Core shooting process plays a decisive role in the quality of sand cores, and core box vents distribution is one of the most important factor determining the effectiveness of core shooting process. In this paper, the ...Core shooting process plays a decisive role in the quality of sand cores, and core box vents distribution is one of the most important factor determining the effectiveness of core shooting process. In this paper, the influence of core box vents distribution on the flow dynamics of core shooting process was investigated based on in situ experimental observations with transparent core box, high-speed camera and pressure measuring system. Attention was focused on the variation of both the flow behavior of sand and pressure curves due to different vents distribution. Taking both kinetic and frictional stress into account, a kinetic-frictional constitutive model was established to describe the internal momentum transfer in the solid phase. Two-fluid model(TFM) simulation was then performed and good agreement was achieved between the experimental and simulated results on both the flow behavior of sand and the pressure curves. It was found that vents distribution has direct effect on the pressure difference of different locations in the core box, which determines the buoyancy force exerting on the sand particles and significantly influences the filling process of core sand.展开更多
Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were d...Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria (71%) and Bacillus (23%). Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.展开更多
The approach to determine working frequencies of acoustic in-situ detector for seafloor hydrothermal fluid is presented. Based on the research of deep-sea noise and the sound generated by mid-ocean ridge black smoker ...The approach to determine working frequencies of acoustic in-situ detector for seafloor hydrothermal fluid is presented. Based on the research of deep-sea noise and the sound generated by mid-ocean ridge black smoker hydrothermal vents, and on the hydrothermal-vent animal hearing ranges, coupled with influences of suspended particles of hydrothermal on acoustic attenuation under different frequencies, the optimal frequency range for detection of acoustical signal near black smokers is determined. The optimal frequencies providing the maximum ratio of receiver signal to background noise are obtained. We have developed a laboratory experimental setup for the optimal frequencies selection. In particular, we evaluated time-of-flight performance with respect to the source signal parameters of center frequency and bandwidth. The experimental results confirm the effectiveness of our approach. Current results indicate that individual transducers operated in the range of 18 - 25 kHz are immune to most interfering sounds and suitable for our system.展开更多
Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this ...Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrother- mal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the s- trains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30 MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyro- coccus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demon- strates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment.展开更多
There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-cha...There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-channel chemical sensor to detect such anomalies which can be used in deep-sea at depths of more than 4 000 m. The design allowed five all-solid-state electrodes to be mounted on it and each (apart from one reference electrode) could be changed according to chemicals to be measured. Two experiments were conducted using the chemical sensors. The first was a shallow-sea trial which included sample measurements and in situ monitoring. pH, Eh, CO3^2- and SO4^2- electrodes were utilized to demonstrate that the chemical sensor was accurate and stable outside the laboratory. In the second experiment, the chemical sensor was integrated with pH, Eh, CO3^2- and H2S electrodes, and was used in 29 scans of the seabed along the Southwest Indian Ridge (SWIR) to detect hydrothermal vents, from which 27 sets of valid data were obtained. Hydrothermal vents were identified by analyzing the chemical anomalies, the primary judging criteria were decreasing voltages of Eh and H2S, matched by increasing voltages of pH and CO3^2- . We proposed that simultaneous detection of changes in these parameters will indicate a hydrothermal vent. Amongst the 27 valid sets of data, five potential hydrothermal vents were targeted using the proposed method. We suggest that our sensors could be widely employed by marine scientists.展开更多
The paper presents a physical model of a natural phenomenon, the glow of bubbles at hydrothermal vents formed during underwater volcanic activity. The basis of the model is characteristic non-equilibrium radiation und...The paper presents a physical model of a natural phenomenon, the glow of bubbles at hydrothermal vents formed during underwater volcanic activity. The basis of the model is characteristic non-equilibrium radiation under first order phase transitions that since 2010 has been referred to as the PeTa (Perelman-Tatartchenko) effect. This is the fourth paper in a series developing the model for similar physical phenomena: cavitational luminescence (CL), multi-bubble sonoluminescence (MBSL), single-bubble sonoluminescence (SBSL) and laser-induced bubble luminescence (LIBL). The previous three papers were published during 2017-2018 in this Journal. In the third one we have shown that above mentioned physical effects can be generalized as a phenomenon that we have titled “Vapour bubble luminescence” (VBL). VBL is very clearly represented in a non-equilibrium phase diagram. The essence of VBL is as follows: when there is a local decrease in pressure and/or an increase of temperature in a tiny volume of a liquid occurs, one or several bubbles filled with vapour will appear. Subsequently a very rapid pressure increase and/or temperature decrease in the same volume of liquid leads to supersaturation of the vapour inside the bubble. Upon reaching critical vapor density, instantaneous vapour condensation and emission of the phase transition energy that is accompanied by a flash (this is the PeTa effect) results in a sharp pressure decrease and the bubble collapses due to the pressure drop. This process is accompanied by a shock wave in the liquid. A similar effect occurs if bubbles filled with hot steam, for example from a cappuccino machine, are injected into a relatively large volume of cold water. The VBL model explains all experimental data concerning CL/MBSL/SBSL/LIBL and the relatively new natural phenomenon, the glow of bubbles at hydrothermal vents. Several model experiments demonstrate the PeTa effect under similar conditions. Additionally, we define the PeTa effect in all its manifestations on a non-equilibrium phase diagram. This clarifies which niches can contain VBL processes. We also demonstrate the window of transparency (WT) for the PeTa radiation during crystallization of a supercooled tellurium melt and propose the design of a cavity-free pulsed laser on the basis of similar crystallization processes.展开更多
The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about constructi...The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about construction of experiment on thermal ventilation and the preparation and arrangement of apparatus were discussed,and then the effects of vents on thermal ventilation were studied through experiments,which includes the temperature distribution,the volume of ventilation,the temperature difference between inlets and outlets,the neutral plane,and the effective thermal coefficient of thermal natural ventilation.Based on this,the effects of natural ventilation based on varied area of inlets and outlets and those of vents on one side and on different sides were compared.According to the experiments,the area of inlet vents and outlet vents affect the temperature distribution in chamber,and their effects on ventilation volume are different,but the effects of vents in single side or different sides are the same under the condition that only thermal ventilation is considered.展开更多
Vertical profiles for the uranium-series radioisotopes 210Pb and 210Po were obtained at thetwo hydrothermal vent sites, the Iheya Ridge and the Minami-Ensei Knoll, in the Mid-Okinawa Trough in 1993 and 1994, respectiv...Vertical profiles for the uranium-series radioisotopes 210Pb and 210Po were obtained at thetwo hydrothermal vent sites, the Iheya Ridge and the Minami-Ensei Knoll, in the Mid-Okinawa Trough in 1993 and 1994, respectively. In 1995, both radioisotopes were measured at the Minami-Ensei Knoll a-gain. At the Iheya Ridge, where the hydrothermal activity is not active as reflected by the CH_4 and 222Rn data, both the total 210Pb and 210Po activities show deficiency relative to their parents, and the mean residence time of 210Pb and 210Po is approximately equal to 20 and 2-5 a, respectively. At the Minami-Ensei Knoll, which is characterized by black smokers, the total 210Pb(0.167 × 10^(-3)-2.5 × 10^(-3) Bq/kg) around the plumes is deficient relative to 226Ra but the total 210Po activities (1.83 × 10^(-3) - 2.83 × 10^(-3) Bq/kg) are in excess relative to 210Pb. The 210Po activities are higher than those in the East China Sea and the Okinawa Trough and excess 210Po has been found. The 210Pb/226Ra and 210Po/10Pb activity ratios are 0.1-0.4 and 1.1-7.8, respectively. 210Pb is preferentially scavenged in the hydrothermal plumes. The possible mechanisms governing the concentration of 210Pb in the Minami-Ensei Knoll involve a lateral transport of 210Pb via diffusion. The high 210Po/210Pb activity ratios in the hydrothermal plumes suggest a depletion of 210Pb and addition of 210Po in the hydrothermal vent area.展开更多
As the Okinawa Trough is a back-arc basin in early spreading, modern submarine hydrothermal activity and minerallization have many characteristics which have aroused wide attention. Up to now, three well-known hydro-t...As the Okinawa Trough is a back-arc basin in early spreading, modern submarine hydrothermal activity and minerallization have many characteristics which have aroused wide attention. Up to now, three well-known hydro-thermal venting areas are all located in the middle part of the trough. During two cruise investigations to map and sample the seafloor, numbers of Calyptogena sp. shells were dredged at two sites in the northern trough with comparatively thicker crust and numerous submarine volcanoes. Based on the fact that Calyptogena sp. is only observed around the hydrothermal vents and lives on hydrothermal activities, it is predicted that there is the possibility of modern hydrothermal activities in the northern part of the trough. In this note, the shell is carefully characterized and the sample locations with possible hydrothermal activity are given. It is pointed out that the research of biogenic fossils to trace hydrothermal activity changes in venting time, strength fluctuations, evolution in展开更多
An increase in the crab pot fishery in the East China Sea has caused great pressure on swimming crab Portunus trituberculatus resources.Thus,it is essential to implement suitable measures to release sublegal-sized cra...An increase in the crab pot fishery in the East China Sea has caused great pressure on swimming crab Portunus trituberculatus resources.Thus,it is essential to implement suitable measures to release sublegal-sized crabs to increase the number of recruits for legal-sized crabs.One of the measures considered is the installation of escape vents on crab pots.We tested crab pots with one and two escape vents and compared the catchability and size selectivity of these pots with control pots without an escape vent.Pots with one escape vent located on single side of the pot,top or bottom(SS),and another on both sides at top and bottom edges(BS)were tested in sea trial experiments following typical commercial fishing practice.The results show that both SS pots and BS pots caught significantly smaller number of sublegal-sized crabs(<116 mm carapace width,CW),but the difference in catch between the two types of crab pots is not significant.The 50%selective CWs(CW_(50))and the selective ranges(SRs)of both BS pots are slightly larger than those of SS pots.However,95%confidence intervals of CW_(50)s and SRs are overlapped,indicating that the discrepancy between them is small.Then,in a mixed effect model,the effect of locations of escape vents,taken as the fixed effect,on selectivity parameters and indexes was analyzed by hypothesis testing.The results show that null hypothesis of no effect of location of vents on size selectivity cannot be rejected,indicating that there is no significant difference in size selectivity between the two types of pots for P.trituberculatus.展开更多
With a rich variety of chemical energy sources and steep physical and chemical gradients,hydrothermal vent systems offer a range of habitats to support microbial life.Cultivation-dependent and independent studies have...With a rich variety of chemical energy sources and steep physical and chemical gradients,hydrothermal vent systems offer a range of habitats to support microbial life.Cultivation-dependent and independent studies have led to an emerging view that diverse microorganisms in deep-sea hydrothermal vents live their chemolithoautotrophic,heterotrophic,or mixotrophic life with versatile metabolic strategies.Biogeochemical processes are mediated by microorganisms,and notably,processes involving or coupling the carbon,sulfur,hydrogen,nitrogen,and metal cycles in these unique ecosystems.Here,we review the taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa and emphasize their significant roles in the biogeochemical processes in deep-sea hydrothermal vents.According to the physiology of the targeted taxa and their needs inferred from meta-omics data,the media for selective cultivation can be designed with a wide range of physicochemical conditions such as temperature,pH,hydrostatic pressure,electron donors and acceptors,carbon sources,nitrogen sources,and growth factors.The application of novel cultivation techniques with real-time monitoring of microbial diversity and metabolic substrates and products are also recommended.展开更多
A cold vent is an area where methane-rich fluid seepage occurs. This seepage may alter the local temperature, salinity, and subsequent accumulation of the gas hydrate. Using a kinetic gas hydrate formation model and i...A cold vent is an area where methane-rich fluid seepage occurs. This seepage may alter the local temperature, salinity, and subsequent accumulation of the gas hydrate. Using a kinetic gas hydrate formation model and in situ measurement of tempera- ture, salinity and fluid flux at the southern summit of Hydrate Ridge, we simuIate the gas hydrate accumulation at three distinct fluid sites: clam, bacterial mat, and gas discharge sites. At the clam sites (pore water flux 〈 20 kg m-2 yr-1), pore water advec- tion has little influence on temperature and salinity. However, the salinity and temperature are increased (peak salinity 〉 0.8 tool kg-1) by the formation of gas hydrate causing the base of the hydrate stability zone to move gradually from -115 to -70 meters below seafloor (mbsf). The gas hydrate saturation at the clam sites is relatively high. The water flux at the bacterial mat sites ranges from 100 to 2500 kg m-2 yr-1. The water flow suppresses the increase in salinity resulting in a salinity close to or slightly higher than that of seawater (〈 0.65 mol kg-l). Heat advection by water flow increases temperature significantly, shifting the base of the hydrate stability zone to above 50 or even 3 mbsf. The gas hydrate saturation is relatively low at the bacterial mat site. At the gas discharge sites, the pore water flux could reach 10^10 kg m-2 yr-1, and the temperature could reach that of the source area in 9 min. There is no gas hydrate formation at the gas discharge sites. Our simulative analysis therefore reveals that a lower pore water flux would result in lower salinity, higher temperature, and a shallower base of the hydrate sta- bility zone. This in turn induces a lower gas hydrate formation rate, lower hydrate saturation, and eventually less gas hydrate resources.展开更多
Binary mixtures of alcohols(ethyl,n-butyl and n-amyl)with several aprotic sol- vents,such as acetone,dioxane,THF,DMSO and DMF have been studied systematically by ~1H,^(13)C,^(15)N and ^(17)O NMR measurements.The conce...Binary mixtures of alcohols(ethyl,n-butyl and n-amyl)with several aprotic sol- vents,such as acetone,dioxane,THF,DMSO and DMF have been studied systematically by ~1H,^(13)C,^(15)N and ^(17)O NMR measurements.The concentration dependence of chemical shift of the solvent was used to evaluate equilibrium constants of the complexation of alcohols with the solvents.A relationship between the proton shift of alcohol uncombined with the solvent and its concentration was found,and the fraction of unassociated hydroxyl groups was thus quantitatively described.The effect of solvent on self association and complexation of the alcohol is discussed on the basis of the electron donicity of the solvents.展开更多
Arsenic and sulfur mineralization is a natural phenomenon occurring in hydrothermal systems where parameters like temperature and organic matter(OM)can influence the mobilization of the toxic metalloid in marine envir...Arsenic and sulfur mineralization is a natural phenomenon occurring in hydrothermal systems where parameters like temperature and organic matter(OM)can influence the mobilization of the toxic metalloid in marine environments.In the present study we analyze the influence of temperature and OM(particularly sulfur-containing additives)on As and S precipitation based on the recent discovery of As-rich nanoparticles in the hydrothermal system near the coast of the Greek island Milos.To this end,we experimentally recreate the formation of amorphous colloidal particles rich in As and S via acidification(pH 3–4)of aqueous precursors at various temperatures.At higher temperatures,we observe the formation of monodisperse particles within the first 24 h of the experiment,generating colloidal particles with diameters close to 160 nm.The S:As ratio and particle size of the synthetized particles closely correlates with values for As_(x)S_(y)particles detected in the hydrothermal system off Milos.Furthermore,organic sulfur containing additives(cysteine and glutathione,GSH)are a key factor in the process of nucleation and growth of amorphous colloidal As_(x)S_(y)particles and,together with the temperature gradient present in shallow hydrothermal vents,dictate the stabilization of As-bearing nanomaterials in the environment.Based on these findings,we present a simple model that summarizes our new insights into the formation and mobility of colloidal As in aquatic ecosystems.In this context,amorphous As_(x)S_(y)particles can present harmful effects to micro-and macro-biota not foreseen in bulk As material.展开更多
Deep-sea hydrothermal vents are known as chemosynthetic ecosystems.However,high temperature vents emit light that hypothetically can drive photosynthesis in this habitat.Metagenomic studies have sporadically reported ...Deep-sea hydrothermal vents are known as chemosynthetic ecosystems.However,high temperature vents emit light that hypothetically can drive photosynthesis in this habitat.Metagenomic studies have sporadically reported the occurrence of phototrophic populations such as cyanobacteria in hydrothermal vents.To determine how geographically and taxonomically widespread phototrophs are in deep-sea hydrothermal vents,we collected samples from three niches in a hydrothermal vent on the Southwest Indian Ridge and carried out an integrated metagenomic analysis.We determined the typical community structures of microorganisms found in active venting fields and identified populations of known potential chlorophototrophs and retinalophototrophs.Complete chlorophyll biosynthetic pathways were identified in all samples.By contrast,proteorhodopsins were only found in active beehive smoker diffusers.Taxonomic groups possessing potential phototrophy dependent on semiconductors present in hydrothermal vents were also found in these samples.This systematic comparative metagenomic study reveals the widespread distribution of phototrophic bacteria in hydrothermal vent fields.Our results support the hypothesis that the ocean is a seed bank of diverse microorganisms.Geothermal vent light may provide energy and confer a competitive advantage on phototrophs to proliferate in hydrothermal vent ecosystems.展开更多
The safety valve is an important component to ensure the safe operation of lithium-ion batteries(LIBs).However,the effect of safety valve type on the thermal runaway(TR)and gas venting behavior of LIBs,as well as the ...The safety valve is an important component to ensure the safe operation of lithium-ion batteries(LIBs).However,the effect of safety valve type on the thermal runaway(TR)and gas venting behavior of LIBs,as well as the TR hazard severity of LIBs,are not known.In this paper,the TR and gas venting behavior of three 100 A h lithium iron phosphate(LFP)batteries with different safety valves are investigated under overheating.Compared to previous studies,the main contribution of this work is in studying and evaluating the effect of gas venting behavior and TR hazard severity of LFP batteries with three safety valve types.Two significant results are obtained:(Ⅰ)the safety valve type dominates over gas venting pressure of battery during safety venting,the maximum gas venting pressure of LFP batteries with a round safety valve is 3320 Pa,which is one order of magnitude higher than other batteries with oval or cavity safety valve;(Ⅱ)the LFP battery with oval safety valve has the lowest TR hazard as shown by the TR hazard assessment model based on gray-fuzzy analytic hierarchy process.This study reveals the effect of safety valve type on TR and gas venting,providing a clear direction for the safety valve design.展开更多
Many locations with concentrated hydrates at vents have confirmed the presence of abundant thermogenic gas in the middle of the Qiongdongnan Basin(QDNB).However,the impact of deep structures on gasbearing fluids migra...Many locations with concentrated hydrates at vents have confirmed the presence of abundant thermogenic gas in the middle of the Qiongdongnan Basin(QDNB).However,the impact of deep structures on gasbearing fluids migration and gas hydrates distribution in tectonically inactive regions is still unclear.In this study,the authors apply high-resolution 3D seismic and logging while drilling(LWD)data from the middle of the QDNB to investigate the influence of deep-large faults on gas chimneys and preferred gasescape pipes.The findings reveal the following:(1)Two significant deep-large faults,F1 and F2,developed on the edge of the Songnan Low Uplift,control the dominant migration of thermogenic hydrocarbons and determine the initial locations of gas chimneys.(2)The formation of gas chimneys is likely related to fault activation and reactivation.Gas chimney 1 is primarily arises from convergent fluid migration resulting from the intersection of the two faults,while the gas chimney 2 benefits from a steeper fault plane and shorter migration distance of fault F2.(3)Most gas-escape pipes are situated near the apex of the two faults.Their reactivations facilitate free gas flow into the GHSZ and contribute to the formation of fracture‐filling hydrates.展开更多
Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve...Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve as the two main windows for the material and energy recycling exchange between the lithosphere and outer spheres(biosphere,hydrosphere and atmosphere).However,recent studies have found that the dynamic activities of fluids in these two extreme systems are a crucial source of‘new'carbon in the deep ocean.These carbon sources may become vital contributors to carbon and energy in marine ecosystems,which affect the global deep-sea carbon budget,and the marine ecosystems as well.In this review,we summarize the sources and formation mechanisms of dissolved carbon in the seep fluids from the cold seeps and hydrothermal vents,the contribution of methane oxidation to dissolved carbon,and the characteristics of the carbon isotope composition in the fluid.Furthermore,we analyze and discuss the influence of carbon discharged from seabed on the seawater carbon cycle by comparing and contrasting these two extreme environments.The research may assist in promoting a deeper understanding of the carbon cycle and material interaction in the ocean,particularly further carbon cycle research in the back-arc basin where cold seeps and hydrothermal vents commonly prevail.展开更多
VELA■呼吸机可进行有创和无创通气,适用于急诊病房、重症监护病房(intensive care unit,ICU)、普通病房使用,最小潮气量可至50 mL,适合于儿童患者。内置涡轮依据主板校准数据精准运行,还可根据使用环境进行校准补偿,尤其是在高海拔地区...VELA■呼吸机可进行有创和无创通气,适用于急诊病房、重症监护病房(intensive care unit,ICU)、普通病房使用,最小潮气量可至50 mL,适合于儿童患者。内置涡轮依据主板校准数据精准运行,还可根据使用环境进行校准补偿,尤其是在高海拔地区^([1]),扩大了呼吸机的应用范围。展开更多
基金supported by the Innovation Platform for Through Process Modeling and Simulation of Advanced Materials Processing Technologies(No.2012ZX04012011)the National Science Foundation of China(No.51575304)
文摘Core shooting process plays a decisive role in the quality of sand cores, and core box vents distribution is one of the most important factor determining the effectiveness of core shooting process. In this paper, the influence of core box vents distribution on the flow dynamics of core shooting process was investigated based on in situ experimental observations with transparent core box, high-speed camera and pressure measuring system. Attention was focused on the variation of both the flow behavior of sand and pressure curves due to different vents distribution. Taking both kinetic and frictional stress into account, a kinetic-frictional constitutive model was established to describe the internal momentum transfer in the solid phase. Two-fluid model(TFM) simulation was then performed and good agreement was achieved between the experimental and simulated results on both the flow behavior of sand and the pressure curves. It was found that vents distribution has direct effect on the pressure difference of different locations in the core box, which determines the buoyancy force exerting on the sand particles and significantly influences the filling process of core sand.
基金Project(200805032) supported by Grands from Scientific Research Program of Marine Public Welfare Industry of ChinaProject (DYXM-115-02-2-07) supported by China Ocean Mineral Resources R&D Association (COMRA)
文摘Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria (71%) and Bacillus (23%). Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.
基金supported by the National High Technology Research and Development Program of China(863 Program,Grant No.2007AA09Z213)the National Natural Science Foundation of China(Grant No.40637037)the Science and Technology Program of Zhejiang Province for Qianjiang Professional People(Grant No.2008R10020)
文摘The approach to determine working frequencies of acoustic in-situ detector for seafloor hydrothermal fluid is presented. Based on the research of deep-sea noise and the sound generated by mid-ocean ridge black smoker hydrothermal vents, and on the hydrothermal-vent animal hearing ranges, coupled with influences of suspended particles of hydrothermal on acoustic attenuation under different frequencies, the optimal frequency range for detection of acoustical signal near black smokers is determined. The optimal frequencies providing the maximum ratio of receiver signal to background noise are obtained. We have developed a laboratory experimental setup for the optimal frequencies selection. In particular, we evaluated time-of-flight performance with respect to the source signal parameters of center frequency and bandwidth. The experimental results confirm the effectiveness of our approach. Current results indicate that individual transducers operated in the range of 18 - 25 kHz are immune to most interfering sounds and suitable for our system.
基金The National Basic Research Program of China under contract No.2011CB808800the National Natural Science Foundation of China under contract No.40830213+2 种基金the Science and Technology Commission of Shanghai Municipality under contract No.10JC1406700the State Key Laboratory of Ocean Engineering in China under contract No.GKZD010053-1US-National Science Foundation under contract No.OCE-0728391
文摘Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrother- mal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the s- trains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30 MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyro- coccus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demon- strates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment.
基金The Open Foundation of Laboratory of Marine Ecosystem and Biogeochemistry,SOA under contract No.LMEB201701
文摘There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-channel chemical sensor to detect such anomalies which can be used in deep-sea at depths of more than 4 000 m. The design allowed five all-solid-state electrodes to be mounted on it and each (apart from one reference electrode) could be changed according to chemicals to be measured. Two experiments were conducted using the chemical sensors. The first was a shallow-sea trial which included sample measurements and in situ monitoring. pH, Eh, CO3^2- and SO4^2- electrodes were utilized to demonstrate that the chemical sensor was accurate and stable outside the laboratory. In the second experiment, the chemical sensor was integrated with pH, Eh, CO3^2- and H2S electrodes, and was used in 29 scans of the seabed along the Southwest Indian Ridge (SWIR) to detect hydrothermal vents, from which 27 sets of valid data were obtained. Hydrothermal vents were identified by analyzing the chemical anomalies, the primary judging criteria were decreasing voltages of Eh and H2S, matched by increasing voltages of pH and CO3^2- . We proposed that simultaneous detection of changes in these parameters will indicate a hydrothermal vent. Amongst the 27 valid sets of data, five potential hydrothermal vents were targeted using the proposed method. We suggest that our sensors could be widely employed by marine scientists.
文摘The paper presents a physical model of a natural phenomenon, the glow of bubbles at hydrothermal vents formed during underwater volcanic activity. The basis of the model is characteristic non-equilibrium radiation under first order phase transitions that since 2010 has been referred to as the PeTa (Perelman-Tatartchenko) effect. This is the fourth paper in a series developing the model for similar physical phenomena: cavitational luminescence (CL), multi-bubble sonoluminescence (MBSL), single-bubble sonoluminescence (SBSL) and laser-induced bubble luminescence (LIBL). The previous three papers were published during 2017-2018 in this Journal. In the third one we have shown that above mentioned physical effects can be generalized as a phenomenon that we have titled “Vapour bubble luminescence” (VBL). VBL is very clearly represented in a non-equilibrium phase diagram. The essence of VBL is as follows: when there is a local decrease in pressure and/or an increase of temperature in a tiny volume of a liquid occurs, one or several bubbles filled with vapour will appear. Subsequently a very rapid pressure increase and/or temperature decrease in the same volume of liquid leads to supersaturation of the vapour inside the bubble. Upon reaching critical vapor density, instantaneous vapour condensation and emission of the phase transition energy that is accompanied by a flash (this is the PeTa effect) results in a sharp pressure decrease and the bubble collapses due to the pressure drop. This process is accompanied by a shock wave in the liquid. A similar effect occurs if bubbles filled with hot steam, for example from a cappuccino machine, are injected into a relatively large volume of cold water. The VBL model explains all experimental data concerning CL/MBSL/SBSL/LIBL and the relatively new natural phenomenon, the glow of bubbles at hydrothermal vents. Several model experiments demonstrate the PeTa effect under similar conditions. Additionally, we define the PeTa effect in all its manifestations on a non-equilibrium phase diagram. This clarifies which niches can contain VBL processes. We also demonstrate the window of transparency (WT) for the PeTa radiation during crystallization of a supercooled tellurium melt and propose the design of a cavity-free pulsed laser on the basis of similar crystallization processes.
文摘The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about construction of experiment on thermal ventilation and the preparation and arrangement of apparatus were discussed,and then the effects of vents on thermal ventilation were studied through experiments,which includes the temperature distribution,the volume of ventilation,the temperature difference between inlets and outlets,the neutral plane,and the effective thermal coefficient of thermal natural ventilation.Based on this,the effects of natural ventilation based on varied area of inlets and outlets and those of vents on one side and on different sides were compared.According to the experiments,the area of inlet vents and outlet vents affect the temperature distribution in chamber,and their effects on ventilation volume are different,but the effects of vents in single side or different sides are the same under the condition that only thermal ventilation is considered.
文摘Vertical profiles for the uranium-series radioisotopes 210Pb and 210Po were obtained at thetwo hydrothermal vent sites, the Iheya Ridge and the Minami-Ensei Knoll, in the Mid-Okinawa Trough in 1993 and 1994, respectively. In 1995, both radioisotopes were measured at the Minami-Ensei Knoll a-gain. At the Iheya Ridge, where the hydrothermal activity is not active as reflected by the CH_4 and 222Rn data, both the total 210Pb and 210Po activities show deficiency relative to their parents, and the mean residence time of 210Pb and 210Po is approximately equal to 20 and 2-5 a, respectively. At the Minami-Ensei Knoll, which is characterized by black smokers, the total 210Pb(0.167 × 10^(-3)-2.5 × 10^(-3) Bq/kg) around the plumes is deficient relative to 226Ra but the total 210Po activities (1.83 × 10^(-3) - 2.83 × 10^(-3) Bq/kg) are in excess relative to 210Pb. The 210Po activities are higher than those in the East China Sea and the Okinawa Trough and excess 210Po has been found. The 210Pb/226Ra and 210Po/10Pb activity ratios are 0.1-0.4 and 1.1-7.8, respectively. 210Pb is preferentially scavenged in the hydrothermal plumes. The possible mechanisms governing the concentration of 210Pb in the Minami-Ensei Knoll involve a lateral transport of 210Pb via diffusion. The high 210Po/210Pb activity ratios in the hydrothermal plumes suggest a depletion of 210Pb and addition of 210Po in the hydrothermal vent area.
基金the National Natural Science Foundation of China (Grant Nos. 49625609 and 49421007).
文摘As the Okinawa Trough is a back-arc basin in early spreading, modern submarine hydrothermal activity and minerallization have many characteristics which have aroused wide attention. Up to now, three well-known hydro-thermal venting areas are all located in the middle part of the trough. During two cruise investigations to map and sample the seafloor, numbers of Calyptogena sp. shells were dredged at two sites in the northern trough with comparatively thicker crust and numerous submarine volcanoes. Based on the fact that Calyptogena sp. is only observed around the hydrothermal vents and lives on hydrothermal activities, it is predicted that there is the possibility of modern hydrothermal activities in the northern part of the trough. In this note, the shell is carefully characterized and the sample locations with possible hydrothermal activity are given. It is pointed out that the research of biogenic fossils to trace hydrothermal activity changes in venting time, strength fluctuations, evolution in
基金funded by National Natural Science Foundation of China(31001138)Special Fund for Agro-scientific Research in the Public Interest(201203018).
文摘An increase in the crab pot fishery in the East China Sea has caused great pressure on swimming crab Portunus trituberculatus resources.Thus,it is essential to implement suitable measures to release sublegal-sized crabs to increase the number of recruits for legal-sized crabs.One of the measures considered is the installation of escape vents on crab pots.We tested crab pots with one and two escape vents and compared the catchability and size selectivity of these pots with control pots without an escape vent.Pots with one escape vent located on single side of the pot,top or bottom(SS),and another on both sides at top and bottom edges(BS)were tested in sea trial experiments following typical commercial fishing practice.The results show that both SS pots and BS pots caught significantly smaller number of sublegal-sized crabs(<116 mm carapace width,CW),but the difference in catch between the two types of crab pots is not significant.The 50%selective CWs(CW_(50))and the selective ranges(SRs)of both BS pots are slightly larger than those of SS pots.However,95%confidence intervals of CW_(50)s and SRs are overlapped,indicating that the discrepancy between them is small.Then,in a mixed effect model,the effect of locations of escape vents,taken as the fixed effect,on selectivity parameters and indexes was analyzed by hypothesis testing.The results show that null hypothesis of no effect of location of vents on size selectivity cannot be rejected,indicating that there is no significant difference in size selectivity between the two types of pots for P.trituberculatus.
基金This work was supported by the National Key R&D Program of China(No.2018YFC0310701)the Chinese National Natural Science Foundation(No.91951201)+1 种基金the Scientific Research Foundation of Third Institute of Oceanography,MNR(No.2017003)the Sino-French LIA/IRP 1211 MicrobSea.
文摘With a rich variety of chemical energy sources and steep physical and chemical gradients,hydrothermal vent systems offer a range of habitats to support microbial life.Cultivation-dependent and independent studies have led to an emerging view that diverse microorganisms in deep-sea hydrothermal vents live their chemolithoautotrophic,heterotrophic,or mixotrophic life with versatile metabolic strategies.Biogeochemical processes are mediated by microorganisms,and notably,processes involving or coupling the carbon,sulfur,hydrogen,nitrogen,and metal cycles in these unique ecosystems.Here,we review the taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa and emphasize their significant roles in the biogeochemical processes in deep-sea hydrothermal vents.According to the physiology of the targeted taxa and their needs inferred from meta-omics data,the media for selective cultivation can be designed with a wide range of physicochemical conditions such as temperature,pH,hydrostatic pressure,electron donors and acceptors,carbon sources,nitrogen sources,and growth factors.The application of novel cultivation techniques with real-time monitoring of microbial diversity and metabolic substrates and products are also recommended.
基金supported by National Basic Research Program of China (Grant No.2009CB219508)Chinese Academy of Sciences (Grant No.KZCX2-YW-GJ03)+2 种基金National Natural Science Foundation of China (GrantNos. 91228206 & 40725011)GIGCAS 135 Program (Grant No.Y234021001)Scientific and Technological Program of Guangdong Province (Grant No. 2011A080403021)
文摘A cold vent is an area where methane-rich fluid seepage occurs. This seepage may alter the local temperature, salinity, and subsequent accumulation of the gas hydrate. Using a kinetic gas hydrate formation model and in situ measurement of tempera- ture, salinity and fluid flux at the southern summit of Hydrate Ridge, we simuIate the gas hydrate accumulation at three distinct fluid sites: clam, bacterial mat, and gas discharge sites. At the clam sites (pore water flux 〈 20 kg m-2 yr-1), pore water advec- tion has little influence on temperature and salinity. However, the salinity and temperature are increased (peak salinity 〉 0.8 tool kg-1) by the formation of gas hydrate causing the base of the hydrate stability zone to move gradually from -115 to -70 meters below seafloor (mbsf). The gas hydrate saturation at the clam sites is relatively high. The water flux at the bacterial mat sites ranges from 100 to 2500 kg m-2 yr-1. The water flow suppresses the increase in salinity resulting in a salinity close to or slightly higher than that of seawater (〈 0.65 mol kg-l). Heat advection by water flow increases temperature significantly, shifting the base of the hydrate stability zone to above 50 or even 3 mbsf. The gas hydrate saturation is relatively low at the bacterial mat site. At the gas discharge sites, the pore water flux could reach 10^10 kg m-2 yr-1, and the temperature could reach that of the source area in 9 min. There is no gas hydrate formation at the gas discharge sites. Our simulative analysis therefore reveals that a lower pore water flux would result in lower salinity, higher temperature, and a shallower base of the hydrate sta- bility zone. This in turn induces a lower gas hydrate formation rate, lower hydrate saturation, and eventually less gas hydrate resources.
文摘Binary mixtures of alcohols(ethyl,n-butyl and n-amyl)with several aprotic sol- vents,such as acetone,dioxane,THF,DMSO and DMF have been studied systematically by ~1H,^(13)C,^(15)N and ^(17)O NMR measurements.The concentration dependence of chemical shift of the solvent was used to evaluate equilibrium constants of the complexation of alcohols with the solvents.A relationship between the proton shift of alcohol uncombined with the solvent and its concentration was found,and the fraction of unassociated hydroxyl groups was thus quantitatively described.The effect of solvent on self association and complexation of the alcohol is discussed on the basis of the electron donicity of the solvents.
基金supported by Conicyt through a fellowship to VDT(Programa de Capital Humano Avanzado,BECASChile,CONICYT,72160570 Res.Convenio 7359/2015)by the Deutsche Forschungsgemeinschaft(DFG,Germany)through the Emmy Noether Program(grant BU 2606/1-1 to SIB)。
文摘Arsenic and sulfur mineralization is a natural phenomenon occurring in hydrothermal systems where parameters like temperature and organic matter(OM)can influence the mobilization of the toxic metalloid in marine environments.In the present study we analyze the influence of temperature and OM(particularly sulfur-containing additives)on As and S precipitation based on the recent discovery of As-rich nanoparticles in the hydrothermal system near the coast of the Greek island Milos.To this end,we experimentally recreate the formation of amorphous colloidal particles rich in As and S via acidification(pH 3–4)of aqueous precursors at various temperatures.At higher temperatures,we observe the formation of monodisperse particles within the first 24 h of the experiment,generating colloidal particles with diameters close to 160 nm.The S:As ratio and particle size of the synthetized particles closely correlates with values for As_(x)S_(y)particles detected in the hydrothermal system off Milos.Furthermore,organic sulfur containing additives(cysteine and glutathione,GSH)are a key factor in the process of nucleation and growth of amorphous colloidal As_(x)S_(y)particles and,together with the temperature gradient present in shallow hydrothermal vents,dictate the stabilization of As-bearing nanomaterials in the environment.Based on these findings,we present a simple model that summarizes our new insights into the formation and mobility of colloidal As in aquatic ecosystems.In this context,amorphous As_(x)S_(y)particles can present harmful effects to micro-and macro-biota not foreseen in bulk As material.
基金supported by the National Key Research and Development Program of China(No.2018YFC0309904)the National Natural Science Foundation of China(Nos.91751202,41806174,91751108)+3 种基金the Key Research and Development Program of Hainan Province(No.ZDKJ2019011)Grant Y9719105 from the Institute of Deep-sea Technology Innovation,Chinese Academy of Sciences(IDSTI-CAS)Grant 2019YD16 from Sanya City and Grant INSB-DBM2021support to LIA-MagMC from Centre National de la Recherche Scientifique.
文摘Deep-sea hydrothermal vents are known as chemosynthetic ecosystems.However,high temperature vents emit light that hypothetically can drive photosynthesis in this habitat.Metagenomic studies have sporadically reported the occurrence of phototrophic populations such as cyanobacteria in hydrothermal vents.To determine how geographically and taxonomically widespread phototrophs are in deep-sea hydrothermal vents,we collected samples from three niches in a hydrothermal vent on the Southwest Indian Ridge and carried out an integrated metagenomic analysis.We determined the typical community structures of microorganisms found in active venting fields and identified populations of known potential chlorophototrophs and retinalophototrophs.Complete chlorophyll biosynthetic pathways were identified in all samples.By contrast,proteorhodopsins were only found in active beehive smoker diffusers.Taxonomic groups possessing potential phototrophy dependent on semiconductors present in hydrothermal vents were also found in these samples.This systematic comparative metagenomic study reveals the widespread distribution of phototrophic bacteria in hydrothermal vent fields.Our results support the hypothesis that the ocean is a seed bank of diverse microorganisms.Geothermal vent light may provide energy and confer a competitive advantage on phototrophs to proliferate in hydrothermal vent ecosystems.
基金supported by the National Key R&D Program of China(No.2021YFB2402001)the Postgraduate Innovation and Entrepreneurship Practice Project of Anhui Province(No.2022cxcysj013)+2 种基金the China Postdoctoral Science Foundation(No.2022T150615)the Fundamental Research Funds for the Central Universities(No.WK5290000002)supported by Youth Innovation Promotion Association CAS(No.Y201768)。
文摘The safety valve is an important component to ensure the safe operation of lithium-ion batteries(LIBs).However,the effect of safety valve type on the thermal runaway(TR)and gas venting behavior of LIBs,as well as the TR hazard severity of LIBs,are not known.In this paper,the TR and gas venting behavior of three 100 A h lithium iron phosphate(LFP)batteries with different safety valves are investigated under overheating.Compared to previous studies,the main contribution of this work is in studying and evaluating the effect of gas venting behavior and TR hazard severity of LFP batteries with three safety valve types.Two significant results are obtained:(Ⅰ)the safety valve type dominates over gas venting pressure of battery during safety venting,the maximum gas venting pressure of LFP batteries with a round safety valve is 3320 Pa,which is one order of magnitude higher than other batteries with oval or cavity safety valve;(Ⅱ)the LFP battery with oval safety valve has the lowest TR hazard as shown by the TR hazard assessment model based on gray-fuzzy analytic hierarchy process.This study reveals the effect of safety valve type on TR and gas venting,providing a clear direction for the safety valve design.
基金supported by the National Natural Science Foundation of China(42376221,42276083)Director Research Fund Project of Guangzhou Marine Geological Survey(2023GMGSJZJJ00030)+2 种基金National Key Research and Development Program of China(2021YFC2800901)Guangdong Major Project of Basic and Applied Basic Research(2020B030103003)the project of the China Geological Survey(DD20230064).
文摘Many locations with concentrated hydrates at vents have confirmed the presence of abundant thermogenic gas in the middle of the Qiongdongnan Basin(QDNB).However,the impact of deep structures on gasbearing fluids migration and gas hydrates distribution in tectonically inactive regions is still unclear.In this study,the authors apply high-resolution 3D seismic and logging while drilling(LWD)data from the middle of the QDNB to investigate the influence of deep-large faults on gas chimneys and preferred gasescape pipes.The findings reveal the following:(1)Two significant deep-large faults,F1 and F2,developed on the edge of the Songnan Low Uplift,control the dominant migration of thermogenic hydrocarbons and determine the initial locations of gas chimneys.(2)The formation of gas chimneys is likely related to fault activation and reactivation.Gas chimney 1 is primarily arises from convergent fluid migration resulting from the intersection of the two faults,while the gas chimney 2 benefits from a steeper fault plane and shorter migration distance of fault F2.(3)Most gas-escape pipes are situated near the apex of the two faults.Their reactivations facilitate free gas flow into the GHSZ and contribute to the formation of fracture‐filling hydrates.
基金supported by the Shandong Provincial Natural Science Foundation(No.ZR2021MD049)the National Natural Science Foundation of China(Nos.42176057,and 92358301)the Marine Geological Survey Program(Nos.DD20230402)。
文摘Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve as the two main windows for the material and energy recycling exchange between the lithosphere and outer spheres(biosphere,hydrosphere and atmosphere).However,recent studies have found that the dynamic activities of fluids in these two extreme systems are a crucial source of‘new'carbon in the deep ocean.These carbon sources may become vital contributors to carbon and energy in marine ecosystems,which affect the global deep-sea carbon budget,and the marine ecosystems as well.In this review,we summarize the sources and formation mechanisms of dissolved carbon in the seep fluids from the cold seeps and hydrothermal vents,the contribution of methane oxidation to dissolved carbon,and the characteristics of the carbon isotope composition in the fluid.Furthermore,we analyze and discuss the influence of carbon discharged from seabed on the seawater carbon cycle by comparing and contrasting these two extreme environments.The research may assist in promoting a deeper understanding of the carbon cycle and material interaction in the ocean,particularly further carbon cycle research in the back-arc basin where cold seeps and hydrothermal vents commonly prevail.