鸡蛋清中卵转铁蛋白的分离提取研究

为了研究鸡蛋清中卵转铁蛋白的分离提取工艺，采用硫酸铵沉淀和离子交换层析分离卵转铁蛋白，用聚丙烯酰胺凝胶电泳（SDS—PAGE）检测其纯度。鸡蛋清原液用Tris—HCl缓冲液稀释5倍，4℃下静置6h以上，采用50％～80％硫酸铵分级沉淀和Q—Sepharose FF离子交换层析分离卵转铁蛋白。结果显示，分离提取的卵转铁蛋白纯度达到96％，回收率为88．63％，且其具有生物活性，对大肠杆菌的抑菌率为48．84％。表明采用硫酸铵分级沉淀和Q—SepharoseFF离子交换层析法分离鸡蛋清中卵转铁蛋白可行。

鸡蛋清中3种蛋白质的连续化提取工艺初步研究

研究鸡蛋清中溶菌酶、卵转铁蛋白和卵白蛋白的分离提取工艺。采用硫酸铵沉淀和离子交换层析分离3种蛋白质,并用聚丙烯酰胺凝胶电泳(SDS-PAGE)检测其纯度。结果显示,层析纯化后SDS-PAGE检测其纯度达到电泳纯,溶菌酶的比活由144.13 U/mg提高到2 235 U/mg,纯化倍数达15倍,回收率为15.76%;卵转铁蛋白抑菌率为48.84%。该方法分离溶菌酶、卵转铁蛋白和卵白蛋白的工艺路线简单、快捷、成本低,适用于工业生产。

Preliminary Study on the Separation of Lysozyme,Ovotransferrin and Ovalbumin from Egg White

[Objective] A study on separation process of lysozyme, ovotransferrin and ovalbumin from egg white. [Method] The proteins were separated by ammonium sul-fates and ion-exchange chromatography. Purity of the proteins was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. [Result] The results showed that the proteins were electrophoresis-pure. The specific activity of lysozyme was increased from 144.13 to 2 235 U/mg, and purification factor was 15-fold. Lysozyme recovery rate was estimated to be 15.76%. Bacteriostasis rate of ovotransferrin was 48.84%. [Conclusion] The procedure for separating lysozyme, ovotransferrin and ovalbumin from egg white was simple, fast, low-cost and suitable for industrilization.