The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest office Oryza sativa L. in China. Little is known about the interactions of this weevil with ind...The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest office Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to flee, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the flee. At tillefing stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2-4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.展开更多
Samples of pheromone lures used in boll weevil, Anthonomus grandis (Boheman), eradication programs are routinely analyzed by Gas Chromatography (GC) to ensure lures are adequately dosed with grandlure, the synthet...Samples of pheromone lures used in boll weevil, Anthonomus grandis (Boheman), eradication programs are routinely analyzed by Gas Chromatography (GC) to ensure lures are adequately dosed with grandlure, the synthetic aggregation phero- mone produced by male weevils. However, preparation of GC samples is tedious, time consuming, and requires a moderate level of experience. We examined the use of a commercially-available electronic nose (e-nose) for rapidly assessing the grandlure contents of lures. The e-nose was trained to recognize headspace collections of grandlure emitted from new lures and after lures were aged under field conditions for 4 d, 7 d, 10 d, and 14 d. Based on cross-validation of the training set, the e-nose was 82% accurate in discriminating among the different age classes of lures. Upon sampling headspace collections of pheromone from a different set of field-aged lures, the e-nose was 〈50% accurate in discriminating 4 d, 7 d, and 10 d aged lures from the other age classes of lures. However, the e-nose identified new and 14 d aged lure samples with 100% accuracy. In light of these findings, e-nose technology shows considerable promise as an alternative approach for rapidly assessing the initial grandlure contents of lures used in boll weevil eradication programs.展开更多
Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Metho...Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Method: The activities were evaluated using standard protocols.In these bioassays, the cowpea seeds were used directly as an insect feed. The activity of the extract and isolated compounds were tested at concentrations of 100, 200 and 300 μg/mL and compared to neem oil and cinnamaldehyde(as standard positive controls). Phytochemical analysis of the ethyl acetate extract was done through a number of chromatographic techniques and the structures of the isolated compounds were established through comprehensive spectroscopic analysis including 2 D-NMR and ESI-MS studies.Results: Fractionation of the active ethyl acetate extract resulted in the isolation of one known anthraquinone,aurantio-obtusin(1) and a novel compound that was named as cassiatorin(2). Compounds1 and 2 showed comparable insect antifeedant properties with the positive controls while their insecticidal and ovipositor deterrent effects were far superior to the standard controls.Conclusions: It is thus concluded that Senna tora extracts and the isolated compounds(1 and2) may be employed in the postharvest management of stored cowpea seeds and as other crop protectants.展开更多
The Kingdom of Saudi Arabia is one of the leading date producing countries. Unfortunately, this important fruit crop is under great threat from the red palm weevil (RPW) (Rhynchophorus ferrugineus), which is a hig...The Kingdom of Saudi Arabia is one of the leading date producing countries. Unfortunately, this important fruit crop is under great threat from the red palm weevil (RPW) (Rhynchophorus ferrugineus), which is a highly invasive pest. Several techniques, including visual inspection, acoustic sensors, sniffer dogs, and pheromone traps have been tried to detect the early stages of a RPW infestation; however, each method has suffered certain logistical and implementation issues. We have applied laser induced breakdown spectroscopy (LIBS) for the early detection of RPW infestation. Through the analysis of the observed LIBS spectra of different infested and healthy samples, we have found presence of Ca, Mg, Na, C, K elements and OH, CN molecules. The spectra also reveal that with the population growth of the pest, the intensity of Mg and Ca atomic lines in LIBS spectra increases rapidly. Similar behavior is observed in the molecular lines of LIBS spectra. The obtained results indicate that the LIBS technique can be used for the early detection of RPW infestation without damaging the date palms.展开更多
The red palm weevil (RPW) Rhynchophorusferrugineus is one of the most invasive insect pests causing massive damage on date palms over the world. Synthetic pyrethroid insecticide beta-cyfluthrin has been evaluated ag...The red palm weevil (RPW) Rhynchophorusferrugineus is one of the most invasive insect pests causing massive damage on date palms over the world. Synthetic pyrethroid insecticide beta-cyfluthrin has been evaluated against developmental stages of RPW under laboratory conditions. These stages were exposed to residual film of the insecticide on transparent cups using a Potter precision laboratory spray tower. Percent mortality of males and females were recorded 0.5, 2.0 and 24 h after treatment. Furthermore, date palm offshoots were infested in mesh house, two months later, offshoots treated with 300 ppm (p.g/mL) beta-eyfluthrin using two methods dipping and injection. Bioassay test showed beta-cyfluthrin at 300 ppm caused 86,7% and 93,3% mortality against males and females after 2 h respectively with LC50 135.74 and 109.4 ppm. By increasing the concentration at 400 ppm, mortality reached 100% against males and females after 24 h, Developmental stages, eggs and larvae were more sensitive toward the insecticide, where at 120 ppm no eggs hatched after 96 h and 86.7% mortality was recorded at 200 ppm against larvae after 30 rain with LC50 93.19 ppm. In addition, dipping method of date palm offshoots in 300 ppm beta-cyfluthrin was a very convenient way to control all RPW stages compared to injection method. So, it possible to recommend this method for treating the date palm offshoots in such insecticide as one of the most important elements of integrated pest management for standardizing insecticide based quarantine protocols.展开更多
The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), (Coleoptera, Curculionidae, Rhynchophorinae), is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several...The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), (Coleoptera, Curculionidae, Rhynchophorinae), is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several countries of the Mediterranean Basin during the last three decades where it attacks palm trees. During the last three decades, multiple introductions of RPW to the Middle East, Europe and Caribbean (Island of Curacao, Netherland Antilles), Lebanon and United States of America (Laguna Beach, Orange County, California) (USA) have occurred and the RPW is now a serious pest of many palm species. Duration of all life parameters varies significantly where the entire life cycle takes about 45 to 298 days. Different artificial diets were developed and were tested for mass rearing of RPW. Control of RPW is difficult due to the concealed nature of the life cycle of the pest. Integrated Pest Management (IPM) strategy has been applied successfully to deal with RPW problem. The IPM strategy consists of various preventive and curative methods; those methods were categorized in nine categories in this review. The main objective of this work was to review the existing knowledge on RPW's different aspects, with an ultimate aim of revealing the actual situation of the research on RPW.展开更多
Plant diseases generate challenging problems in commercial, agriculture and pose real economic threats to both conventional and organic farming systems. The red palm weevil (Rhynchophorus ferrugineus) (RPW) is one of ...Plant diseases generate challenging problems in commercial, agriculture and pose real economic threats to both conventional and organic farming systems. The red palm weevil (Rhynchophorus ferrugineus) (RPW) is one of the most destructive pests of palms in the world. Nowadays, control methods revolve around treatments based on chemicals, biotechnological systems using semi-chemicals or the development of the sterile insect technique (hardly sustainable at this time) and biological control. Biological control as the use of natural microorganisms, extracted products from microorganisms or genetically improved to resist or eliminate of pathogens. Our aim was to evaluate the entomopathogenicity of indigenous Beauveria bassiana and Metarhizium anisopliae obtained in Gaza strip against larvae and adults of R. ferrugineus in order to identify indigenous strains potentially suitable for Red Palm Weevil biological control. B. bassiana & M. anisopliae were isolated from larvae and adult dead of RPW from different position of Gaza strip. Morphological analysis of the isolated fungi and molecular identification was determined using PCR technique. Also, the efficiency of the isolated fungi were evaluated under lab conditions and optimized as a biological agent. On the anther hand, the ability of treated RPW male to infect females is examined and calculated using Abbott's formula. Our results showed that the B. bassiana and M. anisopliae exhibited a good biological control agent against larvae and adults of RPW. The pathogenicity of the two most virulent isolates and the toxicity assay on larvae showed a highest mortality percentage nearly to 100% by 6 days after spraying the larvae with 3.4 × 108 spores/ml of B. bassiana. The mortality percentage reaches to 90% after spraying the larvae with 3.6 × 108 spores/ml of M. anisopliae. The mortality for the adults treated with pesticide arrives to 50% and the control group 10% at the same time. The results revealed that the infection of the adult males by Entomopathogenic fungi (EPF) can be disseminated into the healthy population, after RPW treatment with B. bassiana and M. anisopliae. Our research concludes that B. bassiana and M. anisopliae locally isolated can be used as biological very effective.展开更多
The behavior of osier weevil’s adult was studied at Lindian County in Heilongjiang Provillce in 1987-90. Osier weevil started emergence in the last ten days in July and peaked in the tirst and second ten days in Augu...The behavior of osier weevil’s adult was studied at Lindian County in Heilongjiang Provillce in 1987-90. Osier weevil started emergence in the last ten days in July and peaked in the tirst and second ten days in August. Emergence on the susceptible species, such as Populus berolinensis, is 5 to 10 days earlier than that on the species of pest-resistance such as P. xiaohei. The adult move slowly, and it spreads or distributes mainly by crawling or by carrying of human beings. Osier weevil has a selection on host species tbr intake nourishment. According to the degree of being addicted, the series can be arranged as Populus berolinensis. Populus staline, Populus pesudo simonii× nigra,Populus xiaohei, Populus pachermis. The adult is likely to dwell on trunks and likely to eat slender and tender branches. Osier weevil makes a choice of host plants to lay eggs. The adult laid more eggs on the P.berolinensis than on P. xiaohei or willow. Most of the eggs laid on the young trees were distributed blow 2 m height. As tree’s age increase, the eggs’ spots get higher and higher. The eggs laid on the eastern and the southern sides of ti1e trunk are more than that laid on the West and the North. P.berolinensis and P. staline are the source trees of osier weevil in artificial Stands in Western Heilongjiang. Cutting the pest source tree, planting pest-resistance species, strengthening the quarantine of the forest plants, and controlling of the diseases and pests timely have very important significance to prevent the occtirrence and damage of this pest.展开更多
Pine-cone weevil (Pissodes validirostris Gyll.) is one of the most important pests of Scots pine (Pinus syvestris var. mongolica). The quality and quantity of cone were damaged seriously by this pest. In order to prov...Pine-cone weevil (Pissodes validirostris Gyll.) is one of the most important pests of Scots pine (Pinus syvestris var. mongolica). The quality and quantity of cone were damaged seriously by this pest. In order to provide a foundation for the control of this pest, the prediction of occurrence date of pine-cone weevil was studied with the methods of time of undergo, the effective accumulative temperture and the phenology forecast. The occurrence quantity was studied on the basis of the rules of the population dynamic and the environmental influences on this pest.展开更多
A distribution map of osier weevil (Crytorrynchus lapathi L.) was drown up based on widely collecting information and field survey. The results showed that Osier weevil has a widespread in China, stretching from 33&#...A distribution map of osier weevil (Crytorrynchus lapathi L.) was drown up based on widely collecting information and field survey. The results showed that Osier weevil has a widespread in China, stretching from 33°21' to 51°42' N latitude and 83°00' to 132°58' E longitude, and distributes in forms of big or small patches or sports uncontinously. According to the analysis of meteorological data, the temperature and humidity threshold for osier weevil's distribution were determined by methods of PCA (Principle Component Analysis) and RA(Relativity Analysis): January temperature is -30~ 0℃. Annual temperature -4~13℃, Annual precipitation from 411~ 1,136 min.The areas with January temperature under 0℃, annual temperature above 0℃ and annual precipitation of 400~ 800 mm are the optimum distributing places for osier weevil in China.展开更多
The efficacy of grass carp Ctenopharyngodon idella (Cyprinidae) and weevils Neochetina spp. (Curculionidae) to control the aquatic weed, water hyacinth, is investigated in a square net cage (happas) setting at a...The efficacy of grass carp Ctenopharyngodon idella (Cyprinidae) and weevils Neochetina spp. (Curculionidae) to control the aquatic weed, water hyacinth, is investigated in a square net cage (happas) setting at a farm in Cuddalore District, South India. This novel combination of insects and fish is found to be superior to individual treatments for controlling the weed growth within ll0 d. The biomass of the weed, number of plants, percentage of flowered plants and chlorophyll contents were studied. The weed biomass is reduced from 5 kg (day 1) to 0.33 kg (day 110) when exposed to grass carp and weevils. The number of plants is reduced to 0.75 in grass carp and weevil exposed happas, while it is 741.5 in the control. The mean number of leaves per plant is also reduced. In addition, the chlorophyll a and b are significantly reduced in happas exposed to the combination of fish and insects when compared to the other treatments. Based on the results of this study, we consider the combined use of grass carp and weevils to be more efficient and sustainable for managing water hyacinths than the use of these organisms individually.展开更多
Insects of the genus Sitophilus are among the most destructive pests in rice storage, and the best strategy to control it is to use resistant genotypes. In this study, 26 upland rice elite lines were evaluated for Sit...Insects of the genus Sitophilus are among the most destructive pests in rice storage, and the best strategy to control it is to use resistant genotypes. In this study, 26 upland rice elite lines were evaluated for Sitophilus weevil reaction on grain weight. The seeds were placed in plastic recipients stored in a room with temperature and lightning simulating a warehouse environment. The number of living weevils and the grain weight were obtained in two evaluations, one 35 days after storage, and the other 35 days after infestation. The lines differed statistically for number of living weevils and for grain weight in both evaluations. The correlations between these two characters were -?0.99 and -?0.47 for the first and the second evaluation (P < 0.05), respectively. The lines BRS Pepita, AB 112089 and AB 112090 were the most susceptible. Seventeen of the twenty-six elite lines were resistant in both evaluations and could be successfully used in upland rice breeding programs.展开更多
[ Objective] Coffee bean weevil (Araecerusfasciculatus De Geer) is a worldwide important pest in storehouse, which distributes in tropical and subtropi- cal region, having overlapping ecological zone with the import...[ Objective] Coffee bean weevil (Araecerusfasciculatus De Geer) is a worldwide important pest in storehouse, which distributes in tropical and subtropi- cal region, having overlapping ecological zone with the important biofuel plant Jatropha curcas L.. The paper was to investigate the damage of coffee bean weevil on J. curcas. [Method] Taking planting area in Luodian County of Guizhou Province as the investigation point, the forest stand of J. curcas in field and the indoor stored fruits were investigated, and the occurrence condition and damage consequence of the pest were grasped. Furthermore, the taxonomic status of the pest was also confirmed. [ Result] Coffee bean weevil had common distribution in planting area of J. curcas in Luodian, which was found to cause damage beth in field and indoor condition. The adults of coffee bean weevil fed on fungi with little direct damage on the fruit of J. curcas. However, the adults of the post laid their eggs in- side the peel of fruit, and the larvae hatched and fed inside the peel, resulting in the collapse of the peels, thus causing direct damage on the fruit. In addition, coffee bean weevil might have series of potential damages including direct feeding on seeds, spreading diseases, and posing damage on other economic crops in pro- duction area, etc.. [ Conclusion] J. curcas was an important new host for coffee bean weevil. The pest had certain damage on the plant, which also had potential damage on plant products and other economic crops. The research and control efforts on coffee bean weevil should be strengthened.展开更多
Thai rice is favored by large numbers of consumers of all continents because of its excellenttaste,fragrant aroma and fine texture.Among all Thai rice varieties,Thai Hommal rice is themost preferred.Classification of ...Thai rice is favored by large numbers of consumers of all continents because of its excellenttaste,fragrant aroma and fine texture.Among all Thai rice varieties,Thai Hommal rice is themost preferred.Classification of rice as premium quality requires that almost all grain kernels ofthe rice be perfectly whole with only a small quantity of foreign particles.Of all the foreignpartidles found in rice,rice weevils can wreck severest havoc on the quality and quantity of ricesuch thatpremiumitograde rice.It is widely known that ricemillers adoptt the birth and propagation of riceweevils,rice which end up inthebodyweevilscould beapproxilplied and thereby nooverdosantity of rice weevilsinboth Inear infrared spec-TRtroscop)spectrometer was usedinthis researchal 2500 nm.A total of 20 levels of riceweevil infestational mature rice weevils were appliedto 1680 rice samples.The spectral data and quantity of weevils are analyzed by partial leastsquare regression(PLSR)to establish the model for prediction.The results show that the modelis able to estimate the quantity of weevils in milled Hommali rice and brown Hommali rice withhigh Ral of 0.96 and 0.90,high RPD of 6.07 and 3.26 and smalil bias of 2.93 and 2.94,respectively.展开更多
Smart precision agriculture utilizes modern information and wireless communication technologies to achieve challenging agricultural processes.Therefore,Internet of Things(IoT)technology can be applied to monitor and d...Smart precision agriculture utilizes modern information and wireless communication technologies to achieve challenging agricultural processes.Therefore,Internet of Things(IoT)technology can be applied to monitor and detect harmful insect pests such as red palm weevils(RPWs)in the farms of date palm trees.In this paper,we propose a new IoT-based framework for early sound detection of RPWs using fine-tuned transfer learning classifier,namely InceptionResNet-V2.The sound sensors,namely TreeVibes devices are carefully mounted on each palm trunk to setup wireless sensor networks in the farm.Palm trees are labeled based on the sensor node number to identify the infested cases.Then,the acquired audio signals are sent to a cloud server for further on-line analysis by our fine-tuned deep transfer learning model,i.e.,InceptionResNet-V2.The proposed infestation classifier has been successfully validated on the public TreeVibes database.It includes total short recordings of 1754 samples,such that the clean and infested signals are 1754 and 731 samples,respectively.Compared to other deep learning models in the literature,our proposed InceptionResNet-V2 classifier achieved the best performance on the public database of TreeVibes audio recordings.The resulted classification accuracy score was 97.18%.Using 10-fold cross validation,the fine-tuned InceptionResNet-V2 achieved the best average accuracy score and standard deviation of 94.53%and±1.69,respectively.Applying the proposed intelligent IoT-aided detection system of RPWs in date palm farms is the main prospect of this research work.展开更多
The Central Bank of Nigeria Anchored Borrowers Programme efforts geared towards massive production of cowpea and sustainable food security is challenged with post-harvest losses especially due to insect pest infestati...The Central Bank of Nigeria Anchored Borrowers Programme efforts geared towards massive production of cowpea and sustainable food security is challenged with post-harvest losses especially due to insect pest infestation in storage. So also, chemical method of pest control posed more health and ecological challenges than food insecurity. This work investigated the effect of Piper guineense on cowpea storage weevil at the agronomy laboratory of the Lagos State Polytechnic, Ikorodu. Contact toxicity of P. guineense at 0.2, 0.4, 0.6, 0.8 and 1 g per 20 g of cowpea seeds in test tubes including a control was observed. The treatment materials exhibited significant efficacy from 24 hours after infestation. P. guineense at 1 g concentration significantly reduced the oviposition potential, egg hatching rate, holes and emergence of adult Callosobruchus maculatus on treated seeds. The powders caused chronic toxicity and inhibit development. All the responses were found to be concentration dependent. Increasing rates of P. guineense increased performance. The implication of these results is discussed and recommendations proffered.展开更多
Red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera, Curculionidae), is considered one of the most damaging insect pests of date palms in the Kingdom of Bahrain. Large scale infestation of RPW to da...Red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera, Curculionidae), is considered one of the most damaging insect pests of date palms in the Kingdom of Bahrain. Large scale infestation of RPW to date palm trees leads to excessive feeding activity of the RPW larvae, which is carried out by microorganisms present within RPW and producing a wet fermenting material inside the trunk. Culture dependent-bacteria were isolated from feeding waste and identified by the sequencing of the 16S rRNA gene using 8F and 1492R universal primers. Among the culture-dependent isolated bacteria, 80% were identified by comparing 16S rRNA gene sequence in NCBI database, using BLAST program in GenBank. 85% of the identified bacteria were Gram-positive while the rest of them were Gram-negative. A high abundance of bacteria were from the Bacillaceae family and sixteen different species of Bacillus were identified in comparison with NCBI GenBank. The 16S rRNA gene sequences of identified bacterial strains have been submitted to GenBank. The phylogenetic relationship was studied using 16S rRNA gene sequences, the Gram-negative bacteria came in one clade while Gram-positive different Bacillus sp. and strains showed evolutionary closeness to each other and accordingly, they came in one major clade under three different sub-clades in the phylogenetic tree. The findings of new Bacillus strains in the natural habitat of the date plam trees in the Kingdom of Bahrain, pledge a vast area of research on RPW bio-control research arena.展开更多
The reproductive behavior of red palm weevil, Rhynchophorusferrugineus Olivier was studied in laboratory with several sets of unmated females paired with unmated males for different periods. The treatments in these co...The reproductive behavior of red palm weevil, Rhynchophorusferrugineus Olivier was studied in laboratory with several sets of unmated females paired with unmated males for different periods. The treatments in these controlled mating experiments included single mating, multiple mating for 24 h, multiple mating weekly, multiple mating on alternate days and multiple mating uninterrupted with a control group. During the study, observations were made on the number of eggs laid, incubation period, hatching percentage, ovipositional periods and adult longevity. Though unmated females laid a large number of eggs, these were infertile. This study clearly showed significant differences in the egg-laying capacity of females exposed to different frequencies of mating. The average number of eggs laid varied from 98.9 ± 9.7 eggs in a single-time mated female to 195.8± 24.3 eggs in females allowed to mate on alternate days throughout their life. As far as the percentage of egg-hatching is concerned, there were significant differences in single and multiple mated females with 22.4 ± 3.3% hatching in one time mated females as compared to 85.2 ± 1.9% in alternate day mated females. This study also showed direct impact of female matings on the number of eggs laid and their hatching percentage. Moreover, the unmated and mated female groups showed no significant differences in oviposition and post-ovipositional period and also there was very little difference in male and female adult longevity.展开更多
We estimated quantitative changes to the content of protein and cholesterol in the Haemolymph of adult Red Palm Weevil after being fed on sugar cane treatment with different concentrations of LeucokininlI. In males, 0...We estimated quantitative changes to the content of protein and cholesterol in the Haemolymph of adult Red Palm Weevil after being fed on sugar cane treatment with different concentrations of LeucokininlI. In males, 0.05% has recorded significant increase in total protein, then 0.25% concentration compeer control, while the maximum high of females 4.846 mg at 0.05% compeer control. The effect of leucokininlI on content of haemolymph cholesterol has shown result that 0.05% concentration and have a clear impact on cholesterol concentration for both sexes with an average reduction of 37.989 mg in males compeer with 120.123 for control, and 57.263 in females compeer with 96.087 mg for control.展开更多
The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate (ZnSOa'H20) was toxicologically, biologically and physiologica...The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate (ZnSOa'H20) was toxicologically, biologically and physiologically evaluated as insect development disruptors against the RPW. LCs0 of Zinc sulfate against 10 days old larvae was 0.566%, 3% concentration inhibited the larvae from feeding. LCs0 concentration of zinc sulfate solution was capable to disrupting growth, development and reproduction of R. ferrugineus. Growth and development disruptions are resulted from larval feeding on the treated diet that causing percentage of mortality rate, retardation in larval and pupal duration, percentage of pupation, adults emergence, reduction in deposited eggs that laid by the resulting adults and in the egg fertility. The study further reveals that Zinc sulfate effects on histological structure of epithelial cells of the larval midgut including separation and elongation of their cells, rupture in the peritrophic membrane and in some microvilli and some degeneration of the surrounding muscles. Histological changes also was observed on the adults ovary, in this way, disrupt female gamete production through their action on the accumulation of yolk granules (vitellogenesis) and follicular epithelial cells. Disruption of male gamete production was detected by disorganized of testicular cysts and depopulation of these cysts in addition to degeneration in germ cells. Zinc sulfate may be used among other control methods by injection into the tree trunk or as fertilizer around the tree roots which in turn may have an indirect effect for controlling the RPW.展开更多
基金Project (No. 2002CB111403) supported by the National Basic Re-search and Development Program (973) of China
文摘The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest office Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to flee, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the flee. At tillefing stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2-4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.
文摘Samples of pheromone lures used in boll weevil, Anthonomus grandis (Boheman), eradication programs are routinely analyzed by Gas Chromatography (GC) to ensure lures are adequately dosed with grandlure, the synthetic aggregation phero- mone produced by male weevils. However, preparation of GC samples is tedious, time consuming, and requires a moderate level of experience. We examined the use of a commercially-available electronic nose (e-nose) for rapidly assessing the grandlure contents of lures. The e-nose was trained to recognize headspace collections of grandlure emitted from new lures and after lures were aged under field conditions for 4 d, 7 d, 10 d, and 14 d. Based on cross-validation of the training set, the e-nose was 82% accurate in discriminating among the different age classes of lures. Upon sampling headspace collections of pheromone from a different set of field-aged lures, the e-nose was 〈50% accurate in discriminating 4 d, 7 d, and 10 d aged lures from the other age classes of lures. However, the e-nose identified new and 14 d aged lure samples with 100% accuracy. In light of these findings, e-nose technology shows considerable promise as an alternative approach for rapidly assessing the initial grandlure contents of lures used in boll weevil eradication programs.
基金The financial assistance from the Swedish International Development Agency for some of the study (travel grant for Valantine Mbatchou)
文摘Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Method: The activities were evaluated using standard protocols.In these bioassays, the cowpea seeds were used directly as an insect feed. The activity of the extract and isolated compounds were tested at concentrations of 100, 200 and 300 μg/mL and compared to neem oil and cinnamaldehyde(as standard positive controls). Phytochemical analysis of the ethyl acetate extract was done through a number of chromatographic techniques and the structures of the isolated compounds were established through comprehensive spectroscopic analysis including 2 D-NMR and ESI-MS studies.Results: Fractionation of the active ethyl acetate extract resulted in the isolation of one known anthraquinone,aurantio-obtusin(1) and a novel compound that was named as cassiatorin(2). Compounds1 and 2 showed comparable insect antifeedant properties with the positive controls while their insecticidal and ovipositor deterrent effects were far superior to the standard controls.Conclusions: It is thus concluded that Senna tora extracts and the isolated compounds(1 and2) may be employed in the postharvest management of stored cowpea seeds and as other crop protectants.
基金supported by King Saud University, Deanship of Scientific Research, College of Science Research Center
文摘The Kingdom of Saudi Arabia is one of the leading date producing countries. Unfortunately, this important fruit crop is under great threat from the red palm weevil (RPW) (Rhynchophorus ferrugineus), which is a highly invasive pest. Several techniques, including visual inspection, acoustic sensors, sniffer dogs, and pheromone traps have been tried to detect the early stages of a RPW infestation; however, each method has suffered certain logistical and implementation issues. We have applied laser induced breakdown spectroscopy (LIBS) for the early detection of RPW infestation. Through the analysis of the observed LIBS spectra of different infested and healthy samples, we have found presence of Ca, Mg, Na, C, K elements and OH, CN molecules. The spectra also reveal that with the population growth of the pest, the intensity of Mg and Ca atomic lines in LIBS spectra increases rapidly. Similar behavior is observed in the molecular lines of LIBS spectra. The obtained results indicate that the LIBS technique can be used for the early detection of RPW infestation without damaging the date palms.
文摘The red palm weevil (RPW) Rhynchophorusferrugineus is one of the most invasive insect pests causing massive damage on date palms over the world. Synthetic pyrethroid insecticide beta-cyfluthrin has been evaluated against developmental stages of RPW under laboratory conditions. These stages were exposed to residual film of the insecticide on transparent cups using a Potter precision laboratory spray tower. Percent mortality of males and females were recorded 0.5, 2.0 and 24 h after treatment. Furthermore, date palm offshoots were infested in mesh house, two months later, offshoots treated with 300 ppm (p.g/mL) beta-eyfluthrin using two methods dipping and injection. Bioassay test showed beta-cyfluthrin at 300 ppm caused 86,7% and 93,3% mortality against males and females after 2 h respectively with LC50 135.74 and 109.4 ppm. By increasing the concentration at 400 ppm, mortality reached 100% against males and females after 24 h, Developmental stages, eggs and larvae were more sensitive toward the insecticide, where at 120 ppm no eggs hatched after 96 h and 86.7% mortality was recorded at 200 ppm against larvae after 30 rain with LC50 93.19 ppm. In addition, dipping method of date palm offshoots in 300 ppm beta-cyfluthrin was a very convenient way to control all RPW stages compared to injection method. So, it possible to recommend this method for treating the date palm offshoots in such insecticide as one of the most important elements of integrated pest management for standardizing insecticide based quarantine protocols.
文摘The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), (Coleoptera, Curculionidae, Rhynchophorinae), is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several countries of the Mediterranean Basin during the last three decades where it attacks palm trees. During the last three decades, multiple introductions of RPW to the Middle East, Europe and Caribbean (Island of Curacao, Netherland Antilles), Lebanon and United States of America (Laguna Beach, Orange County, California) (USA) have occurred and the RPW is now a serious pest of many palm species. Duration of all life parameters varies significantly where the entire life cycle takes about 45 to 298 days. Different artificial diets were developed and were tested for mass rearing of RPW. Control of RPW is difficult due to the concealed nature of the life cycle of the pest. Integrated Pest Management (IPM) strategy has been applied successfully to deal with RPW problem. The IPM strategy consists of various preventive and curative methods; those methods were categorized in nine categories in this review. The main objective of this work was to review the existing knowledge on RPW's different aspects, with an ultimate aim of revealing the actual situation of the research on RPW.
文摘Plant diseases generate challenging problems in commercial, agriculture and pose real economic threats to both conventional and organic farming systems. The red palm weevil (Rhynchophorus ferrugineus) (RPW) is one of the most destructive pests of palms in the world. Nowadays, control methods revolve around treatments based on chemicals, biotechnological systems using semi-chemicals or the development of the sterile insect technique (hardly sustainable at this time) and biological control. Biological control as the use of natural microorganisms, extracted products from microorganisms or genetically improved to resist or eliminate of pathogens. Our aim was to evaluate the entomopathogenicity of indigenous Beauveria bassiana and Metarhizium anisopliae obtained in Gaza strip against larvae and adults of R. ferrugineus in order to identify indigenous strains potentially suitable for Red Palm Weevil biological control. B. bassiana & M. anisopliae were isolated from larvae and adult dead of RPW from different position of Gaza strip. Morphological analysis of the isolated fungi and molecular identification was determined using PCR technique. Also, the efficiency of the isolated fungi were evaluated under lab conditions and optimized as a biological agent. On the anther hand, the ability of treated RPW male to infect females is examined and calculated using Abbott's formula. Our results showed that the B. bassiana and M. anisopliae exhibited a good biological control agent against larvae and adults of RPW. The pathogenicity of the two most virulent isolates and the toxicity assay on larvae showed a highest mortality percentage nearly to 100% by 6 days after spraying the larvae with 3.4 × 108 spores/ml of B. bassiana. The mortality percentage reaches to 90% after spraying the larvae with 3.6 × 108 spores/ml of M. anisopliae. The mortality for the adults treated with pesticide arrives to 50% and the control group 10% at the same time. The results revealed that the infection of the adult males by Entomopathogenic fungi (EPF) can be disseminated into the healthy population, after RPW treatment with B. bassiana and M. anisopliae. Our research concludes that B. bassiana and M. anisopliae locally isolated can be used as biological very effective.
文摘The behavior of osier weevil’s adult was studied at Lindian County in Heilongjiang Provillce in 1987-90. Osier weevil started emergence in the last ten days in July and peaked in the tirst and second ten days in August. Emergence on the susceptible species, such as Populus berolinensis, is 5 to 10 days earlier than that on the species of pest-resistance such as P. xiaohei. The adult move slowly, and it spreads or distributes mainly by crawling or by carrying of human beings. Osier weevil has a selection on host species tbr intake nourishment. According to the degree of being addicted, the series can be arranged as Populus berolinensis. Populus staline, Populus pesudo simonii× nigra,Populus xiaohei, Populus pachermis. The adult is likely to dwell on trunks and likely to eat slender and tender branches. Osier weevil makes a choice of host plants to lay eggs. The adult laid more eggs on the P.berolinensis than on P. xiaohei or willow. Most of the eggs laid on the young trees were distributed blow 2 m height. As tree’s age increase, the eggs’ spots get higher and higher. The eggs laid on the eastern and the southern sides of ti1e trunk are more than that laid on the West and the North. P.berolinensis and P. staline are the source trees of osier weevil in artificial Stands in Western Heilongjiang. Cutting the pest source tree, planting pest-resistance species, strengthening the quarantine of the forest plants, and controlling of the diseases and pests timely have very important significance to prevent the occtirrence and damage of this pest.
文摘Pine-cone weevil (Pissodes validirostris Gyll.) is one of the most important pests of Scots pine (Pinus syvestris var. mongolica). The quality and quantity of cone were damaged seriously by this pest. In order to provide a foundation for the control of this pest, the prediction of occurrence date of pine-cone weevil was studied with the methods of time of undergo, the effective accumulative temperture and the phenology forecast. The occurrence quantity was studied on the basis of the rules of the population dynamic and the environmental influences on this pest.
文摘A distribution map of osier weevil (Crytorrynchus lapathi L.) was drown up based on widely collecting information and field survey. The results showed that Osier weevil has a widespread in China, stretching from 33°21' to 51°42' N latitude and 83°00' to 132°58' E longitude, and distributes in forms of big or small patches or sports uncontinously. According to the analysis of meteorological data, the temperature and humidity threshold for osier weevil's distribution were determined by methods of PCA (Principle Component Analysis) and RA(Relativity Analysis): January temperature is -30~ 0℃. Annual temperature -4~13℃, Annual precipitation from 411~ 1,136 min.The areas with January temperature under 0℃, annual temperature above 0℃ and annual precipitation of 400~ 800 mm are the optimum distributing places for osier weevil in China.
基金Supported by the Chinese Academy of Sciences Research Fellowship for International Young Researchers to M.Rajkumar hosted by J.SUNthe Knowledge Innovation Project of the Chinese Academy of Sciences (Nos.KZCX2-YW-QN-205,KZCX2-YW-213-2) to J.SUN
文摘The efficacy of grass carp Ctenopharyngodon idella (Cyprinidae) and weevils Neochetina spp. (Curculionidae) to control the aquatic weed, water hyacinth, is investigated in a square net cage (happas) setting at a farm in Cuddalore District, South India. This novel combination of insects and fish is found to be superior to individual treatments for controlling the weed growth within ll0 d. The biomass of the weed, number of plants, percentage of flowered plants and chlorophyll contents were studied. The weed biomass is reduced from 5 kg (day 1) to 0.33 kg (day 110) when exposed to grass carp and weevils. The number of plants is reduced to 0.75 in grass carp and weevil exposed happas, while it is 741.5 in the control. The mean number of leaves per plant is also reduced. In addition, the chlorophyll a and b are significantly reduced in happas exposed to the combination of fish and insects when compared to the other treatments. Based on the results of this study, we consider the combined use of grass carp and weevils to be more efficient and sustainable for managing water hyacinths than the use of these organisms individually.
文摘Insects of the genus Sitophilus are among the most destructive pests in rice storage, and the best strategy to control it is to use resistant genotypes. In this study, 26 upland rice elite lines were evaluated for Sitophilus weevil reaction on grain weight. The seeds were placed in plastic recipients stored in a room with temperature and lightning simulating a warehouse environment. The number of living weevils and the grain weight were obtained in two evaluations, one 35 days after storage, and the other 35 days after infestation. The lines differed statistically for number of living weevils and for grain weight in both evaluations. The correlations between these two characters were -?0.99 and -?0.47 for the first and the second evaluation (P < 0.05), respectively. The lines BRS Pepita, AB 112089 and AB 112090 were the most susceptible. Seventeen of the twenty-six elite lines were resistant in both evaluations and could be successfully used in upland rice breeding programs.
基金Supported by Guizhou Provincial Key Research Project(QKH NY(2009)3065)~~
文摘[ Objective] Coffee bean weevil (Araecerusfasciculatus De Geer) is a worldwide important pest in storehouse, which distributes in tropical and subtropi- cal region, having overlapping ecological zone with the important biofuel plant Jatropha curcas L.. The paper was to investigate the damage of coffee bean weevil on J. curcas. [Method] Taking planting area in Luodian County of Guizhou Province as the investigation point, the forest stand of J. curcas in field and the indoor stored fruits were investigated, and the occurrence condition and damage consequence of the pest were grasped. Furthermore, the taxonomic status of the pest was also confirmed. [ Result] Coffee bean weevil had common distribution in planting area of J. curcas in Luodian, which was found to cause damage beth in field and indoor condition. The adults of coffee bean weevil fed on fungi with little direct damage on the fruit of J. curcas. However, the adults of the post laid their eggs in- side the peel of fruit, and the larvae hatched and fed inside the peel, resulting in the collapse of the peels, thus causing direct damage on the fruit. In addition, coffee bean weevil might have series of potential damages including direct feeding on seeds, spreading diseases, and posing damage on other economic crops in pro- duction area, etc.. [ Conclusion] J. curcas was an important new host for coffee bean weevil. The pest had certain damage on the plant, which also had potential damage on plant products and other economic crops. The research and control efforts on coffee bean weevil should be strengthened.
文摘Thai rice is favored by large numbers of consumers of all continents because of its excellenttaste,fragrant aroma and fine texture.Among all Thai rice varieties,Thai Hommal rice is themost preferred.Classification of rice as premium quality requires that almost all grain kernels ofthe rice be perfectly whole with only a small quantity of foreign particles.Of all the foreignpartidles found in rice,rice weevils can wreck severest havoc on the quality and quantity of ricesuch thatpremiumitograde rice.It is widely known that ricemillers adoptt the birth and propagation of riceweevils,rice which end up inthebodyweevilscould beapproxilplied and thereby nooverdosantity of rice weevilsinboth Inear infrared spec-TRtroscop)spectrometer was usedinthis researchal 2500 nm.A total of 20 levels of riceweevil infestational mature rice weevils were appliedto 1680 rice samples.The spectral data and quantity of weevils are analyzed by partial leastsquare regression(PLSR)to establish the model for prediction.The results show that the modelis able to estimate the quantity of weevils in milled Hommali rice and brown Hommali rice withhigh Ral of 0.96 and 0.90,high RPD of 6.07 and 3.26 and smalil bias of 2.93 and 2.94,respectively.
基金This research received the support from the Deputyship for Research&Innovation,Ministry of Education in Saudi Arabia through the project number(UB-26-1442).
文摘Smart precision agriculture utilizes modern information and wireless communication technologies to achieve challenging agricultural processes.Therefore,Internet of Things(IoT)technology can be applied to monitor and detect harmful insect pests such as red palm weevils(RPWs)in the farms of date palm trees.In this paper,we propose a new IoT-based framework for early sound detection of RPWs using fine-tuned transfer learning classifier,namely InceptionResNet-V2.The sound sensors,namely TreeVibes devices are carefully mounted on each palm trunk to setup wireless sensor networks in the farm.Palm trees are labeled based on the sensor node number to identify the infested cases.Then,the acquired audio signals are sent to a cloud server for further on-line analysis by our fine-tuned deep transfer learning model,i.e.,InceptionResNet-V2.The proposed infestation classifier has been successfully validated on the public TreeVibes database.It includes total short recordings of 1754 samples,such that the clean and infested signals are 1754 and 731 samples,respectively.Compared to other deep learning models in the literature,our proposed InceptionResNet-V2 classifier achieved the best performance on the public database of TreeVibes audio recordings.The resulted classification accuracy score was 97.18%.Using 10-fold cross validation,the fine-tuned InceptionResNet-V2 achieved the best average accuracy score and standard deviation of 94.53%and±1.69,respectively.Applying the proposed intelligent IoT-aided detection system of RPWs in date palm farms is the main prospect of this research work.
文摘The Central Bank of Nigeria Anchored Borrowers Programme efforts geared towards massive production of cowpea and sustainable food security is challenged with post-harvest losses especially due to insect pest infestation in storage. So also, chemical method of pest control posed more health and ecological challenges than food insecurity. This work investigated the effect of Piper guineense on cowpea storage weevil at the agronomy laboratory of the Lagos State Polytechnic, Ikorodu. Contact toxicity of P. guineense at 0.2, 0.4, 0.6, 0.8 and 1 g per 20 g of cowpea seeds in test tubes including a control was observed. The treatment materials exhibited significant efficacy from 24 hours after infestation. P. guineense at 1 g concentration significantly reduced the oviposition potential, egg hatching rate, holes and emergence of adult Callosobruchus maculatus on treated seeds. The powders caused chronic toxicity and inhibit development. All the responses were found to be concentration dependent. Increasing rates of P. guineense increased performance. The implication of these results is discussed and recommendations proffered.
文摘Red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera, Curculionidae), is considered one of the most damaging insect pests of date palms in the Kingdom of Bahrain. Large scale infestation of RPW to date palm trees leads to excessive feeding activity of the RPW larvae, which is carried out by microorganisms present within RPW and producing a wet fermenting material inside the trunk. Culture dependent-bacteria were isolated from feeding waste and identified by the sequencing of the 16S rRNA gene using 8F and 1492R universal primers. Among the culture-dependent isolated bacteria, 80% were identified by comparing 16S rRNA gene sequence in NCBI database, using BLAST program in GenBank. 85% of the identified bacteria were Gram-positive while the rest of them were Gram-negative. A high abundance of bacteria were from the Bacillaceae family and sixteen different species of Bacillus were identified in comparison with NCBI GenBank. The 16S rRNA gene sequences of identified bacterial strains have been submitted to GenBank. The phylogenetic relationship was studied using 16S rRNA gene sequences, the Gram-negative bacteria came in one clade while Gram-positive different Bacillus sp. and strains showed evolutionary closeness to each other and accordingly, they came in one major clade under three different sub-clades in the phylogenetic tree. The findings of new Bacillus strains in the natural habitat of the date plam trees in the Kingdom of Bahrain, pledge a vast area of research on RPW bio-control research arena.
文摘The reproductive behavior of red palm weevil, Rhynchophorusferrugineus Olivier was studied in laboratory with several sets of unmated females paired with unmated males for different periods. The treatments in these controlled mating experiments included single mating, multiple mating for 24 h, multiple mating weekly, multiple mating on alternate days and multiple mating uninterrupted with a control group. During the study, observations were made on the number of eggs laid, incubation period, hatching percentage, ovipositional periods and adult longevity. Though unmated females laid a large number of eggs, these were infertile. This study clearly showed significant differences in the egg-laying capacity of females exposed to different frequencies of mating. The average number of eggs laid varied from 98.9 ± 9.7 eggs in a single-time mated female to 195.8± 24.3 eggs in females allowed to mate on alternate days throughout their life. As far as the percentage of egg-hatching is concerned, there were significant differences in single and multiple mated females with 22.4 ± 3.3% hatching in one time mated females as compared to 85.2 ± 1.9% in alternate day mated females. This study also showed direct impact of female matings on the number of eggs laid and their hatching percentage. Moreover, the unmated and mated female groups showed no significant differences in oviposition and post-ovipositional period and also there was very little difference in male and female adult longevity.
文摘We estimated quantitative changes to the content of protein and cholesterol in the Haemolymph of adult Red Palm Weevil after being fed on sugar cane treatment with different concentrations of LeucokininlI. In males, 0.05% has recorded significant increase in total protein, then 0.25% concentration compeer control, while the maximum high of females 4.846 mg at 0.05% compeer control. The effect of leucokininlI on content of haemolymph cholesterol has shown result that 0.05% concentration and have a clear impact on cholesterol concentration for both sexes with an average reduction of 37.989 mg in males compeer with 120.123 for control, and 57.263 in females compeer with 96.087 mg for control.
文摘The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate (ZnSOa'H20) was toxicologically, biologically and physiologically evaluated as insect development disruptors against the RPW. LCs0 of Zinc sulfate against 10 days old larvae was 0.566%, 3% concentration inhibited the larvae from feeding. LCs0 concentration of zinc sulfate solution was capable to disrupting growth, development and reproduction of R. ferrugineus. Growth and development disruptions are resulted from larval feeding on the treated diet that causing percentage of mortality rate, retardation in larval and pupal duration, percentage of pupation, adults emergence, reduction in deposited eggs that laid by the resulting adults and in the egg fertility. The study further reveals that Zinc sulfate effects on histological structure of epithelial cells of the larval midgut including separation and elongation of their cells, rupture in the peritrophic membrane and in some microvilli and some degeneration of the surrounding muscles. Histological changes also was observed on the adults ovary, in this way, disrupt female gamete production through their action on the accumulation of yolk granules (vitellogenesis) and follicular epithelial cells. Disruption of male gamete production was detected by disorganized of testicular cysts and depopulation of these cysts in addition to degeneration in germ cells. Zinc sulfate may be used among other control methods by injection into the tree trunk or as fertilizer around the tree roots which in turn may have an indirect effect for controlling the RPW.