Surgical vacuum-assisted closure for treatment of dramatic case of stonefish envenomation

Skin necrosis of the foot, oedema and lymphangitis from stonefish （Synanceia verrucosa） sting are complications well known for a tong time and with potential long-term sequelae. Literature reports of stonefish envenomation give no specific reference on soft tissue management and surgical reconstruction. This is the first report describing a case of foot stonefish envenomation treated by vacuum-assisted closure therapy as an easy to use, accessible and simple adjuvant tool for management of large soft tissue necrosis.

新玫瑰毒鲉毒素马血清抗体制备及抗毒活性研究

目的建立毒鲉毒素(俗称石头鱼毒素)马血清抗体的制备方法并研究其抗毒活性,为毒鲉毒素中毒提供治疗手段。方法利用排阻色谱制备毒鲉毒素,免疫马匹制备毒鲉毒素马抗,再制备不含FC的F(ab’)2并研究其对小鼠的抗毒活性。结果制备了新玫瑰毒鲉毒素(neoVTX)322.6 mg,纯度为88%,制备的马血清抗体原液中的蛋白含量为41 g/L,F(ab’)2的含量为87.05%,IgG含量为1.35%。4 LD50毒鲉粗毒素可被2倍质量的马抗完全中和;3 LD50neoVTX可被3倍质量的马抗完全中和。结论成功制备了毒鲉毒素及其马血清抗体,该抗体具有较高的抗毒活性,为毒鲉毒素中毒提供了治疗手段。