将清洁级SD大鼠108只随机分为3组:对照组、脓毒症组以及脓毒症+益赛普组(治疗组),采用盲肠结扎穿孔术(CLP)建立脓毒症大鼠模型,分别于术后3、9、24、36、48 h和72 h 6个时相点从腹主动脉抽取血标本;采用酶联免疫分析法分别检测肿瘤坏死...将清洁级SD大鼠108只随机分为3组:对照组、脓毒症组以及脓毒症+益赛普组(治疗组),采用盲肠结扎穿孔术(CLP)建立脓毒症大鼠模型,分别于术后3、9、24、36、48 h和72 h 6个时相点从腹主动脉抽取血标本;采用酶联免疫分析法分别检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)的浓度。结果显示:1在脓毒症大鼠血清中细胞因子TNF-α、IL-1表达水平在术后3 h时为最大值,随着时间推移,两组浓度水平呈下降趋势,但仍高于对照组。2治疗组中TNF-α、IL-1的含量均显著高于对照组,但却低于脓毒症组,且组间差异有统计学意义(P<0.05)。表明rhTNFR:Fc可影响脓毒症大鼠早期炎症反应过程。展开更多
NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is i...NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.展开更多
Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. ...Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. Two hundred and forty male Wistar rats were randomized into a sham- operation group, an AMI group, and a recombinant human tumor necrosis factor receptor：Fc fusion protein（rhTNFR：Fc） group. Acute anterior wall myocardial infarction was produced in the AMI group by ligating the left anterior descending coronary artery （LAD）, and there was no ligation but operation in the sham-operation group. The rhTNFR：Fc group was treated with rhTNFR：Fc（10 mg/kg）, a TNF-a antagonist, 24 hours before LAD ligation. The spontaneous and induced programmed electrical stimulation ventricular arrhythmias were recorded at baseline and 10 minutes, 20 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 12 hours after ligation. At the same time the protein and mRNA expression levels of TNF-a among different groups were detected by histochemistry and real-time fluorescent quantitative PCR. Expression of TNF-a increased markedly from 10 minutes after infarction, peaked at 20-30 minutes, and returned to baseline gradually in the AMI group and rhTNFR：Fc group. The time- windows of spontaneous and induced ventricular arrhythmias were similar. Compared with the AMI group, the rhTNFR：Fc group showed a lesser expression of TNF-a protein and a lower incidence of ventricular arrhythmias （P〈0.05）. There was no obvious change in the sham-operation group. The expression of TNF-a induced by AMI could contribute to the onset of ventricular arrhythmias.展开更多
文摘将清洁级SD大鼠108只随机分为3组:对照组、脓毒症组以及脓毒症+益赛普组(治疗组),采用盲肠结扎穿孔术(CLP)建立脓毒症大鼠模型,分别于术后3、9、24、36、48 h和72 h 6个时相点从腹主动脉抽取血标本;采用酶联免疫分析法分别检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)的浓度。结果显示:1在脓毒症大鼠血清中细胞因子TNF-α、IL-1表达水平在术后3 h时为最大值,随着时间推移,两组浓度水平呈下降趋势,但仍高于对照组。2治疗组中TNF-α、IL-1的含量均显著高于对照组,但却低于脓毒症组,且组间差异有统计学意义(P<0.05)。表明rhTNFR:Fc可影响脓毒症大鼠早期炎症反应过程。
文摘NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.
文摘Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. Two hundred and forty male Wistar rats were randomized into a sham- operation group, an AMI group, and a recombinant human tumor necrosis factor receptor：Fc fusion protein（rhTNFR：Fc） group. Acute anterior wall myocardial infarction was produced in the AMI group by ligating the left anterior descending coronary artery （LAD）, and there was no ligation but operation in the sham-operation group. The rhTNFR：Fc group was treated with rhTNFR：Fc（10 mg/kg）, a TNF-a antagonist, 24 hours before LAD ligation. The spontaneous and induced programmed electrical stimulation ventricular arrhythmias were recorded at baseline and 10 minutes, 20 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 12 hours after ligation. At the same time the protein and mRNA expression levels of TNF-a among different groups were detected by histochemistry and real-time fluorescent quantitative PCR. Expression of TNF-a increased markedly from 10 minutes after infarction, peaked at 20-30 minutes, and returned to baseline gradually in the AMI group and rhTNFR：Fc group. The time- windows of spontaneous and induced ventricular arrhythmias were similar. Compared with the AMI group, the rhTNFR：Fc group showed a lesser expression of TNF-a protein and a lower incidence of ventricular arrhythmias （P〈0.05）. There was no obvious change in the sham-operation group. The expression of TNF-a induced by AMI could contribute to the onset of ventricular arrhythmias.
