Aim: In order to clarify further the mechanisms underlying the effect of capsulotomy on testicular function, the lev-els of testosterone, LH and FSH were observed. Methods: Intratesticular testosterone levels and LH, ...Aim: In order to clarify further the mechanisms underlying the effect of capsulotomy on testicular function, the lev-els of testosterone, LH and FSH were observed. Methods: Intratesticular testosterone levels and LH, FSH levelsin the peripheral blood of normal, sham-operated and capsulotomized rats were detected by RIA. Results: Aftertesticular capsulotomy, there was a progressive reduction in the testosterone level in the testicular venous blood togetherwith a progressive increase in the LH and FSH levels in the peripheral blood from approximately 30 days post-capsuloto-my. Morphological changes were observed at 5-10 days after capsulotomy, i. e., far ahead of the hormonal changes.Conclusion: The seminiferous tubular damage after testicular capsulotomy was not caused by the reduction in testos-terone, and on the contrary, the hormonal change might be secondary to the morphological alterations. The increase inLH level most likely resulted from a negative feedback influence from the lowered testosterone level, while the increasein FSH secretion may be a feedback signal of the damaged seminiferous tubules. (Asian J Androl 2000 Dec; 2: 257-261 )展开更多
Aim: To demonstrate the effect of capsulotomy on the fertility of male rats. Methods: Testicular capsulotomywas carded out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by coh...Aim: To demonstrate the effect of capsulotomy on the fertility of male rats. Methods: Testicular capsulotomywas carded out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by cohabita-tion with proestrus females overnight and 20 days later, the females were examined for impregnation. Morphologicalchanges at the site of the capsulotomy were observed under light microscope. Results: In rats capsulotomized atDay 60, the fertility was gradually depressed and all the rats completely lost their fertility 2 months post-operation. Atthat time, a partial regeneration of the capsule at the site of capsulotomy was observed. Immature rats capsulotomized atDay 21 were found to possess normal fertility at maturity. The capsulotomy site was almost completely recovered 60days post-operation. Conclusion: In male rats, testicular capsulotomy at the age of Day 60 will damage fertility.However, when capsulotomy is performed at Day 21, fertility is preserve.(Asian J Androl 2001 Mar; 3: 21-25)展开更多
Aim: In order to reveal the histochemical alteration that might occur during the processes of the spermatogenic dis-ruption induced by testicular capsulotomy, the location and alteration of lipid droplets in the semin...Aim: In order to reveal the histochemical alteration that might occur during the processes of the spermatogenic dis-ruption induced by testicular capsulotomy, the location and alteration of lipid droplets in the seminiferous tubules wereobserved in the present study. Methods: Osmium tetroxide was used to demonstrate the lipid droplets in the semi-niferous tubules of capsulotomized and sham-operated control testes. Results: In the seminiferous tubules of thesham-operated rat testes, many small lipid droplets were located close to the basement membrane of the seminiferoustubules. But for the capsulotomized testes, the lipid droplets in the seminiferous tubules had increased in size and num-ber, with many lipid droplets migrated towards the lumen of the tubules. Conclusion: The results indicated that aprogressive fatty degeneration occurred in the seminiferous tubules after testicular capsulotomy.展开更多
Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this pheno...Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this phenomenon, the bindingof endogenous LH to the testes were observed before and after testicular capsulotomy. Methods; Peroxidase-anti-peroxidase (PAP) method was used to detect the binding of LH to the testes in rats. Results; An intense positivestaining of LH was found in the Leydig cells of both the normal and sham-operated control testes. However, at 40 d af-ter operation, the LH immunoreactivity was decreased in the Leydig cells of the capsulotomized testis. By d 60, onlyvery weak positive staining could be observed in these cells. Conclusion; A progressive reduction of endogenousLH binding to the testis occurred in the capsulotomized rat. (Asian J Androl 2001 Sep; 3 : 227 - 230)展开更多
文摘Aim: In order to clarify further the mechanisms underlying the effect of capsulotomy on testicular function, the lev-els of testosterone, LH and FSH were observed. Methods: Intratesticular testosterone levels and LH, FSH levelsin the peripheral blood of normal, sham-operated and capsulotomized rats were detected by RIA. Results: Aftertesticular capsulotomy, there was a progressive reduction in the testosterone level in the testicular venous blood togetherwith a progressive increase in the LH and FSH levels in the peripheral blood from approximately 30 days post-capsuloto-my. Morphological changes were observed at 5-10 days after capsulotomy, i. e., far ahead of the hormonal changes.Conclusion: The seminiferous tubular damage after testicular capsulotomy was not caused by the reduction in testos-terone, and on the contrary, the hormonal change might be secondary to the morphological alterations. The increase inLH level most likely resulted from a negative feedback influence from the lowered testosterone level, while the increasein FSH secretion may be a feedback signal of the damaged seminiferous tubules. (Asian J Androl 2000 Dec; 2: 257-261 )
文摘Aim: To demonstrate the effect of capsulotomy on the fertility of male rats. Methods: Testicular capsulotomywas carded out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by cohabita-tion with proestrus females overnight and 20 days later, the females were examined for impregnation. Morphologicalchanges at the site of the capsulotomy were observed under light microscope. Results: In rats capsulotomized atDay 60, the fertility was gradually depressed and all the rats completely lost their fertility 2 months post-operation. Atthat time, a partial regeneration of the capsule at the site of capsulotomy was observed. Immature rats capsulotomized atDay 21 were found to possess normal fertility at maturity. The capsulotomy site was almost completely recovered 60days post-operation. Conclusion: In male rats, testicular capsulotomy at the age of Day 60 will damage fertility.However, when capsulotomy is performed at Day 21, fertility is preserve.(Asian J Androl 2001 Mar; 3: 21-25)
文摘Aim: In order to reveal the histochemical alteration that might occur during the processes of the spermatogenic dis-ruption induced by testicular capsulotomy, the location and alteration of lipid droplets in the seminiferous tubules wereobserved in the present study. Methods: Osmium tetroxide was used to demonstrate the lipid droplets in the semi-niferous tubules of capsulotomized and sham-operated control testes. Results: In the seminiferous tubules of thesham-operated rat testes, many small lipid droplets were located close to the basement membrane of the seminiferoustubules. But for the capsulotomized testes, the lipid droplets in the seminiferous tubules had increased in size and num-ber, with many lipid droplets migrated towards the lumen of the tubules. Conclusion: The results indicated that aprogressive fatty degeneration occurred in the seminiferous tubules after testicular capsulotomy.
文摘Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this phenomenon, the bindingof endogenous LH to the testes were observed before and after testicular capsulotomy. Methods; Peroxidase-anti-peroxidase (PAP) method was used to detect the binding of LH to the testes in rats. Results; An intense positivestaining of LH was found in the Leydig cells of both the normal and sham-operated control testes. However, at 40 d af-ter operation, the LH immunoreactivity was decreased in the Leydig cells of the capsulotomized testis. By d 60, onlyvery weak positive staining could be observed in these cells. Conclusion; A progressive reduction of endogenousLH binding to the testis occurred in the capsulotomized rat. (Asian J Androl 2001 Sep; 3 : 227 - 230)
