Effects of Seed Dressing with Microbial Inoculum on Nutrient Composition and Biological Yield of Silage Corn

[Objectives]This study was conducted to understand the effects of microbial inocula on the biological yield and nutritional components of corn.[Methods]Silage corn varieties suitable for planting in Hebei Province were selected,and set with an experimental group and a control check group each.Meanwhile,at the late stage of milk ripening of silage corn growth cycle,the nutritional components and indexes of silage corn were determined and analyzed by a near-infrared analyzer and the NIRS technique.Meanwhile,the biological yield of silage corn was determined at the maturation stage of its growth period,aiming at comprehensively evaluating the regulation effects of the microbial inoculum tested on silage corn from the aspects of basic nutritional components,mineral element contents,energy and related indexes of corn,combined with agronomic characters and biological yield.[Results]The microbial inoculum improved the biological yield and nutritional indexes of silage corn,and had a positive regulation effect on the growth of silage corn.[Conclusions]The results of this study provide a theoretical basis for popularizing the planting methods and cultivation methods of silage corn with high yield and high quality.

Screening and Genetic Characteristics of Breeding Materials of Silage Corn

[Objective] To analyze the effects of major agronomic traits on yield in maize inbred lines. [Method] Incomplete diallel cross between three single-cross hybrids and 19 parent materials of com silage were performed, and the agronomic traits of the obtained 57 cross combinations were identified. [ Result] The regression equations between biomass and four important indicators （grain yield, rows per ear, plant height and straw yield） affecting biomass of com silage were established. The broad heritability of straw yield, plant height, rows par ear and ear length was higher than 50%. Genetic effects had a greater impact, while environmental factors had a smaller impact. Thus, it had great potential to improve these traits by genetic breeding. In addition, the broad heritability of biomass and grain yield was lower than 50%. [ Conclusion] Biorness and grain yield have greater interaction effects on environment, and the environmental impact should be considered for selection.

Effects of Whole-plant Corn Silage and Whole-plant Wheat Silage on Growth Performance and Economic Benefits of Beef Cattle

[Objectives]This study was conducted to study the effects of whole-plant corn silage and whole-plant wheat silage on growth performance and economic benefits of beef cattle.[Methods]Thirty Simmental crossbred cattle were selected and randomly divided based on their weight into two groups,15 cows in each group,with an average weight of 398 kg.Each group was fed the same concentrated feed,and the whole-plant corn silage and wheat silage were fed as roughage feed,respectively.The experimental period was 90 d.[Results](1)There was no significant difference in the initial weight of beef cattle between test group 1 and test group 2(P>0.05).Compared with group 2,group 1 had a higher final weight,average daily gain,and dry matter intake of beef cattle,without significant differences(P>0.05).Group 1 was 0.58%,1.99%,and 1.43%higher than group 2,respectively.The feed-to-gain ratio of test group 1 decreased by 0.54%compared with test group 2(P>0.05),with no significant difference therebetween.(2)The economic benefit of group 1 was 11.45 yuan/(day·capita),while the economic benefit of group 2 was 2.41 yuan/(day·capita),and group 1 was 9.04 yuan/(day·capita)higher than group 2.[Conclusions]Whole-plant corn silage for feeding beef cattle has the advantages of good weight gain and low breeding cost,and can be popularized in beef cattle fattening.

Controlling threshold in soil salinity when planting spring wheat and sequential cropping silage corn in Northern Xinjiang using drip irrigation

Xinjiang Region of China is one of the most typical and representative arid areas worldwide,along with severe soil salinization issue.Planting spring wheat and sequential cropping silage corn in Northern Xinjiang using drip irrigation has become an effective way to relieve soil salinity stress,which improves the simple agricultural structure in the past and ensures food security in this area.However,neither the effects of different soil salinities on the growth and yield of spring wheat and silage corn,nor their desalination effect correspondingly was clear until now.Therefore,a pot experiment was conducted at Shihezi,Xinjiang from March to June 2015.The study aimed to establish the quantitative correlations between the parameters mentioned above and came up with the appropriate soil salinity threshold in Northern Xinjiang area.The results confirmed that the soils in all treatments were desalinated after the whole growth period,and the decreasing rates varied within 18.89%-44.08%and 11.06%-30.83%for two plants,which showed linear and quadratic correlations with initial soil salinity,respectively(R^(2)>0.92^(**),p<0.05).Meanwhile,higher soil salinity would inhibit crop growth and yield,and the initial soil salinity also represented the negative quadratic correlations with growth parameters(R^(2)>0.92^(**),p<0.01).The inhibition effect was enhanced with larger initial soil salinity.After the comprehensive consideration of soil salinity variation,crop growth and yield,the initial soil salinity was recommended under 8.91 g/kg and 5.54 g/kg to plant spring wheat and sequential cropping silage corn in Northern Xinjiang using drip irrigation.

Comparison of forage yield,silage fermentative quality,anthocyanin stability,antioxidant activity,and in vitro rumen fermentation of anthocyanin-rich purple corn(Zea mays L.) stover and sticky corn stover

The objective of this study was to observe the forage yield, silage fermentative quality, anthocyanin stability, and antioxidant activity during the storage period and in vitro rumen fermentation of anthocyanin-rich purple corn （Zea mays L.） stover （PS） and sticky corn stover （SS）. Forage yield of corn stover was weighed and ensiled with two treatments： （1） hybrid sticky waxy corn stover （control）, and （2） hybrid purple waxy corn stover （treatment）. Samples were stored in mini-silos for periods of 0, 7, 14, 21,42, 63, 84, and 105 d. The results showed that PS had significantly higher （P〈0.05） yields of dry matter （DM）, organic matter （OM）, gross energy （GE）, crude protein （CP）, neutral detergent fiber （NDF）, acid detergent fiber （ADF）, and total anthocyanins than that of the SS. Anthocyanin-rich purple corn stover silage （PSS） showed higher （P〈0.05） levels of DM and CP relative to the sticky corn stover silage （SSS）. Although anthocyanin-rich PSS displayed a lower （P〈0.05） level of pelargonidin-3-glucoside （P3G）, it had higher （P〈0.05） levels of peonidin （Peo） and pelargonidin （Pel） compared to the control. Delphinidin （Del） and malvidin （Mal） were not detected in SSS during the ensilage period; in PSS, Del was no longer detected after 7 d of ensilage. Specifically, total anthocyanins in anthocyanin-rich PSS decreased rapidly （P〈0.05） prior to 7 d of ensilage, and then remained at relatively stable （P〉0.05） constants. Compared to the anthocyanin-rich PSS, SSS displayed significantly higher （P〈0.05） pH value and ammonia nitrogen （NH3-N） content. Propionic acid （PA） at 0 d and butyric acid （BA） during the entire study period were not detected, whereas anthocyanin-rich PSS showed a higher （P〈0.05） level of lactic acid （LA） than that of the SSS. Compared with the SSS extract, anthocyanin-rich PSS extract showed a higher （P〈0.05） level of 2,2-diphenyl-1-picryihydrazyl （DPPH） scavenging activity and displayed a lower （P〈0.05） half maximal inhibitory concentration （IC50） value. Moreover, anthocyanin-rich PSS reduced （P〈0.05） gas production （GP）, and displayed lower levels of immediately soluble fraction and ratio of acetic acid （AA） to PA at 12 h, but the other parameters were unaffected （P〉0.05） relative to the control. Taken together, the results indicated that： （1） anthocyanins could be stable in silage; （2） anthocyanin-rich PSS showed better silage fermentative quality and stronger antioxidant activity; and （3） anthocyanin-rich PSS had no negative effect on rumen fermentation parameters.

Review of Factors Affecting the Yield and Quality of Corn Silage

Corn silage is the most popular and important source of energy and fber for ruminants. Much research focused on the chopping length, additives, sealing system, harmful bacteria and their toxins, and so forth, has been done on corn silage both at home in China and abroad. Some suggestions were presented in this review： （1） Corn silage modulation： Whole-plant corn harvested at the milk-ripe stage with a dry matter （DM） content of 30%~40% was selected as silage material and treated by rubbing and cutting. In the process of silage, commercial Lactobacillus buchneri was inoculated, exogenous protease added appropriately, cellulose enzyme added depending on the phenological period, the silage container and the species of the animals, and coextruded polyethylene？polyamide oxygen barrier flm （OB） selected as the seal material. （2） Strengthening management： Aerobic exposure should be avoided at the storage stage and reduced during the feeding phase. The proportion of corn silage was increased depending on the type of animal and their management. （3） Suggestions for further research： Further studies would be needed for the effects of liquid and solid wastes on soil, water and vegetation, and the treatment of these wastes.

Carbon Monoxide Emissions from Corn Silage

Carbon monoxide (CO) emissions from corn silage were observed using Fourier Transform Infrared (FTIR) spectroscopy and laser spectroscopy. In the first experiment, corn silage was produced using laboratory bucket silos. Air samples were collected from the bucket silos during the first week of ensiling and analyzed using a low-resolution Bruker FTIR spectrometer coupled with a long optical path length White Cell. The CO concentration in the bucket silo gas, derived from the FTIR spectra using the LINEFIT program, was as high as 48.0 ppm. In the second experiment, air samples were collected through a flux chamber from an Ag-Bag silage pile on a commercial dairy that was opened several months after ensiling. The Ag-Bag air samples were analyzed using a high-resolution Bruker FTIR spectrometer, and CO concentrations were retrieved to be 6.83 ppm, corresponding to an area emission rate of 33.7 mg/(hour ·m2). An LGR N2O/CO gas analyzer based on infrared laser spectroscopy was also used to measure the CO concentrations from the same flux chamber. Elevated CO concentrations were observed from these silage sources. The present study revealed that CO was emitted by corn silage during different phases of ensiling. Annual CO emissions from the corn silage were estimated to be much lower than those from the well-known emission categories in San Joaquin Valley (SJV) and California, but comparable to those from food and agricultural sources in the SJV. It is also confirmed that FTIR spectroscopy is a viable method for measuring CO concentrations in complex gas mixtures, such as silage gas.

Carcass Traits, Meat Yield and Primal Meat Cuts from Arsi, Harar, Ogaden and F1 Jersey*Horro Crossbred Bulls Fed Corn Silage Based Similar Finishing Diet

This study was conducted with the objective to determine carcass traits, meat yield, and primal meat cuts of Arsi, Harar, Jersey*Horro crossbred, and Ogaden cattle breeds at Haramaya University, Ethiopia. A total of 12 bulls of four cattle breeds (3 Arsi, 3 Harar, 3 F1 Jersey*Horro crossbred and 3 Ogaden) with almost similar ages were randomly assigned to four treatments in a completely randomised design (CRD). Data on carcass traits, meat yield, and primal meat cuts were analyzed using the General Linear Model (GLM) of the Statistical Analysis Software (SAS) 9.4 version. The overall averages of live body weight, hot carcass weight, chilled carcass weight, dressing percentages based on hot carcass weight, and rib eye area of experimental cattle breeds were 215.58 kg, 102.93 kg, 99.56 kg, 47.61%, and 8.13 inch2, respectively. The hot carcass weight and chilled carcass weight of the Ogaden (136.57;133.30 kg, resp.) breed were higher (p < 0.01) compared to other experimental cattle breeds. Dressing percentages based on hot carcass weight were higher (p < 0.05) for the Ogaden (49.61%) and Arsi (49.82%) cattle breeds compared to Harar and Jersey*Horro crossbred (45.73%, 45.27%, resp.) cattle breeds. The average meat yield and proportion of meat yield of cattle breeds were 77.52 kg and 77.46%, respectively. With a linear regression coefficient of prediction (R2) ranging from 52.26% to 93.58%, primal meat cuts significantly (p dicted meat yield. In conclusion, the breed of cattle had a significant (p 0.05) influence on live body weight, hot and chilled carcass weight, dressing percentage, rib eye area, subcutaneous fat thickness, meat yield, and the weights of most primal meat cuts. The Ogaden cattle breed had a higher and better meat yield, carcass traits, and most primal meat cuts compared to other experimental cattle breeds. Furthermore, the inclusion of corn silage in the diet of fattening bulls improved the carcass and meat yield. Therefore, the performance of Ogaden cattle compared to other and previous studies suggests the possibility of using this breed for export purposes in addition to Borana and Harar cattle breeds in the future.

Detecting Nitrous Oxide in Complex Mixtures Using FTIR Spectroscopy: Silage Gas

Nitrous oxide (N2O) is a greenhouse gas with about 300 times the global warming potential (GWP) of carbon dioxide (CO2). It is emitted from a wide range of sources and is responsible for about 6% of anthropogenic US greenhouse gas emissions. Analytical techniques are needed that can measure concentrations of N2O rapidly and inexpensively in sources that are also emitting other compounds that may interfere with the analytical process. In this work, we demonstrate the use of Fourier Transform Infrared (FTIR) spectroscopy to analyze N2O in the complex mixture of gases produced during the early phase of the silage making process. Silage gas samples were collected into Tedlar bags from the bucket silos during the first week of corn ensiling. A bag of the silage gas was analyzed using a Bruker FTIR spectrometer coupled with a long optical path length White Cell. First, N2O infrared absorption bands were identified in the FTIR spectra of the silage gas by comparing them to both standard N2O gas and simulated infrared spectra which confirmed that N2O was present in the silage gas. Then, N2O concentration in the silage gas was derived from the FTIR spectra using LINEFIT program. It was demonstrated that FTIR spectroscopy is a viable method for measuring N2O concentrations in the silage gas.

Determination of water content in corn stover silage using near-infrared spectroscopy

The aim of this study was to evaluate the feasibility of utilizing near-infrared spectroscopy to determine the water content of corn stover silage across a wide range.The water contents of 208 samples were measured,and their corresponding near-infrared spectra were simultaneously collected.The effects of different preprocessing methods,such as derivation,standard normal variety(SNV),multiplicative scatter correction(MSC),and non-preprocessing methods for the obtained near-infrared spectra on the performance of calibration models were compared.The calibration models were established by modified partial least squares(MPLS)regression.The results showed that the calibration model developed from the successive preprocessing of MSC and first-order derivation(1-D)achieved the optimal performance.The correlation coefficients of the calibration and validation subset were 0.974 and 0.949,respectively,and the standard errors of the calibration and cross validation were 4.249% and 4.256%,respectively.External validation was performed on 60 samples.The correlation coefficient between the measured and predicted values of the calibration model was 0.973 and the prediction model’s relative percent deviation was 4.317.This indicated that the mathematical model of near-infrared spectroscopy predicted the water content in corn stover silage with high accuracy.The study showed that the near-infrared spectroscopy technology can be used for rapid and non-destructive testing across a wide range of water contents in the corn stover silage.

Evaluation of alfalfa inter-seeding effect on bahiagrass baleage fermentation and lactating Holstein performance

Background：Previous research indicates that bahiagrass may be successfully conserved as baleage,but nutritive value is typically low for lactating dairy cows.The purpose of this study was to determine the effect of adding modest amounts of alfalfa forage（22%）,achieved by inter-seeding alfalfa into an existing bahiagrass pasture,on baleage nutritive value and lactation performance of Holstein cows.Forage treatments employed were monoculture bahiagrass baleage（MBB;negative control）,bahiagrass-alfalfa mixture baleage（BAB） and conventional corn silage（CCS;positive control）.Thirty six mid lactation Holstein cows[34.8 ±5.8 kg 3.5%fat-corrected milk and 112 ± 19 d in milk（DIM）]were stratified according to milk yield and DIM and assigned randomly to 1 of 3 forage treatments.Cows were trained to Calan feeding gates and were offered a common CCS-based TMR in a 10-d covariance period followed by a 42-d treatment feeding period.Results：The BAB contained more protein and less NDF than MBB（12.6 vs 10.3%CP and 71.8 vs 76.6%NDF）.Diet DMI was similar for MBB and BAB（19.5 vs 21.6 kg/hd/d）,but cows consumed more of the CCS diet（25.5 kg/hd/d）than either baleage-based diet.Cows offered BAB tended to produce more milk than cows offered MBB based TMR（28.4 vs 26.1 kg/hd/d）,but both baleage diets generated less milk than CCS-based diets（33.1 kg/hd/d）.Milk cumpusition was similar across diets except for milk protein concentrations which were higher tor CCS than either MBB or BAB diets;however,milk urea nitrogen（MUN） was lowest for cows fed CCS diets.Cow BW gain was higher for BAB than MBB implying that a portion of the higher energy contributed by the alfalfa was being used to replenish weight on these mid lactation cows.Conclusions：Data from this study indicate that alfalfa inter-seeded in bahiagrass sod that produces BAB with as little as 22%alfalfa may improve nutritive value compared to monoculture bahiagrass baleage and marginally improve lactation performance of Holstein cows.However,the CCS diet was vastly superior to either MBB or BAB-based diets for milk production.