Diagnosis of Colibacillosis in Foxes

An epidemic occurred in a fox farm of Changli County in April, 2015. The clinical symptoms in ill foxes included depression, poor appetite or appetite loss,rough, disorder and dull hair, standing inability, trembling, sunken eyes, decreased skin elasticity, dehydration and loose stools around the anus. When the abdomens of ill foxes were pressed, white or yellow manure flew out from the anuses, and some of the manures contained mucus or blood. The pathological changes included liver enlargement with necrotic lesions, spleen enlargement, lung hemorrhage, kidney enlargement with bleeding spots, thymus hemorrhage and intestinal catarrhal inflammation. The epidemic was finally identified as colibacillosis through clinical symptoms observation, pathological anatomy, biochemical tests and 16 SrRNA amplification.

Histopathological Observation of Canine Distemper in Raccoon Dogs

In this study, histopathological changes in the heart, liver, spleen, lung, kidney and other organs of raccoon dogs died of canine distemper were observed. According to the results, the lung of infected raccoon dogs exhibited severe hemor- rhage and emphysema; the liver was congested and degenerated; the spleen was enlarged; the exhibited severe hemorrhage and hyaline degeneration. Multiple organs of infected raccoon dogs were congested and hemorrhaged with tissue damage, in- flammatory cell infiltration and a series of pathological changes. This study laid a solid foundation for clinical diagnosis and treatment of canine distemper in raccoon dogs.

猪源大肠杆菌tem耐药基因的检测

为了解和掌握大肠杆菌耐药性的流行情况,从而为控制大肠杆菌的耐药性提供科学依据,用K-B法对临床分离的15株猪源大肠杆菌进行β内酰胺类抗生素药敏试验,以15株分离菌DNA为模板对β内酰胺类抗生素耐药基因tem进行PCR扩增。结果表明:15株猪源大肠杆菌对β内酰胺类抗生素均耐药,耐药率从高到低分别为头孢哌酮(82%)、头孢噻肟(24%)、头孢他啶(18%)、头孢呋辛(6%)、苯唑西林(6%);PCR扩增均检测到β内酰胺类抗生素耐药基因tem。说明β内酰胺类抗生素耐药基因tem广泛存在于猪源大肠杆菌中,与抗生素敏感性表型一致。