus pumila）, widely distributed in the north temperate zone, contributes to a special savanna-like woodland in typical grassland region in the northeastern China. This woodland performs a variety of ecological functions and environmental signifi- cance, such as decreasing soil erosion, stabilizing sand dunes, preserving species diversity. However, in the last approximate 30 years, the species composition, productivity and distribution area of elm woodland has decreased severely. A series of studies have been carried out to find out whether the climate changes or human disturbances caused the degradation of elm woodland and how these factors af- fected elm woodland. In this study, undisturbed, plowing and grazing elm woodland were investigated in 1983 and 2011 by using Point-Centered Quarter method. The relationship between vegetation changes and environmental factors was analyzed by Bray-Curtis ordination. The results show that in 2011, species diversity and understory productivity of undisturbed elm woodland decrease slightly compared to those of undisturbed elm woodland in 1983. However, nearly 60% of the species is lost in the plowing and grazing elm woodland relative to the species undisturbed elm woodland in 1983. Interestingly, plowing stimulates the growth of elm and certain understory species through furrowing soil and accelerating soil nutrient turnover rate. Grazing disturbance not only leads to species loss and productivity decrease, but also induces changes in elm growth （small, short and twisted）. The mean age of the elm was 29 -4- 2 yr in undisturbed and plowing elm woodland, while only 15 yr in the grazing elm woodland. The results of Bray-Curtis ordination analysis show that all sample stands clustered to three groups： Group I including the undisturbed sample stands of 83UE （undisturbed elm wood- land in 1983） and l lUE （undisturbed elm woodland in 2011）; Group II including sample stands of PE （elm woodland disturbed by plowing）; Group III including samples stands of GE （elm woodland disturbed by grazing）. The results indicate that the long time distur- bance of the plowing and grazing have converted elm woodland to different community types. Climate change is not the primary reason causing the degradation of elm woodland, but plowing and grazing disturbance. Both plowing and grazing decrease the vegetation composition and species diversity. Grazing further decreases vegetation productivity and inhibits the growth of elm tree. Therefore, we suggest that reasonable plowing and exclusive grazing would be favorable for future regeneration of degraded elm woodland.展开更多
This study was to establish the micropropagation system of Labisia pumila （Bl.） F. Vill. and explore the feasibility of mass production of its multiple shoots by bioreactor culture. [Method] The effects of cytokini...This study was to establish the micropropagation system of Labisia pumila （Bl.） F. Vill. and explore the feasibility of mass production of its multiple shoots by bioreactor culture. [Method] The effects of cytokinin or cytokinin/auxin mixture solution at different concentrations on its shoot growth and propagation by nodal segment culture were tested. In order to mass-proliferate its shoots, multiple shoots were suspension-cultured on Murashige and Skoog （MS） liquid medium in the bioreactor. [Results] Thidiazuron （TDZ） showed the highest cytokinin-like activity and the number of its shoots at the TDZ concentration of 0.1 mg/L was the maximum up to 21.5 shoots per explant, without the TDZ effect on shoot elongation, but zeatin signifcantly promoted the shoot elongation and each explant grew 6.8 relatively long shoots at 5.0 mg/L of zeatin. There was no signifcant effect on shoot propagation on induction medium supplemented with auxin. By inoculating 10 g/L （260 shoots） of multiple shoots into 3 L airlift bioreactor containing 2 L MS liquid medium supplemented with 0.1 mg/L TDZ for solution culture, 6 100 multiple shoots with a fresh weight of 183.13 g were harvested. After explant regeneration was conducted in MS solid medium, the regenerated plants were transplanted into flowerpot and the morphologically normal and healthy plants were obtained. [Conclusion] The development of bioreactor technology was conducive to the high efficiency propagation of L. pumila shoot.展开更多
Camptothecin(CPT) is an anticancer pentacyclic quinoline alkaloid widely used to treat cancer patients worldwide. However, the biosynthetic pathway and transcriptional regulation of camptothecin are largely unknown. O...Camptothecin(CPT) is an anticancer pentacyclic quinoline alkaloid widely used to treat cancer patients worldwide. However, the biosynthetic pathway and transcriptional regulation of camptothecin are largely unknown. Ophiorrhiza pumila, the herbaceous plant from the Rubiaceae family, has emerged as a model plant for studying camptothecin biosynthesis and regulation. In this study, a high-quality reference genome of O. pumila with estimated size of ~456.90Mb was reported, and the accumulation level of camptothecin in roots was higher than that in stems and leaves. Based on its spatial distribution in the plant, we examined gene functions and expression by combining genomics with transcriptomic analysis.Two loganic acid O-methyltransferase(OpLAMTs)were identified in strictosidine-producing plant O.pumila, and enzyme catalysis assays showed that OpLAMT1 and not OpLAMT2 could convert loganic acid into loganin. Further knock-out of OpL AMT1expression led to the elimination of loganin and camptothecin accumulation in O. pumila hairy roots.Four key residues were identified in OpLAMT1 protein crucial for the catalytic activity of loganic acid to loganin. By co-expression network, we identified a NAC transcription factor, OpNAC1, as a candidate gene for regulating camptothecin biosynthesis.Transgenic hairy roots and biochemical assays demonstrated that OpNAC1 suppressed OpLAMT1 expression. Here, we reported on two camptothecin metabolic engineering strategies paving the road for industrial-scale production of camptothecin in CPT-producing plants.展开更多
基金supported by the Korea Environmental Industry&Technology Institute through Project to Make Multi-ministerial National Biological Research Resources more Advanced funded by Korea Ministry of Environment(RS-2021-KE001783).
文摘Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro assay.Among them,FBCC-EP850 exhibited the most promising inhibitory activity.Further analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish larvae.Results:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/mL.FBCC-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish larvae.Treatment with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma cells.Additionally,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish larvae.Its anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling pathway.Conclusions:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders.
文摘The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2,1%, 2.4%, 3.0%） of Na2CO3 and NaHCO3 mixtures were studied in a greenhouse of Northeast Forestry University, Harbin, China. The rate of electrolyte leakage （REL） and SOD （Superoxide dismutase） activity in leaves of different samples were determined. Results showed that the REL in leaves of U. pumila presented a slowly increasing trend at the salt concentrations less than 1.5%, which indicated that cell plasma membrane of U. pumila leaves had rather strong resistance to the injury of salt ion, and had a significant increase at the salt concentrations more than 1.5%. The SOD activities in leaves of U, pumila presented an increased trend at salt concentrations less than 1.5%, the growth of seedlings did not decline, and tress and leaves had no symptom of injury, while the salt concentrations exceeded 1.5%, SOD activities sharply decreased and REL increased promptly.
基金supported by National Natural Science Foundation of China (30872037, 31070587)Open Project Program of State Key Laboratory of Fire Science, University of Science and Technology of China (HZ2008-KF08)
文摘A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology of Thermal Desorption-Gas Chromatography-Mass Spectrometry（TD-GC-MS）.The results indicated that the main components of VOC from heated needles and twigs are terpenoids.Most of these terpenoids are monoterpenes.Terpenoids account for 72.93% for the needles and 92.40% for the twigs of the total VOC,and their emission ratios are 61.200 μg·g-1 and 217.060 μg·g-1 respectively.Heated twigs can emit more terpenoids than heated needles because twigs had more volatile oils than needles.In actual fires,these large amounts of terpenoid emissions,especially the monoterpene emissions,have strong effects on fire behaviors that are not only in the initial stage but also in the fast propagation stage of fires.These flammable gases are capable of causing violent combustion and creating crown fires.In addition,if these gases accumulate in an uneven geographical area,there will be a possible for eruptive fires and/or fires flashover to occur.
文摘Ficus (Moraceae) is a well-known group with specific pollination mutualisms, and hybridization is considered to be rare. Here, we report the presence of interspecific hybrids between Ficus pumila L. and F. thunbergii Maxim. on Okinoshima, a small island offshore of Shikoku, western Japan. AFLP (amplified fragment length polymorphism of genomic DNA) data suggested that more than one-fourth of individuals of morphological F. pumila were assigned as intermediate genotypes, suggesting hybrids. The hybridization between the two species was introgressive and unidirectional from F. thunbergii to F. pumila. The findings of this study, combined with other previous reports, suggest that the breakdown of mutualistic systems can occur in isolated populations such as those on islands.
文摘[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeability and carrageenan-induced toe swelling were established to evaluate the effects of extracts from different parts of F.pumila L.on inflammation.The inflammatory model of ear swelling in mice with bilateral adrenalectomy induced by xylene was established to determine whether its anti-inflammatory effect depends on hypothalamus-pituitary-adrenal axis(HPAA)system.[Results]Compared with the blank control group,except that there was no statistical significance in the low dose group(P>0.05),the high and middle dose groups of extracts from different parts of F.pumila L.had significant inhibitory effects on xylene-induced ear swelling in mice.The high,middle and low dose groups of F.pumila L.extract could significantly increase the celiac capillary permeability of mice induced by glacial acetic acid,and significantly inhibit the toe swelling induced by carrageenan.Compared with the blank control group,the high and middle dose groups of extracts from different parts of F.pumila L.could significantly inhibit xylene-induced ear swelling and carrageenan-induced toe swelling in bilateral adrenalectomy mice.[Conclusions]The extracts from different parts of F.pumila L.had anti-inflammatory activity,and its anti-inflammatory effect was independent of hypothalamus-pituitary-adrenal axis(HPAA)system.
基金Under the auspices of the Seventh Framework Programme(European Commission,No.226818)National Natural Science Foundation of China(No.31070294,31072070,31100331)
文摘Elm （Ulmus pumila）, widely distributed in the north temperate zone, contributes to a special savanna-like woodland in typical grassland region in the northeastern China. This woodland performs a variety of ecological functions and environmental signifi- cance, such as decreasing soil erosion, stabilizing sand dunes, preserving species diversity. However, in the last approximate 30 years, the species composition, productivity and distribution area of elm woodland has decreased severely. A series of studies have been carried out to find out whether the climate changes or human disturbances caused the degradation of elm woodland and how these factors af- fected elm woodland. In this study, undisturbed, plowing and grazing elm woodland were investigated in 1983 and 2011 by using Point-Centered Quarter method. The relationship between vegetation changes and environmental factors was analyzed by Bray-Curtis ordination. The results show that in 2011, species diversity and understory productivity of undisturbed elm woodland decrease slightly compared to those of undisturbed elm woodland in 1983. However, nearly 60% of the species is lost in the plowing and grazing elm woodland relative to the species undisturbed elm woodland in 1983. Interestingly, plowing stimulates the growth of elm and certain understory species through furrowing soil and accelerating soil nutrient turnover rate. Grazing disturbance not only leads to species loss and productivity decrease, but also induces changes in elm growth （small, short and twisted）. The mean age of the elm was 29 -4- 2 yr in undisturbed and plowing elm woodland, while only 15 yr in the grazing elm woodland. The results of Bray-Curtis ordination analysis show that all sample stands clustered to three groups： Group I including the undisturbed sample stands of 83UE （undisturbed elm wood- land in 1983） and l lUE （undisturbed elm woodland in 2011）; Group II including sample stands of PE （elm woodland disturbed by plowing）; Group III including samples stands of GE （elm woodland disturbed by grazing）. The results indicate that the long time distur- bance of the plowing and grazing have converted elm woodland to different community types. Climate change is not the primary reason causing the degradation of elm woodland, but plowing and grazing disturbance. Both plowing and grazing decrease the vegetation composition and species diversity. Grazing further decreases vegetation productivity and inhibits the growth of elm tree. Therefore, we suggest that reasonable plowing and exclusive grazing would be favorable for future regeneration of degraded elm woodland.
基金Supported by World Class 300 R&D Project from Ministry of Knowledge Economy of Korea(10043192)~~
文摘This study was to establish the micropropagation system of Labisia pumila （Bl.） F. Vill. and explore the feasibility of mass production of its multiple shoots by bioreactor culture. [Method] The effects of cytokinin or cytokinin/auxin mixture solution at different concentrations on its shoot growth and propagation by nodal segment culture were tested. In order to mass-proliferate its shoots, multiple shoots were suspension-cultured on Murashige and Skoog （MS） liquid medium in the bioreactor. [Results] Thidiazuron （TDZ） showed the highest cytokinin-like activity and the number of its shoots at the TDZ concentration of 0.1 mg/L was the maximum up to 21.5 shoots per explant, without the TDZ effect on shoot elongation, but zeatin signifcantly promoted the shoot elongation and each explant grew 6.8 relatively long shoots at 5.0 mg/L of zeatin. There was no signifcant effect on shoot propagation on induction medium supplemented with auxin. By inoculating 10 g/L （260 shoots） of multiple shoots into 3 L airlift bioreactor containing 2 L MS liquid medium supplemented with 0.1 mg/L TDZ for solution culture, 6 100 multiple shoots with a fresh weight of 183.13 g were harvested. After explant regeneration was conducted in MS solid medium, the regenerated plants were transplanted into flowerpot and the morphologically normal and healthy plants were obtained. [Conclusion] The development of bioreactor technology was conducive to the high efficiency propagation of L. pumila shoot.
基金supported by the Major Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province (2021C02074)National Natural Science Foundation of China (82003889, 31571735, 82073963, 81522049)+5 种基金National Key Research and Development Program of China (2018YFC1706203)Zhejiang Provincial Natural Science Foundation of China (LQ21H280004, LY20H280008)Zhejiang Provincial Ten Thousands Program for Leading Talents of Science and Technology Innovation (2018R52050)Zhejiang Provincial Program for the Cultivation of High-level Innovative Health TalentsResearch Project of Zhejiang Chinese Medical University (2021JKZDZC06)Opening Project of Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine (2021E10013)。
文摘Camptothecin(CPT) is an anticancer pentacyclic quinoline alkaloid widely used to treat cancer patients worldwide. However, the biosynthetic pathway and transcriptional regulation of camptothecin are largely unknown. Ophiorrhiza pumila, the herbaceous plant from the Rubiaceae family, has emerged as a model plant for studying camptothecin biosynthesis and regulation. In this study, a high-quality reference genome of O. pumila with estimated size of ~456.90Mb was reported, and the accumulation level of camptothecin in roots was higher than that in stems and leaves. Based on its spatial distribution in the plant, we examined gene functions and expression by combining genomics with transcriptomic analysis.Two loganic acid O-methyltransferase(OpLAMTs)were identified in strictosidine-producing plant O.pumila, and enzyme catalysis assays showed that OpLAMT1 and not OpLAMT2 could convert loganic acid into loganin. Further knock-out of OpL AMT1expression led to the elimination of loganin and camptothecin accumulation in O. pumila hairy roots.Four key residues were identified in OpLAMT1 protein crucial for the catalytic activity of loganic acid to loganin. By co-expression network, we identified a NAC transcription factor, OpNAC1, as a candidate gene for regulating camptothecin biosynthesis.Transgenic hairy roots and biochemical assays demonstrated that OpNAC1 suppressed OpLAMT1 expression. Here, we reported on two camptothecin metabolic engineering strategies paving the road for industrial-scale production of camptothecin in CPT-producing plants.
