Antarctic Paleontological Heritage:Late Cretaceous–Paleogene vertebrates from Seymour(Marambio)Island,Antarctic Peninsula

Antarctica has significant environmental,scientific,historic,and intrinsic values,all of which are worth protecting into the future.This continent has a discrete number of places of scientific interest that exhibit great potential as natural heritage sites;its geodiversity is of fundamental importance to scientific values of the continent,and the pursuit of geological and paleontological knowledge has had a strong influence on its historical values.Seymour Island was once called the‘Rosetta Stone’of Southern Hemisphere paleobiology,because this small island provides the most complete and richly fossiliferous Late Cretaceous–Paleogene sequence in Antarctica.In particular,fossil vertebrates form part of the evidence used in reconstructing the history of life on Antarctica.Paleontological heritage is considered a subset of geo-heritage that embodies both natural and historical components which has received only indirect recognition.Seymour Island is an outstanding paleontological area with high heritage value of its Late Cretaceous/Paleogene vertebrates and should be considered for geo-conservation and protection.This paper reviews vertebrate fossil occurrences and outcrops on Seymour Island and discusses some threats to these fossil sites.

Reptilian vertebrates from Deccan volcanic associated sediments of Malwa Plateau in context to reptiles across Maastrichtian-Paleogene volcanic eruptions in Main Deccan Volcanic Province,India

The enormous eruptive volume of India's Deccan continental flood basalts has been linked to global mass extinction at Cretaceous-Paleogene boundary and destablilisation of both marine and terrestrial ecosystems before and after the Chicxulub bolide impact. Deccan Volcanic Province(DVP) currently covers around 500 000 km^2 of land between latitude 15°10′-24°30′N and longitude 70°10′-82°10′E over central India towards the west coast. The pre-erosional volume is estimated at around 1.6 million km^3 which is one of the largest volcanic eruptions on the Earth. The Deccan lava flows in geographically separated areas in DVP are considered to have separate sources and sites of eruptions and were outpoured in different magnetochrons viz. C30 n to C28 n of Maastrichtian to Paleocene. The Deccan volcanic sequences(DVS) in different provinces are associated with(i) sediments deposited before the arrival(Ground Zero) of the first lava flows-designated as infratrappean or Lameta sediments and(ii) sediments between the lava flows deposited during the period of quiescence at different stratigraphic levels designated as intertrappean(interflow) sediments. The Ground Zero Lameta sediments are the major dinosaur bearing sediments deposited in six inland basins viz. 1. Balasinor-Jhabua;2. Sagar;3. Jabalpur;4. Ambikapur-Amarkantak;5. Salburdi;6. Nand-Dongargaon. The sediments represent mainly the terrestrial ecosystems destabilised by the volcanism and they record history of biotic and environmental changes impacted by the volcanism across the Deccan volcanic eruptions. The titanosaurs, crocodylimorphs and bothremydid turtles survived across the first flows in the region in the C30 n. The skeletal remains of the surviving reptiles are preserved in the Deccan volcanic associated intertrappean lake sediments at different stratigraphic levels higher-up in the sequence. Locally, in the Malwa region the Scincomorp and Anguimorph lizards make their first appearance during the earlier phase of volcanic eruptions in C30 n wherein their well preserved bones are found in the intertrappean/inter lsva lake sediments deposited between the lava flows. The lizard fauna is indicated to have better flourished after the initial Deccan eruptions possibly owing to increased humidity.

The inverted retina and the evolution of vertebrates:an evo-devo perspective

The inverted retina is a basic characteristic of the vertebrate eye.This implies that vertebrates must have a common ancestor with an inverted retina.Of the two groups of chordates,cephalochordates have an inverted retina and urochordates a direct retina.Surprisingly,recent genetics studies favor urochordates as the closest ancestor to vertebrates.The evolution of increasingly complex organs such as the eye implies not only tissular but also structural modifications at the organ level.How these configurational modifications give rise to a functional eye at any step is still subject to debate and speculation.Here we propose an orderly sequence of phylogenetic events that closely follows the sequence of developmental eye formation in extant vertebrates.The progressive structural complexity has been clearly recorded during vertebrate development at the period of organogenesis.Matching the chain of increasing eye complexity in Mollusca that leads to the bicameral eye of the octopus and the developmental sequence in vertebrates,we delineate the parallel evolution of the two-chambered eye of vertebrates starting with an early ectodermal eye.This sequence allows for some interesting predictions regarding the eyes of not preserved intermediary species.The clue to understanding the inverted retina of vertebrates and the similarity between the sequence followed by Mollusca and chordates is the notion that the eye in both cases is an ectodermal structure,in contrast to an exclusively(de novo)neuroectodermal origin in the eye of vertebrates.This analysis places cephalochordates as the closest branch to vertebrates contrary to urochordates,claimed as a closer branch by some researchers that base their proposals in a genetic analysis.

A COMPARATIVE RESEARCH OF ZYMOGRAMS OF LACTATE DEHYDROGENASE IN DIFFERENT TISSUES 0F 25 SPECIES OF VERTEBRATES

The distribution and characteristics of LDH isoenzymes in different tissues of 25spe-cies of vertebrats were comparativety analysed by discontinuous polyacrylamide gel diskelectrophoresis,combined with experiments on heat and urea inhibition.The result showed that indifferent tissues of Cienopharyngodon idellus,Carassius,ayratus,Trichiurus haumete and Miichihysmiiuy in addition to the distribution of LDH isoenzymes,composed of A and B subuits,a E4isoenzyme band,moving very fast to the positive pole,was detectedin the eye tissue of Trichiurushaumele and a F4 isoenzyme hand,moving very slow to the positive pole,was found in liver andkidney of Cienopharyngodon idellus.The A4 isoenzyme band was found in different tisseues ofPleuronichlhys cornulus,inhabiting on the bottom of the ocean.From the amphition to the mammal,with the exception of Hyta arboreu immaculata,Chinnemys reevesii,Trioyx sinensis,Gallus gallusdomeslicus and Corvus macrorhynchos,which have special electrophoretic behaviours.In

New Findings on Devonian“Platypus Fish”Cast Light on Evolution of Modern Jawed Vertebrates

New findings on the brain and inner ear cavity of a 400-million-year-old platypus-like fish cast light on the evolution of modern jawed vertebrates,according to a study led by Dr.ZHU Youan and Dr.LU Jing from the Institute of Vertebrate Paleontology and Paleoanthropology(IVPP)of the Chinese Academy of Sciences(CAS).

NOTES ON SOME PARASITIC NEMATODES OBTAINED FROM VERTEBRATES IN NANKING

In the present paper, 11 species of parasitic nematodes are described, namely: Amidostomum anscris and Ascaridia galli from ducks and fowl; Heteratys golhnae from fowl, H. parisi from pheasants, H. yam sp. nov. from ducks; Cheilospirura hamulosa from fowl; Rhabdias tmnulosa from water snakes, Rh, bicornis and ntpponica from toads; Tanqua anomala from water snakes and Spiroxys japonica from frogs. All specimens were preserved in glycerine-alcohol and studied after clearing in carbol-alcohol.

The signal-transduction pathways of the peripheral olfactory organ and their impairment in vertebrates

Animals rely on olfaction to detect and process invaluable chemical information about their environment.For olfaction to function,chemicals must first be detected,which leads to the activation of signal-transduction pathways at the peripheral olfactory organ.As the olfactory system is in direct contact with the environment,the system is constantly vulnerable to damage by contaminants entering the atmosphere or hydrosphere.Contaminants may have a variety of effects,including disrupting olfactory signals generated during chemical detection,or altering numerous targets along the signal transduction pathway.With any impairment of chemical detection,animals may be unable to rely on olfaction to make correct decisions about their environment and thus their fitness.While other reviews have focussed on olfactory toxicology in general,here we specifically explore how contaminants may affect the signal-transduction pathways at various points and link those changes to olfactory functionality across vertebrates with a focus on fishes.

Meta-analysis reveals that reproductive strategies are associated with sexual differences in oxidative balance across vertebrates

Oxidative stress is a key physiological mechanism underlying life-history tradeoffs. Here, I use meta-analytic techniques to test whether sexual differences in oxidative balance are common in vertebrates and to identify which factors are associated with such differences. The dataset included 732 effect size estimates from 100 articles （82 species）. Larger unsigned effect size （meaning larger sexual differences in a given marker） occurred in： reptiles and fish; those species that do not pro- vide parental care; and oviparous species. Estimates of signed effect size （positive values meaning higher oxidative stress in males） indicated that females were less resistant to oxidative stress than males in： reptiles while males and females were similar in fish, birds, and mammals; those species that do not provide parental care; and oviparous species. There was no evidence for a significant sexual differentiation in oxidative balance in fish, birds, and mammals. Effect size was not associ- ated with： the number of offspring; whether the experimental animals were reproducing or not; biomarker （oxidative damage, non-enzymatic, or enzymatic antioxidant）, the species body mass; the strain （wild vs. domestic）; or the study environment （wild vs. captivity）. Oxidative stress tended to be higher in females than males across most of the tissues analyzed. Levels of residual heterogeneity were high in all models tested. The findings of this meta-analysis indicate that diversification of reproductive strategies might be associated with sexual differences in oxidative balance. This explorative meta-analysis offers a starting platform for future research to investigate the rela-tionship between sex and oxidative balance further.

Foraminiferal biostratigraphy of lignite mines of Kutch,India:Age of lignite and fossil vertebrates

The lignite deposits of Kutch, India are stratigraphically referred to the Naredi Formation and considered to be Early Eocene in age. The biostratigraphy of the older mine at Panandhro and a newly opened mine at Matanomadh has constrained the upper age limit of lignite to the early Bartonian. Its lower age may extend to the late Lutetian. Temporally the formation of lignite corresponds to the warming event of the Middle Eocene and suggests a humid climate at the onset of the warming. The previous palynological studies have already suggested dominance of tropical angiospermic pollen. A diverse assemblage of fossil whales and other vertebrates, many of them supposedly the oldest representatives, were reported from Panandhro mine. These were initially assigned to the Early Eocene and later to the Lute? tian age. The present biostratigraphic study revises their age to the Early Bartonian.

How did Jawed Vertebrates Originate and Rise?

99.8% of extant vertebrate species on Earth, including humans, possess jaws(maxilla and mandible). The group which we belong to is accordingly referred to as jawed vertebrates or gnathostomes. The origin and rise of our group is undoubtedly one of the most critical evolutionary milestones in the history of vertebrates from fish to humans.

Increased complexity of gene structure and base composition in vertebrates

How the structure and base composition of genes changed with the evolution of vertebrates remains a puzzling question. Here we analyzed 895 orthologous protein-coding genes in six multicellular animals： human, chicken, zebrafish, sea squirt, fruit fly, and worm. Our analyses reveal that many gene regions, particularly intron and 3~ UTR, gradually expanded throughout the evolution of vertebrates from their invertebrate ancestors, and that the number of exons per gene increased. Studies based on all protein-coding genes in each genome provide consistent results. We also find that GC-content increased in many gene regions （especially 5＇ UTR） in the evolution of endotherms, except in coding-exons. Analysis of individual genomes shows that 3t UTR demonstrated stronger length and GC-content correlation with intron than 5~ UTR, and gene with large intron in all six species demonstrated relatively similar GC-content. Our data indicates a great increase in complexity in vertebrate genes and we propose that the requirement for morphological and functional changes is probably the driving force behind the evolution of structure and base composition complexity in multicellular animal genes.

Evolutionary innovations of the vertebrates

This review concentrates on the greatest anatomical and morphological evolutionary innovations of the vertebrates.During evolution,many new species of vertebrates evolved and underwent modifications by developing new forms,structures and functions of tissues and organ systems.Evolutionary development of the chordates and vertebrates is herein examined in terms of innovations in their organ systems and organismal complexity.Phases during chordate and vertebrate evolutionary history with unusually high rates of increase in morphological complexity are discussed.These increases in complexity in particular chordates and vertebrates coincided with a likely genome duplication event,which resulted in a large increase in genome size and gene number in early vertebrates,and might indicate an increase in complexity.The Hox and Pax gene families are also discussed because both illustrate the relationships between organismal and molecular complexity.Most unique innovations of vertebrates caused major changes in their organismal complexity,and these changes provided new options for future evolutionary development.

A systematic scheme for monitoring waterbird populations at Shengjin Lake,China:methodology and preliminary results

Changes in the areas and quality of wetland habitat makes it imperative to monitor trends in the number of wintering waterbirds and their distribution in the Yangtze River floodplain, the most important waterbird region in eastern China, so that effective science-based action can be taken to ensure the survival and future recovery of the waterbirds of the region. However, obtaining accurate data on the number of waterbirds and distribution, which can be confidently compared across years and sites, is complicated by a number of factors which can affect count quality. It is essential to employ a survey methodology which maximizes count accuracy and precision and minimizes the bias inherent in counting waterbirds;failure to achieve these goals will lead to incorrect results and analytical problems. Recently we developed a systematic waterbird survey methodology which was tested, with promising results, in the winters of 2008/2009 and 2009/2010 at Shengjin Lake, an important wetland in the Yangtze River floodplain. The methodology involves dividing the lake into discrete survey areas, each containing a number of subareas, with clear boundaries, which were surveyed separately. Data, which included information on counts, distributions, the environment and disturbance, were collected in a standardized manner to maximize precision and minimize bias. We use the results from the surveys of the first two winters to provide examples of how the data can be employed to provide detailed information on the number of waterbirds, their distributions and habitat usage. Finally, we discuss the importance of wider application of the methodology throughout the Yangtze River floodplain to underpin a much needed floodplain-wide waterbird monitoring program.

New findings of Changxingaspis(Xiushuiaspidae,Galeaspida)from the Silurian of Tarim Basin and Zhejiang Province,China

New discovery of the early Silurian fossil fish Changxingaspis(Xiushuiaspidae,Galeaspida),Changxingaspis nianzhongi sp.nov.and C.gui,are described from the Tataertag Formation in Tarim Basin and the Kangshan Formation in Zhejiang Province,respectively.C.nianzhongi mainly differs from C.gui in the shape of the median dorsal opening that is transverse elliptic with a width/length ratio of about 3.0,the long lateral transverse canals extending to the lateral margin of the headshield,and the second lateral transverse canal with dichotomous branchings.Discovery of C.nianzhongi from the Tataertag Formation and C.gui from the Kangshan Formation provide direct evidence on the specific level for the correlation between these two formations,which further supports the Silurian fish-bearing red beds in northwest Zhejiang belonging to the Silurian Lower Red Beds(LRBs)rather than the Upper Red Beds(URBs).Additionally,as the first record of the Changxingaspis in Tarim Basin,it extends the paleogeographical distribution of this genus from the South China Block to the Tarim Block,providing new evidence to support faunal exchanges between these two blocks and the hypothesis of a united Tarim-South China Block during the early Silurian.

Cemented vertebra and adjacent vertebra refractured in a chronic kidney disease-mineral and bone disorder patient: A case report

BACKGROUND Although percutaneous vertebral augmentation(PVA)is a commonly used procedure for treating vertebral compression fracture(VCF),the risk of vertebral refracture should be considered.Chronic kidney disease-mineral and bone disorder(CKD-MBD)is a systemic disease of mineral and bone metabolism.It is associated with an increased risk of fracture.Few studies have reported the use of PVA in patients with CKD-MBD.We herein report a rare case wherein the cemented vertebra and the adjacent vertebra refractured simultaneously in a CKD-MBD patient after PVA.CASE SUMMARY A 74-year-old man suffered from low back pain after taking a fall about 3 wk ago.According to physical examination,imaging and laboratory findings,diagnoses of T12 VCF,CKD-MBD,and chronic kidney disease stage 5 were established.He then received percutaneous vertebroplasty at T12 vertebra.Fourteen weeks later,he presented with T12 and L1 vertebral refractures caused by lumbar sprain.Once again,he was given PVA which was optimized for the refractured vertebrae.Although the short-term postoperative effect was satisfactory,he reported chronic low back pain again at the 3-month follow-up.CONCLUSION It is necessary that patients with CKD-MBD who have received PVA are aware of the adverse effects of CKD-MBD.It may increase the risk of vertebral refracture.Furthermore,the PVA surgical technique needs to be optimized according to the condition of the patient.The medium-and long-term effects of PVA remain uncertain in patients with CKD-MBD.

Vertebral Artery Stenting for Acute Multiple Cerebral Infarctions Caused by Vertebral Artery Dissection After Massage:A Case Report

Vertebral artery dissection is a rare pathology that can cause ischemic stroke in young people.Cervical massage,especially improper pulling manipulation,is a cause of vertebral artery dissection.We present a case of 32-year old woman who developed acute multiple posterior circulation ischemic cerebral infarctions as a result of left vertebral artery V4 segment dissection after receiving neck massage.She underwent emergency vertebral artery stent implantation at the site of the dissection.Symptoms were relieved the day after treatment.The patient recovered without adverse complications or endovascular restenosis in the following year.

Spinal Vertebral Fracture Detection and Fracture Level Assessment Based on Deep Learning

This paper addresses the common orthopedic trauma of spinal vertebral fractures and aims to enhance doctors’diagnostic efficiency.Therefore,a deep-learning-based automated diagnostic systemwithmulti-label segmentation is proposed to recognize the condition of vertebral fractures.The whole spine Computed Tomography(CT)image is segmented into the fracture,normal,and background using U-Net,and the fracture degree of each vertebra is evaluated(Genant semi-qualitative evaluation).The main work of this paper includes:First,based on the spatial configuration network(SCN)structure,U-Net is used instead of the SCN feature extraction network.The attention mechanismandthe residual connectionbetweenthe convolutional layers are added in the local network(LN)stage.Multiple filtering is added in the global network(GN)stage,and each layer of the LN decoder feature map is filtered separately using dot product,and the filtered features are re-convolved to obtain the GN output heatmap.Second,a network model with improved SCN(M-SCN)helps automatically localize the center-of-mass position of each vertebra,and the voxels around each localized vertebra were clipped,eliminating a large amount of redundant information(e.g.,background and other interfering vertebrae)and keeping the vertebrae to be segmented in the center of the image.Multilabel segmentation of the clipped portion was subsequently performed using U-Net.This paper uses VerSe’19,VerSe’20(using only data containing vertebral fractures),and private data(provided by Guizhou Orthopedic Hospital)for model training and evaluation.Compared with the original SCN network,the M-SCN reduced the prediction error rate by 1.09%and demonstrated the effectiveness of the improvement in ablation experiments.In the vertebral segmentation experiment,the Dice Similarity Coefficient(DSC)index reached 93.50%and the Maximum Symmetry Surface Distance(MSSD)index was 4.962 mm,with accuracy and recall of 95.82%and 91.73%,respectively.Fractured vertebrae were also marked as red and normal vertebrae were marked as white in the experiment,and the semi-qualitative assessment results of Genant were provided,as well as the results of spinal localization visualization and 3D reconstructed views of the spine to analyze the actual predictive ability of the model.It provides a promising tool for vertebral fracture detection.

Game-changing insights on vertebral skeletal stem cells in bone metastasis and therapeutic horizons

Greenblatt and his team have unveiled vertebral skeletal stem cells(vSSCs)as a critical player in the landscape of bone metastasis.This commentary delves into the transformative discoveries surrounding vSSCs,emphasizing their distinct role in bone metastasis compared to other stem cell lineages.We illuminate the unique properties and functions of vSSCs,which may account for the elevated susceptibility of vertebral bones to metastatic invasion.Furthermore,we explore the exciting therapeutic horizons opened by this newfound understanding.These include potential interventions targeting vSSCs,modulation of associated signaling pathways,and broader implications for the treatment and management of bone metastasis.By shedding light on these game-changing insights,we hope to pave the way for novel strategies that could revolutionize the prognosis and treatment landscape for cancer patients with metastatic bone disease.

Percutaneous kyphoplasty in the treatment of Kümmell disease in lumbar scoliosis:A case report

BACKGROUND Due to mechanical imbalance in the spine,elderly scoliosis patients tend to develop vertebral fracture nonunion,i.e.,Kümmell disease,when osteoporotic vertebral compression fractures occur.However,accompanying vertebral rotational deformities make surgical procedures challenging risky.Such patients are usually compelled to undergo conservative treatment and there are very few reports on minimally invasive surgeries for them.We first-time report a patient with Kümmell disease and lumbar scoliosis treated with percutaneous kyphoplasty(PKP)under O-arm guidance.CASE SUMMARY An 89-year-old female was admitted to the hospital due to delayed low back pain after a fall.She was diagnosed with Kümmell disease based on physical and radiologic examinations.The patient experienced severe scoliosis and subsequently underwent O-arm-guided kyphoplasty,resulting in a significant alleviation of low back pain.CONCLUSION PKP has good efficacy in treating Kümmell disease.However,surgical risks are elevated in scoliosis patients with Kümmell disease due to the abnormal anatomical structure of the spine.O-arm assisted operations play a crucial role in decreasing surgical risks.

Factors influencing further vertebral height loss following percutaneous vertebroplasty in osteoporotic vertebral compression fractures:A 1-year follow-up study

BACKGROUND Osteoporotic vertebral compression fractures(OVCFs)contribute to back pain and functional limitations in older individuals,with percutaneous vertebroplasty(PVP)emerging as a minimally invasive treatment.However,further height loss post-PVP prompts investigation into contributing factors.AIM To investigate the factors associated with further height loss following PVP with cement augmentation in OVCF patients.METHODS A total of 200 OVCF patients who underwent successful PVP between January 2021 and December 2022 were included in this study.“Further height loss”during 1 year of follow-up in OVCF patients with bone edema was defined as a vertical height loss of≥4 mm.The study population was divided into two groups for analysis:The“No Further Height Loss group(n=179)”and the“Further Height Loss group(n=21).”RESULTS In comparing two distinct groups of patients,significant differences existed in bone mineral density(BMD),vertebral compression degree,prevalence of intravertebral cleft(IVF),type of bone cement used,and cement distribution patterns.Results from binary univariate regression analysis revealed that lower BMD,the presence of IVF,cleft distribution of bone cement,and higher vertebral compression degree were all significantly associated with further height loss.Notably,the use of mineralized collagen modified-poly(methyl methacrylate)bone cement was associated with a significant reduction in the risk of further height loss.In multivariate regression analysis,lower BMD and the presence of IVF remained significantly associated with further height loss.CONCLUSION Further height loss following PVP in OVCF patients is influenced by a complex interplay of factors,especially lower BMD and the presence of IVF.These findings underscore the importance of assessing and managing these factors when addressing height loss following PVP in OVCF patients.