利用26S r DNA D1/D2区序列分析法鉴定了内蒙古牧区传统开菲尔发酵液中的酵母菌,为筛选可发酵特色乳酒的酵母菌奠定基础。对所分离纯化得的酵母菌进行菌落特征和细胞显微形态区分,在形态鉴定基础上,选择代表菌进行26S r DNA D1/D2区序...利用26S r DNA D1/D2区序列分析法鉴定了内蒙古牧区传统开菲尔发酵液中的酵母菌,为筛选可发酵特色乳酒的酵母菌奠定基础。对所分离纯化得的酵母菌进行菌落特征和细胞显微形态区分,在形态鉴定基础上,选择代表菌进行26S r DNA D1/D2区序列分子鉴定。结果表明,共分离到36株酵母菌,形态聚类为5类,经DNA提取、26S r DNA D1/D2区PCR扩增、酶切、基因序列分析和同源性比对,鉴定为5种分子类型的酵母菌,分别为胶红酵母菌(Rhodotorula mucilaginosa)、诞沫假丝酵母菌(Candida zeylanoides)、发酵毕赤酵母菌(Pichia fermentans)、酿酒酵母菌(Saccharomyces cerevisiae)、有孢圆酵母菌(Torulaspora delbrueckii)。传统开菲尔发酵液中分离到的酿酒酵母,具有可发酵特色乳酒的潜质。展开更多
The phylogenetic position of the subfamily Coelotinae, which belongs to Amaurobiidae currently but Agelenidae formerly, has remained controversial. The sequences of 28S and 18S rDNA fragments were obtained from six sp...The phylogenetic position of the subfamily Coelotinae, which belongs to Amaurobiidae currently but Agelenidae formerly, has remained controversial. The sequences of 28S and 18S rDNA fragments were obtained from six species of spiders representing Ageleninae, Amaurobiinae, Coelotinae and Clubionidae. Neighbor-joining (NJ), maximum parsimony (MP), maximum-likelihood (ML) and Bayesian (BI) analyses generated identical tree topologies. All trees from the 28S rDNA data derived from neighbor-joining, maximum parsimony, maximum-likelihood and Bayesian analyses support the Coelotinae as the sister group of the Ageleninae, and strongly support the Coelotinae + Ageleninae are the sister group of the Amaurobiinae. The topology in the trees inferred from 18S data is similar to those of 28S rDNA but with lower boostrap support. The results indicate that Coelotinae is probably a subfamily of the family Agelenidae. In addition, the close relationship between Asiacoelotes and Draconarius of the subfamily Coelotinae, Agelena and Alloagelena of the subfamily Ageleninae is strongly supported by the results of analyses of the 28S rDNA sequences. This indicates that the sequence could be used as a molecular marker in the study of phylogenetic relationships among the genera of spiders.展开更多
文摘利用26S r DNA D1/D2区序列分析法鉴定了内蒙古牧区传统开菲尔发酵液中的酵母菌,为筛选可发酵特色乳酒的酵母菌奠定基础。对所分离纯化得的酵母菌进行菌落特征和细胞显微形态区分,在形态鉴定基础上,选择代表菌进行26S r DNA D1/D2区序列分子鉴定。结果表明,共分离到36株酵母菌,形态聚类为5类,经DNA提取、26S r DNA D1/D2区PCR扩增、酶切、基因序列分析和同源性比对,鉴定为5种分子类型的酵母菌,分别为胶红酵母菌(Rhodotorula mucilaginosa)、诞沫假丝酵母菌(Candida zeylanoides)、发酵毕赤酵母菌(Pichia fermentans)、酿酒酵母菌(Saccharomyces cerevisiae)、有孢圆酵母菌(Torulaspora delbrueckii)。传统开菲尔发酵液中分离到的酿酒酵母,具有可发酵特色乳酒的潜质。
文摘The phylogenetic position of the subfamily Coelotinae, which belongs to Amaurobiidae currently but Agelenidae formerly, has remained controversial. The sequences of 28S and 18S rDNA fragments were obtained from six species of spiders representing Ageleninae, Amaurobiinae, Coelotinae and Clubionidae. Neighbor-joining (NJ), maximum parsimony (MP), maximum-likelihood (ML) and Bayesian (BI) analyses generated identical tree topologies. All trees from the 28S rDNA data derived from neighbor-joining, maximum parsimony, maximum-likelihood and Bayesian analyses support the Coelotinae as the sister group of the Ageleninae, and strongly support the Coelotinae + Ageleninae are the sister group of the Amaurobiinae. The topology in the trees inferred from 18S data is similar to those of 28S rDNA but with lower boostrap support. The results indicate that Coelotinae is probably a subfamily of the family Agelenidae. In addition, the close relationship between Asiacoelotes and Draconarius of the subfamily Coelotinae, Agelena and Alloagelena of the subfamily Ageleninae is strongly supported by the results of analyses of the 28S rDNA sequences. This indicates that the sequence could be used as a molecular marker in the study of phylogenetic relationships among the genera of spiders.
