目的分析消化内科门诊胃食管反流样症状患者口服质子泵抑制剂(PPIs)的规范性与合理性,为临床提供参考。方法选取2017年7月1日~2022年12月31日消化内科门诊以胃食管反流样症状为主诉且完善内镜检查及食管24 h pH值监测的患者200例,统计患...目的分析消化内科门诊胃食管反流样症状患者口服质子泵抑制剂(PPIs)的规范性与合理性,为临床提供参考。方法选取2017年7月1日~2022年12月31日消化内科门诊以胃食管反流样症状为主诉且完善内镜检查及食管24 h pH值监测的患者200例,统计患者PPIs的使用情况,分析PPIs用药的合理性和规范性。结果200例患者中,使用PPIs患者有182例,其中102例(56.04%)患者长期服用PPIs时间超过3个月,服用的PPIs中以雷贝拉唑钠肠溶胶囊占比较重,其次为奥美拉唑肠溶胶囊、艾司奥美拉唑肠溶胶囊、泮托拉唑钠肠溶胶囊、兰索拉唑肠溶片;182例患者中有111例不合理用药者(占比60.99%),包括71例预防用药不合理者(占比39.01%)和40例治疗用药不合理者(占比21.98%);111例不合理用药的症状分布中以反流、烧心为主要症状的有63例,占比较重,为56.76%,其次为胸骨后不适、嗳气、腹痛、咳嗽等相关症状;182例使用过PPIs患者中,幽门螺杆菌感染者有74例(40.66%),仅12例(6.59%)在服用PPIs前进行了^(13)C呼气试验的检测,明确了有无幽门螺杆菌感染。结论某院普遍存在PPIs药物不合理应用现象,因此,应加强PPIs的科普与规范化使用。展开更多
Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put...Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put into the same tube to run PCR,and the other one is that primers for target gene and internal standard run PCR in separate tubes.In research of environmental endocrine disruption semiquantitive PCR is a usual means to measure estrogen-like effects which result from endocrine disruptors.In order to discuss advantages and disadvantages of these two methods for further research on endocrine disruption in Carassius auratus,RT-PCR was used to evaluate of expression of 776 bp of estrogen receptor gene(ER gene) in liver after taking the injection of E2 in C.auratus by following these two methods respectively,with 18S as internal standard.The results showed that ER gene and 18S gene were amplified successfully following both methods.The first method-putting primers for different gene in the same tube to run PCR might contain problems such as competition and different plat phase between target gene and internal standard,and it is needed to grope appropriate ratio of primers and numbers of cycles,yet it is more accurate.And another method putting primers for different genes in separate tubes to run PCR is easier because of the absence of no problemsexisted in the first method,whereas it is less accurate than the first one.In addition,based on the results,18S is an ideal internal standard for both methods in the research of estrogen-like effects in C.auratus.展开更多
文摘目的分析消化内科门诊胃食管反流样症状患者口服质子泵抑制剂(PPIs)的规范性与合理性,为临床提供参考。方法选取2017年7月1日~2022年12月31日消化内科门诊以胃食管反流样症状为主诉且完善内镜检查及食管24 h pH值监测的患者200例,统计患者PPIs的使用情况,分析PPIs用药的合理性和规范性。结果200例患者中,使用PPIs患者有182例,其中102例(56.04%)患者长期服用PPIs时间超过3个月,服用的PPIs中以雷贝拉唑钠肠溶胶囊占比较重,其次为奥美拉唑肠溶胶囊、艾司奥美拉唑肠溶胶囊、泮托拉唑钠肠溶胶囊、兰索拉唑肠溶片;182例患者中有111例不合理用药者(占比60.99%),包括71例预防用药不合理者(占比39.01%)和40例治疗用药不合理者(占比21.98%);111例不合理用药的症状分布中以反流、烧心为主要症状的有63例,占比较重,为56.76%,其次为胸骨后不适、嗳气、腹痛、咳嗽等相关症状;182例使用过PPIs患者中,幽门螺杆菌感染者有74例(40.66%),仅12例(6.59%)在服用PPIs前进行了^(13)C呼气试验的检测,明确了有无幽门螺杆菌感染。结论某院普遍存在PPIs药物不合理应用现象,因此,应加强PPIs的科普与规范化使用。
文摘Semiquantitive PCR is a method to evaluate expression of target genes by analyzing RT-PCR products in comparison to standards.Two measures are involved;one is that primers for target gene and internal standard are put into the same tube to run PCR,and the other one is that primers for target gene and internal standard run PCR in separate tubes.In research of environmental endocrine disruption semiquantitive PCR is a usual means to measure estrogen-like effects which result from endocrine disruptors.In order to discuss advantages and disadvantages of these two methods for further research on endocrine disruption in Carassius auratus,RT-PCR was used to evaluate of expression of 776 bp of estrogen receptor gene(ER gene) in liver after taking the injection of E2 in C.auratus by following these two methods respectively,with 18S as internal standard.The results showed that ER gene and 18S gene were amplified successfully following both methods.The first method-putting primers for different gene in the same tube to run PCR might contain problems such as competition and different plat phase between target gene and internal standard,and it is needed to grope appropriate ratio of primers and numbers of cycles,yet it is more accurate.And another method putting primers for different genes in separate tubes to run PCR is easier because of the absence of no problemsexisted in the first method,whereas it is less accurate than the first one.In addition,based on the results,18S is an ideal internal standard for both methods in the research of estrogen-like effects in C.auratus.
