通过回顾性分析4例自体颅骨修补术后出现硬膜外/皮下积液患者的临床资料和处理方式,总结修补后并发症发生原因,探讨自体颅骨修补术后出现硬膜外/皮下积液的原因及相应的防治措施。4例患者在自体颅骨修补术后均出现了硬膜外/皮下积液,经...通过回顾性分析4例自体颅骨修补术后出现硬膜外/皮下积液患者的临床资料和处理方式,总结修补后并发症发生原因,探讨自体颅骨修补术后出现硬膜外/皮下积液的原因及相应的防治措施。4例患者在自体颅骨修补术后均出现了硬膜外/皮下积液,经过一段时间的治疗后均已吸收,术后恢复情况可。皮下积液是颅骨修补后最常见的并发症之一,产生的原因也多种多样,结合查阅的相关文献,手术中的止血不充分、术中颅板和脑膜悬吊过深导致的脑脊液漏、手术部位的差异、自体颅骨的不当保存方式等多种因素等均是发生硬膜外/皮下积液的原因。All 4 patients developed epidural/subcutaneous effusion after autologous cranioplasty, which was absorbed after a period of treatment with good outcome. Subcutaneous effusion is one of the common complications after autologous cranioplasty. In adequate haemostasis during surgery, cerebrospinal fluid leakage due to appropriate suspension of dura, and differences in surgical sites are some of the reasons for the occurrence of epidural/subcutaneous effusion. The optimal autologous cranial bone preservation techniques, the appropriate suspension of dura and temporalis, effective haemostasis and internal environment homeostasis in perioprative period can reduce the incidence of epidural/subcutaneous effusions after autologous cranioplasty.展开更多
目的探讨锌指蛋白A20(zinc finger protein A20)对脂多糖(LPS)诱导的大鼠腹膜间皮细胞(RPMCs)炎症效应的影响及可能机制。方法分离及培养RPMCs,常规传代及鉴定。取第2代细胞用于实验研究。将细胞随机分成对照组、LPS组、转染A20组、空...目的探讨锌指蛋白A20(zinc finger protein A20)对脂多糖(LPS)诱导的大鼠腹膜间皮细胞(RPMCs)炎症效应的影响及可能机制。方法分离及培养RPMCs,常规传代及鉴定。取第2代细胞用于实验研究。将细胞随机分成对照组、LPS组、转染A20组、空载体组。脂质体转染A20质粒(pGEM-Teasy-A20)至RPMCs12h后分别在LPS刺激不同时间点收获细胞提取蛋白及细胞上清液。用Western blotting检测细胞TLR4、p-IκBα、IκBα蛋白的表达;用ELISA法检测培养上清液IL-18蛋白水平。结果 LPS刺激8h后,转染A20组RPMCsTLR4蛋白表达水平无明显增高,与对照组相比,P=0.223;与LPS组及空载体组相比,差异有显著性(P=0.003,0.002)。LPS刺激1h后,转染A20组RPMCsp-IκBα蛋白无明显降解,p-IκBα/IκBα比值与对照组相比,P=0.553。与LPS组及空载体组相比,差异有显著性(P=0.001,0.001)。在LPS刺激12h后,转染A20组RPMCsIL-18蛋白分泌水平(479.12±85.79)pg/ml高于对照组(274.34±47.21)pg/ml(P=0.012),但明显低于LPS组(1049.45±185.01)pg/ml及空载体组(1028.77±192.90)pg/ml(P=0.011,0.015)。结论 A20通过对LPS信号通路中多个相关功能蛋白的负性调控作用,阻抑LPS诱导的RPMCs炎症效应。展开更多
文摘通过回顾性分析4例自体颅骨修补术后出现硬膜外/皮下积液患者的临床资料和处理方式,总结修补后并发症发生原因,探讨自体颅骨修补术后出现硬膜外/皮下积液的原因及相应的防治措施。4例患者在自体颅骨修补术后均出现了硬膜外/皮下积液,经过一段时间的治疗后均已吸收,术后恢复情况可。皮下积液是颅骨修补后最常见的并发症之一,产生的原因也多种多样,结合查阅的相关文献,手术中的止血不充分、术中颅板和脑膜悬吊过深导致的脑脊液漏、手术部位的差异、自体颅骨的不当保存方式等多种因素等均是发生硬膜外/皮下积液的原因。All 4 patients developed epidural/subcutaneous effusion after autologous cranioplasty, which was absorbed after a period of treatment with good outcome. Subcutaneous effusion is one of the common complications after autologous cranioplasty. In adequate haemostasis during surgery, cerebrospinal fluid leakage due to appropriate suspension of dura, and differences in surgical sites are some of the reasons for the occurrence of epidural/subcutaneous effusion. The optimal autologous cranial bone preservation techniques, the appropriate suspension of dura and temporalis, effective haemostasis and internal environment homeostasis in perioprative period can reduce the incidence of epidural/subcutaneous effusions after autologous cranioplasty.
