As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor Os...As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.展开更多
目的探讨重组人对氧磷酶1K192亚型(r Hu PON1K192)对毒死蜱中毒导致的大鼠急性肝脏损伤的保护作用。方法将健康成年SD大鼠50只随机分为正常组(A组)、r Hu PON1K192对照组(B组)、毒死蜱染毒组(C组)、低剂量r Hu PON1K192预处理组(D组)、...目的探讨重组人对氧磷酶1K192亚型(r Hu PON1K192)对毒死蜱中毒导致的大鼠急性肝脏损伤的保护作用。方法将健康成年SD大鼠50只随机分为正常组(A组)、r Hu PON1K192对照组(B组)、毒死蜱染毒组(C组)、低剂量r Hu PON1K192预处理组(D组)、高剂量r Hu PON1K192预处理组(E组),每组10只。C、D、E组均给予毒死蜱灌胃,D组在灌毒30 min前予以r Hu PON1K192 4 500 U/kg尾静脉注射,E组予以9 000 U/kg r Hu PON1K192尾静脉注射,A组予以与毒死蜱同体积生理盐水灌胃,B组则单纯予以r Hu PON1K192尾静脉注射。8 h后取血,采用速率法检测谷丙转氨酶(ALT)及谷草转氨酶(AST)的活性,采用ELISA法检测苹果酸脱氢酶(MDH)及谷氨酸脱氢酶(GLDH)的活性;取肝脏组织行免疫组化检测缺氧诱导因子1α(HIF-1α)的表达,采用光镜、透射电镜检查超微结构的改变。比较各组之间相关指标的差异。结果比较A组与B组各项指标,差异均无统计学意义(P>0.05)。C组肝功能指标ALT、AST、GLDH及MDH较A组均有明显升高(P<0.01),HIF-1α呈重度表达,电镜与光镜下肝细胞膜、线粒体等出现明显损伤。D组及E组上述指标较A组轻度升高,E组改变程度较D组稍轻,2组各指标相比无统计学差异(P>0.05)。D组及E组上述指标改变较C组轻,差异均有统计学意义(P<0.05)。结论 r Hu PON1K192对毒死蜱中毒肝损伤具有保护作用。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32101763)China National Postdoctoral Program for Innovative Talents(Grant No.BX2021266)China Postdoctoral Science Foundation(Grant No.2021M692853).
文摘As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.
文摘目的探讨重组人对氧磷酶1K192亚型(r Hu PON1K192)对毒死蜱中毒导致的大鼠急性肝脏损伤的保护作用。方法将健康成年SD大鼠50只随机分为正常组(A组)、r Hu PON1K192对照组(B组)、毒死蜱染毒组(C组)、低剂量r Hu PON1K192预处理组(D组)、高剂量r Hu PON1K192预处理组(E组),每组10只。C、D、E组均给予毒死蜱灌胃,D组在灌毒30 min前予以r Hu PON1K192 4 500 U/kg尾静脉注射,E组予以9 000 U/kg r Hu PON1K192尾静脉注射,A组予以与毒死蜱同体积生理盐水灌胃,B组则单纯予以r Hu PON1K192尾静脉注射。8 h后取血,采用速率法检测谷丙转氨酶(ALT)及谷草转氨酶(AST)的活性,采用ELISA法检测苹果酸脱氢酶(MDH)及谷氨酸脱氢酶(GLDH)的活性;取肝脏组织行免疫组化检测缺氧诱导因子1α(HIF-1α)的表达,采用光镜、透射电镜检查超微结构的改变。比较各组之间相关指标的差异。结果比较A组与B组各项指标,差异均无统计学意义(P>0.05)。C组肝功能指标ALT、AST、GLDH及MDH较A组均有明显升高(P<0.01),HIF-1α呈重度表达,电镜与光镜下肝细胞膜、线粒体等出现明显损伤。D组及E组上述指标较A组轻度升高,E组改变程度较D组稍轻,2组各指标相比无统计学差异(P>0.05)。D组及E组上述指标改变较C组轻,差异均有统计学意义(P<0.05)。结论 r Hu PON1K192对毒死蜱中毒肝损伤具有保护作用。
