目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清...目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。展开更多
目的:分析妊娠期亚临床甲状腺功能减退症(SCH)孕妇血清可溶性白细胞介素-2受体(SIL-2R)、同型半胱氨酸(HCY)的表达意义及与妊娠结局的关系。方法:选择我院自2021年1月至2023年6月收治的120例妊娠期SCH孕妇(观察组)及120例正常妊娠孕妇(...目的:分析妊娠期亚临床甲状腺功能减退症(SCH)孕妇血清可溶性白细胞介素-2受体(SIL-2R)、同型半胱氨酸(HCY)的表达意义及与妊娠结局的关系。方法:选择我院自2021年1月至2023年6月收治的120例妊娠期SCH孕妇(观察组)及120例正常妊娠孕妇(对照组);检测两组血清SIL-2R、HCY表达水平,分析血清SIL-2R、HCY与促甲状腺激素(TSH)的关系,通过受试者工作特征曲线(ROC)下面积(AUC)评价观察组血清SIL-2R联合HCY对妊娠结局不良的预测效能。结果:观察组妊娠期碘摄入量偏少比例、TPOAb阳性率和TGAb阳性率均高于对照组,差异均有统计学意义(P Objective: To analyze the expression significance of serum soluble interleukin-2 receptor (SIL-2R) and homocysteine (HCY) in pregnant women with subclinical hypothyroidism (SCH) during pregnancy and their relationship with pregnancy outcome. Methods: 120 SCH pregnant women (observation group) and 120 normal pregnant women (control group) admitted to our hospital from January 2021 to June 2023 were selected. The expression levels of serum SIL-2R and HCY in the two groups were detected, and the relationship between serum SIL-2R and HCY and thyroid stimulating hormone (TSH) was analyzed. The predictive efficacy of serum SIL-2R combined with HCY in the observation group was evaluated by area under receiver operating characteristic curve (ROC) (AUC). Results: The proportion of low iodine intake, TPOAb positive rate and TGAb positive rate in the observation group were higher than those in the control group, and the differences were statistically significant (P < 0.05). The expression levels of serum SIL-2R and HCY in observation group were higher than those in control group, and the differences were statistically significant (P < 0.05). By Pearson correlation analysis, serum SIL-2R and HCY expression levels of SCH pregnant women were positively correlated with TSH (P < 0.05). Logistic regression analysis showed that low iodine intake and high levels of serum SIL-2R and HCY were independent influencing factors of SCH during pregnancy (P < 0.05). The levels of serum SIL-2R and HCY in the adverse pregnancy outcome group were higher than those in the normal pregnancy outcome group, with statistical significance (P < 0.05). ROC curve analysis showed that the specificity, sensitivity and AUC of serum SIL-2R combined with HCY in predicting adverse pregnancy outcomes of SCH pregnant women were 62.81%, 88.69% and 0.931 respectively. Conclusion: The increase of serum SIL-2R and HCY expression levels is closely related to the occurrence of SCH during pregnancy, and the combination of the two can predict the adverse pregnancy outcome of pregnant women, which is worthy of clinical attention.展开更多
文摘目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。
文摘目的:分析妊娠期亚临床甲状腺功能减退症(SCH)孕妇血清可溶性白细胞介素-2受体(SIL-2R)、同型半胱氨酸(HCY)的表达意义及与妊娠结局的关系。方法:选择我院自2021年1月至2023年6月收治的120例妊娠期SCH孕妇(观察组)及120例正常妊娠孕妇(对照组);检测两组血清SIL-2R、HCY表达水平,分析血清SIL-2R、HCY与促甲状腺激素(TSH)的关系,通过受试者工作特征曲线(ROC)下面积(AUC)评价观察组血清SIL-2R联合HCY对妊娠结局不良的预测效能。结果:观察组妊娠期碘摄入量偏少比例、TPOAb阳性率和TGAb阳性率均高于对照组,差异均有统计学意义(P Objective: To analyze the expression significance of serum soluble interleukin-2 receptor (SIL-2R) and homocysteine (HCY) in pregnant women with subclinical hypothyroidism (SCH) during pregnancy and their relationship with pregnancy outcome. Methods: 120 SCH pregnant women (observation group) and 120 normal pregnant women (control group) admitted to our hospital from January 2021 to June 2023 were selected. The expression levels of serum SIL-2R and HCY in the two groups were detected, and the relationship between serum SIL-2R and HCY and thyroid stimulating hormone (TSH) was analyzed. The predictive efficacy of serum SIL-2R combined with HCY in the observation group was evaluated by area under receiver operating characteristic curve (ROC) (AUC). Results: The proportion of low iodine intake, TPOAb positive rate and TGAb positive rate in the observation group were higher than those in the control group, and the differences were statistically significant (P < 0.05). The expression levels of serum SIL-2R and HCY in observation group were higher than those in control group, and the differences were statistically significant (P < 0.05). By Pearson correlation analysis, serum SIL-2R and HCY expression levels of SCH pregnant women were positively correlated with TSH (P < 0.05). Logistic regression analysis showed that low iodine intake and high levels of serum SIL-2R and HCY were independent influencing factors of SCH during pregnancy (P < 0.05). The levels of serum SIL-2R and HCY in the adverse pregnancy outcome group were higher than those in the normal pregnancy outcome group, with statistical significance (P < 0.05). ROC curve analysis showed that the specificity, sensitivity and AUC of serum SIL-2R combined with HCY in predicting adverse pregnancy outcomes of SCH pregnant women were 62.81%, 88.69% and 0.931 respectively. Conclusion: The increase of serum SIL-2R and HCY expression levels is closely related to the occurrence of SCH during pregnancy, and the combination of the two can predict the adverse pregnancy outcome of pregnant women, which is worthy of clinical attention.
