Variation Characteristics of Root Traits of Different Alfalfa Cultivars under Saline-Alkaline Stress and their Relationship with Soil Environmental Factors

Soil salinization is the main factor that threatens the growth and development of plants and limits the increase of yield.It is of great significance to study the key soil environmental factors affecting plant root traits to reveal the adaptation strategies of plants to saline-alkaline-stressed soil environments.In this study,the root biomass,root morphological parameters and root mineral nutrient content of two alfalfa cultivars with different sensitivities to alkaline stress were analyzed with black soil as the control group and the mixed saline-alkaline soil with a ratio of 7:3 between black soil and saline-alkaline soil as the saline-alkaline treatment group.At the same time,the correlation analysis of soil salinity indexes,soil nutrient indexes and the activities of key enzymes involved in soil carbon,nitrogen and phosphorus cycles was carried out.The results showed that compared with the control group,the pH,EC,and urease(URE)of the soil surrounding the roots of two alfalfa cultivars were significantly increased,while soil total nitrogen(TN),total phosphorus(TP),organic carbon(SOC),andα-glucosidase activity(AGC)were significantly decreased under saline-alkaline stress.There was no significant difference in root biomass and root morphological parameters of saline-alkaline tolerant cultivar GN under saline-alkaline stress.The number of root tips(RT),root surface area(RS)and root volume(RV)of AG were reduced by 61.16%,44.54%,and 45.31%,respectively,compared with control group.The ratios of K^(+)/Na^(+),Ca^(2+)/Na^(+)and Mg^(2+)/Na^(+)of GN were significantly higher than those of AG(p<0.05).The root fresh weight(RFW)and dry weight(RDW),root length(RL),RV and RT of alfalfa were positively regulated by soil SOC and TN,but negatively regulated by soil pH,EC,and URE(p<0.01).Root Ca^(2+)/Na+ratio was significantly positively correlated with soil TN,TP and SOC(p<0.01).The absorption of Mg and Ca ions in roots is significantly negatively regulated by soilβ-glucosidase activity(BGC)and acid phosphatase activity(APC)(p<0.05).This study improved knowledge of the relationship between root traits and soil environmental factors and offered a theoretical framework for elucidating how plant roots adapt to saline-alkaline stressed soil environments.

Rhizosphere Soil Fungal Diversity and Soil Physicochemical Properties of Different Vegetations in Tundra of Changbai Mountain

By studying the diversity and community structure of rhizosphere soil fungi of different plants in the tundra on the northern slope of Changbai Mountain, it provides theoretical support for the restoration of environmental degradation and in-depth study of fungal diversity in the tundra of Changbai Mountain. High-throughput sequencing technology was used to determine the ITS1 region of fungal amplicons, so as to analyze the diversity of fungal communities in the rhizosphere soil of six plants in the tundra of Changbai Mountain, and to analyze the correlation between the environment and the diversity and richness of fungal communities in combination with relevant soil physical and chemical factors. The diversity and richness of fungal community in the rhizosphere soil of six plants in Changbai Mountain tundra were different. The Simpson and Shannon indexes of Saxifraga stolonifera Curt were the highest, and the richness of fungal community in Dryas octopetala was the highest. The analysis of fungal community composition showed that the fungal colonies in plant rhizosphere soil samples mainly belonged to Ascomycota and Basidiomycota, which were the main dominant phyla. Mortierella, Fusarium and Sordariomycetes are common fungal genera in the rhizosphere soil of six plants, but their abundances are different among different plants. Water content was negatively correlated with fungal diversity, and TP was positively correlated with fungal community diversity. There were some differences in the composition and diversity of rhizosphere soil fungal communities of six plants in Changbai Mountain tundra. Ascomycota and Basidiomycota were the main soil fungal phyla in the rhizosphere of six plants in Changbai Mountain tundra. The results could provide theoretical guidance for ecological protection of Changbai Mountain tundra.

Graphene oxide influences bacterial community and soil environments of Cd-polluted Haplic Cambisols in Northeast China

Graphene oxide(GO),a carbon nanomaterial that is widely used in the environment and other industries,may pose potential risks to ecosystems,especially the soil ecosystem.Some soils in Northeast China are frequently polluted with cadmium(Cd) metal.However,there is no study on the influence of GO on the Cd-contaminated soil microbial community and soil chemical properties.In this study,Cd(100 mg kg^(-1))-polluted soils were treated with different concentrations of GO(0,25,50,150,250,and 500 mg L^(-1),expressed as T1,T2,T3,T4,T5,and T6,respectively) for 40 days.The treatment without Cd pollution and GO served as the control(CK).Then,we investigated the influence of the GO concentrations on the bacterial community and chemical properties of Cd-polluted Haplic Cambisols,the zonal soil in Northeast China.After GO addition,the richness and diversity indexes of the bacterial community in Cd-contaminated Haplic Cambisols initially increased by 0.05-33.92% at 25 mg L^(-1),then decreased by0.07-2.37% at 50 mg L^(-1),and then increased by 0.01-24.37%within 500 mg L^(-1) again.The species and abundance of bacteria varied with GO concentration,and GO significantly increased bacterial growth at 25 and 250 mg L^(-1).GO treatments influenced the bacterial community structure,and the order of similarity of the bacterial community structure was as follows:T4=T5> T1=T6> T2> T3> CK.Proteobacteria and Acidobacteria were the dominant bacteria,accounting for 36.0% and 26.2%,respectively,of soil bacteria.Different GO treatments also significantly affected the metabolic function of bacteria and further influenced the diversity of the bacterial community structure by affecting several key soil chemical properties:soil pH,organic matter and available potassium,phosphorus,and cadmium.Our results provide a theoretical basis for scientific and comprehensive evaluation of the environmental impacts of GO on the zonal forest soils of Northeast China.

Impacts of changed litter inputs on soil CO_2 efflux in three forest types in central south China

Climate change is expected to cause the alteration of litter production in forests, which may result in substantial changes in soil CO2 effiux （FCO2） process as litter represents a major pathway of carbon from vegetation to the soils. In this study, we conducted an aboveground litter manipulation experiment to examine the influence of litter addition and exclusion on soil FCO2 in Camphor tree, Masson pine, and mixed Camphor tree and Masson pine forests in central south China. Litter input manipulation included three treatments： nonqitter input （litter exclusion）, double litter input （litter addition）, and natural litter input （control）. On average, litter exclusion significantly reduced soil FCO2 rate by approximately 39%, 24% and 22% in Camphor tree forests, the Mixed forests, and Masson pine forests, respectively. On a yearly basis, double litter addition significantly increased soil CO2 by 12% in the Mixed forests （P=0.02） but not in both Camphor tree and Masson pine forests （P〉0.05）, when compared with their corre- sponding control treatments. However, litter addition increased soil FCO2 rates in the months of June-August in Camphor tree and Masson pine forests, coinciding with high soil temperature of summer conditions. Litter exclusion reduced soil FCO2 more than litter addition increased it in the study sites. Responses of soil respiration to litter input treatments varied with forest types. Litter input treatments did not alter the seasonal patterns of soil temperature and soil water content. Our results indicated that changes in aboveground litter as a result of global climate change and/or forest management have a great potential to alter soil respiration and soil carbon balance in forest ecosystems.

Adsorption behavior of antibiotic in soil environment： a critical review

Antibiotics are used widely in human and veterinary medicine, and are ubiquitous in environment matrices worldwide. Due to their consumption, excretion, and persistence, antibiotics are disseminated mostly via direct and indirect emissions such as excrements, sewage irrigation, and sludge compost and enter the soil and impact negatively the natural ecosystem of soil. Most antibiotics are amphiphilic or amphoteric and ionize. A non-polar core combined with polar functional moieties makes up numerous antibiotic molecules. Because of various molecule structures, physicochemical properties vary widely among antibiotic compounds. Sorption is an important process for the environment behaviors and fate of antibiotics in soil environment. The adsorption process has decisive role for the environmental behaviors and the ultimate fates of antibiotics in soil. Multiply physicochem- ical properties of antibiotics induce the large variations of their adsorption behaviors. In addition, factors of soil environment such as the pH, ionic strength, metal ions, and organic matter content also strongly impact the adsorption processes of antibiotics. Review about adsorption of antibiotics on soil can provide a fresh insight into understanding the antibiotic-soil interactions. Therefore, literatures about the adsorption mechanisms of antibiotics in soil environment and the effects of environment factors on adsorption behaviors of antibiotics in soil are reviewed and discussed systematically in this review.