Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we u...Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we used transdermal enhancers to deliverAngong Niuhuang pill by modern technology, which expanded the safe dose range and clinical indications. In this study, Angong Niuhuang stickers administered at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to theDazhui （DU14）, Qihai（RN6） andMingmen （DU4） of rats with chronic cerebral ischemia, for 4 weeks. The Morris water maze was used to determine the learning and memory ability of rats. Hematoxylin-eosin staining and Nissl staining were used to observe neuronal damage of the cortex and hippocampal CA1 region in rats with chronic cerebral ischemia. The middle- and high-dose point application ofAngong Niuhuangstickers attenuated neuronal damage in the cortex and hippocampal CA1 region, and improved the memory of rats with chronic cerebral ischemia with an efifcacy similar to interventions by electroacupuncture at Dazhui （DU14）,Qihai （RN6） andMingmen （DU4）. Our experimental ifndings indicate that point application withAngong Niuhuang stickers can improve cognitive function after chronic cerebral ischemia in rats and is neuroprotective with an equivalent efifcacy to acupuncture.展开更多
In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts fr...In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to Dazhui （DU14）, Qihai （RN6） and Mingmen （DU4）. Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia.展开更多
In this paper, the sticker based DNA computing was used for solving the independent set problem. At first, solution space was constructed by using appropriate DNA memory complexes. We defined a new operation called “...In this paper, the sticker based DNA computing was used for solving the independent set problem. At first, solution space was constructed by using appropriate DNA memory complexes. We defined a new operation called “divide” and applied it in construction of solution space. Then, by application of a sticker based parallel algorithm using biological operations, independent set problem was resolved in polynomial time.展开更多
Sticker model is one of the basic models in the DNA computer models.This model is coded with sin-gle-double stranded DNA molecules.It has the following ad-vantages that the operations require no strands extension and ...Sticker model is one of the basic models in the DNA computer models.This model is coded with sin-gle-double stranded DNA molecules.It has the following ad-vantages that the operations require no strands extension and use no enzymes; Whats more, the materials are reusable. Therefore it arouses attention and interest of scientists in many fields. In this paper, we will systematically analyze the theories and applications of the model,summarize other sci-entists contributions in this field,and propose our research results.This paper is the theoretical portion of the sticker model on DNA computer, which includes the introduction of the basic model of sticker computing. Firstly, we systemati-cally introduce the basic theories of classic models about sticker computing; Secondly, we discuss the sticker system which is an abstract computing model based on the sticker model and formal languages; Finally, extend and perfect the model,and present two types of models that are more exten-sive in the applications and more perfect in the theory than the past models: one is the so-called k-bit sticker model,the other is full-message sticker DNA computing model.展开更多
Sticker model is one of the basic models in the DNA computer models. This model is coded with sin-gle-double stranded DNA molecules. It has the following advantages that the operations require no strands extension and...Sticker model is one of the basic models in the DNA computer models. This model is coded with sin-gle-double stranded DNA molecules. It has the following advantages that the operations require no strands extension and use no enzymes; What抯 more, the materials are reusable. Therefore, it arouses attention and interest of scientists in many fields. In this paper, we extend and improve the sticker model, which will be definitely beneficial to the construction of DNA computer. This paper is the second part of our series paper, which mainly focuses on the application of sticker model. It mainly consists of the following three sections: the matrix representation of sticker model is first presented; then a brief review of the past research on graph and com-binatorial optimization, such as the minimal set covering problem, the vertex covering problem, Hamiltonian path or cycle problem, the maximal clique problem, the maximal independent problem and the Steiner spanning tree problem, is described; Finally a DNA algorithm for the graph iso-morphic problem based on the sticker model is given.展开更多
基金supported by the National Natural Science Foundation of China,No.81403458a grant from Cultivation Project Foundation for Youth Technological Talents of Southern Medical University,No.B1012015
文摘Angong Niuhuang pill, a Chinese materia medica preparation, can improve neurological func-tions after acute ischemic stroke. Because of its inconvenient application and toxic components （Cinnabaris andRealgar）, we used transdermal enhancers to deliverAngong Niuhuang pill by modern technology, which expanded the safe dose range and clinical indications. In this study, Angong Niuhuang stickers administered at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to theDazhui （DU14）, Qihai（RN6） andMingmen （DU4） of rats with chronic cerebral ischemia, for 4 weeks. The Morris water maze was used to determine the learning and memory ability of rats. Hematoxylin-eosin staining and Nissl staining were used to observe neuronal damage of the cortex and hippocampal CA1 region in rats with chronic cerebral ischemia. The middle- and high-dose point application ofAngong Niuhuangstickers attenuated neuronal damage in the cortex and hippocampal CA1 region, and improved the memory of rats with chronic cerebral ischemia with an efifcacy similar to interventions by electroacupuncture at Dazhui （DU14）,Qihai （RN6） andMingmen （DU4）. Our experimental ifndings indicate that point application withAngong Niuhuang stickers can improve cognitive function after chronic cerebral ischemia in rats and is neuroprotective with an equivalent efifcacy to acupuncture.
基金supported by the Research Project of Administration of TCM of Guangdong Province, No. 20111270
文摘In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses （1.35, 2.7, and 5.4 g/kg） were administered to Dazhui （DU14）, Qihai （RN6） and Mingmen （DU4）. Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia.
文摘In this paper, the sticker based DNA computing was used for solving the independent set problem. At first, solution space was constructed by using appropriate DNA memory complexes. We defined a new operation called “divide” and applied it in construction of solution space. Then, by application of a sticker based parallel algorithm using biological operations, independent set problem was resolved in polynomial time.
文摘Sticker model is one of the basic models in the DNA computer models.This model is coded with sin-gle-double stranded DNA molecules.It has the following ad-vantages that the operations require no strands extension and use no enzymes; Whats more, the materials are reusable. Therefore it arouses attention and interest of scientists in many fields. In this paper, we will systematically analyze the theories and applications of the model,summarize other sci-entists contributions in this field,and propose our research results.This paper is the theoretical portion of the sticker model on DNA computer, which includes the introduction of the basic model of sticker computing. Firstly, we systemati-cally introduce the basic theories of classic models about sticker computing; Secondly, we discuss the sticker system which is an abstract computing model based on the sticker model and formal languages; Finally, extend and perfect the model,and present two types of models that are more exten-sive in the applications and more perfect in the theory than the past models: one is the so-called k-bit sticker model,the other is full-message sticker DNA computing model.
文摘Sticker model is one of the basic models in the DNA computer models. This model is coded with sin-gle-double stranded DNA molecules. It has the following advantages that the operations require no strands extension and use no enzymes; What抯 more, the materials are reusable. Therefore, it arouses attention and interest of scientists in many fields. In this paper, we extend and improve the sticker model, which will be definitely beneficial to the construction of DNA computer. This paper is the second part of our series paper, which mainly focuses on the application of sticker model. It mainly consists of the following three sections: the matrix representation of sticker model is first presented; then a brief review of the past research on graph and com-binatorial optimization, such as the minimal set covering problem, the vertex covering problem, Hamiltonian path or cycle problem, the maximal clique problem, the maximal independent problem and the Steiner spanning tree problem, is described; Finally a DNA algorithm for the graph iso-morphic problem based on the sticker model is given.
