In-situ Elimination Effect on Heavy Metals in Contaminated Soil from the Mining Area by Ramie

[Objective] The aim was to study on in-situ elimination effect on heavy metals in soil of the mining area by ramie （Boehmeria nivea （L.） Gaud.）. [Methods] Based on Xiangzhu No.3 and Zhongzhu No.1, we conducted research on heavy metals contents of plants growing in soil of Qibao Mountain orefield in Liuyang, Hunan Province, and on characteristics of enrichment and transfer of heavy metals （Cu, Pb, Cd, Zn） under influence of the two ramie species. [Result] It was concluded that trend of Cu content in different parts of ramie was as follows： rootskinleafbone; trend of Pb was rootleafskinbone; trend of Cd was rootskinboneleaf; the trend of Zn was rootskinboneleaf. In farmland A （with low content of heavy metal）, for per square meter of plough horizon, effect of Zhong 1 on heavy metals transferring volume and the period for restoration of the soil into national standard one （Category Ⅲ of Environmental Quality Standard for Soil） have been concluded. Specifically, for Cu, the corresponding values were 3 404.44 mg and 8.59 y, respectively; for Pb, the values were 3 638.5 mg and 13.52 y; for Cd, the values were 720.48 mg and 1.49 y; for Zn, the values were 37 324.8 mg and 0.67 y. [Conclusion] Soil contaminated by Cu, Pb, Cd, and Zn in orefield can be rapidly restored by growing ramie.

Analysis of Climatic Factors Causing Yield Difference in Ramie among Different Eco-regions of Yangtze Valley

[Objective] This study was to investigate the climatic factors affecting the regional eco-adaptability of ramie.[Method] Five experimental sites of different eco-types in ramie growing regions in Yangtze Valley were set up to investigate the yield of Zhongzhu No.2,based on which we assessed the adaptability of Zhongzhu No.2 to various eco-regions and further analyzed the climatic factors causing the difference in eco-adaptability.[Result] Ramie yield varied largely among various experimental sites and the yield difference appeared to be well repeatable,with a differential value between the maximum and the minimum reaching 2.3 kg/20 m2.Analysis of the yield data,together with climatic factors in various ecological regions showed that yield of Zhongzhu No.2 was significantly correlated with rainfall,sunshine hours,relative humidity during its growing period.Of the climatic factors,sunshine hours and rainfall can positively promote yield increase,while relative humidity negatively regulate ramie yield.[Conclusion] The climatic indices,including rainfall,sunshine duration and relative humidity are a key factor causing yield difference in ramie among different regions of Yangtze Valley.

THE INFLUENCE OF COMBING ROLLER SPEED ON PURE RAMIE NOIL ROTOR YARN QUALITY AND SPINNING PERFORMANCE

This paper reports the optimum design of combing roller speed with reference to yielding 6-13 Ne(98.4—45.4 tex) pure ramie noil rotor-spun yarns. The universal rotatable composite design is adopted to get the highly precise regression equations, in which the variables are combing roller speed and yarn linear density considered to be the main technological parameters of combing roller and, the responses are the evaluation indexes concerning spinning stability, fiber length distribution of fibre ring from rotor groove and yarn properties. Based on these regression equations, the contours are plotted to analyse the influences of the parameters on the evaluation indexes; the optimization multicriteria mathematical model is simultaneously established to obtain the optimum parameters with the aid of Object Programming Approach along with Constrained Random Ray Method. Finally, an experiment is carried out to further test the acceptance of the calculated optimum values. It is shown that, for any yarn linear density within 6—13 Ne (98.4—45.4 tex), combing roller speed varying from 5000 to 9150 r/min affects content of extra-long fibre in fibre ring from rotor groove and yarn properties but does not significantly influence spinning stability; 7075 r/min, at which the SAQ-12 type of saw-toothed combing roller runs, is recommended to be the optimum combing roller speed for producing 6—13 Ne (98.4—45.4 tex) pure ramie noil yarns, where a compromise is achieved among the lowest content of extra-long fibre in fibre ring, the best spinning stability and the best yarn properties.

SOME END-USES OF PURE RAMIE NOIL ROTOR YARNS

In the previous studies, the optimum technological combination has been obtained for producing coarse pure ramie noil yarns on rotor spinning system currently. Because of no yarn properties standard available at present for suchlike yarns, it is considered necessary to analyse the acceptability of coarse pure ramie noil rotor-spun yarns in terms of yarn properties before their products being developed. By the aid of a hypothetical concept "pure ramie noil ring-spun yarn", this paper draws the conclusion that the coarse pure ramie noil rotor-spun yarns are condsidered acceptable to a great extent; furthermore, it introduces 5 kinds of woven fabrics and 3 kinds of knitted fabrics successfully designed and developed from those yarns.

Mechanism of exogenous selenium alleviates cadmium induced toxicity in Bechmeria nivea(L.) Gaud(Ramie)

The protective role of exogenously supplied selenium （Se^6＋） on Bechmeria nivea （L.） Gaud （Ramie） subjected to cadmium （Cd） stress was studied in vitro, and the mechanism was discussed by investigating plant growth, malondialdehyde （MDA）, activity of antioxidative enzymes and DNA methylation pattern. Plants grown in hydroponic culture were supplied with spraying Se （selenate, 1.2μmol/L） and cadmium （Cd（NO3）2, 0, 3, 6 and 9 mg/L）, individually or simultaneously. At low Se spraying levels, SOD activity was increased by 35.34%, 43.18%, 3.63%under 3, 6 or 9 mg/L cadmium contents, POD was increased by 12.45%, 14.14%, 3.27%, and the level of DNA methylation was decreased by 10.70%, 18.18%and 15.59%, respectively. The results confirmed that spraying low Se on ramie leaves could enhance the activity of SOD and POD, and regulate DNA methylation in ramie leaves.

Genetic Relation Analysis on Ramie[Boehmeria nivea(L.)Gaud.]Inbred Lines by SRAP Markers

The objective of this article is to reveal the variations of ramie inbred lines in DNA level and discuss their molecular background to provide a theoretical basis for ramie cross breeding. In the present study, the genetic relationships among 33 inbred line accessions and two wild types that originated from China and Brazil were estimated using sequence-related amplified polymorphism （SRAP） markers. The results showed that 33 out of 81 primer combinations turned out to be polymorphic and 332 polymorphism bands were obtained. On the basis of the appearance of the markers, the genetic relationships were analyzed using unweighted pair-group method of arithmetic average cluster analysis （UPGMA）, and the genetic Jaccard similarity coefficients were calculated. The inbred-lines originating from China and Brazil formed a cluster suggesting a possibility that the Brazilian cultivars could have developed from cultivars introduced from China. Within ramie inbred-lines, the groupings also indicated that the greatest genetic relationship among cultivars was correlated to the region of origin of cultivars. The results provided the evidence that SRAP was an efficient approach, suitable for taxonomic analysis of ramie inbred lines, To the authors＇ knowledge, this is the first application of SRAP marker on the systematics of ramie inbred lines.

Effect of Planting Density and Fertilizer Application on Fiber Yield of Ramie (Boehmeria nivea)

As the most important cultural practices for ramie （Boehmeria nivea） production, the single effects of plant density and nitrogen （N）, phosphorus （P）, potassium （K） fertilization on yield are well documented. To achieve the high yield and quality of ramie fiber, it is principal to quantify the fertilizer dosage for ramie sustainable production. A central composite design （CCD） was adopted with three replications, for a two-year field experiment （2008-2009） in the Yangtze River Valley of China. The aim was to evaluate both the individual and combined effects of plant density and N, P, and K fertilization on yield and fiber quality. The effects of the four factors on yield and quality of ramie cultivar Huazhu 4 were tested. Then mathematical models of the relationship among the four factors affecting ramie production and quality were established and analyzed to optimize the four factors, and used to establish optimum cultivation methods for the elite cultivar suitable to this area of the Yangtze River Valley. Supplements of N （X2）, P 0（3） and K 0（4）, and the density （X0 with N （X2） interaction significantly influenced strength in tests of mature ramie. The influence on fiber yield in the two year tests by the four factors ranked as follows： X3〉X4〉X~〉X2. We established the optimization technique attaining yield of 2 600 kg ha-~ in the tests as following： density of 28 350-31 650 plants ha-1, and supplements of N, P and K of 363-387, 98.58-105.48 and 280.20- 319.8 kg ha-1, respectively. It was concluded that nitrogen mostly improved plant growth and fiber yield while potassium had discernible effects on fiber quality.

Chemical Composition and Nutritive Value of Ramie Plant [Boehmeria nivea （L.） Gaud] and Its By-Products from the Textile Industry as Feed for Ruminants

In order to verify the possible use of ramie as feed for ruminants, a study was undertaken on the chemical and nutritional evaluation of different parts of this plant. Four stocks of samples picked up in two different periods （summer and autumn） were analyzed. The standard chemical composition was determined on the whole plant and on its parts, the study was particularly focused on leaves and tops, not used in fibre production and available as livestock feed. Organic matter enzymic digestibility （OMD） was determined on all the samples by a double step method based on cellnlolytic andproteolytic enzymes. The chemical analysis showed good crude proteins content in leaves, tops and whole plant： 17.00 ~ 1.52%, 15.25 ~ 0.77% and 11.79 ~ 3.32% on dry matter （DM） basis respectively and good acid （ADF） and neutral （NDF） detergent fibre fractions. Lignin and ash resulted high in all plant parts and organic matter （OM） was consequently low, ash analysis showed high calcium concentration, especially in leaves, with values exceeding 4% of din, whereas the other micro （except the lead） and macroelements were in a normal range. OMD and energy values resulted poor in leaves, tops and in the whole plant and lower than the other forages commonly used as feed for runninants, thus the utilisation of ramie plant and its by-products requests further investigations.

Mechanical and Thermal Properties of Apocynum and Ramie Fiber Mat Reinforced Polylactic Acid Composites

With the increasing awareness of environmental protection and rational utilization of resources,natural fiber reinforced composites have shown broad development prospects.Apocynum fiber,known as the“king of wild fiber”,not only has moisture absorption,air permeability,and good mechanical properties but also has many health-related advantages such as antibacterial properties.In this study,four types of needle-punched Apocynum fiber and ramie fiber mat reinforced polylactic acid(PLA)composites were fabricated.Mechanical and thermal properties of the composites were tested and analyzed.The results showed that compared with those of the ramie fiber finish needle-punched mat reinforced composites,the tensile strength and the tensile modulus of Apocynum fiber finish needle-punched mat reinforced composites had increased by 15.3%and 60.1%,respectively.In comparison,the bending strength and the bending modulus were decreased by 21.8%and 7.6%,respectively.Moreover,compared with the Apocynum fiber finish needled-punched mat reinforced composites and the ramie fiber finish needle-punched mat reinforced composites,the Apocynum 50/ramie 50 finish needle-punched mat reinforced composites had the best tensile and bending properties.The after-fracture morphology was detected by a scanning electron microscope(SEM).The thermal properties of the composites were also characterized.It was found that the thermal properties of the four types of composites showed very similar behaviors.

Study on the Relationship Between Sex Differentiation and Ethylene in Ramie

To explore the role of ethylene in sexual determination in ramie, the ethylene release rates in ramie stem apex of different sex, bud of the same node in female and hermaphrodite ramie in the second crop, and single inflorescence of different sex in hermaphrodite ramie were measured by gas chromatography. Effects of two ethylene inhibitors on sex expression in ramie were investigated. The ethylene release rate of stem apex was higher in the second crop or female ramie than that in the third crop or hermaphrodite ramie during growth. Although ethylene release rates between lower nodes and higher nodes were little different, it was lower in the middle nodes in hermaphrodite ramie. The ethylene release rates were higher in the higher nodes of female ramie at the second crop. At the third crop, the ethylene release rates were higher in lower nodes, lower in middle nodes and then highest in higher nodes in female ramie. However, an opposite ethylene release pattern was observed in hermaphrodite ramie. The ethylene release rate was higher in female flower than male flower and mixed inflorescence in hermaphrodite ramie. The male flower could be distinctly induced by AVG （aminooethoxyvinylglycine）. The node of the first male flower, percentage of female flowers and ratio of female flowers to male in ramie were evidently depressed by AVG in contrast to water. The percentage of mixed male and female flowers was also increased and the percentage of female flower decreased by spraying AgNO3. There was a close relationship between sexual differentiation and ethylene release rate in ramie. The female ramie could be induced by high ethylene release rate. The female flower could be inhibited by AVG and AgNO3. AVG at a concentration of 300 mg L^-1 was most effective.

Effects of Nitrogen Application on Osmotic Adjustmentof Ramie (Boehmeria nivea L.) with Different NitrogenEfficiency

Ramie (Boehmeria nivea L.) is one of the most important fiber crops and biomass materials. However, previous studies showed that ramie presented a very low nitrogen agronomy efficiency (NAE, 23.2%~27.8%) in traditional farming, and the nitrogen fertilizer was applied excessively in ramie field. Plant osmotic adjustment (OA) responses to environmental stresses positively and exhibits improvements in plant tolerance. Whereas results varied due to the complexity of plant-environment interactions and lack of insights of specific species. In order to improve ramie production through osmoregulation, our current study investigated the role of nitrogen application and osmotic adjustment in improving the growth and yield in two varieties of ramie (H2000-03 and Ceheng Jiama) with contrasting nitrogen use efficiency (NUE) grown at 5 different N rates including N0, N6, N9, N12 and N15;0, 6, 9, 12 and 15 mmol/L N, respectively. The results showed that ramie adapted to different nitrogen rates through OA and significant differences of osmolyte content between varieties only presented at the particular growth stage. Obvious inflexion of yield, osmolyte content involving proline, soluble protein (SP), soluble sugar (SS) and malonaldehyde (MDA);nitrogen sensitive index (NSI) and comprehensive evaluation (D) in both varieties were observed. Our results recommended that the overall lifting of OA at a lower N level and at the proper growth stage would be a reasonable approach for improving ramie NUE.

Cloning and Expression of Key Enzyme Gene GalAT in Ramie Pectin Biosynthesis

To isolate the cDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea （L.） Gaud], and thus to understand the expression of GalAT gene in different tissues of ramie, degenerate primer was designed according to GalAT conserved sequence in other species reported, and the cDNA sequence of GalAT gene from ramie variety Zhongzhu 1 was cloned by RT-PCR method based on the degenerate primer. The cDNA revealed a 986-bp in length which encoded 328 amino acids. The cDNA sequence and putative amino acid sequence of GalAT shared high identity with previously reported Arabidopsis thaliana GA UT4 （GalAT） as 77 and 83%, respectively. Molecular evolution analysis showed that the putative amino acid sequence and Arabidopsis thaliana GAUT4 gathered to a same group. Real-time quantitative PCR analysis showed that GalAT mRNA accumulated most abundantly in root, and GalAT transcripts in all kinds of ramie tissues in turn revealed as follows： root 〉 leaf〉 bast 〉 or ≈ xylem.

Report on Screening Test of Dazhou Superior Ramie Germplasm Resources

[Objectives]To promote the development of the ramie industry in Dazhou City of Sichuan Province and provide a material basis for the breeding of new ramie varieties.[Methods]The Institute of Bast Fiber Crops of Dazhou Academy of Agricultural Sciences performed a screening test of excellent ramie germplasm resources from 2017 to 2019 to compare the growth,resistance,raw fiber yield and fiber fineness of the experimental materials.[Results]All the experimental materials showed strong growth potential,drought resistance and disease resistance.There were 8 kinds of resources with a fiber fineness greater than 2000 m/g,of which 3 kinds of resource materials had a fiber fineness greater than 2300 m/g;12 kinds of resources has the raw fiber yield exceeding the control and 10 kinds of resources had the raw fiber yield≥2000 kg/ha;3 kinds of resources met the requirements of the high-yield and high-quality indicators(fiber fineness exceeding 2000 m/g and raw fiber yield≥2000 kg/ha),they were BD0718,BD1614 and BYL2.[Conclusions]These high-quality ramie resources can provide a rich resource base for the breeding of new ramie varieties.

Prediction of Crude Protein in Ramie by Near-Infrared Spectroscopy(NIR)

[Objective] This study was conducted to evaluate the feasibility of deter- mining crude protein in ramie using near-infrared （NIR） spectrometer. [Method] Par- tial least square regression （PLSR） was performed to establish a calibration model based on 50 samples for predicting the crude protein content in ramie, and the model was validated with data in the validation set consisting of 10 samples. [Result] The correlation coefficient of the model was 0.98. There was a good correla- tion between the predicted values by the near-infrared prediction model and the measured values by chemical analysis, and the relative error was 3.54% on aver- age between the predicted and the measured values. [Conclusion] The results showed that it is feasible to determine crude protein content in ramie using NIR spectroscopy-based prediction model.

Structure of Ramie Treated by Liquid Ammonia

The morphology and supermolecular structures of the scoured/bleached ramie and scoured/bleached/liquid ammonia treated ramie were studied by means of scanning electron microscope, X-ray diffractometer, reverse gel permeation chromatography, etc. The results indic.ate that liquid ammonia treatment of the scoured/bleached ramie leads to a smoother surface, a lower crystalliuity, partial crystal transformation from cellulose Ⅰ to cellulose Ⅲ a decrease in the volume and cumulative surface area of the larger micropores and an increase in those of the smaller ones in the fiber.

Optimization for Microbial Degumming of Ramie with Bacillus subtilis DZ_(5) in Submerged Fermentation by Orthogonal Array Design and Response Surface Methodology

As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbial degumming process has many advantages.To obtain the optimal conditions for degumming ramie with Bacillus subtilis DZ_(5)(BS DZ_(5)),a combined statistical approach of orthogonal array design(OAD)and response surface methodology(RSM)was used.The influences of initial pH of the bacteria medium,culture temperature,shaking speed,degumming time and inoculum size on submerged fermentation degumming were evaluated by using fractional factorial design.The main factors in the analysis were culture temperature,shaking speed and initial pH.The residual gum mass fraction was used as the optimization index,and the optimal conditions for degumming were determined by central composite design and RSM.Thus with only a limited number of experiments,an optimal ramie microbial degumming condition was found as the culture temperature of 40℃,the initial pH in the culture medium of 8.5,the shaking speed of 205 r/min,the degumming time of 96 h and the inoculum size of 5%.After microbial degumming of ramie under the optimal conditions,there was only 10.6%residual gum by mass in the fiber.In addition,the effective degumming of BS DZ_(5)was also confirmed by a scanning electron microscope(SEM).

Mechanical Properties of Bio-Based Epoxy Composites Reinforced with Hybrid-Interlayer Ramie and Recycled Carbon Fibres

The growing environmental concerns have led to attention on bio-based composite materials, such as the natural fibres, recycled carbon fibres and bio-based resins. Herein, the bio-based epoxy composites were reinforced with ramie fibre (RF) and recycled carbon fibre (rCF) via inter-layer hybridisation. The dynamic mechanical analysis, tensile, flexural and impact properties characterisation were conducted to analyse the mechanical behaviour of the specimens. Also, the morphology of fractured surface after mechanical tests was studied under a scanning electron microscope. When the volume ratio between RF and rCF was varied from 100/0 to 0/100, the flexural and tensile strength of composites was significantly increased, while the impact strength was reduced. Thus the maximum values of flexural strength (182 MPa) and tensile strength (165 MPa) were observed for rCF reinforced composite, whilst impact strength of 24 kJ/m2 was found for RF reinforced composite. Furthermore, the values of storage and loss modulus were increased with the rCF incorporation due to a greater degree of restriction with the addition of rCF into the matrix. The hybridisation was able to combine the specific properties of RF and rCF and optimise the mechanical performance of composites. Therefore, the alternative low-cost green composites are prepared which can replace synthetic materials for semi-structural applications.

Preparation and Properties of Unmodified Ramie Fiber Reinforced Polypropylene Composites

Ramie fiber （RF） was used to reinforce the polypropylene （PP）. The composites were prepared with a melting hybrid technology. Tests had been performed on PP and composites with different RF contents （10 wt%, 20 wt%, and 30 wt%）. By using SEM, DSC, TGA, electronic universal testing machine, HDT-VICAT tester and coefficient of linear expansion tester, the effects of the RF loading were assessed on the basis of morphologies, mechanical and thermal properties as well as vicat softening temperature and CTE of the resulting composites. The results show that the thermal degradation temperature of the PP/RF composites becomes lower with higher fiber content. The crystallization rate of the PP matrix is accelerated by the unmodified RF. Because of the inferior interfacial bonding strength between RF and PP, the tensile strength of composites decreases by the presence of RF. And the RF used is relatively long compared with the diameter, the impact strength of the composites is improved by the unmodified RF. The vicat softening temperature of composites can be increased by about 5℃ in the presence of RF compared with PP. The CTE is reduced significantly in the presence of RF. Generally speaking, impact strength, crystallization rate, vicat softening temperature and CTE of PP/RF composites could be improved in the presence of RF. The tensile strength is decreased and thermal degradation temperature of composites becomes lower, but these should not affect most subsequent normal uses of the composites. As the unmodified RF is used directly, no hazardous waste is produced during the fabrication process, combined with the low price, so, a facile and economic preparation pathway is given by using unmodified natural fiber to reinforce polymer and composites with good performance obtained.

Relationship Between Physiological-biochemical Changes Under Drought Stress and Drought Resistance in Ramie

A study on the physiological-biochemical changes of different ramie(Boehmeria Jacq. ) varieties under drought stress was carried out, the results showed that relative water contents(RWC) decreased and RPP increased with the increase of drought stress. Compared with drought sensitive varieties, drought resistant varieties had higher RWC in leaves but lower RPP. Peroxidase activity of drought-resistance varieties changed from low to high whereas sensitive ones changed in opposite direction. Proline contents of drought sensitive varieties was higher than those of drought resistant ones under a certain degree drought stress. Proline accumulation in drought resistant varieties was faster than that in drought sensitive ones under serious drought stress.MDA contents in leaf was increased under drought stress. MDA contents increased slowly in resistant varieties while that increased rapidly in sensitive ones.

FUZZY DECISION-MAKING OF COMBING ROLLER COVERING FOR SPINNING PURE RAMIE NOIL ROTOR-SPUN YARNS

This paper deals with the types and specifications of combing roller covering for spinning pureramie noil rotor-spun yarns.A handling mode combining Fuzzy Decision-making and FuzzyCluster Analysis has been used for analyzing the experimental results.It is shown that,with regard to the specifications of the sawtooth clothing of the combing rol-ler,large working angle,large tooth pitch,fine tooth shape,short tooth height,smooth finish andgood wearability are of benefit to improving the spinning stability and the spun yarn properties.The pinned combing roller,however,regardless of its complicated process of production,is sug-gested to be preferred for spinning the pure ramie noil rotor-spun yarns.The handling mode used in this work is efficient in improving the reliability and objectivity ofthe conclusions and can be used for solving the similar problems.