Bioremediation of Textile Azo Dyes Amido Black 10B, Reactive Black 5, Reactive Blue 160 by Lentinus squarrosulus AF5 and Assessment of Toxicity of the Degraded Metabolites

Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, 1H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar (Cyamopsis tetragonoloba) and wheat (Triticum aestivum) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.

Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by <i>Lentinus squarrosulus</i>

The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81;sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Lentinus squarrosulus Mont. is an emerging tropical white rot basidiomycete, with nutritional and medicinal benefits. Low levels of commercial cultivation of the mushrooms limit their availability for use as food and medicine. Mycelia from submerged fermentation are a suitable alternative to the mushroom from L. squarrosulus. Three strains, 340, 339 and 218, were studied to determine optimum growth conditions for mycelia mass and crude exo-polysaccharides (CEPS) production. The experiments were conducted in a completely randomized design (CRD) with a factorial structure. Nutrients involving 8 carbon and 8 nitrogen sources were screened, and concentrations of the best sources were optimized. Optimized nutrients, interaction between strains and other parameters such as agitation and medium volume were investigated to obtain optimum fermentation conditions for biomass and CEPS production. Biomass yield varied among strains depending on carbon or nitrogen nutrient sources. Starch and yeast extract at 30 and 25 g/L were identified as the most important nutrients in mycelia and CEPS production. Nutrient optimization resulted in a 3-fold increase in mycelia mass: 12.8, 10.0 and 15.3 g/L in strains 340, 339 and 218 respectively. There was a significant interaction between strain, agitation, and volume (p p = 0.02). Static conditions favored more polysaccharide production. Optimized fermentation conditions resulted in very high increase in biomass: 238.1, 266.9 and 185.0 g/L in strains 340, 339 and 218 respectively. Results obtained could be useful in modeling fermentation systems for large-scale production of mycelia mass, CEPS and other bio-products from L. squarrosulus.

Antioxidant Properties and Glucan Compositions of Various Crude Extract from <i>Lentinus squarrosulus</i>Mycelial Culture

Various crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan and carbohydrate compound analysis. Crude extract from ethanol, cold and hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, total flavonoid compound, total carbohydrate content and total glucan compositions. The antioxidant activity of the crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth was determined by 2,2’ diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene linoleic acid assay. Crude extracted from culture broth EPS EE recorded the highest radical scavenging effects at 20 mg/mL (85%) which is closer to the control antioxidants (ascorbic acid 97%;BHT 92.4%;BHA 93.6%). Both the ethanol extract (IPS EE and EPS EE) exhibited the highest β-carotene linoleic acid oxidation by recording IC50 values of 0.6 and 0.65 mg/mL respectively. IPS EE (0.59 mg/g) and EPS WE (0.58 mg/g) showed high flavonoid content whereas total phenol content was high in EPS WE (0.85 mg/g) and IPS EE (0.84 mg/g). Total carbohydrate concentrations were high in IPS HWE and IPS CWE with 19.1 and 16.3 mg/mL respectively and these data correlate with the β-glucan content in both extracts. The highest β-glucan content found in IPS CWE and IPS HWE were 12.2 and 11.4% w/w respectively.

一株野生翘鳞香菇的培养特性研究

以野外采集分离的一株野生翘鳞香菇（Lentinus squarrosulus Mont．）为研究对象，探讨了培养基碳源、氮源、碳氮比、pH和温度对翘鳞香菇菌丝生长的影响，同时进行了人工驯化栽培。结果表明，翘鳞香菇的菌丝在生长过程中培养特性明显．培养基最适碳源是葡萄糖或麦芽糖，最适氮源是酵母浸膏，最适碳氮比是10：1—20：1，最适pH为5．5～6．0，最适生长温度是30℃左右。在此培养条件下，试验成功获得了翘鳞香菇的大型子实体。

Characterization and Performance Evaluation of Mycelium-Based Biofoams for Cushioning Materials Using Edible Mushrooms

This study investigated the development of mycelium-based biofoams as sustainable cushioning materials using Pleurotus ostreatus and Lentinus squarrosulus,combined with different sawdust substrates,and subjected to various pressing methods.The results indicated significant effects of mushroom species,sawdust type,and pressing method on the properties of biofoams.Growth rate,morphology,chemical composition,physical and mechanical properties,water resistance,and cushioning factor were evaluated.The results indicated that Lentinus squarrosulus(LS)exhibited faster growth rates(up to 14.37 mm/day)and produced biofoams with superior properties compared to Pleurotus ostreatus(PO).Core wood(CW)sawdust generally resulted in biofoams with lower density(0.1-0.3 g/cm^(3)),lower shrinkage(7.17%-11.41%),and better shock absorption properties(cushion factor of 4.45-4.73).Hot pressing(HO)consistently produced biofoams with higher density(up to 0.31 g/cm3),improved mechanical strength(compression strength up to 0.53 MPa),and enhanced hydrophobicity(water contact angle up to 102.03°)but slightly reduced the shock absorption performance.Biofoam made from LS cultivated on CW sawdust and pressed using hand-packing(HP)exhibited superior shock absorption properties,achieving a cushion factor of 4.45 comparable to expanded polystyrene(EPS)foam.The findings demonstrated that certain combinations of sawdust types and pressing methods can optimize the performance of mycelium-based biofoams,making them viable for sustainable packaging applications.This study highlighted the potential of mycelium biofoams as an eco-friendly alternative to conventional packaging materials,thereby decreasing environmental impact and promoting a sustainable future.