This study aimed to investigate the impact of developmental endothelial locus-1(Del-1)on sarcoplasmic reticulum Ca……(2+)ATPase 2(SERCA2)and its potential effects on spinal cord injury(SCI).A total of 48 mice were ra...This study aimed to investigate the impact of developmental endothelial locus-1(Del-1)on sarcoplasmic reticulum Ca……(2+)ATPase 2(SERCA2)and its potential effects on spinal cord injury(SCI).A total of 48 mice were randomly assigned to the sham group,SCI group,and SCI+CE group.Each group was further divided into two subgroups.The Del treatment subgroup received tail-vein injections of Del-1(1μg/d)for a consecutive week,while the other group was injected with an equivalent volume of normal saline.After 1 week of experimentation,the mice were euthanized,and the spinal cord was extracted for further analysis.Initially,the impact of Del-1 on SERCA2 in the spinal cord was assessed using western blotting analysis.Subsequently,the effects of Del-1 on stress,inflammation,and apoptosis in the endoplasmic reticulum(ER)of SCI mice were analyzed through Western blotting analysis and fluorescent TUNEL.Finally,the study utilized Western blotting analysis and fluorescent TUNEL analysis to examine the consequences of Del-1 blocking SERCA2 on ER stress,inflammation,and apoptosis in mice.The results revealed that Del-1 treatment significantly increased the expression of SERCA2 in the spinal cord(P<0.01)and mitigated SCI-induced ER stress,inflammation,and neuronal apoptosis(P<0.01).Blocking SERCA2 expression in the spinal cord of SCI mice promoted ER stress,inflammatory response,and neuronal cell apoptosis(P<0.01).However,Del-1 treatment did not alleviate the effects of blocking SERCA2 on ER stress,inflammatory response,and neuronal cell apoptosis(P>0.05).In conclusion,this study proposed that Del-1 could reduce ER stress,inflammatory response,and nerve cell apoptosis in spinal cord injury by enhancing the expression of SERCA2.展开更多
目的系统评价糖皮质激素、对乙酰氨基酚及抗菌药物应用于分娩镇痛产时发热的临床疗效及安全性。方法计算机检索PubMed、Embase、Cochrane Library、Web of Science、CBM、VIP和CNKI数据库,搜集糖皮质激素、对乙酰氨基酚及抗菌药物应用...目的系统评价糖皮质激素、对乙酰氨基酚及抗菌药物应用于分娩镇痛产时发热的临床疗效及安全性。方法计算机检索PubMed、Embase、Cochrane Library、Web of Science、CBM、VIP和CNKI数据库,搜集糖皮质激素、对乙酰氨基酚及抗菌药物应用于分娩镇痛产时发热的随机对照试验(randomized controlled trial,RCT),检索时限均从建库至2023年6月30日。由2位评价员独立筛选文献、提取资料并评价纳入文献的偏倚风险后,采用RevMan 5.4软件进行Meta分析。结果共纳入10个RCT,包括1337名产妇。Meta分析结果显示:与对照组相比,应用糖皮质激素可降低分娩镇痛产妇产时发热发生率[OR=0.52,95%CI(0.33,0.82),P<0.01],但使用对乙酰氨基酚和抗菌药物与对照组的差异无统计学意义。结论当前证据显示,应用糖皮质激素可降低分娩镇痛产妇产时发热发生率,但使用对乙酰氨基酚及抗菌药物未能降低产妇产时发热的发生率。受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。展开更多
基金National Key Research and Development Plan of China(Grant No.2016YFC1101503)。
文摘This study aimed to investigate the impact of developmental endothelial locus-1(Del-1)on sarcoplasmic reticulum Ca……(2+)ATPase 2(SERCA2)and its potential effects on spinal cord injury(SCI).A total of 48 mice were randomly assigned to the sham group,SCI group,and SCI+CE group.Each group was further divided into two subgroups.The Del treatment subgroup received tail-vein injections of Del-1(1μg/d)for a consecutive week,while the other group was injected with an equivalent volume of normal saline.After 1 week of experimentation,the mice were euthanized,and the spinal cord was extracted for further analysis.Initially,the impact of Del-1 on SERCA2 in the spinal cord was assessed using western blotting analysis.Subsequently,the effects of Del-1 on stress,inflammation,and apoptosis in the endoplasmic reticulum(ER)of SCI mice were analyzed through Western blotting analysis and fluorescent TUNEL.Finally,the study utilized Western blotting analysis and fluorescent TUNEL analysis to examine the consequences of Del-1 blocking SERCA2 on ER stress,inflammation,and apoptosis in mice.The results revealed that Del-1 treatment significantly increased the expression of SERCA2 in the spinal cord(P<0.01)and mitigated SCI-induced ER stress,inflammation,and neuronal apoptosis(P<0.01).Blocking SERCA2 expression in the spinal cord of SCI mice promoted ER stress,inflammatory response,and neuronal cell apoptosis(P<0.01).However,Del-1 treatment did not alleviate the effects of blocking SERCA2 on ER stress,inflammatory response,and neuronal cell apoptosis(P>0.05).In conclusion,this study proposed that Del-1 could reduce ER stress,inflammatory response,and nerve cell apoptosis in spinal cord injury by enhancing the expression of SERCA2.
