The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investi...The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.展开更多
The Clock gene,a key molecule in circadian systems,is widely distributed in the animal kingdom. We isolated a 936-bp partial c DNA sequence of the C lock gene( Pva- clock) from the darkbarbel catfish P elteobagrus vac...The Clock gene,a key molecule in circadian systems,is widely distributed in the animal kingdom. We isolated a 936-bp partial c DNA sequence of the C lock gene( Pva- clock) from the darkbarbel catfish P elteobagrus vachelli that exhibited high identity with C lock genes of other species of fish and animals(65%–88%). The putative domains included a basic helix-loop-helix(b HLH) domain and two period-ARNT-single-minded(PAS) domains,which were also similar to those in other species of fish and animals. P va- Clock was primarily expressed in the brain,and was detected in all of the peripheral tissues sampled. Additionally,the pattern of P va- Clock expression over a 24-h period exhibited a circadian rhythm in the brain,liver and intestine,with the acrophase at zeitgeber time 21:35,23:00,and 23:23,respectively. Our results provide insight into the function of the molecular C lock of P. vachelli.展开更多
Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16...Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16S rDNA sequence analysis. Five virulence factors were detected, then artificial infection experiment and histopathological method were carried out. These results showed that strain YER6022 was one of Y. enterocolitica family members. In addition, ail, ystb, virF, yadA and HPIint were dectected. In artificial infection experiment, with 80% mortality and 100% morbidity, injected Pelteobagrus vachellis showed red swollen of the anus, abdomen swelling and fim bleeding. There existed serious hyperaemia and edema in kidney, spleen, intestine and liver at the light microscope. Ultrastructural observation indicated that mitochondria of the liver, kidney, spleen and intestine swelled and mitochondrial cristae broke. The data had further shed light on its pathogenicity in Pelteobagrus vachelli. It would benefit for further studies on pathogenesis ofPelteobagrus vachelli infected with Y. enterocolitica.展开更多
基金Supported by the National Natural Science Foundation of China(30371121)the Natural Science Foundation of Chongqing the Educa-tion Committee Foundation of Chongqing~~
文摘The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.
基金Supported by the National Natural Science Foundation of China(No.31402305)the Educational Commission of Sichuan Province of China(No.14ZA0249)+1 种基金the Key Technologies R&D Program of Neijiang,Sichuan,China(No.12108)the College Students’ Scientific Research Project of Neijiang Normal University(Nos.13NSD-66,13NSD-77)
文摘The Clock gene,a key molecule in circadian systems,is widely distributed in the animal kingdom. We isolated a 936-bp partial c DNA sequence of the C lock gene( Pva- clock) from the darkbarbel catfish P elteobagrus vachelli that exhibited high identity with C lock genes of other species of fish and animals(65%–88%). The putative domains included a basic helix-loop-helix(b HLH) domain and two period-ARNT-single-minded(PAS) domains,which were also similar to those in other species of fish and animals. P va- Clock was primarily expressed in the brain,and was detected in all of the peripheral tissues sampled. Additionally,the pattern of P va- Clock expression over a 24-h period exhibited a circadian rhythm in the brain,liver and intestine,with the acrophase at zeitgeber time 21:35,23:00,and 23:23,respectively. Our results provide insight into the function of the molecular C lock of P. vachelli.
基金Supported by the Science&Technology Department of Sichuan Province(2013FZ0014)the Construction Project of the Postgraduate Academic Degree in Southwest University for Nationalities(2013XWD-S071007)
文摘Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16S rDNA sequence analysis. Five virulence factors were detected, then artificial infection experiment and histopathological method were carried out. These results showed that strain YER6022 was one of Y. enterocolitica family members. In addition, ail, ystb, virF, yadA and HPIint were dectected. In artificial infection experiment, with 80% mortality and 100% morbidity, injected Pelteobagrus vachellis showed red swollen of the anus, abdomen swelling and fim bleeding. There existed serious hyperaemia and edema in kidney, spleen, intestine and liver at the light microscope. Ultrastructural observation indicated that mitochondria of the liver, kidney, spleen and intestine swelled and mitochondrial cristae broke. The data had further shed light on its pathogenicity in Pelteobagrus vachelli. It would benefit for further studies on pathogenesis ofPelteobagrus vachelli infected with Y. enterocolitica.
