Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In...Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In wheat(Triticum aestivum L.),we found nine genetic loci of tae-miR164(tae-MIR164 a to i)producing two mature sequences that downregulate the expression of three newly identified target genes of TaNACs(TaNAC1,TaNAC11,and TaNAC14)by the cleavage of the respective mRNAs.Overexpression of tae-miR164 or one of its target genes(TaNAC14)demonstrated that the miR164-TaNAC14 module greatly affects root growth and development and stress(drought and salinity)tolerance in wheat seedlings,and TaNAC14 promotes root growth and development in wheat seedlings and enhances drought tolerance,while tae-miR164 inhibits root development and reduces drought and salinity tolerance by downregulating the expression of TaNAC14.These findings identify the miR164-TaNAC14 module as well as other taemiR164-regulated genes which can serve as new genetic resources for stress-resistance wheat breeding.展开更多
【目的】鉴定葡萄miR164s(VvmiR164a/b/c/d)及其靶基因,明确VvmiR164s及其靶基因应答外源赤霉素在葡萄单性结实过程中的调控作用。【方法】以‘魏可’葡萄(Vitis vinifera L. Wink)为试材,利用miR-RACE、PCR、RLM-RACE与PPM-RACE、qRT-...【目的】鉴定葡萄miR164s(VvmiR164a/b/c/d)及其靶基因,明确VvmiR164s及其靶基因应答外源赤霉素在葡萄单性结实过程中的调控作用。【方法】以‘魏可’葡萄(Vitis vinifera L. Wink)为试材,利用miR-RACE、PCR、RLM-RACE与PPM-RACE、qRT-PCR及生物信息学等技术,分析VvmiR164s-VvNAC100模块应答外源赤霉素在葡萄单性结实过程中的时空表达特征及其潜在功能。【结果】赤霉素在花前处理‘魏可’葡萄,可诱导其单性结实,导致葡萄的无核。克隆鉴定了VvmiR164a/b/c/d的精确序列,预测到其4条靶基因VvNAC100-1、VvNAC100-2、VvNAC098、VvNAC021,结合匹配程度与前期数据,在此重点分析靶基因VvNAC100-2,并将其命名为VvNAC100。克隆鉴定靶基因VvNAC100,验证其裂解位点,并对其开展蛋白进化、染色体定位及结构分析。VvNAC100裂解位点位于miRNA 5′端的第9位与第11位,裂解频度为17/20与11/20,定位于葡萄的Chr14上,编码363个氨基酸,含有一个NAM结构域,且其定位于细胞核。VvNAC100蛋白与其他物种氨基酸序列保守性较高,功能相似,其中与辣椒、烟草等物种亲缘关系较近。VvMIR164a/b/c/d的启动子与其靶基因VvNAC100的启动子均包含多种激素作用元件,表明其可能通过响应不同的激素来参与葡萄生长发育的调控。RT-qPCR结果显示,随着葡萄子房的发育,VvmiR164b的表达水平呈下降趋势,其靶基因VvNAC100在子房发育前期呈上升的表达趋势,具有一定的负相关,而VvmiR164a/c/d与VvNAC100表达模式相似,呈现一定的正相关;GA处理后,VvmiR164a/c/d在葡萄子房单性结实过程中的表达极显著地上升,进而也显著抑制了VvNAC100在这一时期的表达,从而促进了VvmiR164a/c/d-VvNAC100表达水平的负相关;但VvmiR164b在GA处理后表现出下降的趋势,且其与VvNAC100的表达水平呈现一定的正相关,表明GA处理增强了VvmiR164a/c/d对VvNAC100的负调控,减弱了VvmiR164b对VvNAC100的负调控作用。【结论】VvNAC100是VvmiR164家族VvmiR164a/b/c/d的真实靶基因;在葡萄单性结实过程中,赤霉素诱导了VvmiR164a/c/d对靶基因VvNAC100的负调控作用,抑制了VvmiR164b对靶基因VvNAC100的负调控作用,VvmiR164a/c/d是VvmiR164家族参与赤霉素诱导葡萄单性结实的主效因子。展开更多
目的TMEM164是TMEM家族的一员,实验基于肿瘤基因组图谱(TCGA)和Oncomine数据库评估了TMEM164在肝细胞癌中表达及临床意义。方法从GDC Data Portal网站(https://portal.gdc.cancer.gov/)下载424例HCC的RNA表达谱和临床病理参数资料。其...目的TMEM164是TMEM家族的一员,实验基于肿瘤基因组图谱(TCGA)和Oncomine数据库评估了TMEM164在肝细胞癌中表达及临床意义。方法从GDC Data Portal网站(https://portal.gdc.cancer.gov/)下载424例HCC的RNA表达谱和临床病理参数资料。其中肝细胞癌(HCC)组织374例,癌旁正常组织50例。借助Oncomine数据库进一步扩大样本量,验证TCGA数据库分析结果是否正确。采用Kaplan-Meier曲线和Cox回归分析TMEM164表达与患者预后的相关性,并进行基因集富集分析(GSEA),探讨其潜在作用机制。结果TMEM164在HCC组织中表达量显著高于癌旁正常组织(P<0.001);TMEM164表达水平与HCC患者的Stage分期和T分期均显著相关(P<0.05);生存分析显示TMEM164高表达患者总生存率显著低于低表达患者(P<0.01);单因素及多因素Cox回归分析结果表明TMEM164高表达可能作为HCC患者预后的独立指标。GSEA表明TMEM164可能通过调控mTOR、ERBB等通路进而调控细胞周期、凋亡、自噬及RNA降解等参与HCC的进程,此外TMEM164还可能作用于与免疫相关的信号通路。结论TMEM164在肝细胞癌中高表达,与患者预后不良相关,可能成为HCC患者的预后标志物及潜在治疗靶点。展开更多
Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to stress.As...Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to stress.As the main target gene of miR164,NAC transcription factors are mainly regulated at the post-transcriptional level.MiR164:NAC module may play an important role in determining the adaptive response of sugarcane to stress.MiR164 has a regulatory effect on the expression of target gene NAC,and may be closely related to the resistance process of sugarcane to abiotic stress,which provides a reference for using miRNA to carry out sugarcane resistance molecular breeding.展开更多
基金financially supported by the National Natural Science Foundation of China(32072003and 32072059)the Key Research and Development Program of Shaanxi Province,China(2021NY-079)。
文摘Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In wheat(Triticum aestivum L.),we found nine genetic loci of tae-miR164(tae-MIR164 a to i)producing two mature sequences that downregulate the expression of three newly identified target genes of TaNACs(TaNAC1,TaNAC11,and TaNAC14)by the cleavage of the respective mRNAs.Overexpression of tae-miR164 or one of its target genes(TaNAC14)demonstrated that the miR164-TaNAC14 module greatly affects root growth and development and stress(drought and salinity)tolerance in wheat seedlings,and TaNAC14 promotes root growth and development in wheat seedlings and enhances drought tolerance,while tae-miR164 inhibits root development and reduces drought and salinity tolerance by downregulating the expression of TaNAC14.These findings identify the miR164-TaNAC14 module as well as other taemiR164-regulated genes which can serve as new genetic resources for stress-resistance wheat breeding.
文摘【目的】鉴定葡萄miR164s(VvmiR164a/b/c/d)及其靶基因,明确VvmiR164s及其靶基因应答外源赤霉素在葡萄单性结实过程中的调控作用。【方法】以‘魏可’葡萄(Vitis vinifera L. Wink)为试材,利用miR-RACE、PCR、RLM-RACE与PPM-RACE、qRT-PCR及生物信息学等技术,分析VvmiR164s-VvNAC100模块应答外源赤霉素在葡萄单性结实过程中的时空表达特征及其潜在功能。【结果】赤霉素在花前处理‘魏可’葡萄,可诱导其单性结实,导致葡萄的无核。克隆鉴定了VvmiR164a/b/c/d的精确序列,预测到其4条靶基因VvNAC100-1、VvNAC100-2、VvNAC098、VvNAC021,结合匹配程度与前期数据,在此重点分析靶基因VvNAC100-2,并将其命名为VvNAC100。克隆鉴定靶基因VvNAC100,验证其裂解位点,并对其开展蛋白进化、染色体定位及结构分析。VvNAC100裂解位点位于miRNA 5′端的第9位与第11位,裂解频度为17/20与11/20,定位于葡萄的Chr14上,编码363个氨基酸,含有一个NAM结构域,且其定位于细胞核。VvNAC100蛋白与其他物种氨基酸序列保守性较高,功能相似,其中与辣椒、烟草等物种亲缘关系较近。VvMIR164a/b/c/d的启动子与其靶基因VvNAC100的启动子均包含多种激素作用元件,表明其可能通过响应不同的激素来参与葡萄生长发育的调控。RT-qPCR结果显示,随着葡萄子房的发育,VvmiR164b的表达水平呈下降趋势,其靶基因VvNAC100在子房发育前期呈上升的表达趋势,具有一定的负相关,而VvmiR164a/c/d与VvNAC100表达模式相似,呈现一定的正相关;GA处理后,VvmiR164a/c/d在葡萄子房单性结实过程中的表达极显著地上升,进而也显著抑制了VvNAC100在这一时期的表达,从而促进了VvmiR164a/c/d-VvNAC100表达水平的负相关;但VvmiR164b在GA处理后表现出下降的趋势,且其与VvNAC100的表达水平呈现一定的正相关,表明GA处理增强了VvmiR164a/c/d对VvNAC100的负调控,减弱了VvmiR164b对VvNAC100的负调控作用。【结论】VvNAC100是VvmiR164家族VvmiR164a/b/c/d的真实靶基因;在葡萄单性结实过程中,赤霉素诱导了VvmiR164a/c/d对靶基因VvNAC100的负调控作用,抑制了VvmiR164b对靶基因VvNAC100的负调控作用,VvmiR164a/c/d是VvmiR164家族参与赤霉素诱导葡萄单性结实的主效因子。
文摘目的TMEM164是TMEM家族的一员,实验基于肿瘤基因组图谱(TCGA)和Oncomine数据库评估了TMEM164在肝细胞癌中表达及临床意义。方法从GDC Data Portal网站(https://portal.gdc.cancer.gov/)下载424例HCC的RNA表达谱和临床病理参数资料。其中肝细胞癌(HCC)组织374例,癌旁正常组织50例。借助Oncomine数据库进一步扩大样本量,验证TCGA数据库分析结果是否正确。采用Kaplan-Meier曲线和Cox回归分析TMEM164表达与患者预后的相关性,并进行基因集富集分析(GSEA),探讨其潜在作用机制。结果TMEM164在HCC组织中表达量显著高于癌旁正常组织(P<0.001);TMEM164表达水平与HCC患者的Stage分期和T分期均显著相关(P<0.05);生存分析显示TMEM164高表达患者总生存率显著低于低表达患者(P<0.01);单因素及多因素Cox回归分析结果表明TMEM164高表达可能作为HCC患者预后的独立指标。GSEA表明TMEM164可能通过调控mTOR、ERBB等通路进而调控细胞周期、凋亡、自噬及RNA降解等参与HCC的进程,此外TMEM164还可能作用于与免疫相关的信号通路。结论TMEM164在肝细胞癌中高表达,与患者预后不良相关,可能成为HCC患者的预后标志物及潜在治疗靶点。
基金Supported by Science and Technology Research Project of Henan Provincial Science and Technology Department(222102110448)Open Research Project of Guangxi Key Laboratory of Sugarcane Genetic Improvement(19-185-24-K-01-01).
文摘Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to stress.As the main target gene of miR164,NAC transcription factors are mainly regulated at the post-transcriptional level.MiR164:NAC module may play an important role in determining the adaptive response of sugarcane to stress.MiR164 has a regulatory effect on the expression of target gene NAC,and may be closely related to the resistance process of sugarcane to abiotic stress,which provides a reference for using miRNA to carry out sugarcane resistance molecular breeding.
