Influence of reduced-port laparoscopic surgery on perioperative indicators, postoperative recovery, and serum inflammation in patients with colorectal carcinoma

BACKGROUND Conventional five-port laparoscopic surgery,the current standard treatment for colorectal carcinoma(CRC),has many disadvantages.AIM To assess the influence of reduced-port laparoscopic surgery(RPLS)on perioperative indicators,postoperative recovery,and serum inflammation indexes in patients with CRC.METHODS The study included 115 patients with CRC admitted between December 2019 and May 2023,52 of whom underwent conventional five-port laparoscopic surgery(control group)and 63 of whom underwent RPLS(research group).Comparative analyses were performed on the following dimensions:Perioperative indicators[operation time(OT),incision length,intraoperative blood loss(IBL),and rate of conversion to laparotomy],postoperative recovery(first postoperative exhaust,bowel movement and oral food intake,and bowel sound recovery time),serum inflammation indexes[high-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)],postoperative complications(anastomotic leakage,incisional infection,bleeding,ileus),and therapeutic efficacy.RESULTS The two groups had comparable OTs and IBL volumes.However,the research group had a smaller incision length;lower rates of conversion to laparotomy and postoperative total complication;and shorter time of first postoperative exhaust,bowel movement,oral food intake,and bowel sound recovery;all of which were significant.Furthermore,hs-CRP,IL-6,and TNF-αlevels in the research group were significantly lower than the baseline and those of the control group,and the total effective rate was higher.CONCLUSION RPLS exhibited significant therapeutic efficacy in CRC,resulting in a shorter incision length and a lower conversion rate to laparotomy,while also promoting postoperative recovery,effectively inhibiting the inflammatory response,and reducing the risk of postoperative complications.

EFFECT OF INHALED NANO-TiO_2 ON LUNG AND SERUM BIOCHEMICAL INDEXES OF MICE

The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice（eight in each group）after exposure to TiO2 aerosols（1 500 mg/m3）in a sealed chamber.Another eight mice are exposed to indoor air to be served as a control group.Lung tissue and blood are collected after euthanizing the animals.The results show that lactate dehydrogenase（LDH）activity increases in all experimental groups.Alanine aminotransferase（ALT）activity and blood urea nitrogen（BUN）levels are increased in the group exposed to TiO2 aerosols for 28 d,and creatinine（Cr）levels are increased in 14 d and 28 d samples（P0.05）.No obvious changes are observed in other serum indexes.Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation,and many TiO2 particles are found in the interstitium of pulmonary alveoli.

Effects of Compound Herbal Medicine on Serum Biochemical Indexes and Egg Nutritional Quality in Laying Hens

[ Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutri- tional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 weeks were selected and randomly divided into five groups. After administration with different dosages of astragalus polysaccharides and berberine for 9 weeks, serum biochemical indexes were determined and nu- trient contents of egg were detected. [ ltesultl The contents of total protein, globulin, high-density lipoprotein cholesterol （HDL-C） and alkaline phosphatase （ALP） in all treatment groups were significantly （ P 〈 0.05 or 0.01 ） higher than those in the control group. The cholesterol contents of all treatment groups were significantly （ P 〈 0.05 or 0.01 ） lower than those of the control group. E Conclusion~ The optimal concentrations of as- tragalus polysacchaddes and berberine in basic diets are 200 and 30 mg/kg, respectively.

Effects of Fermentation Bed Padding on Intestinal Flora,Serum Biochemical Indexes and Immune Function of Growing-finishing Pigs

[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc × Landrace × Yorkshire)with the body weight of(30.5±0.8)kg were randomly divided into five groups,three replicates each group,six pigs each replicate.The padding of five groups were:50%rice husk+50%sawdust(control group),100%spent mushroom substrate of Pleurotus eryngii(100%spent mushroom substrate group),15%rice husk+15%sawdust+70%spent mushroom substrate(70%spent mushroom substrate group),25%rice husk+25%sawdust+50%spent mushroom substrate(50%spent mushroom substrate group),35%rice husk+35%sawdust+30%spent mushroom substrate(30%spent mushroom substrate group).The pre-trial lasted 7 d,and the formal trial lasted 90 d.[Result]Compared with the control group,the number of Escherichia col in 100%,70%and 50%spent mushroom substrate groups decreased by 35.4%,24.0%and 20.8%,respectively(P<0.05);the number of salmonella successively decreased by 29.4%,19.3%and 15.2%,respectively(P<0.05);the number of lactobacillus successively increased by 21.7%,15.9%and 12.2%,respectively(P<0.05);and the number of bifidobacteria successively increased by 20.2%,16.7%and 11.8%,respectively(P<0.05).Compared with the control group,the total serum protein content in 100%,70%,50%and 30%spent mushroom substrate groups increased by 30.6%,33.0%,28.0%and 18.4%,respectively(P<0.05);and the serum urea nitrogen content decreased by 32.4%,34.5%,33.9%and 22.0%,respectively(P<0.05).There was no significant difference in serum albumin,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)between treatment groups and control group.The IgA,IgG,IgM and IL-6 levels in serum of each treatment group were significantly different from those of the control group(P<0.05).There was no significant difference in IgA,IgG,IgM and IL-6 levels among 100%,70%and 50%spent mushroom substrate groups.[Conclusion]It improves the balance of intestinal microecological flora of growing-finishing pigs and enhances the immune performance of pigs by using spent mushroom substrate of P.eryngii as the fermentation bed padding.15%rice husk+15%sawdust+70%spent mushroom substrate is the optimal proportion of spent mushroom substrate for growing-finishing pigs.

EFFECTS OF INHALED TiO_2 NANOTUBES ON LUNG TISSUE AND SERUM BIOCHEMICAL INDEXES OF MICE

To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentration of which is 250mg / m 3 while another group of mice are exposed to room air only served as control.The blood , alveolar lavaged fluid and lungs of the mice are collected and examined after exposed for 7 , 14and 28d , respectively.The serum analysis shows that glucose ( Glu ) values with 7dexposure , alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities with 28dinhalation , and total bile acid ( TBA ) values with 7 , 28d as well as the creatine kinase ( CK ) levels with 28dexposure are significantly different from those of controls ( P< 0.05 ) .The pathological findings exhibit that more TiO 2 nanotubes are found in the interstitium of pulmonary alveoli with the experimental time prolonged.The results suggest that TiO 2 nanotubes do produce adverse responses to the lung and serum biochemical indexes of mice.Moreover , the responses become severer with the exposed-time prolonged.

Effects of Dietary Picria felterrae Lour Extracts on Serum Biochemical Indexes and Immune Function of Guangxi Partridge Chickens

[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.

Relationship Between Serum Leptin Concentration and Lipids & Body Mass Index in Patients With Chronic Congestive Heart Failure

Objectives To investigate the relationship of serum leptin concentration with the lipids and body mass index （BMI） in patients with chronic congestive heart failure（CHF）. Methods The serum concentration of leptin in 39 patients with CHF （14 in cardiac function class Ⅱ , 21 in class Ⅲ, 4 in classⅣ , NYHA） and in 46 patients with cardiac function class Ⅰ （NYHA） were assessed by radioimmunoassay. Results The serum concentration of leptin were 9.018±4.519 μg/l in CHF group （cardiac function class Ⅱ 11.492±5.649 μg/l, class Ⅲ 7.763±3.321 μg/l, class Ⅳ 6.100±2.657 μg/l）; 11.674± 6.911 μg/l in class Ⅰ group. The serum concentrations of leptin were significantly lower in CHF group, as compared with class Ⅰ group （P〈 0.05）. Moreover, the decrease of serum leptin concentration was significantly correlated with the decreased serum concentrations of total cholesterol, triglyceride, body mass index and left ventricular ejection fraction in CHF group, respectively （P 〈 0.05）. Conclusions The significance of the decrease in serum leptin in CHF patients needs further study.

Efficacy and anti-inflammatory analysis of glucocorticoid,antihistamine and leukotriene receptor antagonist in the treatment of allergic rhinitis

BACKGROUND There are many adverse reactions in the treatment of allergic rhinitis(AR)mainly with conventional drugs.Leukotriene receptor antagonists,glucocorticoids and nasal antihistamines can all be used as first-line drugs for AR,but the clinical effects of the three drugs are not clear.AIM To examine the impact of glucocorticoids,antihistamines,and leukotriene receptor antagonists on individuals diagnosed with AR,specifically focusing on their influence on serum inflammatory indexes.METHODS The present retrospective study focused on the clinical data of 80 patients diagnosed and treated for AR at our hospital between May 2019 and May 2021.The participants were categorized into the control group and the observation group.The control group received leukotriene receptor antagonists,while the observation group was administered glucocorticoids and antihistamines.Conducted an observation and comparison of the symptoms,physical sign scores,adverse reactions,and effects on serum inflammatory indexes in two distinct groups of patients,both before and after treatment.RESULTS Subsequent to treatment,the nasal itching score,sneeze score,runny nose score,nasal congestion score,and physical signs score exhibited notable discrepancies(P<0.05),with the observation group demonstrating superior outcomes compared to the control group(P<0.05).The interleukin(IL)-6,IL-10,tumor necrosis factor-alpha,Soluble Intercellular Adhesion Molecule-1,Leukotriene D4 after treatment were significantly different and the observation group It is better than the control group,which is statistically significant(P<0.05).Following the intervention,the incidence of adverse reactions in the observation group,including symptoms such as nasal dryness,discomfort in the throat,bitter taste in the mouth,and minor erosion of the nasal mucosa,was found to be 7.5%.This rate was significantly lower compared to the control group,which reported an incidence of 27.5%.The difference between the two groups was statistically significant(P<0.05).CONCLUSION Glucocorticoids and antihistamines have obvious therapeutic effects,reduce serum inflammatory index levels,relieve symptoms and signs of patients,and promote patients'recovery,which can provide a reference for clinical treatment of AR.

Effects of Chinese Medicine Additive on Promoting Production of Meat Hybrid Chicken and Its Action Mechanism

[ Objective] The aim was to study the effects of Chinese medicine additive HMS on promoting the production of meat hybrid chicken and its action mechanism, so as to provide a scientific basis for the promotion effects of Chinese medicine additive on production. [ Method] Meat hybrid chickens, 7 day-old with similar weight, were randomly divided into four groups. And Chinese medicine feed additive HMS was added to the diet of each group at different proportions. All the chickens were weighed before the test as well as at the end of the 2nd, 3rd, 4^th and 5^th week. The feed consumption was also recorded during the test. Before slaughter, serum samples were collected from the randomly selected chickens for the deter- mination of serum biochemical indexes. Their bursae of Fabdcius and spleens were collected and weighed in order to determine the indexes, of im- mune organs. [ Result] HMS significantly reduced the feed conversion ratio （ P 〈 0.05 ）, very significantly reduced the feed consumptioh （ P 〈 0.01 ）, very significantly improve the content of serum high-density lipoprotein （ P 〈 0.01 ）, significantly increased the activity of serum alkaline phos- phatase （ P 〈 0.05）, very significantly reduced the content of serum urea nitrogen and uric acid （ P 〈 0.01 ）, and significantly reduced the level of serum cholesterol （ P 〈0.05）. There were no significant difference between the test groups and the control group （ P 〉 0.05） in the increase of im- mune organ indexes, but the index of immune organs has increasing trend. [ Conclusion] HMS reduces feed consumption and feed conversion rati- o, improves the feed utilization, and has a role in promoting production. The possible mechanism may be HMS improving the activity of serum alka- line phosphatase and the level of serum high-density lipoprotein, as well as reducing the content of serum cholesterol, urea nitrogen and uric acid in meat hybrid chickens.

Effects of weaning age on growth, nutrient digestibility and metabolism,and serum parameters in Hu lambs

This study was conducted to investigate the effect of weaning age on growth performance, nutrient digestion and metabolism, and serological indicators, and to obtain an optimal weaning age in Hu lambs.Forty-eight newborn Hu lambs(birth weight, 2.53 ± 0.14 kg) were randomly divided into 4 groups. The lambs in control group(ER) suckled their dams. The lambs in other three experimental groups were weaned on milk replacer at 10.20, and 30 days of age(EW10, EW20, and EW30 groups),respectively. The results were as follows: 1) lambs in EW10 and EW30 groups had a lower(P < 0.05) ADG than those in ER group within 10 days post-weaning; the weaned lambs began to show a higher(P < 0.05) ADG than those in ER group after 20 days post-weaning. 2) EW10 and EW20 groups had a higher(P < 0.05) creep feed intake than EW30 and ER groups from 15 to 60 days of age. 3) The apparent digestibility of dry matter, organic matter, gross energy, nitrogen, ether extract and phosphorus, and the deposition of nitrogen and phosphorus did not differ(P > 0.05) among groups; however, the apparent digestibility and deposition of calcium in early weaned lambs were lower(P < 0.05) than those in ewe-reared lambs. 4)The albumin content in EW30 group was lower(P < 0.05) than that in ER group; the globulin content in EW30 group was higher(P < 0.05) than that in other groups; the content of serum insulin-like growth factor-1 in weaned lambs tended to increase compared with lambs in ER group. Finally, the growth rate of lambs decreased within 10 days post-weaning, but early weaning boosted creep feed intake, leading to better growth and health later in life. The Hu lambs can be weaned on milk replacer and creep feed at 10 days of age.

A correlative study of iron metabolism based on q-Dixon MRI in benign prostatic hyperplasia and prostate cancer

Clinical staging,Gleason score,and prostate-specific antigen(PSA)have been accepted as factors for evaluating the prognosis of prostate cancer(PCa).With the in-depth study of iron metabolism and the development of multiparametric magnetic resonance imaging technology,we used q-Dixon magnetic resonance imaging(MRI)to measure the iron content of the PCa patients'lesions,and used enzyme-linked immunosorbent assay(ELISA)to measure the iron metabolism indicators in the patients'serum samples,combined with the patients'postoperative clinical data for analysis.We found that the serum indexes were correlated with the T2 star values,International Society of Urological Pathology(ISUP)grade,and pathological classification in PCa patients(all P<O.001)but not in benign prostatic hyperplasia(BPH)patients(all P>O.05).The utilization of q-Dixon-based MRI and serum indexes allows the noninvasive measurement of iron content in prostate lesions and the assessment of differential iron metabolism between PCa and BPH,which may be helpful for evaluating the prognosis of PCa.